##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	816816
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995487355781474	33.0	31.0	34.0	30.0	34.0
2	32.13318911480676	33.0	31.0	34.0	30.0	34.0
3	32.00070517717577	33.0	31.0	34.0	30.0	34.0
4	35.687793823823235	37.0	35.0	37.0	33.0	37.0
5	32.190700231141406	37.0	35.0	37.0	0.0	37.0
6	33.905849052907875	37.0	35.0	37.0	17.0	37.0
7	35.49582402891227	37.0	35.0	37.0	32.0	37.0
8	35.750439511468926	37.0	35.0	37.0	35.0	37.0
9	37.676659125188536	39.0	37.0	39.0	35.0	39.0
10	37.34057609057609	39.0	37.0	39.0	34.0	39.0
11	37.31360306360306	39.0	37.0	39.0	34.0	39.0
12	37.2852845683728	39.0	37.0	39.0	34.0	39.0
13	37.285427807486634	39.0	37.0	39.0	34.0	39.0
14	38.5089175530352	40.0	38.0	41.0	34.0	41.0
15	38.627297947150886	40.0	38.0	41.0	34.0	41.0
16	38.545441078529315	40.0	38.0	41.0	34.0	41.0
17	38.51931157078216	40.0	38.0	41.0	34.0	41.0
18	38.3633009147715	40.0	38.0	41.0	34.0	41.0
19	38.136377103288865	40.0	37.0	41.0	34.0	41.0
20	38.15940187263717	40.0	37.0	41.0	34.0	41.0
21	38.05384321560792	40.0	36.0	41.0	34.0	41.0
22	38.13465945818887	40.0	36.0	41.0	34.0	41.0
23	38.14838470353176	40.0	36.0	41.0	34.0	41.0
24	38.10188463497287	40.0	36.0	41.0	34.0	41.0
25	37.80341472253237	40.0	36.0	41.0	33.0	41.0
26	37.86259941039353	40.0	36.0	41.0	34.0	41.0
27	37.84847138155962	40.0	36.0	41.0	34.0	41.0
28	37.71166823372706	40.0	35.0	41.0	33.0	41.0
29	37.61600654247713	40.0	35.0	41.0	33.0	41.0
30	37.41623572505925	40.0	35.0	41.0	33.0	41.0
31	37.292314303343716	40.0	35.0	41.0	33.0	41.0
32	37.09643910011557	39.0	35.0	41.0	32.0	41.0
33	36.92350908895027	40.0	35.0	41.0	31.0	41.0
34	36.69232728056257	39.0	35.0	41.0	31.0	41.0
35	36.61172406025347	39.0	35.0	41.0	31.0	41.0
36	36.49001733560557	39.0	35.0	41.0	30.0	41.0
37	36.4293904624787	39.0	35.0	41.0	30.0	41.0
38	36.26470220955515	39.0	35.0	41.0	30.0	41.0
39	36.191356437679964	39.0	35.0	41.0	30.0	41.0
40	36.11915290591761	39.0	35.0	41.0	29.0	41.0
41	35.97131422499069	39.0	35.0	41.0	29.0	41.0
42	35.945114934085524	39.0	35.0	41.0	29.0	41.0
43	35.84992091242091	39.0	35.0	41.0	28.0	41.0
44	35.75377808466044	39.0	35.0	41.0	28.0	41.0
45	35.695861736302916	38.0	35.0	41.0	28.0	41.0
46	35.56265670603906	38.0	35.0	40.0	27.0	41.0
47	35.48567119155354	38.0	35.0	40.0	27.0	41.0
48	35.42277208086032	38.0	35.0	40.0	27.0	41.0
49	35.35961832285362	38.0	35.0	40.0	27.0	41.0
50	35.22951190965897	38.0	35.0	40.0	26.0	41.0
51	34.18823578382402	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	26.0
9	53.0
10	32.0
11	26.0
12	17.0
13	32.0
14	28.0
15	43.0
16	94.0
17	148.0
18	247.0
19	493.0
20	799.0
21	1308.0
22	1999.0
23	3106.0
24	4827.0
25	7560.0
26	10504.0
27	12111.0
28	12347.0
29	12486.0
30	13484.0
31	16038.0
32	20173.0
33	28852.0
34	46837.0
35	60078.0
36	67219.0
37	99967.0
38	159270.0
39	236464.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.467111320052496	18.587295057883292	21.556629644864937	18.38896397719927
2	38.17187714246538	22.30722708663885	22.155785391079508	17.36511037981626
3	31.408420011361187	22.606804959746135	30.0057785351903	15.978996493702377
4	27.09851423086717	24.033074768368888	30.14828308945956	18.72012791130438
5	21.8477600830542	35.510690290102055	25.46228281522399	17.179266811619755
6	23.66016336604572	32.67737164795989	28.286786742669097	15.375678243325302
7	76.50499010793128	5.092823842823843	13.824533309827428	4.577652739417445
8	77.4926054337819	4.505788329317741	13.186445907034141	4.815160329866212
9	71.37580556698204	6.31819161230926	15.384982664394428	6.921020156314274
10	35.36683904330963	27.811159428806487	21.043539793539793	15.778461734344088
11	26.766738163796987	23.721499089146146	30.103107676637087	19.408655070419776
12	23.544102955867665	21.182738829797653	34.190564337623165	21.082593876711524
13	22.62308769661711	22.161539440951206	36.06699672876144	19.14837613367025
14	19.39127049421167	25.36666764607941	34.61342578989638	20.62863606981254
15	18.378924996572053	23.64902254608137	36.23619028030793	21.735862177038648
16	22.511679496973613	23.744760141818965	33.097662141779786	20.64589821942763
17	22.11379306967542	24.224917239623124	31.411970382558618	22.249319308142837
18	24.187454702160586	22.68858592388004	31.320640144169552	21.80331922978982
19	22.13130007247654	25.713379757497407	29.36940510469922	22.78591506532683
20	24.06735421441304	25.471832089479147	29.272443243031475	21.188370453076335
21	23.644860041918868	26.13954672778202	30.618523633229515	19.5970695970696
22	23.12540400775695	22.259235862177036	30.747438835674128	23.867921294391884
23	21.244686685863158	25.891020743961917	30.529152220328694	22.335140349846235
24	21.753001900060724	23.798872695931518	32.1524064171123	22.29571898689546
25	23.02783001312413	25.600747291923764	29.595404595404595	21.77601809954751
26	22.232057158527745	24.180353959765725	29.6284597755186	23.95912910618793
27	23.612661847955966	22.603254588548708	30.087192219545162	23.69689134395017
28	21.771488315605964	24.71303206597324	30.442719045660223	23.072760572760572
29	23.368543221484398	24.87573700808995	28.87357740298917	22.882142367436483
30	25.68852715911539	22.450221347280173	30.22920706744236	21.63204442616207
31	25.764431646784587	23.984838690721045	28.43908542437954	21.811644238114827
32	23.82397014749956	25.628660555131145	29.11145227321698	21.435917024152317
33	23.213061448355564	24.285885682944507	28.105228105228104	24.395824763471822
34	22.25237997296821	25.17715128009246	30.300214491390964	22.270254255548373
35	21.94178370648959	26.82329924976984	29.54386299974535	21.69105404399522
36	22.847862921392334	26.822074983839688	28.02136588901295	22.30869620575503
37	22.042908072319836	26.52384380325557	27.901265401265402	23.531982723159196
38	20.93764079058197	26.784612446377153	28.790204893146072	23.487541869894812
39	21.990509490509492	25.069660731425436	27.269911461087933	25.669918316977142
40	23.86632974868269	23.507987110928287	29.941259720671486	22.684423419717536
41	20.86038961038961	25.166010460128106	29.014490411549236	24.959109517933047
42	22.520371785077668	25.18486415545239	29.37185363655952	22.922910422910423
43	22.761919453095924	23.378337348925584	29.631765293529998	24.22797790444849
44	23.261052672817378	23.682077726195374	28.69103935280406	24.36583024818319
45	23.213551154727625	22.98767409061527	28.262668703845172	25.536106050811934
46	23.23717948717949	23.539695598519128	27.907631584102173	25.315493330199214
47	22.07804450451509	24.5260866584396	30.048995122524534	23.346873714520775
48	21.488560459148694	24.510048774754654	30.187949305596362	23.813441460500286
49	23.348097980450923	22.927685060038	30.364978159095806	23.359238800415273
50	21.20367377720319	23.402700240935534	31.77557736381266	23.61804861804862
51	21.89513917455094	21.959535562476738	29.492688683865154	26.65263657910717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	314.0
1	708.0
2	1102.0
3	9803.0
4	18504.0
5	11571.0
6	4638.0
7	4505.5
8	4373.0
9	4413.5
10	4454.0
11	4355.5
12	4257.0
13	4168.0
14	4079.0
15	3805.5
16	3532.0
17	3543.0
18	3554.0
19	3325.0
20	3096.0
21	3061.5
22	3027.0
23	3314.0
24	3601.0
25	4124.5
26	5218.0
27	5788.0
28	6433.0
29	7078.0
30	8405.5
31	9733.0
32	11258.0
33	12783.0
34	14919.0
35	17055.0
36	18325.5
37	19596.0
38	21616.5
39	23637.0
40	25855.0
41	28073.0
42	31132.5
43	34192.0
44	39156.0
45	44120.0
46	56551.0
47	68982.0
48	73111.0
49	77240.0
50	76938.5
51	76637.0
52	69905.0
53	63173.0
54	57779.5
55	52386.0
56	48750.0
57	45114.0
58	42954.0
59	40794.0
60	37786.5
61	34779.0
62	31129.5
63	27480.0
64	24140.0
65	20800.0
66	17468.5
67	14137.0
68	12104.0
69	10071.0
70	8657.5
71	7244.0
72	6402.0
73	5560.0
74	4463.0
75	2686.0
76	2006.0
77	1501.0
78	996.0
79	759.5
80	523.0
81	351.0
82	179.0
83	116.5
84	54.0
85	44.5
86	35.0
87	25.0
88	15.0
89	9.5
90	4.0
91	3.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	816816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.19058680214055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0694538862347	27.523747344251127
2	9.531488265453943	7.280262599108058
3	4.003715808139727	4.587127683055877
4	2.408831765361031	3.679787945070957
5	1.7033589446521533	3.25261388154703
6	1.3462805431446825	3.0849146365799958
7	1.2098070113754107	3.234226777723961
8	1.0243204962181962	3.1295520659226166
9	0.9061976258684752	3.1147397181561325
>10	5.708055319816911	33.4069144826965
>50	0.054256878594608815	1.3865812117594292
>100	0.028743184032785363	2.149309260981563
>500	0.0019377427437797929	0.5314687022587194
>1k	0.0032295712396329885	2.209868818373051
>5k	0.0	0.0
>10k+	3.229571239632988E-4	1.42888487251503
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11585	1.418312080076786	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	3005	0.36789191200955906	RNA PCR Primer, Index 11 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	2593	0.3174521556874498	RNA PCR Primer, Index 11 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	2379	0.2912528647822766	TruSeq Adapter, Index 11 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	2015	0.24668958492487905	TruSeq Adapter, Index 11 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	1648	0.20175902528843706	RNA PCR Primer, Index 11 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1359	0.16637773990715166	No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1342	0.1642964878258996	Illumina PCR Primer Index 11 (100% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	1298	0.15890971773324714	TruSeq Adapter, Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1267	0.15511449334978747	RNA PCR Primer, Index 11 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	1011	0.12377328553799141	TruSeq Adapter, Index 11 (96% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.897063720593133E-4	0.0	0.0	0.13050674815380697	0.0
2	4.897063720593133E-4	0.0	0.0	0.5290053084170732	0.0
3	4.897063720593133E-4	0.0	0.0	0.990431137489961	0.0
4	4.897063720593133E-4	0.0	0.0	1.495930540048187	0.0
5	4.897063720593133E-4	0.0	0.0	2.858783373489256	0.0
6	4.897063720593133E-4	0.0	0.0	4.527335409688351	0.0
7	4.897063720593133E-4	0.0	0.0	5.63284754461225	0.0
8	4.897063720593133E-4	0.0	0.0	7.192072633249103	0.0
9	4.897063720593133E-4	0.0	0.0	8.10990480108127	0.0
10	6.121329650741415E-4	0.0	0.0	9.373836947366359	0.0
11	6.121329650741415E-4	0.0	0.0	10.484735852382912	0.0
12	6.121329650741415E-4	0.0	0.0	11.49842804254569	0.0
13	6.121329650741415E-4	0.0	0.0	12.023270846800258	0.0
14	6.121329650741415E-4	0.0	0.0	12.338152044034397	0.0
15	6.121329650741415E-4	0.0	0.0	12.641402714932127	0.0
16	6.121329650741415E-4	0.0	0.0	13.084831834831835	0.0
17	6.121329650741415E-4	0.0	0.0	13.604043015807722	0.0
18	6.121329650741415E-4	0.0	0.0	14.184589919884038	0.0
19	6.121329650741415E-4	0.0	0.0	14.577824136647665	0.0
20	6.121329650741415E-4	0.0	0.0	14.944002076355018	0.0
21	7.345595580889699E-4	0.0	0.0	15.350703218350278	0.0
22	8.569861511037982E-4	0.0	0.0	15.808578676225736	0.0
23	0.0011018393371334547	0.0	0.0	16.258863685334273	0.0
24	0.0011018393371334547	0.0	0.0	16.642058921470685	0.0
25	0.001224265930148283	0.0	0.0	16.982159996865878	0.0
26	0.001224265930148283	0.0	0.0	17.347970656794185	0.0
27	0.0013466925231631114	0.0	0.0	17.737776928953398	0.0
28	0.0013466925231631114	0.0	0.0	18.098812951754127	0.0
29	0.0013466925231631114	0.0	0.0	18.48274274744863	0.0
30	0.0013466925231631114	0.0	0.0	18.984446925623395	0.0
31	0.0013466925231631114	0.0	0.0	19.409144776791834	0.0
32	0.0013466925231631114	0.0	0.0	19.877181641887525	0.0
33	0.0013466925231631114	0.0	0.0	20.343504534681006	0.0
34	0.0013466925231631114	0.0	0.0	20.794401676754617	0.0
35	0.0013466925231631114	0.0	0.0	21.23746351687528	0.0
36	0.0014691191161779397	0.0	0.0	21.611354331942568	0.0
37	0.001591545709192768	0.0	0.0	21.98695911931206	0.0
38	0.0017139723022075964	0.0	0.0	22.393537834714305	0.0
39	0.0017139723022075964	0.0	0.0	22.927562633444985	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	20	7.032087E-4	45.0	24
TCGACGT	20	7.032087E-4	45.0	26
CGTTATT	590	0.0	43.474575	1
CGTAAGG	135	0.0	43.333336	2
CGTTTTT	4770	0.0	42.9717	1
CGCACGG	110	0.0	42.954544	2
TTACGGG	190	0.0	40.263157	3
TACGGGA	280	0.0	39.375	4
TCACGGG	115	0.0	39.130436	3
CGTTTCT	420	0.0	39.107143	1
GCGAGAC	540	0.0	37.916668	21
ACACGAC	525	0.0	37.714283	26
CCTTAAC	30	1.1396327E-4	37.499996	34
ATTCGCG	30	1.1396327E-4	37.499996	1
CACGACC	530	0.0	37.35849	27
AGTACGG	85	0.0	37.058823	2
AACACGT	465	0.0	36.774193	41
AGACACG	540	0.0	36.666668	24
ACGACCA	535	0.0	36.588783	28
TCAAGCG	560	0.0	36.5625	17
>>END_MODULE