##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262975
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91502994581234	33.0	31.0	34.0	30.0	34.0
2	32.02187280159711	33.0	31.0	34.0	30.0	34.0
3	32.019043635326554	33.0	31.0	34.0	30.0	34.0
4	35.67712139937257	37.0	35.0	37.0	35.0	37.0
5	32.23993915771461	37.0	35.0	37.0	16.0	37.0
6	33.88981081851887	37.0	35.0	37.0	19.0	37.0
7	35.3634642076243	37.0	35.0	37.0	32.0	37.0
8	35.46545489114935	37.0	35.0	37.0	33.0	37.0
9	37.35054282726495	39.0	37.0	39.0	34.0	39.0
10	37.10339385873182	39.0	37.0	39.0	34.0	39.0
11	37.094180055138324	39.0	37.0	39.0	34.0	39.0
12	36.97374655385493	39.0	35.0	39.0	33.0	39.0
13	36.77687993155243	39.0	35.0	39.0	32.0	39.0
14	37.98421142694173	40.0	37.0	41.0	33.0	41.0
15	38.02345089837437	40.0	37.0	41.0	33.0	41.0
16	38.18524954843616	40.0	37.0	41.0	34.0	41.0
17	37.94321513451849	40.0	36.0	41.0	33.0	41.0
18	37.96782583895808	40.0	37.0	41.0	33.0	41.0
19	37.86060652153247	40.0	36.0	41.0	33.0	41.0
20	37.53940488639604	40.0	36.0	41.0	32.0	41.0
21	37.766179294609756	40.0	36.0	41.0	33.0	41.0
22	37.76495864625915	40.0	36.0	41.0	33.0	41.0
23	37.63014735240993	40.0	36.0	41.0	33.0	41.0
24	37.67900370757677	40.0	36.0	41.0	33.0	41.0
25	37.65159045536648	40.0	36.0	41.0	33.0	41.0
26	37.524563171404125	40.0	35.0	41.0	33.0	41.0
27	37.3501967867668	40.0	35.0	41.0	32.0	41.0
28	37.29097442722692	40.0	35.0	41.0	32.0	41.0
29	37.28537313432836	40.0	35.0	41.0	32.0	41.0
30	37.1904857876224	40.0	35.0	41.0	32.0	41.0
31	37.25808536933169	40.0	35.0	41.0	32.0	41.0
32	36.937712710333685	40.0	35.0	41.0	31.0	41.0
33	36.85081471622778	40.0	35.0	41.0	31.0	41.0
34	36.4910238615838	39.0	35.0	41.0	30.0	41.0
35	36.470291852837725	39.0	35.0	41.0	30.0	41.0
36	36.354554615457744	39.0	35.0	41.0	30.0	41.0
37	36.13870900275692	39.0	35.0	41.0	30.0	41.0
38	36.24776879931552	39.0	35.0	41.0	29.0	41.0
39	35.87437589124441	39.0	35.0	41.0	28.0	41.0
40	35.869942009696736	39.0	35.0	41.0	28.0	41.0
41	35.79528092023957	39.0	35.0	41.0	26.0	41.0
42	35.700252875748646	39.0	35.0	41.0	27.0	41.0
43	35.62557277307729	39.0	35.0	41.0	27.0	41.0
44	35.73746173590646	39.0	35.0	41.0	28.0	41.0
45	35.806380834680105	39.0	35.0	41.0	28.0	41.0
46	35.67288905789524	39.0	35.0	40.0	28.0	41.0
47	35.48868903888202	38.0	35.0	40.0	27.0	41.0
48	35.49108470386919	38.0	35.0	40.0	27.0	41.0
49	35.4723680958266	38.0	35.0	40.0	27.0	41.0
50	35.24447190797604	38.0	35.0	40.0	26.0	41.0
51	34.26924992870045	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	11.0
11	11.0
12	10.0
13	5.0
14	4.0
15	9.0
16	15.0
17	39.0
18	92.0
19	260.0
20	670.0
21	1269.0
22	2143.0
23	2527.0
24	2715.0
25	2713.0
26	2738.0
27	2575.0
28	2748.0
29	2981.0
30	3977.0
31	5236.0
32	7173.0
33	10213.0
34	16286.0
35	21444.0
36	19687.0
37	29513.0
38	48787.0
39	77062.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.95750546629908	15.811769179579807	19.678296416009125	11.552428938111987
2	27.9650156859017	35.34442437494059	21.451088506512026	15.239471432645688
3	28.279113984219034	35.16189751877555	22.55879836486358	14.000190132141837
4	25.91577146116551	21.797129004658235	35.510980131191175	16.776119402985074
5	30.377792565833257	32.44833159045537	19.594258009316476	17.579617834394902
6	22.233672402319613	41.912349082612415	22.38083468010267	13.4731438349653
7	69.87356212567735	5.184903507938017	20.33197071965016	4.609563646734481
8	68.43958551193079	15.882498336343758	9.942389961022911	5.735526190702538
9	64.13119117786862	6.41315714421523	10.566023386253447	18.889628291662707
10	42.827645213423324	22.572868143359635	18.83829261336629	15.761194029850747
11	35.41515353170453	21.818043540260483	23.397661374655385	19.3691415533796
12	26.806730677821083	19.180910732959408	28.54111607567259	25.471242513546915
13	27.52238805970149	22.34128719460025	32.82213138131001	17.31419336438825
14	20.10837532084799	33.345375035649774	25.417625249548436	21.1286243939538
15	16.61070443958551	23.21247266850461	36.717178439015115	23.45964445289476
16	18.366764901606615	23.255062268276454	28.66736381785341	29.71080901226352
17	18.53902462211237	26.65234337864816	30.000190132141842	24.808441867097635
18	19.690845137370474	23.32160851791995	29.25791425040403	27.72963209430554
19	20.525905504325507	25.672402319612132	26.05190607472193	27.74978610134043
20	26.902177013024055	23.713280730107424	30.310485787622397	19.074056469246127
21	21.919574104002283	32.555946382736	26.31466869474285	19.20981081851887
22	19.37028234623063	22.526475900751024	30.550052286339007	27.55318946667934
23	22.316950280444907	29.204677250689233	27.075197262097156	21.403175206768704
24	22.13480368856355	26.712425135469154	26.310105523338724	24.842665652628575
25	18.785435877935164	33.99562696073771	24.899705295180148	22.31923186614697
26	18.24508033082993	24.325125962543968	32.19051240612226	25.23928130050385
27	25.1335678296416	27.07709858351554	25.166270558037834	22.623063028805017
28	17.581138891529612	27.89048388630098	31.012833919574106	23.515543302595304
29	21.916531989732864	22.115790474379693	32.4460500047533	23.521627531134136
30	20.20039927749786	32.590170168266944	27.298412396615646	19.911018157619544
31	23.046297176537696	23.91482080045632	23.66955033748455	29.369331685521438
32	29.91691225401654	27.421618024527046	23.339100674969103	19.32236904648731
33	27.69236619450518	23.355452039167222	22.605951136039547	26.346230630288048
34	22.246981652248312	22.925373134328357	30.05190607472193	24.775739138701397
35	24.160091263428082	27.42960357448427	24.88905789523719	23.521247266850462
36	24.000760528567355	27.124631618975187	26.91586652723643	21.95874132522103
37	22.858826884684856	28.014069778496054	27.930031371803405	21.197071965015684
38	23.270653103907215	25.4199068352505	22.46639414393003	28.843045916912253
39	27.013594448141458	23.142123776024338	27.707196501568593	22.137085274265615
40	23.91482080045632	24.43958551193079	28.52780682574389	23.117786861869
41	21.439300313718036	24.44414868333492	27.779446715467248	26.3371042874798
42	20.790949710048483	26.76718319231866	28.994391101815765	23.447475995817094
43	26.776309535126913	24.104952942294894	24.67801121779637	24.440726304781823
44	22.847038691890866	22.745888392432743	29.678486548150968	24.72858636752543
45	21.368190892670405	21.97357163228444	27.287764996672685	29.370472478372466
46	28.3878695693507	25.47694647780207	25.479228063504134	20.655955889343094
47	19.6071869949615	25.721456412206482	34.23861583800741	20.432740754824604
48	22.317710809012265	25.448046392242606	26.855404506131762	25.378838292613366
49	21.716512976518683	22.09639699591216	33.182241657952275	23.004848369616884
50	23.842570586557656	21.744272269227114	29.526000570396427	24.887156573818803
51	21.305827550147352	21.635896948379123	27.61859492347181	29.43968057800171
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	282.5
2	463.0
3	830.5
4	1198.0
5	823.0
6	448.0
7	423.5
8	399.0
9	402.5
10	406.0
11	433.0
12	460.0
13	466.5
14	473.0
15	491.5
16	510.0
17	500.0
18	490.0
19	547.5
20	605.0
21	722.0
22	839.0
23	890.0
24	941.0
25	1102.0
26	1497.0
27	1731.0
28	2131.0
29	2531.0
30	2977.0
31	3423.0
32	4035.5
33	4648.0
34	5042.5
35	5437.0
36	5924.5
37	6412.0
38	7001.0
39	7590.0
40	8463.5
41	9337.0
42	10834.0
43	12331.0
44	15002.5
45	17674.0
46	29416.0
47	41158.0
48	33310.0
49	25462.0
50	25297.0
51	25132.0
52	22080.0
53	19028.0
54	17482.5
55	15937.0
56	14364.0
57	12791.0
58	11726.0
59	10661.0
60	10079.0
61	9497.0
62	8749.5
63	8002.0
64	6682.0
65	5362.0
66	4500.5
67	3639.0
68	3003.5
69	2368.0
70	1951.0
71	1534.0
72	1407.0
73	1280.0
74	1003.0
75	549.0
76	372.0
77	278.0
78	184.0
79	133.0
80	82.0
81	55.0
82	28.0
83	21.0
84	14.0
85	9.5
86	5.0
87	2.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.10390721551478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79117387828725	27.379408689038883
2	9.107959087461824	6.758817378077763
3	3.6013692172095024	4.008746078524575
4	2.1112181523766576	3.1333776974997627
5	1.4553057166868224	2.699876414107805
6	1.2810789759567098	2.851982127578667
7	1.0545842130075636	2.739043635326552
8	0.9213519995080657	2.7348607282061033
9	0.8865066513620432	2.9603574484266564
>10	5.689015516428557	31.288145260956362
>50	0.057392338122860605	1.466299077859112
>100	0.029721032242195664	2.035554710523814
>500	0.007174042265357576	1.9199543682859588
>1k	0.003074589542296104	1.5309440060842285
>5k	0.003074589542296104	6.492632379503756
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	6050	2.3005989162467917	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	5570	2.118072060081757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	5454	2.0739614031752067	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1926	0.7323890103622017	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	1065	0.40498146211617075	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	1035	0.39357353360585606	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	896	0.34071679817473144	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	874	0.332350983933834	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	829	0.315239091168362	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	720	0.27379028424755203	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.23918623443293088	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	552	0.2099058845897899	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	549	0.20876509173875843	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTT	457	0.17378077764046013	No Hit
GAATATGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	447	0.16997813480368856	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	445	0.16921760623633425	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	283	0.10761479228063504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17796368476090882	0.0
2	0.0	0.0	0.0	1.277687993155243	0.0
3	0.0	0.0	0.0	1.5609848844947238	0.0
4	0.0	0.0	0.0	2.206293373894857	0.0
5	0.0	0.0	0.0	5.2993630573248405	0.0
6	0.0	0.0	0.0	6.084608803118167	0.0
7	0.0	0.0	0.0	7.114364483315905	0.0
8	0.0	0.0	0.0	8.347181290997243	0.0
9	0.0	0.0	0.0	8.692841524859778	0.0
10	0.0	0.0	0.0	11.811008651012454	0.0
11	0.0	0.0	0.0	12.928225116455938	0.0
12	0.0	0.0	0.0	16.474950090312767	0.0
13	0.0	0.0	0.0	17.018347751687422	0.0
14	0.0	0.0	0.0	17.2700827074817	0.0
15	0.0	0.0	0.0	17.951516303831163	0.0
16	0.0	0.0	0.0	18.640935450137846	0.0
17	0.0	0.0	0.0	19.374845517634757	0.0
18	0.0	0.0	0.0	20.15895047057705	0.0
19	0.0	0.0	0.0	21.11113223690465	0.0
20	0.0	0.0	0.0	21.614221884209524	0.0
21	0.0	0.0	0.0	22.11997338150014	0.0
22	3.802642836771556E-4	0.0	0.0	22.77288715657382	0.0
23	3.802642836771556E-4	0.0	0.0	23.326932217891436	0.0
24	3.802642836771556E-4	0.0	0.0	23.7619545584181	0.0
25	3.802642836771556E-4	0.0	0.0	24.130050385017586	0.0
26	3.802642836771556E-4	0.0	0.0	24.512596254396804	0.0
27	3.802642836771556E-4	0.0	0.0	24.99591215895047	0.0
28	3.802642836771556E-4	0.0	0.0	25.360205342713186	0.0
29	3.802642836771556E-4	0.0	0.0	25.74199068352505	0.0
30	3.802642836771556E-4	0.0	0.0	26.19146306683145	0.0
31	3.802642836771556E-4	0.0	0.0	26.59226162182717	0.0
32	3.802642836771556E-4	0.0	0.0	26.95579427702253	0.0
33	3.802642836771556E-4	0.0	0.0	27.336058560699687	0.0
34	3.802642836771556E-4	0.0	0.0	27.711379408689037	0.0
35	3.802642836771556E-4	0.0	0.0	28.139556992109515	0.0
36	3.802642836771556E-4	0.0	0.0	28.53617263998479	0.0
37	3.802642836771556E-4	0.0	0.0	28.91833824508033	0.0
38	3.802642836771556E-4	0.0	0.0	29.269322178914347	0.0
39	3.802642836771556E-4	0.0	0.0	29.640840384066927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	30	2.159095E-6	45.000004	2
TTTCGAC	30	2.159095E-6	45.000004	24
ACGGGCG	30	2.159095E-6	45.000004	5
TCGACGT	30	2.159095E-6	45.000004	26
AGTACGG	30	2.159095E-6	45.000004	2
CTAATGG	30	2.159095E-6	45.000004	2
GTACGAG	30	2.159095E-6	45.000004	1
CGGCGAA	210	0.0	45.0	31
CGAACGC	40	6.7811925E-9	45.0	34
GCGACAA	25	3.8825747E-5	45.0	11
CAACGAT	25	3.8825747E-5	45.0	42
CGTGCGT	20	7.0233556E-4	45.0	11
GGCGTGC	35	1.2071541E-7	45.0	9
TAAACGG	20	7.0233556E-4	45.0	2
GATCACC	20	7.0233556E-4	45.0	35
AGGGTCC	20	7.0233556E-4	45.0	6
AGGGTAC	20	7.0233556E-4	45.0	6
TATGCGG	25	3.8825747E-5	45.0	2
TACGCGG	20	7.0233556E-4	45.0	2
GCGATAT	25	3.8825747E-5	45.0	9
>>END_MODULE