FastQCFastQC Report
Sat 14 Jan 2017
SRR2936424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790380
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147301.863660517725651No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC85641.0835294415344519No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG39620.5012778663427718No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC36670.4639540474202283No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT32740.41423112933019557No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT28910.36577342544092717No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC28880.3653938611806979No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG28250.3574230117158835No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC26950.340975227105949No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT19070.24127634808573092No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.21749032111136415No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT13780.17434651686530528No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC13570.1716895670437005No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT12360.1563804752144538No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT9790.12386447025481413Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC8940.11311014954831854No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC8220.10400060730281636No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT7980.1009640932209823No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC7930.10033148612060021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT302.1646374E-645.0000049
CAATGCG207.031947E-445.0000041
CGTTATT8550.043.947371
CGTTTTT67850.043.773031
TCACGAC750.039.025
CGACGGT750.039.028
CGGTCTA750.039.031
GGCGATA1450.038.7931068
TACGGGA3200.038.671884
TAGACGG700.038.571432
CGTTTCT4050.038.3333361
TATCACG301.1395991E-437.5000041
TCGTTAA301.1395991E-437.50000435
CCGATAT301.1395991E-437.5000049
TTCGAAT301.1395991E-437.50000417
GTTATTT10200.037.52
CGTTCTG3750.037.1999971
GTTTTTT81000.037.138892
CTCACGA850.037.05882324
CGTATGG850.037.0588232
Produced by FastQC (version 0.11.5)