##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241015
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01682882808124	33.0	31.0	34.0	30.0	34.0
2	32.140269277845775	33.0	31.0	34.0	30.0	34.0
3	32.10346244009709	33.0	31.0	34.0	30.0	34.0
4	35.72076841690351	37.0	35.0	37.0	33.0	37.0
5	31.865356927992035	37.0	35.0	37.0	0.0	37.0
6	33.7475592805427	37.0	35.0	37.0	17.0	37.0
7	35.430894342675764	37.0	35.0	37.0	32.0	37.0
8	35.67093334439765	37.0	35.0	37.0	33.0	37.0
9	37.5996307283779	39.0	37.0	39.0	35.0	39.0
10	37.29847934775844	39.0	37.0	39.0	34.0	39.0
11	37.255345103001886	39.0	37.0	39.0	34.0	39.0
12	37.03132170196876	39.0	35.0	39.0	34.0	39.0
13	36.840951807978755	39.0	35.0	39.0	33.0	39.0
14	38.0309980706595	40.0	37.0	41.0	33.0	41.0
15	38.15882828869572	40.0	37.0	41.0	33.0	41.0
16	38.20077588531834	40.0	37.0	41.0	34.0	41.0
17	38.1550733356845	40.0	36.0	41.0	34.0	41.0
18	38.12895462937991	40.0	36.0	41.0	34.0	41.0
19	38.03163703503931	40.0	36.0	41.0	34.0	41.0
20	37.9975146775097	40.0	36.0	41.0	34.0	41.0
21	37.99757276518059	40.0	36.0	41.0	34.0	41.0
22	38.05891749476174	40.0	36.0	41.0	34.0	41.0
23	38.02208576229695	40.0	36.0	41.0	34.0	41.0
24	38.02542165425388	40.0	36.0	41.0	34.0	41.0
25	37.82019791299297	40.0	35.0	41.0	34.0	41.0
26	37.81138518349481	40.0	35.0	41.0	34.0	41.0
27	37.744559467253076	40.0	35.0	41.0	33.0	41.0
28	37.692683028027304	40.0	36.0	41.0	33.0	41.0
29	37.672895877849925	40.0	36.0	41.0	33.0	41.0
30	37.56162894425658	40.0	36.0	41.0	33.0	41.0
31	37.50430056220567	40.0	35.0	41.0	33.0	41.0
32	37.381723129265815	40.0	35.0	41.0	33.0	41.0
33	37.32519137812999	40.0	35.0	41.0	33.0	41.0
34	37.23395639275564	40.0	35.0	41.0	33.0	41.0
35	37.25518328734726	40.0	35.0	41.0	33.0	41.0
36	37.134493703711385	40.0	35.0	41.0	33.0	41.0
37	37.028114432711654	40.0	35.0	41.0	32.0	41.0
38	36.887899093417424	39.0	35.0	41.0	32.0	41.0
39	36.865489699811214	39.0	35.0	41.0	32.0	41.0
40	36.808995290749536	39.0	35.0	41.0	31.0	41.0
41	36.73535672053607	39.0	35.0	41.0	31.0	41.0
42	36.765856896873636	39.0	35.0	41.0	32.0	41.0
43	36.65579320789163	39.0	35.0	41.0	31.0	41.0
44	36.62917245814576	39.0	35.0	41.0	31.0	41.0
45	36.62234715681596	39.0	35.0	41.0	31.0	41.0
46	36.52973466381761	39.0	35.0	41.0	31.0	41.0
47	36.361301163827974	39.0	35.0	41.0	31.0	41.0
48	36.37025911250337	39.0	35.0	41.0	31.0	41.0
49	36.39606663485675	39.0	35.0	41.0	31.0	41.0
50	36.27555131423355	38.0	35.0	41.0	31.0	41.0
51	35.27110760741033	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	24.0
11	5.0
12	9.0
13	5.0
14	9.0
15	8.0
16	15.0
17	26.0
18	59.0
19	107.0
20	198.0
21	434.0
22	740.0
23	1034.0
24	1367.0
25	1554.0
26	1670.0
27	1938.0
28	2145.0
29	2505.0
30	3253.0
31	4308.0
32	6092.0
33	9395.0
34	16014.0
35	23757.0
36	17984.0
37	27174.0
38	44364.0
39	74741.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.287098313382984	18.4905503806817	22.596103976930895	11.62624732900442
2	30.839159388419805	27.42360433997884	24.128373752670996	17.60886251893036
3	31.876439225774327	27.231500114100783	24.697218015476217	16.194842644648674
4	27.975852125386385	24.02837997635002	29.28157998464826	18.714187913615334
5	24.853639814949275	35.2612907910296	21.25261913158932	18.6324502624318
6	23.015165031222125	37.82420181316515	24.049540485032054	15.11109267058067
7	76.52220816131776	5.6934215712714975	12.971806734020705	4.812563533390038
8	76.21475841752589	8.71315063377798	9.021845113374686	6.050245835321453
9	71.2349853743543	6.2734684563201455	10.969026824056593	11.522519345268966
10	42.984876459971375	23.192747339377217	17.810509719312076	16.011866481339336
11	33.13237765284318	22.935501939713294	23.892288861689106	20.039831545754414
12	28.564612161068816	20.258075223533805	29.832168122316038	21.345144493081346
13	25.32456486110823	25.685953156442544	32.21500736468684	16.77447461776238
14	19.07308673734	32.30255378295956	28.092857291039973	20.531502188660458
15	16.772400058087673	24.452419973860547	38.950687716532165	19.824492251519615
16	19.155239300458476	22.343007696616393	33.24481878721242	25.256934215712718
17	19.10047092504616	23.272825342820987	29.927597867352656	27.6991058647802
18	20.814887040225713	23.341700724021326	31.137066157708027	24.706346078044934
19	22.404414662987783	25.14200360973383	27.959255647988712	24.49432607928967
20	24.32421218596353	24.69182416032197	29.182001120262225	21.801962533452272
21	23.086944795967057	27.73146899570566	28.219820343132167	20.96176586519511
22	20.42196543783582	23.573636495653798	28.785345310457856	27.219052756052527
23	20.857208057589776	27.562599838184344	27.64392257743294	23.936269526792938
24	22.206086758085597	23.940003734207416	29.485716656639628	24.36819285106736
25	20.017011389332616	27.692882185756073	28.44885173122005	23.841254693691262
26	19.219550650374458	25.558990104350354	30.528390349148392	24.693068896126796
27	22.837997635001972	26.717839138642823	27.258469389871998	23.185693836483207
28	18.09638404248698	27.200796630915086	31.916270771528744	22.786548555069185
29	20.124473580482544	24.894301184573575	31.367757193535674	23.61346804140821
30	23.501441818973923	26.328236831732465	28.87330664066552	21.297014708628094
31	24.163226355206106	26.44150778997158	23.73005829512686	25.665207559695453
32	24.59307511980582	29.32390100201232	24.843681928510676	21.239341949671182
33	25.27643507665498	25.309213119515384	24.461548036429267	24.95280376740037
34	21.047652635728067	25.822459183038397	27.379208762940067	25.750679418293466
35	22.97118436611829	24.314254299524926	25.820799535298633	26.89376179905815
36	24.79015828890318	27.063045868514408	26.630292720370104	21.51650312221231
37	22.00983341285812	28.006555608572082	27.21033960541875	22.77327137315105
38	21.65176441300334	29.868265460655973	23.04420886666805	25.435761259672635
39	22.228906914507395	26.59295064622534	25.8631205526627	25.315021886604566
40	24.066136962429724	22.061697404725848	27.661763790635437	26.21040184220899
41	19.061054291226686	25.952326618675187	26.599174325249464	28.387444764848663
42	21.37460324046221	25.55857519241541	27.880422380349774	25.186399186772608
43	23.34958405078522	26.966371387672964	25.57019272659378	24.113851834948033
44	22.12185963529241	25.232039499616203	27.942244258656103	24.703856606435284
45	21.3268883679439	25.02707300375495	26.27471319212497	27.371325436176168
46	25.349044665269798	25.37767358878078	26.63485675165446	22.63842499429496
47	20.126133228222308	25.02375370827542	30.815094496193186	24.035018567309088
48	21.189552517478166	25.394684978113396	27.35721843038815	26.05854407402029
49	21.059270169906437	23.401033130718005	31.06072236167873	24.478974337696823
50	21.84967740597058	23.738356533825698	28.95628902765388	25.45567703254984
51	20.740202891936185	23.812210858245336	25.768520631495967	29.679065618322507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	278.0
2	451.0
3	705.0
4	959.0
5	690.0
6	421.0
7	446.0
8	471.0
9	455.0
10	439.0
11	452.5
12	466.0
13	445.0
14	424.0
15	400.5
16	377.0
17	398.5
18	420.0
19	418.0
20	416.0
21	503.0
22	590.0
23	629.5
24	669.0
25	794.5
26	1035.5
27	1151.0
28	1552.5
29	1954.0
30	2188.5
31	2423.0
32	3067.5
33	3712.0
34	4109.0
35	4506.0
36	5286.5
37	6067.0
38	6641.5
39	7216.0
40	8626.5
41	10037.0
42	11716.0
43	13395.0
44	15632.5
45	17870.0
46	24502.0
47	31134.0
48	28965.0
49	26796.0
50	26378.0
51	25960.0
52	22422.5
53	18885.0
54	16285.0
55	13685.0
56	12416.5
57	11148.0
58	10264.5
59	9381.0
60	8733.5
61	8086.0
62	7307.5
63	6529.0
64	5671.0
65	4813.0
66	3984.5
67	3156.0
68	2770.5
69	2385.0
70	1803.5
71	1222.0
72	1242.0
73	1262.0
74	903.0
75	428.0
76	312.0
77	227.0
78	142.0
79	110.0
80	78.0
81	45.0
82	12.0
83	12.0
84	12.0
85	8.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.809866605812914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39237398238316	25.54778748210692
2	10.382969593668427	7.228595730556189
3	4.007294658927018	4.1848017758230815
4	2.0656280915884953	2.876169533016617
5	1.2682217480958795	2.207331493890422
6	0.8617709810839482	1.7998879737775655
7	0.6722528815094699	1.6380723191502606
8	0.5482913572594967	1.5268759205858555
9	0.5029977233989297	1.575835528908989
>10	6.188540710633276	43.20021575420617
>50	0.06436463759133222	1.5235566251063213
>100	0.03695006972835739	2.6201688691575216
>500	0.003575813199518457	0.7028608177914237
>1k	0.004767750932691276	3.36784017592266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	2480	1.0289815986556852	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	2266	0.9401904445781383	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	2051	0.8509843785656495	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	1320	0.5476837541231874	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	634	0.26305416675310667	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	552	0.22903138808787835	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	508	0.21077526295043875	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT	456	0.18919984233346474	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	419	0.1738481007406178	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	411	0.17052880526108333	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA	396	0.1643051262369562	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC	337	0.1398253220753895	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	331	0.13733585046573865	No Hit
TCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	284	0.11783498952347364	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	272	0.11285604630417194	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	259	0.10746219114992843	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	252	0.10455780760533577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23981909839636537	0.0
2	0.0	0.0	0.0	1.294110325083501	0.0
3	0.0	0.0	0.0	1.6434661743045038	0.0
4	0.0	0.0	0.0	2.174553451030019	0.0
5	0.0	0.0	0.0	4.1889508951725	0.0
6	0.0	0.0	0.0	5.0324668589091965	0.0
7	0.0	0.0	0.0	5.940294172561874	0.0
8	0.0	0.0	0.0	7.30452461465054	0.0
9	0.0	0.0	0.0	7.757193535672053	0.0
10	0.0	0.0	0.0	9.875318963549987	0.0
11	0.0	0.0	0.0	11.254071323361616	0.0
12	0.0	0.0	0.0	13.458498433707446	0.0
13	0.0	0.0	0.0	14.012820778789703	0.0
14	0.0	0.0	0.0	14.268821442648798	0.0
15	0.0	0.0	0.0	14.806547310333382	0.0
16	0.0	0.0	0.0	15.636371180217	0.0
17	0.0	0.0	0.0	16.581125656079497	0.0
18	0.0	0.0	0.0	17.713420326535694	0.0
19	0.0	0.0	0.0	18.496774059705828	0.0
20	0.0	0.0	0.0	19.104205132460635	0.0
21	0.0	0.0	0.0	19.755616870319276	0.0
22	0.0	0.0	0.0	20.430678588469597	0.0
23	0.0	0.0	0.0	21.091218388896955	0.0
24	0.0	0.0	0.0	21.58579341534759	0.0
25	0.0	0.0	0.0	22.044686015393232	0.0
26	0.0	0.0	0.0	22.469555836773644	0.0
27	4.149119349418086E-4	0.0	0.0	22.95002385743626	0.0
28	4.149119349418086E-4	0.0	0.0	23.387755948799867	0.0
29	4.149119349418086E-4	0.0	0.0	23.81718980146464	0.0
30	4.149119349418086E-4	0.0	0.0	24.3030516772815	0.0
31	4.149119349418086E-4	0.0	0.0	24.787253905358586	0.0
32	4.149119349418086E-4	0.0	0.0	25.239093002510216	0.0
33	4.149119349418086E-4	0.0	0.0	25.685123332572662	0.0
34	4.149119349418086E-4	0.0	0.0	26.11414227330249	0.0
35	4.149119349418086E-4	0.0	0.0	26.636516399394228	0.0
36	4.149119349418086E-4	0.0	0.0	27.09582391137481	0.0
37	4.149119349418086E-4	0.0	0.0	27.566748957533765	0.0
38	4.149119349418086E-4	0.0	0.0	27.992863514719	0.0
39	4.149119349418086E-4	0.0	0.0	28.434744725432026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAGGG	35	1.2065902E-7	45.000004	3
CATCGAA	35	1.2065902E-7	45.000004	42
ATCGAAT	35	1.2065902E-7	45.000004	43
AGCCGAT	20	7.0221827E-4	45.0	28
CGAAATT	20	7.0221827E-4	45.0	13
CACGGGC	55	1.8189894E-12	45.0	4
TTTCGTC	20	7.0221827E-4	45.0	16
GATCAAC	20	7.0221827E-4	45.0	9
TAACGCC	20	7.0221827E-4	45.0	12
TATGCGG	25	3.881604E-5	45.0	2
TATGCAG	40	6.7757355E-9	45.0	1
GCTTGCG	20	7.0221827E-4	45.0	1
AACGGGC	55	1.8189894E-12	45.0	4
AATGCGG	20	7.0221827E-4	45.0	2
TACGCGG	20	7.0221827E-4	45.0	2
TCGTGGA	20	7.0221827E-4	45.0	1
TACTAGA	20	7.0221827E-4	45.0	30
ATAACGC	20	7.0221827E-4	45.0	11
TAGGCCC	20	7.0221827E-4	45.0	31
CGGTACA	20	7.0221827E-4	45.0	36
>>END_MODULE