##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	790380
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969265416635036	33.0	31.0	34.0	30.0	34.0
2	32.119423568410134	33.0	31.0	34.0	30.0	34.0
3	31.963701004580077	33.0	31.0	34.0	30.0	34.0
4	35.68143045117539	37.0	35.0	37.0	33.0	37.0
5	32.393544877147704	37.0	35.0	37.0	19.0	37.0
6	33.99909031098965	37.0	35.0	37.0	19.0	37.0
7	35.50160555682077	37.0	35.0	37.0	32.0	37.0
8	35.696144892330274	37.0	35.0	37.0	33.0	37.0
9	37.62425921708545	39.0	37.0	39.0	35.0	39.0
10	37.31593157721602	39.0	37.0	39.0	34.0	39.0
11	37.29394721526354	39.0	37.0	39.0	34.0	39.0
12	37.265159796553554	39.0	37.0	39.0	34.0	39.0
13	37.22720210531643	39.0	37.0	39.0	34.0	39.0
14	38.42638730737114	40.0	38.0	41.0	34.0	41.0
15	38.5202004099294	40.0	38.0	41.0	34.0	41.0
16	38.487586983476305	40.0	38.0	41.0	34.0	41.0
17	38.43862572433513	40.0	38.0	41.0	34.0	41.0
18	38.25973582327488	40.0	38.0	41.0	34.0	41.0
19	38.01901743465169	40.0	37.0	41.0	34.0	41.0
20	37.92892532705787	40.0	36.0	41.0	34.0	41.0
21	37.883123307776	40.0	35.0	41.0	34.0	41.0
22	37.94546547230446	40.0	35.0	41.0	34.0	41.0
23	37.94529340317316	40.0	35.0	41.0	34.0	41.0
24	37.90901971203725	40.0	35.0	41.0	34.0	41.0
25	37.65647030542271	40.0	35.0	41.0	33.0	41.0
26	37.65470153597004	40.0	35.0	41.0	33.0	41.0
27	37.636445760267215	40.0	35.0	41.0	33.0	41.0
28	37.53502492471976	40.0	35.0	41.0	33.0	41.0
29	37.44962802702498	40.0	35.0	41.0	33.0	41.0
30	37.22222348743642	39.0	35.0	41.0	33.0	41.0
31	37.10648169235051	39.0	35.0	41.0	32.0	41.0
32	36.85184215187631	39.0	35.0	41.0	31.0	41.0
33	36.57786128191503	39.0	35.0	41.0	31.0	41.0
34	36.283621802171105	39.0	35.0	41.0	30.0	41.0
35	36.133914066651485	39.0	35.0	41.0	29.0	41.0
36	35.95924112452238	39.0	35.0	41.0	27.0	41.0
37	35.83710873250841	39.0	35.0	41.0	27.0	41.0
38	35.66959943318404	39.0	35.0	41.0	26.0	41.0
39	35.566028998709484	39.0	35.0	41.0	25.0	41.0
40	35.43395328829171	39.0	35.0	41.0	24.0	41.0
41	35.31668311445128	39.0	35.0	41.0	24.0	41.0
42	35.26004833118247	39.0	35.0	41.0	23.0	41.0
43	35.204545914623345	39.0	35.0	41.0	23.0	41.0
44	35.14903970242162	38.0	35.0	41.0	23.0	41.0
45	35.13450618689744	38.0	35.0	41.0	23.0	41.0
46	35.020798856246365	38.0	35.0	40.0	23.0	41.0
47	34.90592373288798	38.0	34.0	40.0	23.0	41.0
48	34.86815582378097	38.0	34.0	40.0	23.0	41.0
49	34.85283028416711	38.0	34.0	40.0	23.0	41.0
50	34.74425845795693	38.0	34.0	40.0	23.0	41.0
51	33.75409423315367	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	19.0
9	48.0
10	37.0
11	30.0
12	26.0
13	16.0
14	24.0
15	49.0
16	70.0
17	142.0
18	315.0
19	582.0
20	1049.0
21	1881.0
22	2714.0
23	4089.0
24	6318.0
25	9936.0
26	13350.0
27	14355.0
28	13977.0
29	13345.0
30	13805.0
31	16173.0
32	20429.0
33	28469.0
34	46533.0
35	57354.0
36	61987.0
37	89758.0
38	144733.0
39	228598.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.539689769477974	16.616437662896328	19.158632556491813	20.685240011133885
2	39.185202054707865	24.10751790278094	20.58655330347428	16.120726739036918
3	29.058680634631447	24.65307826615046	31.215238239834004	15.073002859384093
4	24.921936283812848	22.455527720843136	35.02834079809712	17.594195197246894
5	22.30319593107113	32.856980186745616	27.827500695867812	17.01232318631544
6	22.197677066727397	32.344315392595966	30.87767909107012	14.580328449606519
7	71.49953187074571	4.647890887927326	19.125736987271946	4.726840254055011
8	71.83291581264709	5.916647688453655	16.96968546774969	5.280751031149573
9	66.38047521445381	5.497735266580632	18.695690680432197	9.426098838533365
10	34.94762013208836	24.034894607657076	25.239505048204663	15.777980212049899
11	26.565069966345302	22.596978668488575	31.7652268529062	19.072724512259924
12	22.662769806928313	20.221918570814037	36.209924340190796	20.905387282066854
13	22.512841924137756	21.167792707305345	38.670133353576766	17.649232014980136
14	19.287178319289456	25.321617449834257	36.042409980009616	19.34879425086667
15	18.145449024519852	23.40557706419697	38.93772615703839	19.511247754244792
16	21.12743237430097	23.479212530681444	34.712163769326146	20.68119132569144
17	20.595789367139858	24.07955666894405	33.482755130443586	21.841898833472506
18	22.26612515498874	22.63177205900959	33.50881854297933	21.593284243022342
19	20.455224069434955	25.738632050406135	31.865178774766566	21.940965105392344
20	21.98018674561603	25.281004073989727	32.60444343227309	20.13436574812116
21	21.505857941749536	26.640603254130923	33.34497330398036	18.508565500139174
22	20.46926794706344	21.811660214074244	34.180014676484724	23.539057162377592
23	19.598800576937673	25.926263316379462	33.82322427186923	20.651711834813636
24	21.125914117260052	23.965181305194967	33.131278625471296	21.777625952073688
25	21.689946607960728	26.334547938966068	30.938029808446572	21.037475644626635
26	20.10918798552595	24.223411523570938	32.3602570915256	23.307143399377512
27	22.14871327715782	23.410637921000024	31.823173663301198	22.617475138540954
28	19.135985222298135	25.37096080366406	33.14064121055695	22.35241276348086
29	20.323388749715328	25.887041676155775	31.515473569675343	22.274096004453554
30	20.576431589868164	26.025582631139454	33.13633948227435	20.261646296718034
31	21.490042764239988	24.231002808775525	31.823173663301198	22.455780763683293
32	21.776487259293	24.546167666185887	31.068599913965432	22.60874516055568
33	20.976618841569877	23.723272350008855	30.937650244186344	24.362458564234927
34	20.134492269541234	24.459880057693766	32.25587691996255	23.14975075280245
35	22.143019813254384	25.04618031832789	29.690655127913157	23.120144740504568
36	23.88420759634606	26.125028467319517	29.291859611832283	20.698904324502138
37	22.0973455806068	25.062375060097676	29.75277714517068	23.087502214124854
38	21.91983602823958	24.629292239176092	30.845289607530557	22.605582125053772
39	23.440117411877832	22.871783192894558	28.834231635415875	24.853867759811738
40	24.45709658645209	22.38518181128065	31.860624003643817	21.297097598623445
41	21.944001619474175	24.569068043219715	29.677623421645283	23.809306915660823
42	23.040436245856423	24.83008173283737	30.37096080366406	21.758521217642148
43	23.45567954654723	23.49237075836939	29.951162731850502	23.100786963232874
44	22.947443002100258	23.273741744477338	30.115640577949847	23.663174675472558
45	21.876312659733294	22.274728611553936	29.78213011462841	26.066828614084365
46	23.857132012449707	23.24691920342114	29.407753232622287	23.48819555150687
47	20.896530782661504	24.010855537842556	32.744882208557904	22.34773147093803
48	21.236746881246997	24.324502138211997	30.70624256686657	23.732508413674434
49	21.63895847566993	22.99261114906754	32.09759862344695	23.27083175181558
50	20.407905058326374	22.749563501100738	33.321566841266225	23.520964599306662
51	20.612490194589945	21.681090108555377	31.372377843568916	26.33404185328576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	747.0
2	1191.0
3	12520.0
4	23849.0
5	15104.0
6	6359.0
7	6083.5
8	5808.0
9	5797.0
10	5786.0
11	5745.0
12	5704.0
13	5490.0
14	5276.0
15	5158.0
16	5040.0
17	4863.5
18	4687.0
19	4302.5
20	3918.0
21	3848.0
22	3778.0
23	3776.5
24	3775.0
25	4180.0
26	5319.5
27	6054.0
28	7153.5
29	8253.0
30	9024.0
31	9795.0
32	11098.0
33	12401.0
34	14392.5
35	16384.0
36	17664.5
37	18945.0
38	20949.0
39	22953.0
40	25389.0
41	27825.0
42	30615.0
43	33405.0
44	39367.0
45	45329.0
46	64953.5
47	84578.0
48	79804.5
49	75031.0
50	74784.0
51	74537.0
52	65549.5
53	56562.0
54	50782.5
55	45003.0
56	41540.5
57	38078.0
58	35580.0
59	33082.0
60	30151.5
61	27221.0
62	24483.5
63	21746.0
64	19560.5
65	17375.0
66	14705.0
67	12035.0
68	9986.0
69	7937.0
70	6793.5
71	5650.0
72	4996.0
73	4342.0
74	3513.0
75	2146.5
76	1609.0
77	1193.5
78	778.0
79	598.0
80	418.0
81	310.5
82	203.0
83	131.0
84	59.0
85	38.5
86	18.0
87	19.5
88	21.0
89	13.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	790380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.54414804579568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41209313738045	29.030186793763136
2	9.236502198957313	7.304992207617706
3	4.030408789530577	4.7813724557481985
4	2.4706024562897326	3.9079147717531058
5	1.7321370283915898	3.424794154316081
6	1.467726967135565	3.4824007487529283
7	1.140657283951934	3.1574424354277335
8	0.9917377170060175	3.13739384831083
9	0.8565442429488759	3.0484181115849895
>10	4.579353990163682	27.59739145854506
>50	0.04482680094782032	1.214447883107546
>100	0.029669514062798	2.206103217671902
>500	0.00322494718073547	0.9363090250382909
>1k	0.0038699366168825637	3.800200577106515
>5k	3.22494718073547E-4	1.092147982896748
>10k+	3.22494718073547E-4	1.8784843283593062
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14730	1.863660517725651	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	8564	1.0835294415344519	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	3962	0.5012778663427718	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	3667	0.4639540474202283	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT	3274	0.41423112933019557	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	2891	0.36577342544092717	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	2888	0.3653938611806979	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	2825	0.3574230117158835	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	2695	0.340975227105949	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	1907	0.24127634808573092	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1719	0.21749032111136415	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1378	0.17434651686530528	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1357	0.1716895670437005	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT	1236	0.1563804752144538	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	979	0.12386447025481413	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	894	0.11311014954831854	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	822	0.10400060730281636	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	798	0.1009640932209823	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	793	0.10033148612060021	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2652142007641893E-4	0.0	0.0	0.12879880563779447	0.0
2	1.2652142007641893E-4	0.0	0.0	0.7416685644879678	0.0
3	1.2652142007641893E-4	0.0	0.0	1.3064601837091019	0.0
4	1.2652142007641893E-4	0.0	0.0	2.118601179179635	0.0
5	1.2652142007641893E-4	0.0	0.0	4.291733090412206	0.0
6	1.2652142007641893E-4	0.0	0.0	6.072901642248032	0.0
7	1.2652142007641893E-4	0.0	0.0	7.245755206356436	0.0
8	1.2652142007641893E-4	0.0	0.0	8.779068296262558	0.0
9	1.2652142007641893E-4	0.0	0.0	9.60892229054379	0.0
10	2.5304284015283785E-4	0.0	0.0	11.231812545864015	0.0
11	2.5304284015283785E-4	0.0	0.0	12.267263847769428	0.0
12	2.5304284015283785E-4	0.0	0.0	13.625218249449631	0.0
13	2.5304284015283785E-4	0.0	0.0	14.155089956729674	0.0
14	2.5304284015283785E-4	0.0	0.0	14.470507856980186	0.0
15	2.5304284015283785E-4	0.0	0.0	14.803385713201244	0.0
16	2.5304284015283785E-4	0.0	0.0	15.216731192590906	0.0
17	2.5304284015283785E-4	0.0	0.0	15.68612566107442	0.0
18	2.5304284015283785E-4	0.0	0.0	16.238012095447758	0.0
19	2.5304284015283785E-4	0.0	0.0	16.643639768212758	0.0
20	2.5304284015283785E-4	0.0	0.0	17.000683215668413	0.0
21	2.5304284015283785E-4	0.0	0.0	17.37392140489385	0.0
22	2.5304284015283785E-4	0.0	0.0	17.80915508995673	0.0
23	2.5304284015283785E-4	0.0	0.0	18.21857840532402	0.0
24	2.5304284015283785E-4	0.0	0.0	18.57258533869784	0.0
25	2.5304284015283785E-4	0.0	0.0	18.88876236746881	0.0
26	2.5304284015283785E-4	0.0	0.0	19.19620941825451	0.0
27	2.5304284015283785E-4	0.0	0.0	19.556036336951845	0.0
28	2.5304284015283785E-4	0.0	0.0	19.89688504263772	0.0
29	2.5304284015283785E-4	0.0	0.0	20.241529390925884	0.0
30	2.5304284015283785E-4	0.0	0.0	20.718009058933678	0.0
31	2.5304284015283785E-4	0.0	0.0	21.073154685088184	0.0
32	3.795642602292568E-4	0.0	0.0	21.463852830284168	0.0
33	3.795642602292568E-4	0.0	0.0	21.84278448341304	0.0
34	5.060856803056757E-4	0.0	0.0	22.20286444495053	0.0
35	6.326071003820947E-4	0.0	0.0	22.605708646473847	0.0
36	6.326071003820947E-4	0.0	0.0	22.95996862268782	0.0
37	7.591285204585136E-4	0.0	0.0	23.331182469192033	0.0
38	8.856499405349326E-4	0.0	0.0	23.72808016397176	0.0
39	0.0010121713606113514	0.0	0.0	24.26238012095448	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	30	2.1646374E-6	45.000004	9
CAATGCG	20	7.031947E-4	45.000004	1
CGTTATT	855	0.0	43.94737	1
CGTTTTT	6785	0.0	43.77303	1
TCACGAC	75	0.0	39.0	25
CGACGGT	75	0.0	39.0	28
CGGTCTA	75	0.0	39.0	31
GGCGATA	145	0.0	38.793106	8
TACGGGA	320	0.0	38.67188	4
TAGACGG	70	0.0	38.57143	2
CGTTTCT	405	0.0	38.333336	1
TATCACG	30	1.1395991E-4	37.500004	1
TCGTTAA	30	1.1395991E-4	37.500004	35
CCGATAT	30	1.1395991E-4	37.500004	9
TTCGAAT	30	1.1395991E-4	37.500004	17
GTTATTT	1020	0.0	37.5	2
CGTTCTG	375	0.0	37.199997	1
GTTTTTT	8100	0.0	37.13889	2
CTCACGA	85	0.0	37.058823	24
CGTATGG	85	0.0	37.058823	2
>>END_MODULE