FastQCFastQC Report
Sat 14 Jan 2017
SRR2936409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781202
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137921.7654844713659208No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC131611.6847115086750928No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG49100.6285186161837784No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC47460.6075253263560513No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT47080.6026610274935292No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC31850.40770504939823504No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT31390.4018166876172872No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC24010.30734688339251565No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG20550.2630561621706038No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT19490.2494873285014631No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT18840.24116681728925424No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.197260119661752No Hit
CGTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT14090.18036308150772784No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT13710.17549878264520571No Hit
GAATATGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC12370.1583457287615751No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC12290.1573216658431494No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC11880.15207334338621764No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT8990.11507907045808893Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC8370.1071425828402897No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT8130.10407039408501259No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG207.031899E-445.01
CAGACGA207.031899E-445.012
CCCGTAA302.1646065E-644.99999641
CGTTTTT66700.044.1904031
TAATACG2400.044.0624964
CGTTATT7600.042.631581
CGTTCTG2650.041.6037751
TACGGCT7600.041.1513147
TCGACGG501.0804797E-940.52
ATCGAAT501.0804797E-940.543
ACGGGAG4750.040.2631575
CCGATGA7500.040.218
CGACGGT403.4563345E-739.37528
CGATGAA8000.039.0937519
AATACGG2650.039.0566025
AGGTACG356.24572E-638.5714261
CCGTAAG356.24572E-638.57142642
ATCCCCG356.24572E-638.57142638
GTGTACG356.24572E-638.5714261
GCGTATG356.24572E-638.5714261
Produced by FastQC (version 0.11.5)