##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242848
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947522730267494	33.0	31.0	34.0	30.0	34.0
2	32.05659918961655	33.0	31.0	34.0	30.0	34.0
3	32.04322456845434	33.0	31.0	34.0	30.0	34.0
4	35.70516125312953	37.0	35.0	37.0	35.0	37.0
5	32.14310597575438	37.0	35.0	37.0	0.0	37.0
6	33.856618131506124	37.0	35.0	37.0	18.0	37.0
7	35.41055722097773	37.0	35.0	37.0	32.0	37.0
8	35.532625345895376	37.0	35.0	37.0	33.0	37.0
9	37.446966826986426	39.0	37.0	39.0	35.0	39.0
10	37.19970516537093	39.0	37.0	39.0	34.0	39.0
11	37.197749209382	39.0	37.0	39.0	34.0	39.0
12	37.047729443932006	39.0	37.0	39.0	33.0	39.0
13	36.78621195150876	39.0	35.0	39.0	33.0	39.0
14	37.968189978916854	40.0	37.0	41.0	33.0	41.0
15	37.98650596257742	40.0	37.0	41.0	33.0	41.0
16	38.15896774937409	40.0	37.0	41.0	34.0	41.0
17	38.034618362103046	40.0	36.0	41.0	33.0	41.0
18	38.01836951508763	40.0	36.0	41.0	34.0	41.0
19	37.90384932138622	40.0	36.0	41.0	34.0	41.0
20	37.76919307550402	40.0	36.0	41.0	33.0	41.0
21	37.93397104361576	40.0	36.0	41.0	34.0	41.0
22	37.94816510739228	40.0	36.0	41.0	33.0	41.0
23	37.87443174331269	40.0	36.0	41.0	33.0	41.0
24	37.86365957306628	40.0	36.0	41.0	33.0	41.0
25	37.778149295032286	40.0	36.0	41.0	33.0	41.0
26	37.73317466069311	40.0	36.0	41.0	33.0	41.0
27	37.616064369482146	40.0	36.0	41.0	33.0	41.0
28	37.58173837132692	40.0	36.0	41.0	33.0	41.0
29	37.56649838582158	40.0	36.0	41.0	33.0	41.0
30	37.46445513242851	40.0	36.0	41.0	33.0	41.0
31	37.47832388984056	40.0	35.0	41.0	33.0	41.0
32	37.16319673211227	40.0	35.0	41.0	32.0	41.0
33	37.17815258927395	40.0	35.0	41.0	32.0	41.0
34	36.96301801950191	39.0	35.0	41.0	32.0	41.0
35	36.77561684675188	39.0	35.0	41.0	31.0	41.0
36	36.77512271050204	39.0	35.0	41.0	31.0	41.0
37	36.59629480168665	39.0	35.0	41.0	31.0	41.0
38	36.5346060086968	39.0	35.0	41.0	31.0	41.0
39	36.542302180787985	39.0	35.0	41.0	31.0	41.0
40	36.32244860983002	39.0	35.0	41.0	30.0	41.0
41	36.30259668599288	39.0	35.0	41.0	30.0	41.0
42	36.118955066543684	39.0	35.0	41.0	30.0	41.0
43	36.19918220450652	39.0	35.0	41.0	30.0	41.0
44	36.310091085782055	39.0	35.0	41.0	31.0	41.0
45	36.3797025299776	39.0	35.0	41.0	31.0	41.0
46	36.32101149690341	39.0	35.0	40.0	31.0	41.0
47	36.04315868362103	38.0	35.0	40.0	30.0	41.0
48	36.08826508762683	38.0	35.0	40.0	30.0	41.0
49	36.09118049150086	38.0	35.0	40.0	30.0	41.0
50	35.972167775728025	38.0	35.0	40.0	30.0	41.0
51	34.924306562129395	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	10.0
10	8.0
11	1.0
12	4.0
13	2.0
14	4.0
15	11.0
16	14.0
17	25.0
18	50.0
19	135.0
20	378.0
21	854.0
22	1190.0
23	1407.0
24	1568.0
25	1705.0
26	1919.0
27	1986.0
28	2224.0
29	2583.0
30	3442.0
31	4708.0
32	6579.0
33	9808.0
34	15964.0
35	22599.0
36	18552.0
37	28840.0
38	46642.0
39	69587.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.268497166952166	16.912636711029123	19.863041902753984	10.955824219264725
2	28.95473711951509	33.867686783502435	21.167973382527343	16.00960271445513
3	28.23206285413098	33.81497891685334	23.323642772433786	14.629315456581896
4	25.971801291342732	21.66746277506918	35.03673079457109	17.324005139017
5	29.481404005797867	33.296135854526284	19.1906048227698	18.031855316906046
6	22.99092436421136	39.89203122941099	22.734797074713402	14.38224733166425
7	70.18299512452234	5.223020160758994	19.24413624983529	5.349848464883384
8	68.25421662933194	14.306479773356173	10.80964224535512	6.62966135195678
9	63.47468375280011	6.651485702991171	11.622908156542364	18.25092238766636
10	41.3274970351825	22.817976676769007	19.852747397549084	16.00177889049941
11	35.27803399657399	22.280191724864938	23.240874950586374	19.2008993279747
12	28.232474634339177	18.778824614573725	29.128096587165636	23.860604163921465
13	25.493312689418897	24.511628673079457	33.353373303465546	16.641685334036104
14	19.702859401765714	32.748056397417315	25.598728422717095	21.95035577809988
15	15.26098629595467	23.897664382659112	39.99991764395836	20.841431677427856
16	16.840575174594807	23.848250757675583	30.607211095005933	28.70396297272368
17	17.384948609830015	25.187359994729213	31.133466201080513	26.29422519436026
18	19.550500724733165	23.465295164053234	30.682155092897617	26.30204901831598
19	19.692564896560814	26.39922914745026	26.794126367110294	27.114079588878642
20	24.841464619844512	24.417742785610752	31.586836210304387	19.15395638424035
21	21.91164843852945	31.541952167611015	26.768595994202133	19.777803399657397
22	19.218605876927132	23.366056133877983	29.252042429832652	28.163295559362233
23	19.286549611279483	30.488206614837267	27.711160890762947	22.514082883120306
24	21.865529055211493	24.64257477928581	27.279203452365262	26.212692713137436
25	17.77366912636711	32.66487679536171	26.09904137567532	23.462412702595863
26	18.294571089735143	24.182616286730795	31.647367900909213	25.875444722624852
27	23.15234220582422	28.871969297667675	25.45378178943207	22.52190670707603
28	17.27829753590723	26.937837659770718	32.82547107655817	22.958393727763866
29	21.161384899196207	24.08214191593095	31.45877256555541	23.297700619317432
30	20.761134536829623	31.539069706153644	27.71692581367769	19.982869943339043
31	27.415090921069968	24.556924495981026	22.68661879035446	25.341365792594544
32	25.812854130979048	30.911104888654634	24.47168599288444	18.80435498748188
33	27.77169258136777	24.1924990117275	22.274015021741995	25.761793385162733
34	25.908798919488735	23.432764527605745	28.653725787323758	22.004710765581763
35	22.416902753986033	25.909210699696928	28.06117406772961	23.61271247858743
36	29.794356964026882	24.14637962840954	27.188200026353933	18.871063381209645
37	21.355333377256557	30.605563974173144	29.2158057715114	18.8232968770589
38	27.07701937014099	25.361543022796152	23.57606404005798	23.985373567004874
39	27.60986295954671	25.94997694030834	25.69096718935301	20.749192910791937
40	25.338895111345366	22.032711819739095	31.399476215575174	21.22891685334036
41	25.947506259059168	23.477648570299117	25.505254315456583	25.069590855185137
42	22.50090591645803	22.367489129002504	33.00624258795625	22.12536236658321
43	28.18347278956384	23.637419291079194	25.027177493740943	23.151930425616023
44	24.147203188825934	23.912076689945973	28.582076031097642	23.35864409013045
45	22.423079457108976	22.516965344577677	26.90654236394782	28.153412834365525
46	28.820084991434975	25.67284886019238	25.30883515614705	20.19823099222559
47	20.61165832125445	23.72389313480037	35.557632757939125	20.10681578600606
48	24.348975490842008	23.73665832125445	26.530587033864805	25.383779154038738
49	22.141833574911054	20.042166293319276	34.797074713400974	23.018925418368692
50	23.82436750560021	20.918846356568718	30.604740413756755	24.652045724074316
51	21.964768085386744	21.480102780339966	26.017920674660694	30.537208459612597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	253.5
2	405.0
3	684.0
4	963.0
5	656.5
6	350.0
7	343.5
8	337.0
9	334.5
10	332.0
11	341.5
12	351.0
13	389.0
14	427.0
15	403.0
16	379.0
17	369.5
18	360.0
19	415.0
20	470.0
21	516.0
22	562.0
23	585.5
24	609.0
25	837.5
26	1322.0
27	1578.0
28	1786.5
29	1995.0
30	2419.5
31	2844.0
32	3179.5
33	3515.0
34	4162.5
35	4810.0
36	5153.5
37	5497.0
38	6053.0
39	6609.0
40	7814.5
41	9020.0
42	10771.0
43	12522.0
44	15566.0
45	18610.0
46	30332.5
47	42055.0
48	34024.5
49	25994.0
50	25976.0
51	25958.0
52	22033.5
53	18109.0
54	16005.5
55	13902.0
56	12121.0
57	10340.0
58	9182.5
59	8025.0
60	7507.5
61	6990.0
62	6648.5
63	6307.0
64	5187.0
65	4067.0
66	3486.5
67	2906.0
68	2332.0
69	1758.0
70	1403.5
71	1049.0
72	893.5
73	738.0
74	558.0
75	361.0
76	344.0
77	239.5
78	135.0
79	94.0
80	53.0
81	35.0
82	17.0
83	11.0
84	5.0
85	3.0
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	242848.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.081203057056264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89839660069958	25.92444656740018
2	10.43031199380238	7.318157860060614
3	3.8559053454468626	4.058093951772302
4	2.025964269784257	2.84293055738569
5	1.2125267037584808	2.1268447753327187
6	0.8850388525013498	1.8628936618790355
7	0.6725825762377632	1.6516504150744498
8	0.5716365002230204	1.6042956911319013
9	0.4906448810949128	1.5491171432336275
>10	5.8419607014578485	36.58008301488997
>50	0.06925370331243984	1.6042956911319013
>100	0.0305185811207362	2.0778429305573853
>500	0.009390332652534217	2.5106239293714587
>1k	0.0023475831631335543	1.4189945974436684
>5k	0.0035213747447003308	6.8697292133350905
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	5989	2.4661516668862826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	5641	2.3228521544340492	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	5053	2.080725392014758	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2244	0.9240347871919885	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	1202	0.4949598102516801	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGAGGTGT	953	0.3924265384108578	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	834	0.3434246936355251	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	831	0.3421893530109369	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTA	829	0.34136579259454475	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	774	0.3187178811437607	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	673	0.2771280801159573	No Hit
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	609	0.25077414679140864	TruSeq Adapter, Index 15 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	594	0.2445974436684675	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.196830939517723	No Hit
GAATATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	428	0.17624192910791936	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTT	321	0.13218144683093952	No Hit
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	264	0.10870997496376335	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	258	0.1062392937145869	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATG	247	0.10170971142443008	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012353406245882198	0.0	0.0	0.2598333113717222	0.0
2	0.0012353406245882198	0.0	0.0	1.7998912900250363	0.0
3	0.0012353406245882198	0.0	0.0	2.2116714982211096	0.0
4	0.0012353406245882198	0.0	0.0	3.0006423771247857	0.0
5	0.0012353406245882198	0.0	0.0	6.471537752009487	0.0
6	0.0012353406245882198	0.0	0.0	7.415337989194887	0.0
7	0.0012353406245882198	0.0	0.0	8.769683093951773	0.0
8	0.0012353406245882198	0.0	0.0	10.391685334036104	0.0
9	0.0012353406245882198	0.0	0.0	10.885821583871392	0.0
10	0.0012353406245882198	0.0	0.0	14.515664119119778	0.0
11	0.0012353406245882198	0.0	0.0	15.964306891553564	0.0
12	0.0012353406245882198	0.0	0.0	19.919044011068653	0.0
13	0.0012353406245882198	0.0	0.0	20.582421926472527	0.0
14	0.0012353406245882198	0.0	0.0	20.883845038872053	0.0
15	0.0012353406245882198	0.0	0.0	21.68434576360522	0.0
16	0.0012353406245882198	0.0	0.0	22.645028989326658	0.0
17	0.0012353406245882198	0.0	0.0	23.6575965212808	0.0
18	0.0012353406245882198	0.0	0.0	24.751696534457768	0.0
19	0.0012353406245882198	0.0	0.0	25.84085518513638	0.0
20	0.0012353406245882198	0.0	0.0	26.503821320332058	0.0
21	0.0012353406245882198	0.0	0.0	27.17584662010805	0.0
22	0.0012353406245882198	0.0	0.0	27.953287653182237	0.0
23	0.0012353406245882198	0.0	0.0	28.65331400711556	0.0
24	0.0012353406245882198	0.0	0.0	29.209629068388455	0.0
25	0.0012353406245882198	0.0	0.0	29.70541243905653	0.0
26	0.0012353406245882198	0.0	0.0	30.196666227434445	0.0
27	0.0012353406245882198	0.0	0.0	30.756687310581103	0.0
28	0.0012353406245882198	0.0	0.0	31.1977039135591	0.0
29	0.0012353406245882198	0.0	0.0	31.693899064435367	0.0
30	0.0012353406245882198	0.0	0.0	32.24897878508367	0.0
31	0.0012353406245882198	0.0	0.0	32.73323230992226	0.0
32	0.0012353406245882198	0.0	0.0	33.23107458163131	0.0
33	0.0012353406245882198	0.0	0.0	33.682385689814204	0.0
34	0.0012353406245882198	0.0	0.0	34.13781460007906	0.0
35	0.0012353406245882198	0.0	0.0	34.641833574911054	0.0
36	0.0012353406245882198	0.0	0.0	35.0779088153907	0.0
37	0.0012353406245882198	0.0	0.0	35.54322045065226	0.0
38	0.0012353406245882198	0.0	0.0	35.99370799841876	0.0
39	0.0012353406245882198	0.0	0.0	36.44296020556068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	55	1.8189894E-12	45.000004	43
CAGGTTC	20	7.0222875E-4	45.0	42
AGCGTTT	20	7.0222875E-4	45.0	4
CAGCGTT	20	7.0222875E-4	45.0	3
ATAGCAG	25	3.88169E-5	45.0	1
TAAACGG	25	3.88169E-5	45.0	2
CTAAGCG	20	7.0222875E-4	45.0	1
CGCGAGG	25	3.88169E-5	45.0	2
CCGTCGA	40	6.7775545E-9	45.0	41
CGTTATA	20	7.0222875E-4	45.0	10
AATGCGG	20	7.0222875E-4	45.0	2
TCAGCGT	20	7.0222875E-4	45.0	2
TCTAAGG	25	3.88169E-5	45.0	2
AACGCGG	20	7.0222875E-4	45.0	18
TCCACGT	20	7.0222875E-4	45.0	18
GGTATAT	20	7.0222875E-4	45.0	8
CGAATGC	40	6.7775545E-9	45.0	45
CGGGAGC	25	3.88169E-5	45.0	6
AACGACA	25	3.88169E-5	45.0	15
GTTCCCG	20	7.0222875E-4	45.0	45
>>END_MODULE