FastQCFastQC Report
Sat 14 Jan 2017
SRR2936416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816816
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115851.418312080076786No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT30050.36789191200955906RNA PCR Primer, Index 11 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC25930.3174521556874498RNA PCR Primer, Index 11 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC23790.2912528647822766TruSeq Adapter, Index 11 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG20150.24668958492487905TruSeq Adapter, Index 11 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT16480.20175902528843706RNA PCR Primer, Index 11 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.16637773990715166No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC13420.1642964878258996Illumina PCR Primer Index 11 (100% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT12980.15890971773324714TruSeq Adapter, Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC12670.15511449334978747RNA PCR Primer, Index 11 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT10110.12377328553799141TruSeq Adapter, Index 11 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC207.032087E-445.024
TCGACGT207.032087E-445.026
CGTTATT5900.043.4745751
CGTAAGG1350.043.3333362
CGTTTTT47700.042.97171
CGCACGG1100.042.9545442
TTACGGG1900.040.2631573
TACGGGA2800.039.3754
TCACGGG1150.039.1304363
CGTTTCT4200.039.1071431
GCGAGAC5400.037.91666821
ACACGAC5250.037.71428326
CCTTAAC301.1396327E-437.49999634
ATTCGCG301.1396327E-437.4999961
CACGACC5300.037.3584927
AGTACGG850.037.0588232
AACACGT4650.036.77419341
AGACACG5400.036.66666824
ACGACCA5350.036.58878328
TCAAGCG5600.036.562517
Produced by FastQC (version 0.11.5)