FastQCFastQC Report
Sat 14 Jan 2017
SRR2936417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610256
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.45816837523924386No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC11950.19581945937442646TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC8610.14108833014341524TruSeq Adapter, Index 19 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC7980.130764793791458No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT7660.1255210927872893TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG207.0306833E-445.020
TTGTCGG253.888648E-545.02
TACGAAT502.1827873E-1145.012
CGAATAT502.1827873E-1145.014
ACGCTTG207.0306833E-445.017
TATAGCG406.8066583E-945.01
CGGTCTA253.888648E-545.031
GCGTAAG551.8189894E-1245.01
TTACGGG1350.041.6666683
TTAACGG650.041.5384642
CGTTTTT13200.041.251
ACGGGAT2250.041.05
GTTACGG403.454661E-739.3752
CGTGCGG1100.038.8636362
CGTAAGG1050.038.571432
ACGGGCG1350.038.3333365
CTACGAA659.094947E-1238.07692711
CGTTATT1800.037.5000041
CGGTAGT1500.037.512
ATTTACG301.1392946E-437.4999961
Produced by FastQC (version 0.11.5)