##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936428.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441813
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997752442775564	33.0	31.0	34.0	30.0	34.0
2	32.0934082971755	33.0	31.0	34.0	30.0	34.0
3	32.05128640397634	33.0	31.0	34.0	30.0	34.0
4	35.70450394171289	37.0	35.0	37.0	33.0	37.0
5	32.169578532093894	37.0	35.0	37.0	0.0	37.0
6	33.879888097453	37.0	35.0	37.0	17.0	37.0
7	35.491524694836954	37.0	35.0	37.0	32.0	37.0
8	35.72780791873485	37.0	35.0	37.0	35.0	37.0
9	37.644480809754356	39.0	37.0	39.0	35.0	39.0
10	37.24572386960094	39.0	37.0	39.0	34.0	39.0
11	37.230547765683674	39.0	37.0	39.0	34.0	39.0
12	37.05567513857673	39.0	37.0	39.0	34.0	39.0
13	36.931366890517026	39.0	37.0	39.0	33.0	39.0
14	37.89027484478727	40.0	37.0	41.0	33.0	41.0
15	38.097790241572795	40.0	37.0	41.0	33.0	41.0
16	38.137033088659685	40.0	37.0	41.0	33.0	41.0
17	38.161391810562385	40.0	37.0	41.0	34.0	41.0
18	38.122402464391044	40.0	37.0	41.0	34.0	41.0
19	37.97663038434813	40.0	36.0	41.0	34.0	41.0
20	38.056084814163455	40.0	36.0	41.0	34.0	41.0
21	37.96470905111438	40.0	36.0	41.0	33.0	41.0
22	38.08166577262326	40.0	36.0	41.0	34.0	41.0
23	38.10151353626987	40.0	36.0	41.0	34.0	41.0
24	38.05694037975343	40.0	36.0	41.0	34.0	41.0
25	37.78357812015491	40.0	35.0	41.0	33.0	41.0
26	37.84416031216827	40.0	36.0	41.0	34.0	41.0
27	37.81235952767347	40.0	36.0	41.0	34.0	41.0
28	37.69528058250889	40.0	35.0	41.0	33.0	41.0
29	37.689536070690544	40.0	36.0	41.0	33.0	41.0
30	37.510426356852335	40.0	35.0	41.0	33.0	41.0
31	37.41377460599846	40.0	35.0	41.0	33.0	41.0
32	37.362162272273565	39.0	35.0	41.0	33.0	41.0
33	37.317417097278714	39.0	35.0	41.0	33.0	41.0
34	37.214307863281526	39.0	35.0	41.0	32.0	41.0
35	37.218513262398346	39.0	35.0	41.0	32.0	41.0
36	37.086862541391945	39.0	35.0	41.0	32.0	41.0
37	37.001901256866596	39.0	35.0	41.0	32.0	41.0
38	36.83071118323816	39.0	35.0	41.0	31.0	41.0
39	36.7929576540301	39.0	35.0	41.0	31.0	41.0
40	36.707749658792295	39.0	35.0	41.0	31.0	41.0
41	36.572844167102375	39.0	35.0	41.0	31.0	41.0
42	36.581653323917585	39.0	35.0	41.0	31.0	41.0
43	36.51728898878032	39.0	35.0	41.0	31.0	41.0
44	36.44351343215342	39.0	35.0	41.0	31.0	41.0
45	36.40528911553078	39.0	35.0	41.0	31.0	41.0
46	36.30832275193351	38.0	35.0	40.0	31.0	41.0
47	36.20262871395817	38.0	35.0	40.0	31.0	41.0
48	36.171923415562695	38.0	35.0	40.0	31.0	41.0
49	36.159481500091665	38.0	35.0	40.0	31.0	41.0
50	36.02900774762173	38.0	35.0	40.0	31.0	41.0
51	34.98997087002872	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	21.0
10	14.0
11	13.0
12	14.0
13	13.0
14	15.0
15	18.0
16	41.0
17	71.0
18	124.0
19	205.0
20	352.0
21	611.0
22	962.0
23	1411.0
24	1887.0
25	2543.0
26	3126.0
27	3791.0
28	4476.0
29	5385.0
30	6737.0
31	8778.0
32	12010.0
33	18347.0
34	31445.0
35	37101.0
36	35331.0
37	54423.0
38	86427.0
39	126005.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.67427169413304	19.542883527646314	22.578104311099946	11.204740467120706
2	34.44986906225032	23.350150402998555	23.427332378178097	18.77264815657303
3	33.824491357203165	24.387240755704337	24.319112384651426	17.469155502441076
4	29.411085685572857	25.217229914013394	24.732183072929047	20.639501327484705
5	24.058594925907567	36.0313073630699	21.36356331751216	18.546534393510377
6	25.43474275315575	35.010739837895215	24.658396199296988	14.896121209652048
7	80.105383952034	5.703317919572308	9.558342556692537	4.6329555717011495
8	80.22760760774355	5.492821623628096	8.845371231720208	5.4341995369081495
9	74.82532202538178	6.112088145889777	11.216057472278996	7.8465323564494485
10	40.689839366428785	24.78446763676035	18.511904357726007	16.01378863908486
11	33.138907184713894	24.374792050030216	23.343133859800414	19.143166905455473
12	28.232985448594768	22.324603395554227	29.59442116913717	19.847989986713834
13	26.798668214832972	24.356458501673785	30.90244062533244	17.942432658160808
14	21.888898696960027	27.668719571402377	30.61612039482768	19.826261336809917
15	19.694984076973743	24.958296835991696	35.445765516179925	19.900953570854636
16	24.752327342110803	24.11495361159586	29.872140475721633	21.260578570571713
17	24.023059529710533	23.34109679887192	27.6904482213063	24.945395450111246
18	27.062128094917988	22.353574928759453	28.980134129145135	21.604162847177424
19	25.33130532600897	25.32428878281083	26.85502690052126	22.489378990658945
20	25.719931283144682	27.151532435668486	26.519364527526353	20.609171753660487
21	24.388146116117	27.012785952427837	28.9638376417172	19.635230289737965
22	23.31574670731735	22.564523904909997	28.971533205224837	25.14819618254782
23	21.597825324288785	27.053074490791353	28.274405687474115	23.07469449744575
24	22.873478145731337	25.97162147786507	28.37422167297024	22.780678703433352
25	22.871893765009176	26.428375806053694	26.788709250293675	23.911021178643455
26	21.841367275295205	25.10270182181149	27.78143694277896	25.27449396011435
27	24.307795379493133	23.825464619646773	28.139506985987282	23.72723301487281
28	19.86179673300695	25.47684201234459	29.875082897062782	24.786278357585676
29	23.636923313709644	25.58231650041986	27.20155359846813	23.579206587402364
30	25.9091516093913	23.58328070925935	28.260598941180998	22.246968740168352
31	25.477747372757253	24.93430478505612	26.518685507216855	23.06926233496977
32	26.067589681607377	25.61015633310926	25.289658747026454	23.03259523825691
33	24.163616733776507	23.93659761030119	25.827895512354775	26.07189014356753
34	21.615706192438882	25.82721649204528	29.274828943467035	23.28224837204881
35	22.892717054500434	26.379033663563543	26.386955567174347	24.341293714761676
36	25.805940522347694	25.9999139907608	26.990830962420752	21.20331452447076
37	22.609565585439995	26.281481079099077	27.47112466133862	23.63782867412231
38	21.422411744335275	27.78664276515177	26.011004655815924	24.77994083469703
39	22.27028177079443	23.79694576664788	26.711074594907803	27.221697867649887
40	23.99657773764013	24.42979269509951	27.605344342515952	23.968285224744406
41	21.251298626341914	25.74256529346126	26.71922283862177	26.286913241575053
42	23.17609486366404	24.361211643840267	29.431682634960943	23.03101085753475
43	23.195786452639467	22.48643656931779	29.550511189123004	24.767265788919744
44	22.914219364301186	23.165004198608912	28.511383775488724	25.409392661601178
45	22.893169734706763	22.771851439409886	26.35119383087415	27.983784995009202
46	24.12400721572249	24.22359686111545	26.981551018190952	24.670844904971105
47	20.871047253023338	25.52233637308092	30.275478539563117	23.331137834332623
48	21.240434301389953	26.163557885349686	28.21012509817502	24.38588271508534
49	23.648919339177436	22.886605871714956	29.92872550151308	23.535749287594527
50	21.365374038337485	24.03075509321817	28.619348004698818	25.984522863745525
51	21.664369314619535	23.190354290163487	26.42362266388721	28.72165373132977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	383.0
2	622.0
3	1077.0
4	1532.0
5	1081.5
6	631.0
7	654.0
8	677.0
9	711.0
10	745.0
11	777.5
12	810.0
13	793.0
14	776.0
15	815.5
16	855.0
17	847.0
18	839.0
19	815.5
20	792.0
21	877.0
22	962.0
23	1168.5
24	1375.0
25	1555.0
26	2097.0
27	2459.0
28	2947.0
29	3435.0
30	4232.5
31	5030.0
32	5671.0
33	6312.0
34	7031.0
35	7750.0
36	8382.0
37	9014.0
38	10864.5
39	12715.0
40	14278.5
41	15842.0
42	18342.5
43	20843.0
44	24461.0
45	28079.0
46	34785.0
47	41491.0
48	44440.0
49	47389.0
50	48508.0
51	49627.0
52	44377.5
53	39128.0
54	35931.5
55	32735.0
56	29434.0
57	26133.0
58	23723.0
59	21313.0
60	18801.0
61	16289.0
62	14715.5
63	13142.0
64	11511.0
65	9880.0
66	8589.5
67	7299.0
68	5842.5
69	4386.0
70	3905.0
71	3424.0
72	2780.0
73	2136.0
74	1813.5
75	1197.0
76	903.0
77	750.5
78	598.0
79	461.0
80	324.0
81	200.5
82	77.0
83	63.5
84	50.0
85	30.5
86	11.0
87	8.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.28813865126056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09472082758313	26.161832254152596
2	11.0078517608608	7.98908901901279
3	4.256876895462603	4.634224170116838
4	2.1923550386599744	3.182259344627315
5	1.4061195534497128	2.5512730657915887
6	0.9520101033204118	2.0728004776015196
7	0.7095973318798224	1.8024976456073687
8	0.6131715828865277	1.780068433343938
9	0.5186799950825065	1.693973841947023
>10	6.120514494414412	41.749501927941694
>50	0.08351775186868284	2.103946452095492
>100	0.03956104035884976	2.3951674599778743
>500	0.0018838590647071312	0.4979013319428842
>1k	0.003139765107845219	1.3854645758410689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1524	0.3449423172247082	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	1233	0.27907734720345484	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	1129	0.25553797647420967	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1129	0.25553797647420967	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	1065	0.24105220987159726	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	762	0.1724711586123541	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.1679443565490377	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	681	0.15413761025592276	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	448	0.10140036621828692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5268020633163804E-4	0.0	0.0	0.18152476273898685	0.0
2	4.5268020633163804E-4	0.0	0.0	1.0026866570245783	0.0
3	4.5268020633163804E-4	0.0	0.0	1.348081654455618	0.0
4	4.5268020633163804E-4	0.0	0.0	1.8627790490546905	0.0
5	4.5268020633163804E-4	0.0	0.0	3.3935171667651245	0.0
6	4.5268020633163804E-4	0.0	0.0	4.265831924366191	0.0
7	4.5268020633163804E-4	0.0	0.0	5.12343457526148	0.0
8	6.790203094974571E-4	0.0	0.0	6.544171402833325	0.0
9	6.790203094974571E-4	0.0	0.0	7.075391624963503	0.0
10	6.790203094974571E-4	0.0	0.0	8.449955071489521	0.0
11	6.790203094974571E-4	0.0	0.0	10.03297775303126	0.0
12	9.053604126632761E-4	0.0	0.0	11.530670215679484	0.0
13	9.053604126632761E-4	0.0	0.0	12.057589975849512	0.0
14	9.053604126632761E-4	0.0	0.0	12.287551520665984	0.0
15	9.053604126632761E-4	0.0	0.0	12.74023172699762	0.0
16	9.053604126632761E-4	0.0	0.0	13.541928372410952	0.0
17	9.053604126632761E-4	0.0	0.0	14.460642851161012	0.0
18	9.053604126632761E-4	0.0	0.0	15.549112407285435	0.0
19	9.053604126632761E-4	0.0	0.0	16.181053975324403	0.0
20	9.053604126632761E-4	0.0	0.0	16.75392077643709	0.0
21	9.053604126632761E-4	0.0	0.0	17.442447370267512	0.0
22	9.053604126632761E-4	0.0	0.0	18.176468324834264	0.0
23	9.053604126632761E-4	0.0	0.0	18.88423382743378	0.0
24	9.053604126632761E-4	0.0	0.0	19.464796192054106	0.0
25	9.053604126632761E-4	0.0	0.0	19.999637855834933	0.0
26	9.053604126632761E-4	0.0	0.0	20.4984914432124	0.0
27	9.053604126632761E-4	0.0	0.0	21.099198077014485	0.0
28	9.053604126632761E-4	0.0	0.0	21.59646728366979	0.0
29	9.053604126632761E-4	0.0	0.0	22.157338059314686	0.0
30	9.053604126632761E-4	0.0	0.0	22.857634338509733	0.0
31	9.053604126632761E-4	0.0	0.0	23.425295317249606	0.0
32	9.053604126632761E-4	0.0	0.0	23.965795483609583	0.0
33	9.053604126632761E-4	0.0	0.0	24.53006136080197	0.0
34	9.053604126632761E-4	0.0	0.0	25.116508568104607	0.0
35	9.053604126632761E-4	0.0	0.0	25.741886273151763	0.0
36	9.053604126632761E-4	0.0	0.0	26.31203699302646	0.0
37	0.0011317005158290952	0.0	0.0	26.852537159386436	0.0
38	0.0011317005158290952	0.0	0.0	27.464787138449978	0.0
39	0.0011317005158290952	0.0	0.0	28.42605355659521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	35	1.2096643E-7	45.0	2
AAGTACG	20	7.0285675E-4	45.0	1
TAATACG	50	2.1827873E-11	45.0	4
GCCGATG	40	6.7993824E-9	45.0	9
TACTACG	25	3.8868937E-5	45.0	1
AGTACGG	20	7.0285675E-4	45.0	2
TACACGG	45	3.8380676E-10	45.0	2
CGCACGG	90	0.0	42.5	2
GTTATGG	115	0.0	41.08696	2
ACGGGAA	185	0.0	40.135136	5
CCACGGG	45	1.9237632E-8	40.0	3
AACGGGA	220	0.0	39.886364	4
CACGGGA	170	0.0	39.705883	4
CGACAGG	40	3.451787E-7	39.375	2
GCGTATG	40	3.451787E-7	39.375	1
CGAGACA	250	0.0	38.699997	22
CGTATGG	70	0.0	38.571426	2
CGAAAAC	35	6.239561E-6	38.571426	19
TACGGGT	35	6.239561E-6	38.571426	4
TCTACGG	35	6.239561E-6	38.571426	2
>>END_MODULE