##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702877
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12224471706998	33.0	31.0	34.0	30.0	34.0
2	32.25394770351	34.0	31.0	34.0	30.0	34.0
3	32.156136848979266	33.0	31.0	34.0	30.0	34.0
4	35.80536139324519	37.0	35.0	37.0	35.0	37.0
5	32.25119330978251	37.0	35.0	37.0	0.0	37.0
6	33.97489034354517	37.0	35.0	37.0	17.0	37.0
7	35.591530239287955	37.0	35.0	37.0	32.0	37.0
8	35.84078722166183	37.0	35.0	37.0	35.0	37.0
9	37.78740092505517	39.0	38.0	39.0	35.0	39.0
10	37.40981565764707	39.0	37.0	39.0	34.0	39.0
11	37.388008143672366	39.0	37.0	39.0	35.0	39.0
12	37.330770533108925	39.0	37.0	39.0	35.0	39.0
13	37.30769394929696	39.0	37.0	39.0	34.0	39.0
14	38.516087452000846	40.0	38.0	41.0	34.0	41.0
15	38.65538920750003	40.0	38.0	41.0	35.0	41.0
16	38.58806875171616	40.0	38.0	41.0	34.0	41.0
17	38.58084273635359	40.0	38.0	41.0	34.0	41.0
18	38.441333263145616	40.0	38.0	41.0	34.0	41.0
19	38.25063417923762	40.0	37.0	41.0	34.0	41.0
20	38.30636512505033	40.0	37.0	41.0	34.0	41.0
21	38.189596472782576	40.0	37.0	41.0	34.0	41.0
22	38.26493397849126	40.0	37.0	41.0	34.0	41.0
23	38.26647478861878	40.0	37.0	41.0	34.0	41.0
24	38.22896182404603	40.0	36.0	41.0	34.0	41.0
25	37.941868918743964	40.0	36.0	41.0	34.0	41.0
26	37.99404163175065	40.0	36.0	41.0	34.0	41.0
27	37.98381366867887	40.0	36.0	41.0	34.0	41.0
28	37.911113893327	40.0	36.0	41.0	34.0	41.0
29	37.852750908053615	40.0	36.0	41.0	34.0	41.0
30	37.6758892380886	40.0	35.0	41.0	33.0	41.0
31	37.60590971108743	40.0	35.0	41.0	33.0	41.0
32	37.488685787129185	40.0	35.0	41.0	33.0	41.0
33	37.358914859925704	40.0	35.0	41.0	33.0	41.0
34	37.17475034750034	40.0	35.0	41.0	32.0	41.0
35	37.08465919357156	40.0	35.0	41.0	32.0	41.0
36	36.96718913835564	40.0	35.0	41.0	32.0	41.0
37	36.9009499528367	40.0	35.0	41.0	32.0	41.0
38	36.74166319284882	39.0	35.0	41.0	31.0	41.0
39	36.65366059780018	39.0	35.0	41.0	31.0	41.0
40	36.57319844012537	39.0	35.0	41.0	31.0	41.0
41	36.44124932242768	39.0	35.0	41.0	31.0	41.0
42	36.39159909913114	39.0	35.0	41.0	31.0	41.0
43	36.30893314192953	39.0	35.0	41.0	31.0	41.0
44	36.215111605586756	39.0	35.0	41.0	30.0	41.0
45	36.155923440374345	39.0	35.0	41.0	30.0	41.0
46	36.03282651160872	38.0	35.0	41.0	30.0	41.0
47	35.946738903108226	38.0	35.0	41.0	30.0	41.0
48	35.88515913879669	38.0	35.0	41.0	30.0	41.0
49	35.82956335176709	38.0	35.0	40.0	30.0	41.0
50	35.72427039154788	38.0	35.0	40.0	29.0	41.0
51	34.727535543203146	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	21.0
9	32.0
10	38.0
11	32.0
12	20.0
13	11.0
14	30.0
15	38.0
16	69.0
17	112.0
18	172.0
19	324.0
20	562.0
21	930.0
22	1366.0
23	2109.0
24	3320.0
25	5152.0
26	6972.0
27	8291.0
28	8668.0
29	8680.0
30	10172.0
31	12381.0
32	16267.0
33	24095.0
34	41803.0
35	53834.0
36	56794.0
37	85468.0
38	136283.0
39	218658.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.790417100004696	19.257565690725404	22.13474050225004	15.817276707019861
2	36.78381850594059	21.73196732856531	24.288460143097584	17.19575402239652
3	32.040456580596604	22.423838025714314	28.949304074539356	16.586401319149722
4	28.135363655376405	24.45449203772495	28.15713133307819	19.253012973820454
5	22.301056941683964	35.90599777770506	24.076616534614164	17.716328745996808
6	24.81429894561922	33.41509254108471	26.620873922464384	15.149734590831681
7	78.66895630387678	5.381738198859829	11.32474102865793	4.6245644686054606
8	80.39841963814438	4.179394118743393	10.962800034714466	4.459386208397771
9	75.07615130385544	5.856928025813905	13.199891303883895	5.867029366446761
10	39.19775437238663	24.32602005756341	19.87701973460506	16.599205835444895
11	28.957982691139417	24.117164169548868	26.672234260048345	20.25261887926337
12	24.825538465478314	21.849199788867754	32.22384570842409	21.101416037229843
13	23.77841357733999	22.661432939191354	33.73136409357541	19.828789389893252
14	20.612568059560918	24.94447819462011	33.27082832415913	21.172125421659835
15	19.646396168888725	23.495718312023296	34.91848502654092	21.93940049254706
16	23.631303912348816	23.27434245252014	32.12269002969225	20.97166360543879
17	23.55760680744995	23.538684577813758	30.19504123765609	22.7086673770802
18	24.711862815257863	22.92307188882265	30.30217235732568	22.06289293859381
19	23.5621595243549	25.819311202386764	28.416778469063576	22.20175080419476
20	25.7182977960582	25.53732729908647	27.775414475078854	20.968960429776477
21	25.066690189037345	25.627101185555933	28.965665400916517	20.3405432244902
22	24.23752662272346	22.45158114435385	29.420937091411442	23.889955141511248
23	22.92933187456696	25.29973238560943	29.267994257885803	22.502941481937807
24	22.724317341440965	24.111473273417683	30.473468330874393	22.690741054266965
25	23.87900016645871	24.55991588855518	29.023001179438225	22.538082765547884
26	22.286687428952717	24.134521402748987	29.35165043101425	24.227140737284046
27	22.680497441230827	23.066055654118713	30.443448853782385	23.809998050868074
28	21.480429719566864	24.832936630448856	30.44857066030045	23.238062989683826
29	22.14654911172225	24.693794220041347	29.700075546646143	23.459581121590265
30	23.819103484677974	22.787201743690574	31.54918997207193	21.844504799559523
31	25.397900343872397	23.014695316534755	29.600911681560216	21.986492658032628
32	24.38677037376383	23.881845614524305	28.993550791959333	22.73783321975253
33	23.59616262873874	22.393818548622306	29.708185073633082	24.301833749005873
34	22.622308028289446	23.016118040567555	31.6039648473346	22.757609083808404
35	22.13730140550907	23.70187102437553	30.862156536634433	23.298671033480964
36	24.667900642644447	24.842326609065314	28.70829462338361	21.781478124906634
37	24.08316106516503	23.961944977570756	28.23851114775416	23.716382809510055
38	22.481031531832738	25.900406472256172	28.011159847313255	23.607402148597835
39	23.430699823724492	23.613093044729023	28.13579047258624	24.820416658960244
40	24.072490634919056	22.346726383136737	30.33603318930624	23.244749792637972
41	21.84735024762512	23.701301934762412	29.263299268577576	25.188048549034896
42	23.24716842349373	24.3149228101076	28.985441264972394	23.45246750142628
43	23.13278141125688	22.978415853698444	29.23086116062981	24.657941574414867
44	23.71794780594613	23.296536947431772	29.082755588815683	23.902759657806417
45	23.856094309530686	22.225225750735905	28.99497351599213	24.92370642374128
46	23.51165282119062	22.853500683618897	28.978043100001848	24.656803395188632
47	22.224656661122786	24.393172631911415	29.57359538013052	23.808575326835278
48	22.25794840349023	24.12399324490629	29.811617110817394	23.806441240786082
49	23.79064900402204	22.97983857773124	29.841352043102848	23.388160375143872
50	22.19819399411277	22.960204986078644	30.88363966952966	23.957961350278925
51	22.186812201850394	22.411460326628983	29.508292347025154	25.89343512449547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	768.5
2	1237.0
3	6823.0
4	12409.0
5	7718.0
6	3027.0
7	2922.0
8	2817.0
9	2860.0
10	2903.0
11	2877.0
12	2851.0
13	2751.0
14	2651.0
15	2543.0
16	2435.0
17	2373.0
18	2311.0
19	2383.5
20	2456.0
21	2445.0
22	2434.0
23	2717.5
24	3001.0
25	3365.0
26	4476.0
27	5223.0
28	6020.0
29	6817.0
30	7669.0
31	8521.0
32	10077.0
33	11633.0
34	13073.0
35	14513.0
36	16026.0
37	17539.0
38	18737.0
39	19935.0
40	22247.0
41	24559.0
42	27754.0
43	30949.0
44	34325.5
45	37702.0
46	43741.5
47	49781.0
48	56111.5
49	62442.0
50	62713.0
51	62984.0
52	58063.5
53	53143.0
54	48936.5
55	44730.0
56	43684.0
57	42638.0
58	39971.5
59	37305.0
60	34785.5
61	32266.0
62	29980.5
63	27695.0
64	24474.5
65	21254.0
66	18332.5
67	15411.0
68	13019.5
69	10628.0
70	8843.0
71	7058.0
72	6275.5
73	5493.0
74	4760.5
75	3151.0
76	2274.0
77	1679.5
78	1085.0
79	772.0
80	459.0
81	309.5
82	160.0
83	107.0
84	54.0
85	38.5
86	23.0
87	12.5
88	2.0
89	4.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	702877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.53714458005183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96596893037801	25.2192789814231
2	9.3422942736267	6.639969246225247
3	3.9024611020323965	4.160469732028611
4	2.30298036628242	3.273653849663964
5	1.7410180737571306	3.0935405501795255
6	1.42698147120349	3.04265081131281
7	1.1926776057723847	2.966904955960631
8	1.0748640882895617	3.05580804075614
9	0.9615863763542312	3.0754830674437605
>10	7.004294366006548	39.932182076525166
>50	0.052385941260638995	1.2732797980142687
>100	0.028020386843988596	1.9362569293578074
>500	0.0032487405036508515	0.8761284801469217
>1k	8.121851259127129E-4	0.33884857048014694
>5k	4.0609256295635643E-4	1.1155449104819148
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7730	1.0997656773517983	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT	1190	0.16930415990279948	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	1158	0.16475144299785024	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC	926	0.13174424543696833	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	899	0.12790289054841744	No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	850	0.12093154278771392	No Hit
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	795	0.11310656060733244	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT	754	0.10727339207286624	No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	730	0.1038588543941543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2681720983899033E-4	0.0	0.0	0.10755793687942557	0.0
2	4.2681720983899033E-4	0.0	0.0	0.411736335091346	0.0
3	4.2681720983899033E-4	0.0	0.0	0.730426518437792	0.0
4	4.2681720983899033E-4	0.0	0.0	1.0549498703187044	0.0
5	4.2681720983899033E-4	0.0	0.0	1.9087265623999647	0.0
6	4.2681720983899033E-4	0.0	0.0	3.0214390284502124	0.0
7	4.2681720983899033E-4	0.0	0.0	3.835806264823006	0.0
8	4.2681720983899033E-4	0.0	0.0	5.042703061844391	0.0
9	4.2681720983899033E-4	0.0	0.0	5.733008762557318	0.0
10	4.2681720983899033E-4	0.0	0.0	6.7037333701344615	0.0
11	4.2681720983899033E-4	0.0	0.0	7.699924737898665	0.0
12	7.113620163983172E-4	0.0	0.0	8.515287881094416	0.0
13	7.113620163983172E-4	0.0	0.0	8.878224781860837	0.0
14	7.113620163983172E-4	0.0	0.0	9.068869802255586	0.0
15	7.113620163983172E-4	0.0	0.0	9.295225195873531	0.0
16	7.113620163983172E-4	0.0	0.0	9.707103803368156	0.0
17	7.113620163983172E-4	0.0	0.0	10.198512684296114	0.0
18	7.113620163983172E-4	0.0	0.0	10.744411895680184	0.0
19	7.113620163983172E-4	0.0	0.0	11.047452114665866	0.0
20	7.113620163983172E-4	0.0	0.0	11.369414563287744	0.0
21	7.113620163983172E-4	0.0	0.0	11.741883715073904	0.0
22	7.113620163983172E-4	0.0	0.0	12.1490673332603	0.0
23	7.113620163983172E-4	0.0	0.0	12.579583625584561	0.0
24	7.113620163983172E-4	0.0	0.0	12.934980088977161	0.0
25	7.113620163983172E-4	0.0	0.0	13.241292573238276	0.0
26	7.113620163983172E-4	0.0	0.0	13.522565114522171	0.0
27	7.113620163983172E-4	0.0	0.0	13.841539842675177	0.0
28	8.536344196779807E-4	0.0	0.0	14.15283186105108	0.0
29	8.536344196779807E-4	0.0	0.0	14.500687887069857	0.0
30	8.536344196779807E-4	0.0	0.0	14.970613635102586	0.0
31	8.536344196779807E-4	0.0	0.0	15.325156464075507	0.0
32	8.536344196779807E-4	0.0	0.0	15.699617429507581	0.0
33	8.536344196779807E-4	0.0	0.0	16.06269660267728	0.0
34	9.95906822957644E-4	0.0	0.0	16.433885302833925	0.0
35	9.95906822957644E-4	0.0	0.0	16.844910275908873	0.0
36	9.95906822957644E-4	0.0	0.0	17.211688531563844	0.0
37	9.95906822957644E-4	0.0	0.0	17.565662270923646	0.0
38	0.0011381792262373075	0.0	0.0	17.954635021490247	0.0
39	0.0011381792262373075	0.0	0.0	18.438076647834542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTT	20	7.031413E-4	45.000004	39
CCGGTAT	50	2.1827873E-11	45.000004	42
CGTTTTT	2785	0.0	42.25314	1
ACCGGTA	55	6.002665E-11	40.909092	41
GCGTAAG	55	6.002665E-11	40.909092	1
CGTTATT	370	0.0	40.743244	1
CATACGA	95	0.0	40.263157	18
CGTAAGG	125	0.0	39.6	2
GTTCGAT	40	3.4556615E-7	39.375004	39
AACGGGA	265	0.0	39.056602	4
CGGGTAC	70	0.0	38.571426	6
GTACGAG	70	0.0	38.571426	1
ACGGGAT	200	0.0	38.250004	5
GTAGGGC	295	0.0	38.135593	4
CACGGGA	275	0.0	37.636364	4
CTCGTAG	30	1.1394706E-4	37.500004	1
CGCATCG	30	1.1394706E-4	37.500004	21
GACACGG	110	0.0	36.81818	2
GTTACGG	80	0.0	36.562504	2
AGCGCAA	105	0.0	36.428574	43
>>END_MODULE