##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	610256
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09471762670093	33.0	31.0	34.0	30.0	34.0
2	32.201335177368186	33.0	31.0	34.0	30.0	34.0
3	32.14934224325529	33.0	31.0	34.0	30.0	34.0
4	35.78230611415537	37.0	35.0	37.0	35.0	37.0
5	31.979892045305576	37.0	35.0	37.0	0.0	37.0
6	33.82673500956975	37.0	35.0	37.0	17.0	37.0
7	35.536912377756224	37.0	35.0	37.0	32.0	37.0
8	35.809674956084	37.0	35.0	37.0	35.0	37.0
9	37.757414920951206	39.0	38.0	39.0	35.0	39.0
10	37.35417267507407	39.0	37.0	39.0	34.0	39.0
11	37.331760769250934	39.0	37.0	39.0	34.0	39.0
12	37.30287780865735	39.0	37.0	39.0	34.0	39.0
13	37.27635779082877	39.0	37.0	39.0	34.0	39.0
14	38.46004135969167	40.0	38.0	41.0	34.0	41.0
15	38.6081415012716	40.0	38.0	41.0	34.0	41.0
16	38.538020109593354	40.0	38.0	41.0	34.0	41.0
17	38.548283015652444	40.0	38.0	41.0	34.0	41.0
18	38.455962743504365	40.0	38.0	41.0	34.0	41.0
19	38.312355798222384	40.0	37.0	41.0	34.0	41.0
20	38.40090388296059	40.0	37.0	41.0	34.0	41.0
21	38.31059915838599	40.0	37.0	41.0	34.0	41.0
22	38.39002320337694	40.0	37.0	41.0	34.0	41.0
23	38.399160352376704	40.0	37.0	41.0	34.0	41.0
24	38.36434709367872	40.0	37.0	41.0	34.0	41.0
25	38.101386958915604	40.0	37.0	41.0	34.0	41.0
26	38.168154020607744	40.0	37.0	41.0	34.0	41.0
27	38.15032543719357	40.0	37.0	41.0	34.0	41.0
28	38.081364214362495	40.0	37.0	41.0	34.0	41.0
29	38.05567171809864	40.0	37.0	41.0	34.0	41.0
30	37.890981817466766	40.0	36.0	41.0	34.0	41.0
31	37.876610799402215	40.0	37.0	41.0	33.0	41.0
32	37.7995890249338	40.0	36.0	41.0	33.0	41.0
33	37.70580543247424	40.0	36.0	41.0	33.0	41.0
34	37.61883045803728	40.0	36.0	41.0	33.0	41.0
35	37.58329455179466	40.0	36.0	41.0	33.0	41.0
36	37.48047540704229	40.0	36.0	41.0	33.0	41.0
37	37.42926247345376	40.0	36.0	41.0	33.0	41.0
38	37.277426194908365	40.0	35.0	41.0	33.0	41.0
39	37.19716479641331	40.0	35.0	41.0	32.0	41.0
40	37.12168991374112	40.0	35.0	41.0	32.0	41.0
41	37.02112064445085	40.0	35.0	41.0	32.0	41.0
42	37.00345264937993	40.0	35.0	41.0	32.0	41.0
43	36.92778768254634	39.0	35.0	41.0	32.0	41.0
44	36.85214074093495	39.0	35.0	41.0	32.0	41.0
45	36.81546924569361	39.0	35.0	41.0	32.0	41.0
46	36.701920177761465	39.0	35.0	41.0	31.0	41.0
47	36.64605018221861	39.0	35.0	41.0	31.0	41.0
48	36.592538213471066	39.0	35.0	41.0	31.0	41.0
49	36.55431327180724	39.0	35.0	41.0	31.0	41.0
50	36.44738929236255	39.0	35.0	41.0	31.0	41.0
51	35.42744028735481	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	28.0
10	27.0
11	23.0
12	15.0
13	9.0
14	22.0
15	28.0
16	41.0
17	63.0
18	139.0
19	255.0
20	391.0
21	684.0
22	1093.0
23	1641.0
24	2217.0
25	3337.0
26	4583.0
27	5226.0
28	6055.0
29	6520.0
30	8070.0
31	10279.0
32	13580.0
33	19933.0
34	34134.0
35	44324.0
36	46935.0
37	74090.0
38	122121.0
39	204237.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.919089693505676	19.273550772134975	22.46663695236098	12.340722581998374
2	34.724935109200075	22.719973257124877	24.34486510579167	18.210226527883382
3	33.78729582339215	23.332011483705198	26.33124459243334	16.54944810046931
4	28.66649406150861	25.603844943761306	25.46226501664875	20.26739597808133
5	22.13267874465798	37.348751999161	22.595926955245012	17.922642300936
6	25.123718570567107	35.026611782596156	24.840886447654757	15.008783199181982
7	81.20395375055715	5.401339765606565	9.12371201594085	4.270994467895441
8	82.09210560813823	4.573490469573425	8.682913400277917	4.651490522010435
9	76.1529587582916	6.760933116593691	10.787767756482525	6.298340368632181
10	37.05936524999345	29.488608059568445	17.798759864712512	15.653266825725598
11	29.123351511496814	25.41818515508246	26.161479772423373	19.29698356099735
12	25.721008888073204	23.88767992449071	30.4101885110511	19.981122676384995
13	25.03850842924936	23.87211268713458	32.049992134448495	19.039386749167562
14	21.453455598961746	27.350325109462258	30.78249128234708	20.41372800922891
15	20.4661650192706	25.661689520463543	33.59803099027294	20.27411446999292
16	23.92225557798694	25.115033690778954	30.694987021840014	20.26772370939409
17	24.006318659710026	24.215247371594874	29.079271649930522	22.699162318764586
18	25.194344668467007	23.785100023596655	29.96627644791694	21.0542788600194
19	24.261129755381347	25.98319393828164	27.83651451194253	21.919161794394483
20	25.2151556068273	26.15148396738418	27.79456490390918	20.83879552187934
21	24.507092105608137	26.83037938176765	28.935069872315882	19.727458640308328
22	24.026965732413938	23.733318476180486	28.815611808814662	23.424103982590914
23	21.69581290474817	27.246762014629926	28.831506777483547	22.225918303138357
24	21.865249993445374	25.373777562203404	30.129814372984455	22.631158071366773
25	22.719809391468498	26.552135497233948	27.987107050156002	22.74094806114155
26	21.86443066516347	26.238660496578486	28.46788888597572	23.42901995228232
27	22.550208437114915	25.211386696730553	29.219049054822893	23.01935581133164
28	20.555799533310612	26.067420885661104	30.44869694030046	22.928082640727826
29	21.95799795495661	25.45030282373299	29.47844838887287	23.113250832437533
30	23.00247764872447	24.53888204294591	29.50270050601715	22.95593980231247
31	23.220255106053852	25.12650428672557	28.124262604546292	23.528978002674286
32	23.569616685456594	25.42801709446527	28.51606538895152	22.48630083112661
33	22.868107810492646	25.58860543771794	27.388014210429724	24.155272541359693
34	21.440674077764086	25.567466768044888	29.903188170210537	23.088670983980496
35	21.961111402427832	25.1196219291576	29.54825515849086	23.371011509923704
36	22.96183896594216	27.10960646023964	27.929917936079285	21.998636637738915
37	22.472372250334285	26.31240004194961	28.093291995490414	23.12193571222569
38	22.22264099001075	27.280518339844264	28.1183634409166	22.378477229228388
39	23.772318502398992	25.02884035552293	27.060446763325558	24.138394378752523
40	24.076780891953543	24.175100285781706	29.410771872787812	22.33734694947694
41	21.203396607325452	24.475629899583126	29.114338900395897	25.206634592695526
42	23.182729870742772	24.7491216800818	29.047153981279987	23.020994467895438
43	23.409356073516687	24.16477674942975	28.928351380404287	23.497515796649274
44	22.857948139797067	24.691768700348707	28.745968904853047	23.70431425500118
45	22.856637214546026	24.10709603838389	28.06527096824939	24.970995778820694
46	23.17732230408222	24.803688943656432	27.961216276447914	24.05777247581343
47	21.65206077449464	25.025399176738944	29.695406517920347	23.62713353084607
48	21.801014656144307	25.02818489289741	29.385372696048872	23.785427754909417
49	23.31382239584699	23.80558323064419	29.446658451535097	23.433935921973728
50	21.336619385962614	24.83121837392832	29.51023832621064	24.32192391389843
51	21.46935056763063	23.901936236595787	28.2637122781259	26.365000917647674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	522.5
2	861.0
3	2842.5
4	4824.0
5	3143.0
6	1462.0
7	1442.5
8	1423.0
9	1427.5
10	1432.0
11	1536.0
12	1640.0
13	1530.0
14	1420.0
15	1479.5
16	1539.0
17	1492.0
18	1445.0
19	1501.0
20	1557.0
21	1812.0
22	2067.0
23	2306.0
24	2545.0
25	2944.0
26	4185.0
27	5027.0
28	5729.5
29	6432.0
30	7401.5
31	8371.0
32	9765.0
33	11159.0
34	13167.5
35	15176.0
36	16429.0
37	17682.0
38	19727.5
39	21773.0
40	23778.0
41	25783.0
42	29196.0
43	32609.0
44	35490.0
45	38371.0
46	44677.5
47	50984.0
48	55287.5
49	59591.0
50	59745.5
51	59900.0
52	54500.5
53	49101.0
54	44502.5
55	39904.0
56	36020.0
57	32136.0
58	30558.0
59	28980.0
60	25771.0
61	22562.0
62	20522.5
63	18483.0
64	15909.0
65	13335.0
66	11389.0
67	9443.0
68	7844.5
69	6246.0
70	5223.0
71	4200.0
72	3339.5
73	2479.0
74	2396.5
75	1778.5
76	1243.0
77	938.5
78	634.0
79	492.5
80	351.0
81	256.5
82	162.0
83	107.5
84	53.0
85	33.5
86	14.0
87	11.5
88	9.0
89	6.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	610256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.51349472271016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69737706247278	26.179217990027603
2	9.821418705530368	7.172286401478201
3	3.8947558220801497	4.266334384673046
4	2.1756438330452212	3.1776143846557434
5	1.5751192565473286	2.8756554330790025
6	1.174477326415028	2.573056299599872
7	1.0087426798906391	2.5782904363131554
8	0.8810861369704341	2.5737227210018427
9	0.8030465965926661	2.6389833900099
>10	6.883720569540852	41.64204445259772
>50	0.057011301475508896	1.4122394048054199
>100	0.024885885559781207	1.7590211676007832
>500	0.001809882586165906	0.49216906718162234
>1k	9.04941293082953E-4	0.6593644669761177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2796	0.45816837523924386	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	1195	0.19581945937442646	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	861	0.14108833014341524	TruSeq Adapter, Index 19 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	798	0.130764793791458	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	766	0.1255210927872893	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.277313127605464E-4	0.0	0.0	0.12994546550955666	0.0
2	3.277313127605464E-4	0.0	0.0	0.5738575286437168	0.0
3	3.277313127605464E-4	0.0	0.0	0.8517736818646601	0.0
4	3.277313127605464E-4	0.0	0.0	1.2035932461131067	0.0
5	3.277313127605464E-4	0.0	0.0	2.221035106578223	0.0
6	3.277313127605464E-4	0.0	0.0	3.155560944914921	0.0
7	3.277313127605464E-4	0.0	0.0	3.884926981463517	0.0
8	3.277313127605464E-4	0.0	0.0	5.1094950315932985	0.0
9	3.277313127605464E-4	0.0	0.0	5.654184473401327	0.0
10	3.277313127605464E-4	0.0	0.0	6.7240633439081305	0.0
11	3.277313127605464E-4	0.0	0.0	7.907501114286464	0.0
12	3.277313127605464E-4	0.0	0.0	8.897905141448835	0.0
13	3.277313127605464E-4	0.0	0.0	9.323300385412024	0.0
14	3.277313127605464E-4	0.0	0.0	9.542388767992449	0.0
15	3.277313127605464E-4	0.0	0.0	9.860288141370178	0.0
16	3.277313127605464E-4	0.0	0.0	10.431523819511812	0.0
17	3.277313127605464E-4	0.0	0.0	11.130410843973676	0.0
18	4.915969691408196E-4	0.0	0.0	11.887634042106919	0.0
19	4.915969691408196E-4	0.0	0.0	12.339411656747332	0.0
20	4.915969691408196E-4	0.0	0.0	12.801512807739703	0.0
21	4.915969691408196E-4	0.0	0.0	13.328668624315041	0.0
22	4.915969691408196E-4	0.0	0.0	13.908589182244828	0.0
23	6.554626255210928E-4	0.0	0.0	14.451148370519913	0.0
24	6.554626255210928E-4	0.0	0.0	14.896535224561495	0.0
25	6.554626255210928E-4	0.0	0.0	15.300136336226108	0.0
26	6.554626255210928E-4	0.0	0.0	15.689481135785638	0.0
27	6.554626255210928E-4	0.0	0.0	16.11733436459453	0.0
28	8.19328281901366E-4	0.0	0.0	16.549611966125692	0.0
29	8.19328281901366E-4	0.0	0.0	16.991721507039667	0.0
30	8.19328281901366E-4	0.0	0.0	17.573444587189638	0.0
31	8.19328281901366E-4	0.0	0.0	18.02849951495766	0.0
32	8.19328281901366E-4	0.0	0.0	18.503054455834928	0.0
33	8.19328281901366E-4	0.0	0.0	18.956306861382764	0.0
34	8.19328281901366E-4	0.0	0.0	19.412508848745446	0.0
35	8.19328281901366E-4	0.0	0.0	19.88280328255683	0.0
36	8.19328281901366E-4	0.0	0.0	20.330484255787734	0.0
37	8.19328281901366E-4	0.0	0.0	20.76538370782098	0.0
38	8.19328281901366E-4	0.0	0.0	21.227976455782493	0.0
39	8.19328281901366E-4	0.0	0.0	21.792002045043393	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	20	7.0306833E-4	45.0	20
TTGTCGG	25	3.888648E-5	45.0	2
TACGAAT	50	2.1827873E-11	45.0	12
CGAATAT	50	2.1827873E-11	45.0	14
ACGCTTG	20	7.0306833E-4	45.0	17
TATAGCG	40	6.8066583E-9	45.0	1
CGGTCTA	25	3.888648E-5	45.0	31
GCGTAAG	55	1.8189894E-12	45.0	1
TTACGGG	135	0.0	41.666668	3
TTAACGG	65	0.0	41.538464	2
CGTTTTT	1320	0.0	41.25	1
ACGGGAT	225	0.0	41.0	5
GTTACGG	40	3.454661E-7	39.375	2
CGTGCGG	110	0.0	38.863636	2
CGTAAGG	105	0.0	38.57143	2
ACGGGCG	135	0.0	38.333336	5
CTACGAA	65	9.094947E-12	38.076927	11
CGTTATT	180	0.0	37.500004	1
CGGTAGT	150	0.0	37.5	12
ATTTACG	30	1.1392946E-4	37.499996	1
>>END_MODULE