##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557663
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00759239899366	33.0	31.0	34.0	30.0	34.0
2	32.092086080661616	33.0	31.0	34.0	30.0	34.0
3	32.06776852687017	33.0	31.0	34.0	30.0	34.0
4	35.69389577576422	37.0	35.0	37.0	33.0	37.0
5	32.09722717842138	37.0	35.0	37.0	0.0	37.0
6	33.84716934779607	37.0	35.0	37.0	17.0	37.0
7	35.447702286147724	37.0	35.0	37.0	32.0	37.0
8	35.68483116147207	37.0	35.0	37.0	33.0	37.0
9	37.56693020695295	39.0	37.0	39.0	35.0	39.0
10	37.231105524304105	39.0	37.0	39.0	34.0	39.0
11	37.25263644889476	39.0	37.0	39.0	34.0	39.0
12	37.183374188353895	39.0	37.0	39.0	34.0	39.0
13	37.12255430250886	39.0	37.0	39.0	33.0	39.0
14	38.306769500576515	40.0	38.0	41.0	33.0	41.0
15	38.44095986285624	40.0	38.0	41.0	34.0	41.0
16	38.37701443344816	40.0	38.0	41.0	34.0	41.0
17	38.35381942140683	40.0	37.0	41.0	34.0	41.0
18	38.266856506528136	40.0	37.0	41.0	34.0	41.0
19	38.15437459540977	40.0	37.0	41.0	34.0	41.0
20	38.2526884516276	40.0	37.0	41.0	34.0	41.0
21	38.15347441017245	40.0	37.0	41.0	34.0	41.0
22	38.230944136512555	40.0	37.0	41.0	34.0	41.0
23	38.24041939307431	40.0	37.0	41.0	34.0	41.0
24	38.217941660106554	40.0	37.0	41.0	34.0	41.0
25	37.92543525390783	40.0	36.0	41.0	33.0	41.0
26	37.97717797307693	40.0	37.0	41.0	34.0	41.0
27	37.93820461461492	40.0	37.0	41.0	33.0	41.0
28	37.78626159526453	40.0	36.0	41.0	33.0	41.0
29	37.69866747480109	40.0	36.0	41.0	33.0	41.0
30	37.56665226131194	40.0	36.0	41.0	33.0	41.0
31	37.59901409991339	40.0	36.0	41.0	33.0	41.0
32	37.55614053648888	40.0	36.0	41.0	33.0	41.0
33	37.494626683140176	40.0	36.0	41.0	33.0	41.0
34	37.4025800528276	40.0	36.0	41.0	33.0	41.0
35	37.397483785009946	40.0	36.0	41.0	33.0	41.0
36	37.288590062457075	40.0	35.0	41.0	32.0	41.0
37	37.26460066384178	40.0	35.0	41.0	32.0	41.0
38	37.12495898060298	39.0	35.0	41.0	32.0	41.0
39	37.02335639983287	39.0	35.0	41.0	32.0	41.0
40	36.94864999112367	39.0	35.0	41.0	31.0	41.0
41	36.80894195957057	39.0	35.0	41.0	31.0	41.0
42	36.78074033959578	39.0	35.0	41.0	31.0	41.0
43	36.683529299953555	39.0	35.0	41.0	31.0	41.0
44	36.61586836494442	39.0	35.0	41.0	31.0	41.0
45	36.574791585599186	39.0	35.0	41.0	31.0	41.0
46	36.41063868321908	39.0	35.0	41.0	31.0	41.0
47	36.31844142430106	38.0	35.0	41.0	31.0	41.0
48	36.280490188518876	38.0	35.0	40.0	31.0	41.0
49	36.199385650473495	38.0	35.0	40.0	31.0	41.0
50	36.0598210747351	38.0	35.0	40.0	30.0	41.0
51	35.01022660639131	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	33.0
10	21.0
11	27.0
12	18.0
13	13.0
14	14.0
15	24.0
16	40.0
17	82.0
18	157.0
19	250.0
20	470.0
21	816.0
22	1271.0
23	1808.0
24	2331.0
25	3183.0
26	4067.0
27	4828.0
28	5356.0
29	6592.0
30	8088.0
31	10528.0
32	13781.0
33	20284.0
34	33465.0
35	44239.0
36	45974.0
37	70623.0
38	110919.0
39	168225.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.43431606543737	18.539153574829243	22.600746328876042	12.425784030857345
2	32.16655937367191	23.985274260619764	24.546365815913912	19.30180054979441
3	32.91306756948193	25.05796511513226	24.8571269745348	17.171840340851016
4	27.92654345007648	25.30004680245955	25.867235229878975	20.906174517584994
5	22.755319969228726	36.84286029376164	21.785737981540823	18.616081755468805
6	24.60733453716671	35.0510971680029	24.375832716174465	15.965735578655927
7	79.44798202498642	6.088444096165605	9.572985835531494	4.890588043316483
8	79.52795864168863	6.123949410306942	8.937117936818472	5.410974011185967
9	73.52002912153038	7.6062066158235355	11.016689290844111	7.857074971801967
10	38.212863324265726	27.381769993705873	17.357256981366884	17.04810970066151
11	29.29385668405471	24.938717469152515	24.546365815913912	21.221060030878864
12	25.710509752305605	21.285256507962693	31.064101437606585	21.940132302125118
13	24.99089952175418	23.84289436451764	31.23786229317706	19.928343820551124
14	20.717171481701314	28.20484773061867	30.222195125012774	20.85578566266724
15	19.059001583393556	24.030821481790973	34.70985164875561	22.20032528605986
16	21.553698201243403	24.860175410597442	30.306834055693134	23.279292332466024
17	22.53690849132899	23.09817936639153	27.863960850908164	26.50095129137131
18	25.52096875711675	22.47880888637044	29.17299516015945	22.827227196353352
19	24.13823402305694	24.950911213403078	26.225695446891763	24.685159316648225
20	25.766636839811856	24.04821549932486	26.68439541443488	23.500752246428398
21	25.317799459530217	26.472080808660426	27.386073668147247	20.824046063662106
22	23.65353268909718	23.654787927475912	26.736398147268154	25.955281236158754
23	21.81263594679941	26.595811448849933	26.709500182009567	24.88205242234109
24	22.822385562606804	23.29901750698899	28.40084423746958	25.477752692934626
25	22.0262057909526	26.94835411350583	26.080087794958608	24.945352300582968
26	22.05346239574797	25.179185278564294	26.562637291697673	26.204715033990063
27	23.509718234847927	24.75581130539412	25.888753602085846	25.84571685767211
28	20.29702526436217	25.632147013518917	27.815006554137533	26.25582116798138
29	21.118130483822668	26.108599638132706	27.562703640011975	25.210566238032644
30	22.513776241206607	24.510860501772576	27.750451437516922	25.22491181950389
31	23.717370526644228	25.189047865825774	24.01432406309904	27.079257544430956
32	22.61276075335821	26.32091424390716	25.456951599801315	25.60937340293331
33	22.030509465393973	23.86746117278715	26.043865201743703	28.058164160075172
34	21.080473332460645	24.873624393226734	27.475733552342547	26.570168721970077
35	20.67879705126573	25.414094175155967	27.62654147755903	26.28056729601928
36	21.884722493692426	27.63174175084235	26.486067750594895	23.997468004870328
37	20.80163109261328	25.943266811676587	25.85844856122784	27.396653534482297
38	20.172756664867492	26.83538265941976	26.279312057640546	26.71254861807221
39	21.837561394605704	24.2189279188327	26.42904406424669	27.51446662231491
40	23.804878573618833	22.49907202019858	29.272158992079444	24.42389041410314
41	19.781660250007622	25.30327455829058	26.844169328070894	28.070895863630902
42	22.33158735652177	25.02801871381103	27.613271814698127	25.027122114969075
43	22.228119850160404	24.571291263720205	26.49754421577189	26.703044670347502
44	21.755253620914424	23.345461327002152	27.116556056256197	27.782728995827227
45	21.976175575571627	22.146350035774294	27.22002356261757	28.657450826036513
46	23.03039649393989	23.778159928128638	27.42086170321502	25.770581874716452
47	20.279810566596673	24.877210788594546	29.17227788108589	25.67070076372289
48	21.00372447158947	24.477686344620317	27.78577743188987	26.732811751900343
49	22.5367291715606	23.118083860682887	29.396965550879294	24.948221416877217
50	20.89308417449248	23.438169647260086	30.087884618488225	25.58086155975921
51	21.158477431710548	22.493513107378472	27.062580805970633	29.285428654940347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197.0
1	527.0
2	857.0
3	1534.5
4	2212.0
5	1481.5
6	751.0
7	776.0
8	801.0
9	842.5
10	884.0
11	826.0
12	768.0
13	791.0
14	814.0
15	830.5
16	847.0
17	921.0
18	995.0
19	994.0
20	993.0
21	1000.5
22	1008.0
23	1474.0
24	1940.0
25	2231.5
26	3103.5
27	3684.0
28	4211.5
29	4739.0
30	5808.5
31	6878.0
32	7566.5
33	8255.0
34	10193.5
35	12132.0
36	12813.0
37	13494.0
38	14982.5
39	16471.0
40	18317.5
41	20164.0
42	23634.0
43	27104.0
44	31010.0
45	34916.0
46	42313.5
47	49711.0
48	51619.0
49	53527.0
50	53788.5
51	54050.0
52	48873.5
53	43697.0
54	40542.0
55	37387.0
56	34592.5
57	31798.0
58	29841.0
59	27884.0
60	26173.5
61	24463.0
62	22722.0
63	20981.0
64	18627.0
65	16273.0
66	13813.0
67	11353.0
68	9905.0
69	8457.0
70	7045.5
71	5634.0
72	4686.5
73	3739.0
74	3104.5
75	1988.0
76	1506.0
77	1188.0
78	870.0
79	559.5
80	249.0
81	162.5
82	76.0
83	70.5
84	65.0
85	44.0
86	23.0
87	16.0
88	9.0
89	8.5
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.78708288643975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8006370620959	26.05322432726844
2	10.087307240175287	7.219906006102736
3	3.7989444200517126	4.078594165241053
4	2.0523594397097313	2.9379182952663676
5	1.2908376030209163	2.309765614612137
6	0.9780943112657998	2.1001885312814625
7	0.7646042293645463	1.9154068452113986
8	0.7082515532531547	2.0277005632576275
9	0.6260781206679981	2.0164958637958867
>10	6.778715481716439	43.19534748459212
>50	0.07002459705616844	1.6926233945913294
>100	0.040086544691523875	2.672314759659011
>500	0.0010148492326968069	0.24133639002356794
>1k	0.003044547698090421	1.5391777590968863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1843	0.3304863331438521	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	1739	0.31183707723123105	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	1415	0.25373747227268084	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	1250	0.22414971048823393	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.2008381405974576	No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1109	0.19886562314516115	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	821	0.14722152984867204	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7931976839058716E-4	0.0	0.0	0.15511159965785787	0.0
2	1.7931976839058716E-4	0.0	0.0	0.8634246848006771	0.0
3	1.7931976839058716E-4	0.0	0.0	1.1766963201790328	0.0
4	1.7931976839058716E-4	0.0	0.0	1.6196161481037832	0.0
5	1.7931976839058716E-4	0.0	0.0	3.041621911441139	0.0
6	1.7931976839058716E-4	0.0	0.0	4.062489352888752	0.0
7	1.7931976839058716E-4	0.0	0.0	4.871580147867081	0.0
8	1.7931976839058716E-4	0.0	0.0	6.206615823535002	0.0
9	1.7931976839058716E-4	0.0	0.0	6.689703279579244	0.0
10	1.7931976839058716E-4	0.0	0.0	7.969149827046083	0.0
11	1.7931976839058716E-4	0.0	0.0	9.31530332835422	0.0
12	1.7931976839058716E-4	0.0	0.0	10.687278876310604	0.0
13	1.7931976839058716E-4	0.0	0.0	11.28459302481965	0.0
14	1.7931976839058716E-4	0.0	0.0	11.544068729680829	0.0
15	1.7931976839058716E-4	0.0	0.0	11.962780388872849	0.0
16	1.7931976839058716E-4	0.0	0.0	12.710005863756427	0.0
17	1.7931976839058716E-4	0.0	0.0	13.563926600832403	0.0
18	1.7931976839058716E-4	0.0	0.0	14.54982668744385	0.0
19	1.7931976839058716E-4	0.0	0.0	15.099441777561	0.0
20	3.586395367811743E-4	0.0	0.0	15.62466937917703	0.0
21	3.586395367811743E-4	0.0	0.0	16.25336448715443	0.0
22	3.586395367811743E-4	0.0	0.0	16.88044571721631	0.0
23	3.586395367811743E-4	0.0	0.0	17.49694708094315	0.0
24	3.586395367811743E-4	0.0	0.0	18.018946926728148	0.0
25	3.586395367811743E-4	0.0	0.0	18.47746757450288	0.0
26	5.379593051717614E-4	0.0	0.0	18.931146588531067	0.0
27	5.379593051717614E-4	0.0	0.0	19.37854941066558	0.0
28	7.172790735623486E-4	0.0	0.0	19.818958761832864	0.0
29	8.965988419529357E-4	0.0	0.0	20.26385110720991	0.0
30	8.965988419529357E-4	0.0	0.0	20.867082808075846	0.0
31	8.965988419529357E-4	0.0	0.0	21.33223828728103	0.0
32	8.965988419529357E-4	0.0	0.0	21.822319214292502	0.0
33	0.0010759186103435228	0.0	0.0	22.333739193742456	0.0
34	0.0010759186103435228	0.0	0.0	22.84175209759299	0.0
35	0.0010759186103435228	0.0	0.0	23.377380245775676	0.0
36	0.0010759186103435228	0.0	0.0	23.820300073700423	0.0
37	0.0010759186103435228	0.0	0.0	24.266806296992986	0.0
38	0.0010759186103435228	0.0	0.0	24.721561229631515	0.0
39	0.0010759186103435228	0.0	0.0	25.243202435879734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0301583E-4	45.000004	1
ATTACGG	50	2.1827873E-11	45.000004	2
TAAACGG	20	7.0301583E-4	45.000004	2
CGGGCTA	25	3.8882128E-5	45.000004	6
TTAACGG	40	6.8048394E-9	45.000004	2
ACGTAAG	30	2.1634842E-6	45.000004	1
GCTAACG	30	2.1634842E-6	45.000004	1
CCGACGG	35	1.2104283E-7	45.0	2
ACGGGCC	75	0.0	42.0	5
AACGGGC	130	0.0	41.538464	4
CCGGTAT	55	6.002665E-11	40.909092	42
TAACGGG	150	0.0	40.5	3
GCGAGAC	190	0.0	40.26316	21
TCAAGCG	190	0.0	40.26316	17
ACGTTAG	45	1.9252184E-8	40.000004	1
CGCGAGG	80	0.0	39.375004	2
AAACGGG	110	0.0	38.86364	3
TATACGG	70	0.0	38.571426	2
CGTAAGG	100	0.0	38.250004	2
TAATACG	65	9.094947E-12	38.076927	4
>>END_MODULE