##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272330
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971552895384278	33.0	31.0	34.0	30.0	34.0
2	32.073781808834866	33.0	31.0	34.0	30.0	34.0
3	32.03364667866192	33.0	31.0	34.0	30.0	34.0
4	35.684412293908125	37.0	35.0	37.0	33.0	37.0
5	32.11822054125509	37.0	35.0	37.0	0.0	37.0
6	33.87020526567033	37.0	35.0	37.0	17.0	37.0
7	35.504237505967026	37.0	35.0	37.0	32.0	37.0
8	35.73004810340396	37.0	35.0	37.0	35.0	37.0
9	37.63341901369662	39.0	37.0	39.0	35.0	39.0
10	37.30281276392612	39.0	37.0	39.0	34.0	39.0
11	37.23574707156758	39.0	37.0	39.0	34.0	39.0
12	37.167234605074725	39.0	37.0	39.0	34.0	39.0
13	37.07525061506261	39.0	37.0	39.0	33.0	39.0
14	38.27388829728638	40.0	38.0	41.0	33.0	41.0
15	38.41675540704293	40.0	38.0	41.0	34.0	41.0
16	38.40722652664047	40.0	37.0	41.0	34.0	41.0
17	38.36895678037675	40.0	37.0	41.0	34.0	41.0
18	38.325729813094405	40.0	37.0	41.0	34.0	41.0
19	38.194587449050786	40.0	37.0	41.0	34.0	41.0
20	38.25828223111666	40.0	37.0	41.0	34.0	41.0
21	38.2359600484706	40.0	37.0	41.0	34.0	41.0
22	38.318819079792895	40.0	37.0	41.0	34.0	41.0
23	38.33862225975838	40.0	37.0	41.0	34.0	41.0
24	38.319994124775086	40.0	37.0	41.0	34.0	41.0
25	38.078963022803215	40.0	37.0	41.0	34.0	41.0
26	38.115371057173284	40.0	37.0	41.0	34.0	41.0
27	38.05349025079866	40.0	37.0	41.0	34.0	41.0
28	37.9971248118092	40.0	37.0	41.0	34.0	41.0
29	37.99401461462197	40.0	36.0	41.0	34.0	41.0
30	37.8532221936621	40.0	36.0	41.0	34.0	41.0
31	37.857911357544154	40.0	36.0	41.0	34.0	41.0
32	37.784856607792015	40.0	36.0	41.0	33.0	41.0
33	37.722652664047295	40.0	36.0	41.0	33.0	41.0
34	37.63802004920501	40.0	36.0	41.0	33.0	41.0
35	37.63526970954357	40.0	36.0	41.0	33.0	41.0
36	37.526713913266995	40.0	36.0	41.0	33.0	41.0
37	37.365057099842105	40.0	36.0	41.0	33.0	41.0
38	37.31929276980134	40.0	35.0	41.0	33.0	41.0
39	37.27474387691404	40.0	35.0	41.0	32.0	41.0
40	37.17171079205376	39.0	35.0	41.0	32.0	41.0
41	37.138985789299745	39.0	35.0	41.0	32.0	41.0
42	37.1100980428157	39.0	35.0	41.0	32.0	41.0
43	37.00860353247898	39.0	35.0	41.0	32.0	41.0
44	36.93776668013072	39.0	35.0	41.0	32.0	41.0
45	36.976656997025664	39.0	35.0	41.0	32.0	41.0
46	36.909749201336616	39.0	35.0	41.0	32.0	41.0
47	36.81017515514266	39.0	35.0	41.0	31.0	41.0
48	36.82505049021408	39.0	35.0	41.0	31.0	41.0
49	36.797613189879925	39.0	35.0	41.0	31.0	41.0
50	36.65304960893034	39.0	35.0	41.0	31.0	41.0
51	35.68393493188411	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	6.0
10	8.0
11	9.0
12	6.0
13	10.0
14	5.0
15	7.0
16	13.0
17	22.0
18	46.0
19	90.0
20	214.0
21	354.0
22	561.0
23	758.0
24	1021.0
25	1247.0
26	1658.0
27	2049.0
28	2379.0
29	2914.0
30	3508.0
31	4819.0
32	6580.0
33	9579.0
34	16124.0
35	22465.0
36	19595.0
37	31267.0
38	54055.0
39	90882.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.927955054529434	20.341864649506114	23.824404215473873	11.90577608049058
2	31.838945397128484	26.04450482870047	24.163698454081445	17.952851320089596
3	31.193405060037456	26.465685014504466	25.970330114199687	16.370579811258402
4	28.383578746373882	25.312304924172878	27.176587228729847	19.127529100723386
5	23.803106525171668	36.06984173612896	21.842250211140897	18.28480152755848
6	24.18242573348511	36.01733191348731	24.183527338155912	15.616715014871662
7	78.89252010428524	5.183049976131899	11.018249917379649	4.906180002203209
8	78.75812433444717	6.7095068483090365	8.789703668343554	5.742665148900231
9	72.48925935445966	7.417838651635883	11.003929056659201	9.088972937245254
10	37.16593838357875	32.00235008996438	17.259941982154004	13.571769544302867
11	28.165093819997793	25.131641758161056	28.397165203980464	18.306099217860684
12	26.6019167921272	21.644328571953146	31.561341020085926	20.192413615833733
13	23.59270003304814	26.063599309661072	32.491829765358204	17.85187089193258
14	18.953108361179453	30.594866522234053	28.8899496933867	21.562075423199794
15	15.958579664377778	27.343663937135094	37.16997760070503	19.5277787977821
16	18.595454044725148	25.38170601843352	33.668710755333606	22.35412918150773
17	18.767671574927476	25.20875408511732	30.23390739176734	25.78966694818786
18	20.58972570043697	24.306539859729003	31.925604964565053	23.178129475268975
19	20.803804208129844	27.285646091139427	29.138545147431426	22.772004553299304
20	23.840561083978994	26.149157272426837	30.123746924686962	19.88653471890721
21	21.695369588367054	28.42323651452282	29.384937392134542	20.49645650497558
22	18.999742958910147	25.136415378401207	29.15286600815187	26.710975654536774
23	18.867183196856754	29.03866632394521	28.872691220210772	23.22145925898726
24	21.310909558256526	24.62049719090809	30.352513494657217	23.716079756178164
25	18.591782029155805	28.73352183013256	28.80806374619028	23.866632394521353
26	19.933903719751772	27.353211177615393	29.90195718429846	22.81092791833437
27	21.69500238681012	27.034480226196163	29.448830463041165	21.82168692395256
28	17.62273712040539	27.342562332464286	31.412991591084342	23.62170895604597
29	20.73513751698307	25.55539235486358	30.592663312892448	23.116806815260897
30	21.167700951052034	26.940843829177837	30.504534939228144	21.38692028054199
31	21.685822347886756	27.82212756582088	25.528586641207358	24.963463445085008
32	23.95108875261631	30.457533139940512	25.106672052289504	20.484706055153673
33	21.759997062387544	28.522380934895164	26.515991627804503	23.201630374912792
34	21.308339147357984	27.766680130723753	27.41526824073734	23.50971248118092
35	22.713252304189773	25.83116072412147	27.862519737083684	23.593067234605076
36	24.398340248962654	27.153086329086033	26.957000697682957	21.49157272426835
37	20.514449381265376	28.636947820658758	29.557522123893804	21.29108067418206
38	20.53868468402306	29.545771674071897	25.34682187052473	24.56872177138031
39	22.629163147651745	28.30499761318988	26.82003451694635	22.24580472221202
40	24.051701979216393	26.608893621708958	28.502552050820697	20.836852348253956
41	21.647633385965555	25.914882679102558	27.459699629126426	24.977784305805457
42	24.58230822898689	24.294422208350163	28.20915800682995	22.914111555832996
43	24.568354569823374	24.59736349282121	27.494950978592147	23.339330958763266
44	21.086182205412552	26.627253699555688	27.92604560643337	24.360518488598395
45	21.005765064443874	26.090772224874232	26.808651268681377	26.094811442000516
46	23.901149340873204	27.170344802261965	26.73851577130687	22.189990085557962
47	20.698784562846548	26.0140270994749	30.770756068005728	22.516432269672823
48	21.9792163918775	24.6238020049205	28.641354239341975	24.75562736386002
49	22.33907391767341	23.104689163882057	31.110050306613303	23.446186611831234
50	20.830977123343004	24.57716740718981	30.10465244372636	24.48720302574083
51	20.171850328645395	23.98927771453751	26.600815187456394	29.238056769360703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	235.0
2	388.0
3	704.0
4	1020.0
5	705.0
6	390.0
7	397.0
8	404.0
9	408.5
10	413.0
11	385.0
12	357.0
13	396.5
14	436.0
15	385.5
16	335.0
17	399.0
18	463.0
19	483.5
20	504.0
21	580.5
22	657.0
23	885.0
24	1113.0
25	1485.0
26	2178.0
27	2499.0
28	2935.5
29	3372.0
30	3964.0
31	4556.0
32	5164.0
33	5772.0
34	6569.0
35	7366.0
36	8121.0
37	8876.0
38	9326.0
39	9776.0
40	11621.0
41	13466.0
42	15651.5
43	17837.0
44	19724.0
45	21611.0
46	27234.5
47	32858.0
48	32141.0
49	31424.0
50	30599.0
51	29774.0
52	24725.0
53	19676.0
54	16794.0
55	13912.0
56	12263.5
57	10615.0
58	9754.0
59	8893.0
60	8076.5
61	7260.0
62	6588.5
63	5917.0
64	4774.5
65	3632.0
66	2743.5
67	1855.0
68	1511.0
69	1167.0
70	933.5
71	700.0
72	584.0
73	468.0
74	407.5
75	264.5
76	182.0
77	129.5
78	77.0
79	47.0
80	17.0
81	10.5
82	4.0
83	3.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	272330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.35842102362174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65182715667004	26.767991260306534
2	11.337011638100467	8.470657078517137
3	4.173183785546677	4.677106706082131
4	2.106196098625613	3.1473664244306074
5	1.368462849817679	2.5561805649337055
6	1.0365924617607196	2.323527456982169
7	0.8446678862283742	2.2088820963196563
8	0.6877295911035838	2.0553993291880728
9	0.687729591017973	2.3123242450487367
>10	5.986646105487129	37.985676660256665
>50	0.08096816405817904	2.0692165119051134
>100	0.03198742283779912	2.1595883573988934
>500	0.0029988208910436682	0.8536778462759589
>1k	0.00399842785472489	2.4124054623546347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	1754	0.6440715308632908	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	1648	0.6051481658282231	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	1565	0.5746704366026512	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	1493	0.5482319245033599	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	999	0.3668343553776668	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	749	0.27503396614401643	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.19755443763081557	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	416	0.1527558476847942	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	333	0.12227811845922226	No Hit
ACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	293	0.1075900561818382	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.672015569346014E-4	0.0	0.0	0.2889876253075313	0.0
2	3.672015569346014E-4	0.0	0.0	1.5169096316968385	0.0
3	3.672015569346014E-4	0.0	0.0	1.991701244813278	0.0
4	3.672015569346014E-4	0.0	0.0	2.6493592332831493	0.0
5	3.672015569346014E-4	0.0	0.0	4.762971394998715	0.0
6	3.672015569346014E-4	0.0	0.0	5.76726765321485	0.0
7	3.672015569346014E-4	0.0	0.0	6.842066610362428	0.0
8	3.672015569346014E-4	0.0	0.0	8.589578819814196	0.0
9	3.672015569346014E-4	0.0	0.0	9.20757904013513	0.0
10	3.672015569346014E-4	0.0	0.0	11.196709874049866	0.0
11	3.672015569346014E-4	0.0	0.0	13.06760180663166	0.0
12	3.672015569346014E-4	0.0	0.0	15.1830499761319	0.0
13	3.672015569346014E-4	0.0	0.0	15.832262328792273	0.0
14	3.672015569346014E-4	0.0	0.0	16.080490581280063	0.0
15	3.672015569346014E-4	0.0	0.0	16.621378474644732	0.0
16	3.672015569346014E-4	0.0	0.0	17.5581096463849	0.0
17	3.672015569346014E-4	0.0	0.0	18.739397055043515	0.0
18	3.672015569346014E-4	0.0	0.0	20.024969705871552	0.0
19	3.672015569346014E-4	0.0	0.0	20.83575074358315	0.0
20	3.672015569346014E-4	0.0	0.0	21.510667205228952	0.0
21	3.672015569346014E-4	0.0	0.0	22.329526677193112	0.0
22	3.672015569346014E-4	0.0	0.0	23.19134873131862	0.0
23	3.672015569346014E-4	0.0	0.0	24.071898064847794	0.0
24	3.672015569346014E-4	0.0	0.0	24.69724231630742	0.0
25	3.672015569346014E-4	0.0	0.0	25.287335218301326	0.0
26	3.672015569346014E-4	0.0	0.0	25.819410274299564	0.0
27	3.672015569346014E-4	0.0	0.0	26.35882936143649	0.0
28	3.672015569346014E-4	0.0	0.0	26.88062277384056	0.0
29	3.672015569346014E-4	0.0	0.0	27.438769140381154	0.0
30	3.672015569346014E-4	0.0	0.0	28.108544780229867	0.0
31	3.672015569346014E-4	0.0	0.0	28.682113612161714	0.0
32	3.672015569346014E-4	0.0	0.0	29.22593911798186	0.0
33	7.344031138692028E-4	0.0	0.0	29.75654536775236	0.0
34	7.344031138692028E-4	0.0	0.0	30.280541989498037	0.0
35	7.344031138692028E-4	0.0	0.0	30.8978078067051	0.0
36	7.344031138692028E-4	0.0	0.0	31.43722689384203	0.0
37	0.0011016046708038042	0.0	0.0	32.0078581133184	0.0
38	0.0011016046708038042	0.0	0.0	32.50761943230639	0.0
39	0.0011016046708038042	0.0	0.0	33.05732016303749	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	65	0.0	45.000004	4
GATCGTA	20	7.0237945E-4	45.000004	9
CAAAACG	30	2.1593805E-6	45.000004	9
GCGAACG	20	7.0237945E-4	45.000004	1
GTCCTCG	20	7.0237945E-4	45.000004	23
ATTACGG	20	7.0237945E-4	45.000004	2
CCTCGTT	20	7.0237945E-4	45.000004	25
TATGCGT	20	7.0237945E-4	45.000004	20
TCGATAT	20	7.0237945E-4	45.000004	16
ACGGGTA	20	7.0237945E-4	45.000004	5
ATCGTCT	20	7.0237945E-4	45.000004	40
GTTACGT	20	7.0237945E-4	45.000004	25
GCTTACG	35	1.2073724E-7	45.000004	1
TGTTACG	20	7.0237945E-4	45.000004	1
GAGCCCG	35	1.2073724E-7	45.000004	3
ACGTCCC	20	7.0237945E-4	45.000004	39
TTACGTG	20	7.0237945E-4	45.000004	26
AACCTCG	20	7.0237945E-4	45.000004	26
ACATCGT	20	7.0237945E-4	45.000004	38
CCACGAG	20	7.0237945E-4	45.000004	40
>>END_MODULE