SRR2936415.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936415.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689355
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	11481	1.665469895772135	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10391	1.5073510745552001	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	5104	0.7404022600837015	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	4788	0.6945623082446635	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	3352	0.4862516410267569	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2512	0.36439860449260536	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	2423	0.3514879851455346	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	1733	0.251394419421053	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	1604	0.2326812745247369	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	1482	0.21498357159953868	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	1268	0.1839400599110763	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	1220	0.17697702925198192	No Hit
GAATATGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	1179	0.17102944056400549	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1153	0.16725779895699602	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	1038	0.1505755380029158	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	871	0.1263499938348166	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	855	0.12402898361511848	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	832	0.12069253142430243	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT	757	0.10981279601946749	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAC	35	1.210974E-7	45.000004	17
TCGACGG	35	1.210974E-7	45.000004	2
CCCGTAA	20	7.03132E-4	45.0	41
ACTAACG	20	7.03132E-4	45.0	1
CGTAAGG	85	0.0	45.0	2
CGGTCTA	25	3.8891754E-5	45.0	31
CGTTTTT	3995	0.0	43.535667	1
CCGATGA	1045	0.0	42.416267	18
CGATGAA	1085	0.0	41.682026	19
CGTTATT	510	0.0	41.47059	1
ACACGAC	95	0.0	40.263157	26
TGTCGAA	45	1.9263098E-8	40.000004	42
TTTAGCG	40	3.4555342E-7	39.375	1
ACCCGTC	40	3.4555342E-7	39.375	39
ACCGATG	1130	0.0	39.225666	17
ATGAATG	1215	0.0	39.074074	21
GCTACGA	75	0.0	39.0	10
CCCGTCG	70	0.0	38.57143	40
CCCCGTA	35	6.2446506E-6	38.57143	40
ATAGGGC	135	0.0	38.333336	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content