SRR2936427.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242848
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	5989	2.4661516668862826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	5641	2.3228521544340492	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	5053	2.080725392014758	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2244	0.9240347871919885	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	1202	0.4949598102516801	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGAGGTGT	953	0.3924265384108578	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	834	0.3434246936355251	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	831	0.3421893530109369	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTA	829	0.34136579259454475	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	774	0.3187178811437607	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	673	0.2771280801159573	No Hit
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	609	0.25077414679140864	TruSeq Adapter, Index 15 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	594	0.2445974436684675	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.196830939517723	No Hit
GAATATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	428	0.17624192910791936	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTT	321	0.13218144683093952	No Hit
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	264	0.10870997496376335	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	258	0.1062392937145869	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATG	247	0.10170971142443008	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	55	1.8189894E-12	45.000004	43
CAGGTTC	20	7.0222875E-4	45.0	42
AGCGTTT	20	7.0222875E-4	45.0	4
CAGCGTT	20	7.0222875E-4	45.0	3
ATAGCAG	25	3.88169E-5	45.0	1
TAAACGG	25	3.88169E-5	45.0	2
CTAAGCG	20	7.0222875E-4	45.0	1
CGCGAGG	25	3.88169E-5	45.0	2
CCGTCGA	40	6.7775545E-9	45.0	41
CGTTATA	20	7.0222875E-4	45.0	10
AATGCGG	20	7.0222875E-4	45.0	2
TCAGCGT	20	7.0222875E-4	45.0	2
TCTAAGG	25	3.88169E-5	45.0	2
AACGCGG	20	7.0222875E-4	45.0	18
TCCACGT	20	7.0222875E-4	45.0	18
GGTATAT	20	7.0222875E-4	45.0	8
CGAATGC	40	6.7775545E-9	45.0	45
CGGGAGC	25	3.88169E-5	45.0	6
AACGACA	25	3.88169E-5	45.0	15
GTTCCCG	20	7.0222875E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content