##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936422.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527739
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.967205379932125	33.0	31.0	34.0	30.0	34.0
2	32.06824775125583	33.0	31.0	34.0	30.0	34.0
3	32.04248122651538	33.0	31.0	34.0	30.0	34.0
4	35.68703468949613	37.0	35.0	37.0	33.0	37.0
5	32.18384466563965	37.0	35.0	37.0	0.0	37.0
6	33.88128222473609	37.0	35.0	37.0	18.0	37.0
7	35.45981441583813	37.0	35.0	37.0	32.0	37.0
8	35.67353748728065	37.0	35.0	37.0	33.0	37.0
9	37.582983254980206	39.0	37.0	39.0	35.0	39.0
10	37.26942674314387	39.0	37.0	39.0	34.0	39.0
11	37.20609240552621	39.0	37.0	39.0	34.0	39.0
12	37.00534355050508	39.0	35.0	39.0	33.0	39.0
13	36.855638866939906	39.0	35.0	39.0	33.0	39.0
14	37.78954180001857	40.0	37.0	41.0	33.0	41.0
15	38.00425589164341	40.0	37.0	41.0	33.0	41.0
16	38.0734264475432	40.0	37.0	41.0	33.0	41.0
17	38.09350834408676	40.0	37.0	41.0	33.0	41.0
18	38.05336728951243	40.0	36.0	41.0	33.0	41.0
19	37.934708255406555	40.0	36.0	41.0	33.0	41.0
20	37.950723747913266	40.0	36.0	41.0	33.0	41.0
21	37.91448992778627	40.0	36.0	41.0	33.0	41.0
22	38.0089589740383	40.0	36.0	41.0	34.0	41.0
23	38.036965242288325	40.0	36.0	41.0	34.0	41.0
24	37.9883446173203	40.0	36.0	41.0	34.0	41.0
25	37.732993013591944	40.0	35.0	41.0	33.0	41.0
26	37.74297711558175	40.0	35.0	41.0	33.0	41.0
27	37.68410521109867	40.0	35.0	41.0	33.0	41.0
28	37.62347107187455	40.0	35.0	41.0	33.0	41.0
29	37.56898770035946	40.0	35.0	41.0	33.0	41.0
30	37.358224425331464	39.0	35.0	41.0	33.0	41.0
31	37.33650725074327	39.0	35.0	41.0	33.0	41.0
32	37.27372242718465	39.0	35.0	41.0	33.0	41.0
33	37.180996666913	39.0	35.0	41.0	32.0	41.0
34	37.11016430470365	39.0	35.0	41.0	32.0	41.0
35	37.04274840404064	39.0	35.0	41.0	32.0	41.0
36	36.934048838535716	39.0	35.0	41.0	32.0	41.0
37	36.829987929639465	39.0	35.0	41.0	31.0	41.0
38	36.70044851716474	39.0	35.0	41.0	31.0	41.0
39	36.632178027396115	39.0	35.0	41.0	31.0	41.0
40	36.54310937793114	39.0	35.0	41.0	31.0	41.0
41	36.41408158199413	39.0	35.0	41.0	31.0	41.0
42	36.42316561785277	39.0	35.0	41.0	31.0	41.0
43	36.35458247353332	38.0	35.0	41.0	31.0	41.0
44	36.307919255541094	38.0	35.0	40.0	31.0	41.0
45	36.28311532784198	38.0	35.0	40.0	31.0	41.0
46	36.18077307153725	38.0	35.0	40.0	31.0	41.0
47	36.045986747236796	38.0	35.0	40.0	30.0	41.0
48	36.003325507495184	38.0	35.0	40.0	31.0	41.0
49	35.9871318966383	38.0	35.0	40.0	31.0	41.0
50	35.86553769950677	37.0	35.0	40.0	30.0	41.0
51	34.781901659721946	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	20.0
9	40.0
10	29.0
11	25.0
12	18.0
13	14.0
14	18.0
15	26.0
16	35.0
17	87.0
18	148.0
19	291.0
20	529.0
21	876.0
22	1282.0
23	1842.0
24	2466.0
25	3190.0
26	3943.0
27	4603.0
28	5386.0
29	6533.0
30	8405.0
31	11066.0
32	14926.0
33	22530.0
34	39871.0
35	44026.0
36	43394.0
37	65833.0
38	100896.0
39	145275.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31228694487237	19.78913819141659	22.062610494960577	11.835964368750462
2	32.611575039934515	25.462965594735277	24.61387162972606	17.311587735604153
3	32.911912896337014	26.356399659680257	24.500747528607892	16.230939915374833
4	29.885795819524425	23.924326229442965	27.365800139841856	18.824077811190758
5	24.178239622237506	35.53991651176055	21.735933861245805	18.545910004756138
6	25.220800433547648	35.088557032927255	24.705204656089467	14.985437877435626
7	78.10262269796245	5.875442216701816	11.188485217124374	4.833449868211369
8	78.3944336120696	6.857366993911763	9.251163927623313	5.49703546639532
9	73.03269229676033	6.419461135144456	11.35542379850646	9.192422769588756
10	44.81381895217144	21.460418881303067	18.17034556854809	15.555416597977409
11	33.99843483236979	21.776863184263433	23.78069462366814	20.444007359698638
12	29.312974784884194	18.40701558914539	30.75156469391119	21.52844493205922
13	27.41620384318764	20.11808867641012	33.610932676948266	18.85477480345398
14	21.738965662950815	24.782136624354084	32.866246383155314	20.61265132953979
15	19.742145264988945	22.10088699148632	37.428539486374895	20.72842825714984
16	24.871574774651865	21.070074411783096	30.272540024519696	23.785810789045343
17	24.788958178190356	21.387845127989404	27.671443649228124	26.15175304459212
18	25.227053524564226	21.90969399646416	30.07111469874313	22.792137780228483
19	24.587343364807225	23.98590970157597	27.188060764885673	24.23868616873113
20	24.474029776082496	25.639189068838952	27.724121203852665	22.16265995122589
21	24.75977708677964	25.1861242015466	29.733826759060822	20.320271952612938
22	22.23542319214612	21.631905165242667	30.178743659270967	25.953927983340247
23	22.474935526841865	24.27412035115843	29.395591381345703	23.855352740654
24	23.45894466772401	24.83538264179831	27.1602060867209	24.545466603756783
25	24.09732841423507	25.239749194203952	25.771072443006865	24.891849948554114
26	22.048967387288034	22.91928396423232	27.858846892119022	27.172901756360623
27	23.902535154688206	22.525149742581085	29.678875353157526	23.89343974957318
28	21.897756277250686	23.436774617756125	29.121213327042344	25.54425577795084
29	25.633314953035498	23.329903607654543	27.7275319807708	23.309249458539163
30	25.87983832917408	22.89919827793663	28.945747803364917	22.275215589524365
31	28.173775294226882	22.051241238566792	25.522464703196086	24.25251876401024
32	29.652726063451816	24.25687697896119	24.350483856603358	21.739913100983628
33	26.82329712225172	23.60371319913821	25.401003147389144	24.171986531220927
34	25.280110054401895	22.71634273760325	27.06640972147217	24.937137486522694
35	24.852626013995554	25.490251810080363	26.267340484595604	23.38978169132848
36	28.511631696728873	25.558467348443077	24.47611413975469	21.45378681507336
37	24.296858863946003	25.6101974650348	27.292657923708504	22.800285747310696
38	25.03377616586987	26.90003960290977	24.534097347362994	23.53208688385736
39	24.428363262900792	24.68341358133471	24.661054043760267	26.22716911200423
40	24.727185218450785	24.016417206232628	27.52231690286297	23.734080672453615
41	21.66639190963715	24.718468788548886	25.88969168471536	27.725447617098602
42	22.67351853851999	24.513253710641056	28.54138125095928	24.271846499879675
43	23.98837304046129	23.432605890411736	28.048523986288675	24.530497082838295
44	24.705962606515723	21.924474029776082	28.12640339258611	25.243159971122086
45	24.06928424846373	21.92314761653014	26.16653307790404	27.841035057102093
46	25.64013650687177	23.476567015134375	27.311606684364808	23.571689793629048
47	21.925800443022027	23.962413238362146	30.014647391987324	24.097138926628503
48	22.506579957137905	25.740943913563335	27.057693291570267	24.6947828377285
49	24.13844722485926	22.58862809077972	29.34329280193429	23.92963188242673
50	22.72865943202985	22.691330373536918	28.630629913650495	25.949380280782737
51	22.145795554241776	23.065947371712152	26.578479134572202	28.209777939473867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	501.0
2	830.0
3	1671.0
4	2512.0
5	1646.5
6	781.0
7	748.0
8	715.0
9	745.5
10	776.0
11	790.5
12	805.0
13	810.0
14	815.0
15	799.5
16	784.0
17	893.5
18	1003.0
19	980.0
20	957.0
21	1005.5
22	1054.0
23	1261.5
24	1469.0
25	1699.5
26	2338.5
27	2747.0
28	3253.0
29	3759.0
30	4450.5
31	5142.0
32	6132.0
33	7122.0
34	8216.5
35	9311.0
36	10173.5
37	11036.0
38	11910.0
39	12784.0
40	14689.0
41	16594.0
42	19130.5
43	21667.0
44	25366.0
45	29065.0
46	39690.5
47	50316.0
48	51216.5
49	52117.0
50	52119.5
51	52122.0
52	46680.0
53	41238.0
54	38863.0
55	36488.0
56	34968.0
57	33448.0
58	32281.5
59	31115.0
60	27963.5
61	24812.0
62	22568.5
63	20325.0
64	18222.5
65	16120.0
66	14298.0
67	12476.0
68	10403.5
69	8331.0
70	7263.5
71	6196.0
72	5107.0
73	4018.0
74	3305.0
75	1870.5
76	1149.0
77	843.0
78	537.0
79	417.0
80	297.0
81	218.5
82	140.0
83	85.5
84	31.0
85	22.0
86	13.0
87	15.5
88	18.0
89	11.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	527739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.06982685665703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23128724553447	25.331387373343528
2	10.564123770684262	7.4096398306038385
3	4.07892202706707	4.291412677531399
4	2.1664085770823496	3.039022947962189
5	1.3526510774133174	2.3718619541177812
6	0.9636491358163511	2.027700500618797
7	0.8085393682195053	1.984873495517424
8	0.6484851303853407	1.8193808993384446
9	0.5224540727983875	1.6490136486235263
>10	6.544315399326152	42.18208329503997
>50	0.07193783241073676	1.707062496740891
>100	0.04118578191454292	2.858713413991573
>500	0.002196575035442289	0.5498266502927742
>1k	0.0038440063120240057	2.778020816277852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	3164	0.5995387871656255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	2642	0.500626256539691	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	2551	0.48338288434244964	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1990	0.37708033706055455	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.26964086413928096	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1348	0.255429293647049	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1307	0.2476603017779622	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	896	0.16978089548053107	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	733	0.13889441561074697	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	655	0.12411438229882575	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	571	0.10819742334752597	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7897521312618546E-4	0.0	0.0	0.17698142452992863	0.0
2	3.7897521312618546E-4	0.0	0.0	0.9796509259311895	0.0
3	3.7897521312618546E-4	0.0	0.0	1.3059485844328351	0.0
4	3.7897521312618546E-4	0.0	0.0	1.8135858824153606	0.0
5	3.7897521312618546E-4	0.0	0.0	3.5337543747951163	0.0
6	3.7897521312618546E-4	0.0	0.0	4.488203449053414	0.0
7	3.7897521312618546E-4	0.0	0.0	5.425409151114471	0.0
8	3.7897521312618546E-4	0.0	0.0	6.847513638370482	0.0
9	3.7897521312618546E-4	0.0	0.0	7.425450838387915	0.0
10	3.7897521312618546E-4	0.0	0.0	9.057507593715833	0.0
11	3.7897521312618546E-4	0.0	0.0	10.506519321103804	0.0
12	3.7897521312618546E-4	0.0	0.0	12.164535878530865	0.0
13	3.7897521312618546E-4	0.0	0.0	12.683542432907176	0.0
14	3.7897521312618546E-4	0.0	0.0	12.951288420980825	0.0
15	3.7897521312618546E-4	0.0	0.0	13.381614775485609	0.0
16	3.7897521312618546E-4	0.0	0.0	14.104320506917245	0.0
17	3.7897521312618546E-4	0.0	0.0	14.884440983137498	0.0
18	3.7897521312618546E-4	0.0	0.0	15.821836172805117	0.0
19	3.7897521312618546E-4	0.0	0.0	16.437481406528605	0.0
20	3.7897521312618546E-4	0.0	0.0	16.99646984588973	0.0
21	3.7897521312618546E-4	0.0	0.0	17.61040969115415	0.0
22	3.7897521312618546E-4	0.0	0.0	18.276269140616858	0.0
23	3.7897521312618546E-4	0.0	0.0	18.893240787586286	0.0
24	3.7897521312618546E-4	0.0	0.0	19.40012013514256	0.0
25	3.7897521312618546E-4	0.0	0.0	19.850532175943034	0.0
26	3.7897521312618546E-4	0.0	0.0	20.32463016756389	0.0
27	3.7897521312618546E-4	0.0	0.0	20.869596524039345	0.0
28	3.7897521312618546E-4	0.0	0.0	21.33232525926642	0.0
29	3.7897521312618546E-4	0.0	0.0	21.855500540987116	0.0
30	3.7897521312618546E-4	0.0	0.0	22.541635164352076	0.0
31	3.7897521312618546E-4	0.0	0.0	23.05363067728555	0.0
32	3.7897521312618546E-4	0.0	0.0	23.561078487661515	0.0
33	3.7897521312618546E-4	0.0	0.0	24.056588578824	0.0
34	3.7897521312618546E-4	0.0	0.0	24.607429051102912	0.0
35	7.579504262523709E-4	0.0	0.0	25.221558383973896	0.0
36	7.579504262523709E-4	0.0	0.0	25.709299483267298	0.0
37	7.579504262523709E-4	0.0	0.0	26.255592252988695	0.0
38	7.579504262523709E-4	0.0	0.0	26.834855866252067	0.0
39	7.579504262523709E-4	0.0	0.0	27.758039485427457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGG	35	1.2102646E-7	45.000004	2
ATAACGC	25	3.887927E-5	45.000004	11
TCACGAC	45	3.8380676E-10	45.0	25
GGTAATC	20	7.029815E-4	45.0	8
CTATACG	20	7.029815E-4	45.0	1
GAGCGAA	20	7.029815E-4	45.0	8
TGCCTTA	20	7.029815E-4	45.0	35
TATAGGC	20	7.029815E-4	45.0	2
TTCGACG	20	7.029815E-4	45.0	25
TAATACG	120	0.0	44.999996	4
TATAGCG	30	2.1632623E-6	44.999996	1
CGAATAT	70	0.0	41.785717	14
AATACGG	130	0.0	41.538464	5
CTCACGA	50	1.0786607E-9	40.500004	24
TTAACGG	50	1.0786607E-9	40.500004	2
TGCGATG	45	1.9248546E-8	40.0	1
TATACGG	45	1.9248546E-8	40.0	2
AATGCGG	85	0.0	39.705883	2
ATAATAC	165	0.0	39.545452	3
GCGATAC	40	3.4534787E-7	39.375	9
>>END_MODULE