##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936413.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232867
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912593884062577	33.0	31.0	34.0	30.0	34.0
2	32.00754507938007	33.0	31.0	34.0	30.0	34.0
3	31.989891225463463	33.0	31.0	34.0	30.0	34.0
4	35.65163805949319	37.0	35.0	37.0	33.0	37.0
5	31.843344054760873	37.0	35.0	37.0	0.0	37.0
6	33.70150343329025	37.0	35.0	37.0	17.0	37.0
7	35.410109633395884	37.0	35.0	37.0	32.0	37.0
8	35.6078405269961	37.0	35.0	37.0	33.0	37.0
9	37.55658809535056	39.0	37.0	39.0	35.0	39.0
10	37.20867276170518	39.0	37.0	39.0	34.0	39.0
11	37.16679477985288	39.0	37.0	39.0	34.0	39.0
12	36.9564042994499	39.0	35.0	39.0	33.0	39.0
13	36.68970270583638	39.0	35.0	39.0	33.0	39.0
14	37.82087629419368	40.0	36.0	41.0	33.0	41.0
15	37.95496141574375	40.0	36.0	41.0	33.0	41.0
16	38.01967646768327	40.0	36.0	41.0	34.0	41.0
17	38.02091751944243	40.0	36.0	41.0	34.0	41.0
18	37.98130263197447	40.0	36.0	41.0	34.0	41.0
19	37.873455663533264	40.0	36.0	41.0	34.0	41.0
20	37.891903962347605	40.0	36.0	41.0	34.0	41.0
21	37.8862569621286	40.0	36.0	41.0	34.0	41.0
22	37.96010598324366	40.0	36.0	41.0	34.0	41.0
23	37.95900234898032	40.0	36.0	41.0	34.0	41.0
24	37.92789446336321	40.0	35.0	41.0	34.0	41.0
25	37.737846066638895	40.0	35.0	41.0	33.0	41.0
26	37.75707592746074	40.0	35.0	41.0	33.0	41.0
27	37.688998441170284	40.0	35.0	41.0	33.0	41.0
28	37.6738567508492	40.0	35.0	41.0	33.0	41.0
29	37.66433629496665	40.0	36.0	41.0	33.0	41.0
30	37.50838890869037	40.0	36.0	41.0	33.0	41.0
31	37.53852628324322	40.0	35.0	41.0	33.0	41.0
32	37.41150527983785	40.0	35.0	41.0	33.0	41.0
33	37.377704870161935	40.0	35.0	41.0	33.0	41.0
34	37.267633456007076	40.0	35.0	41.0	33.0	41.0
35	37.250825578549126	40.0	35.0	41.0	33.0	41.0
36	37.17002838530148	39.0	35.0	41.0	33.0	41.0
37	37.03450467434201	39.0	35.0	41.0	32.0	41.0
38	36.97037364675974	39.0	35.0	41.0	32.0	41.0
39	36.96200835670147	39.0	35.0	41.0	32.0	41.0
40	36.839908617365275	39.0	35.0	41.0	31.0	41.0
41	36.80092069722202	39.0	35.0	41.0	31.0	41.0
42	36.75023940704351	39.0	35.0	41.0	32.0	41.0
43	36.66079350015245	39.0	35.0	41.0	31.0	41.0
44	36.610850829013984	39.0	35.0	41.0	31.0	41.0
45	36.65826415936994	39.0	35.0	41.0	31.0	41.0
46	36.56387551692597	39.0	35.0	41.0	31.0	41.0
47	36.38414202098193	39.0	35.0	41.0	31.0	41.0
48	36.38820442570223	38.0	35.0	40.0	31.0	41.0
49	36.38057346038726	38.0	35.0	40.0	31.0	41.0
50	36.243044312848106	38.0	35.0	40.0	31.0	41.0
51	35.16562243684163	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	5.0
11	8.0
12	5.0
13	3.0
14	2.0
15	9.0
16	16.0
17	17.0
18	52.0
19	100.0
20	231.0
21	386.0
22	635.0
23	800.0
24	1019.0
25	1275.0
26	1558.0
27	1806.0
28	2147.0
29	2698.0
30	3372.0
31	4588.0
32	6251.0
33	9741.0
34	16728.0
35	23660.0
36	17148.0
37	26189.0
38	43640.0
39	68730.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.24585278291901	19.137533441835895	22.23415082429026	11.382462950954837
2	31.652402444313704	27.512270952947393	23.225703942593842	17.60962266014506
3	30.894888498585026	26.779234498662323	25.978348155814263	16.34752884693838
4	27.893604503858427	24.064380096793446	29.373419162010933	18.668596237337194
5	24.505404372452947	36.053627177745234	20.97635130782807	18.464617141973743
6	23.421094444468302	36.42293669777169	24.68619426539613	15.46977459236388
7	75.81881503175633	5.776258551018393	13.470779457802093	4.93414695942319
8	74.95222594871751	8.866863918030464	9.703392923857825	6.477517209394204
9	69.71618992815641	6.916394336681453	11.718706386048689	11.648709349113442
10	41.90074162504777	22.78811510432994	19.59530547479892	15.715837795823367
11	33.32975475271292	23.68734084262691	24.148548312985525	18.834356091674646
12	29.859533553487612	19.79713742179012	29.2600497279563	21.083279296765962
13	23.619920383738357	27.157991471526664	32.532733276934906	16.689354867800073
14	19.928113472497177	31.34235421936127	27.648829589422284	21.08070271871927
15	16.95087753953974	25.094152456123027	39.54317271232077	18.411797292016473
16	18.234013406794435	23.932974616411943	33.18847238981908	24.64453958697454
17	18.978644462289633	23.220121356826	29.33262334293824	28.468610837946123
18	20.236014549077368	23.717400919838365	32.93510888189395	23.11147564919031
19	21.172171239376983	26.349804824212963	27.85624412218133	24.621779814228724
20	23.940704350552032	25.726272936912487	30.227554784490717	20.105467928044764
21	21.51915041633207	29.75561157227087	28.344505662030254	20.380732349366806
22	19.118209106485676	24.59086087766837	29.227842502372596	27.063087513473356
23	18.40621470624863	28.84221465471707	28.773505906805173	23.978064732229125
24	21.94342693468804	24.09572846302825	30.058788922432118	23.90205567985159
25	18.16530465888254	28.31015128807431	28.86798043518403	24.65656361785912
26	19.45488197125398	26.393606651006795	29.862539561208756	24.288971816530466
27	20.57268741384567	28.603022326048777	28.028874851309975	22.795415408795577
28	16.436850219223846	26.296555544581242	32.93940317863845	24.327191057556462
29	22.13924686623695	23.572683119548927	30.384296615664734	23.903773398549387
30	20.110621084138156	29.028157703753642	30.083695843550178	20.777525368558017
31	24.94685807778689	25.544194754945952	23.81960518235731	25.68934198490984
32	24.310872729927382	31.7567538552049	23.317172463251556	20.61520095161616
33	23.303430713669176	26.429249313986098	26.47949258589667	23.787827386448058
34	23.858683282732205	24.646686735346787	27.396754370520508	24.0978756114005
35	20.6547084816655	28.57940369395406	26.56838452850769	24.197503295872753
36	27.065234661845604	25.849089823805006	26.163432345501942	20.922243168847455
37	21.41737558348757	29.231277939768198	28.380148325009554	20.971198151734683
38	19.364701739619612	29.768494462504346	25.148260595103643	25.7185432027724
39	23.815310885612817	26.08184070735656	28.08985386508179	22.01299454194884
40	23.216685919430404	25.076545839470597	29.83935035878849	21.8674178823105
41	22.863694727033028	25.526588138293533	25.724125788540242	25.885591346133204
42	22.602601484967813	26.150549455268457	27.29154410028042	23.95530495948331
43	24.822752901871027	24.597302322785108	25.971906710697525	24.608038064646344
44	21.179471543842624	24.63036840771771	28.529160422043482	25.660999626396187
45	20.8565404286567	25.97319499972087	26.20293987555128	26.96732469607115
46	25.27408348971731	26.567525669158794	27.28510265516368	20.873288185960227
47	20.716975784460658	24.418659578214175	31.827180321814595	23.03718431551057
48	21.760060463698164	25.186909265804086	28.08598899801174	24.967041272486014
49	21.711534910485383	21.232291393799894	33.148535430095286	23.90763826561943
50	21.732576964533404	22.555364220778383	30.217677901978384	25.49438091270983
51	20.125221693069435	23.24588713729296	26.81187115392048	29.817020015717127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	192.0
2	281.0
3	583.0
4	885.0
5	588.5
6	292.0
7	304.5
8	317.0
9	333.0
10	349.0
11	348.5
12	348.0
13	376.0
14	404.0
15	381.0
16	358.0
17	383.5
18	409.0
19	471.5
20	534.0
21	561.5
22	589.0
23	685.0
24	781.0
25	848.5
26	1337.0
27	1758.0
28	1757.0
29	1756.0
30	2392.0
31	3028.0
32	3510.0
33	3992.0
34	4615.0
35	5238.0
36	5427.5
37	5617.0
38	6210.5
39	6804.0
40	8276.0
41	9748.0
42	12025.0
43	14302.0
44	16905.0
45	19508.0
46	25387.5
47	31267.0
48	29557.5
49	27848.0
50	27367.0
51	26886.0
52	22862.5
53	18839.0
54	15999.0
55	13159.0
56	11385.5
57	9612.0
58	8309.5
59	7007.0
60	6618.0
61	6229.0
62	5619.5
63	5010.0
64	4204.0
65	3398.0
66	2712.0
67	2026.0
68	1643.5
69	1261.0
70	1022.0
71	783.0
72	681.0
73	579.0
74	433.5
75	258.0
76	228.0
77	135.5
78	43.0
79	34.5
80	26.0
81	22.5
82	19.0
83	14.0
84	9.0
85	19.0
86	29.0
87	15.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	232867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.54875959238535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95264951239572	26.663288486560997
2	11.30889437198919	8.266521233150254
3	4.0876512748208205	4.481957512228011
4	2.112560216190812	3.0884582186398246
5	1.2571965691458111	2.2974487583040966
6	0.8718129479497121	1.9118209106485675
7	0.6826459875455293	1.7464904859855623
8	0.5428269298554811	1.5871720767648485
9	0.46763012571965695	1.5382170938776212
>10	5.604511808248149	39.36152396002869
>50	0.07167195394195747	1.8018869139895304
>100	0.031723651744800845	2.5160284625988223
>500	0.002349900129244507	0.474519790266547
>1k	0.0058747503231112675	4.264666096956632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	2564	1.1010576852881688	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	2360	1.0134540317004985	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	2326	0.9988534227692202	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1680	0.7214418530749311	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1001	0.4298591041238132	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	565	0.24262776606389058	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	540	0.23189202420265645	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.21084997015463763	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	396	0.17005415108194805	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	369	0.15845954987181524	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	356	0.1528769641039735	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	350	0.15030038605727733	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	338	0.14514722996388496	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	332	0.1425706519171888	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	295	0.12668175396256232	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	279	0.1198108791713725	No Hit
ACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	240	0.1030631218678473	No Hit
GAATATGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	233	0.10005711414670176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.588593488987276E-4	0.0	0.0	0.25679894532071956	0.0
2	8.588593488987276E-4	0.0	0.0	1.7370430331476765	0.0
3	8.588593488987276E-4	0.0	0.0	2.1969622144829453	0.0
4	8.588593488987276E-4	0.0	0.0	2.941163840303693	0.0
5	8.588593488987276E-4	0.0	0.0	5.531054206907806	0.0
6	8.588593488987276E-4	0.0	0.0	6.515736450420197	0.0
7	8.588593488987276E-4	0.0	0.0	7.725439843344055	0.0
8	8.588593488987276E-4	0.0	0.0	9.390768120858688	0.0
9	8.588593488987276E-4	0.0	0.0	9.967921603318633	0.0
10	8.588593488987276E-4	0.0	0.0	12.404505576144322	0.0
11	8.588593488987276E-4	0.0	0.0	14.224428536460726	0.0
12	8.588593488987276E-4	0.0	0.0	16.88775137739568	0.0
13	8.588593488987276E-4	0.0	0.0	17.526313303301883	0.0
14	8.588593488987276E-4	0.0	0.0	17.772376506761372	0.0
15	8.588593488987276E-4	0.0	0.0	18.403208698527486	0.0
16	8.588593488987276E-4	0.0	0.0	19.391326379435473	0.0
17	8.588593488987276E-4	0.0	0.0	20.55508079719325	0.0
18	8.588593488987276E-4	0.0	0.0	21.926679177384514	0.0
19	8.588593488987276E-4	0.0	0.0	22.791979971399982	0.0
20	8.588593488987276E-4	0.0	0.0	23.501827223264783	0.0
21	8.588593488987276E-4	0.0	0.0	24.329338205928707	0.0
22	8.588593488987276E-4	0.0	0.0	25.125071392683378	0.0
23	8.588593488987276E-4	0.0	0.0	26.023008841956997	0.0
24	0.0017177186977974553	0.0	0.0	26.652123315025314	0.0
25	0.0017177186977974553	0.0	0.0	27.253754288928874	0.0
26	0.0017177186977974553	0.0	0.0	27.810724576689697	0.0
27	0.0017177186977974553	0.0	0.0	28.38873691849854	0.0
28	0.0017177186977974553	0.0	0.0	28.944418917236018	0.0
29	0.0017177186977974553	0.0	0.0	29.48206486964662	0.0
30	0.0017177186977974553	0.0	0.0	30.15326345081098	0.0
31	0.0017177186977974553	0.0	0.0	30.723546058479734	0.0
32	0.0017177186977974553	0.0	0.0	31.272786612100468	0.0
33	0.0017177186977974553	0.0	0.0	31.863252414468345	0.0
34	0.0017177186977974553	0.0	0.0	32.42709357702036	0.0
35	0.0017177186977974553	0.0	0.0	33.059214057809825	0.0
36	0.0017177186977974553	0.0	0.0	33.57452966714906	0.0
37	0.0017177186977974553	0.0	0.0	34.0855509797438	0.0
38	0.0017177186977974553	0.0	0.0	34.591848565919605	0.0
39	0.0017177186977974553	0.0	0.0	35.1372242524703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.021689E-4	45.000004	19
AACGTCA	20	7.021689E-4	45.000004	44
GCGACGC	20	7.021689E-4	45.000004	23
CCGATGG	20	7.021689E-4	45.000004	10
ACTGAAC	30	2.1580236E-6	45.000004	16
ACACCGA	20	7.021689E-4	45.000004	44
GTCTCGC	20	7.021689E-4	45.000004	31
CACGCGA	20	7.021689E-4	45.000004	20
CGCGACG	20	7.021689E-4	45.000004	22
TACGGAG	20	7.021689E-4	45.000004	1
CGTTATT	30	2.1580236E-6	45.000004	1
TGCGATG	20	7.021689E-4	45.000004	1
CGAATGC	20	7.021689E-4	45.000004	45
CGCAGTA	20	7.021689E-4	45.000004	37
AATTCGG	20	7.021689E-4	45.000004	13
ACGTCAC	20	7.021689E-4	45.000004	45
CGTAAGG	60	0.0	45.000004	2
GGTTGCG	30	2.1580236E-6	45.000004	1
ATCGGGG	65	0.0	45.000004	4
CAGTAAC	20	7.021689E-4	45.000004	39
>>END_MODULE