##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936409.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781202
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953187523841464	33.0	31.0	34.0	30.0	34.0
2	32.08552333455368	33.0	31.0	34.0	30.0	34.0
3	31.9410971810108	33.0	31.0	34.0	30.0	34.0
4	35.671818556532116	37.0	35.0	37.0	33.0	37.0
5	32.04860202610848	37.0	35.0	37.0	0.0	37.0
6	33.79800102918323	37.0	35.0	37.0	17.0	37.0
7	35.42663741260263	37.0	35.0	37.0	32.0	37.0
8	35.635792023061896	37.0	35.0	37.0	33.0	37.0
9	37.58604048632748	39.0	37.0	39.0	35.0	39.0
10	37.29513365301164	39.0	37.0	39.0	34.0	39.0
11	37.267176735338616	39.0	37.0	39.0	34.0	39.0
12	37.25917624378842	39.0	37.0	39.0	34.0	39.0
13	37.21764793228896	39.0	37.0	39.0	34.0	39.0
14	38.45669365925843	40.0	38.0	41.0	34.0	41.0
15	38.51859698259861	40.0	38.0	41.0	34.0	41.0
16	38.4946991943185	40.0	38.0	41.0	34.0	41.0
17	38.419959242295846	40.0	38.0	41.0	34.0	41.0
18	38.23456289154406	40.0	38.0	41.0	34.0	41.0
19	37.98252820653301	40.0	37.0	41.0	34.0	41.0
20	37.84366783495178	40.0	35.0	41.0	33.0	41.0
21	37.832757724634604	40.0	35.0	41.0	33.0	41.0
22	37.89280365385649	40.0	35.0	41.0	34.0	41.0
23	37.872606572947845	40.0	35.0	41.0	34.0	41.0
24	37.84180660059754	40.0	35.0	41.0	34.0	41.0
25	37.63563073315225	40.0	35.0	41.0	33.0	41.0
26	37.60748564391796	40.0	35.0	41.0	33.0	41.0
27	37.586621642033684	40.0	35.0	41.0	33.0	41.0
28	37.48765620159703	40.0	35.0	41.0	33.0	41.0
29	37.34112047844219	39.0	35.0	41.0	33.0	41.0
30	37.1255449934844	39.0	35.0	41.0	32.0	41.0
31	36.98866234341438	39.0	35.0	41.0	32.0	41.0
32	36.719765182372804	39.0	35.0	41.0	31.0	41.0
33	36.40480951149639	39.0	35.0	41.0	30.0	41.0
34	36.00938169641143	39.0	35.0	41.0	27.0	41.0
35	35.851559519816895	39.0	35.0	41.0	26.0	41.0
36	35.55569622197588	39.0	35.0	41.0	25.0	41.0
37	35.47151953015993	39.0	35.0	41.0	24.0	41.0
38	35.37265649601512	39.0	35.0	41.0	24.0	41.0
39	35.239397748597675	39.0	35.0	41.0	23.0	41.0
40	35.192470065360816	39.0	35.0	41.0	23.0	41.0
41	35.02861871833405	38.0	35.0	41.0	23.0	41.0
42	35.053173186960606	39.0	35.0	41.0	23.0	41.0
43	34.94855747937153	38.0	35.0	41.0	22.0	41.0
44	34.98447520615667	38.0	35.0	41.0	22.0	41.0
45	34.97939201384533	38.0	35.0	40.0	23.0	41.0
46	34.83576334930018	38.0	35.0	40.0	22.0	41.0
47	34.684134193204834	38.0	34.0	40.0	22.0	41.0
48	34.68480111418045	38.0	34.0	40.0	22.0	41.0
49	34.670640884175924	38.0	34.0	40.0	22.0	41.0
50	34.54184833116147	38.0	34.0	40.0	21.0	41.0
51	33.6073614762891	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	20.0
10	20.0
11	26.0
12	22.0
13	15.0
14	21.0
15	27.0
16	79.0
17	136.0
18	279.0
19	605.0
20	1172.0
21	2088.0
22	3400.0
23	4964.0
24	7229.0
25	10737.0
26	14232.0
27	15076.0
28	14143.0
29	13272.0
30	14005.0
31	16152.0
32	20741.0
33	28116.0
34	44576.0
35	55883.0
36	62324.0
37	91270.0
38	146221.0
39	214209.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.197403488470336	16.290792906316163	18.975757870563566	20.53604573464994
2	38.73313176361556	25.473437087974688	20.35081835427969	15.44261279413007
3	28.311371450661927	26.166983699478497	31.205629273862584	14.31601557599699
4	23.9524476383829	22.172882301888627	36.03600605221185	17.838664007516623
5	22.624366041049562	33.198839736713424	27.4776562271986	16.699137995038416
6	22.131023730097976	32.34579532566481	31.092854344970956	14.430326599266257
7	70.73266581498767	4.118909065773002	20.612466429937456	4.535958689301871
8	70.34659409474118	6.517520436455617	17.700671529258756	5.435213939544446
9	64.71834429507349	5.325255183678485	19.213596483367937	10.742804037880086
10	33.553805545812736	25.321747768182878	25.741997588331827	15.38244909767256
11	26.157255101753453	22.135504005366087	33.049070534893666	18.658170357986794
12	21.883584527433367	19.70437863702346	36.891482612691725	21.52055422285145
13	22.232021935427714	20.87961884378176	39.52345232091059	17.364906899879927
14	18.75558434310204	25.818034260024938	36.17873482146743	19.247646575405593
15	17.726017086489794	23.15636672717172	39.26820463849299	19.8494115478455
16	20.74764273516965	24.781938602307726	33.619985611916	20.85043305060663
17	19.846211351225417	25.101958264315762	34.03703523544487	21.014795149013956
18	21.783098353562842	22.705779043064407	33.96867903563995	21.542443567732803
19	19.691065819083924	25.852724391386605	31.45742074393051	22.99878904559896
20	22.42441775622694	25.532320705784162	32.996843326053956	19.04641821193494
21	21.16635646094096	27.43272546665267	33.38573121932612	18.01518685308025
22	20.456296834877534	21.190037915929555	34.99491808776731	23.358747161425597
23	20.055248194449067	25.21230104377613	34.40403378383568	20.328416977939124
24	21.08596752184454	23.822263639878035	33.820957959657036	21.270810878620384
25	20.804350219277474	27.740456373639592	31.027058302462102	20.42813510462083
26	20.129620763899734	23.769268383849504	33.148020614386546	22.953090237864217
27	23.215378352846	23.074569701562464	31.106807202234503	22.60324474335703
28	19.10760597131088	25.885622412641034	32.83235321978182	22.174418396266265
29	21.039244651191368	26.29691168225376	31.539729800998973	21.1241138655559
30	21.676851825776176	26.999546852158595	32.256573843896966	19.067027478168256
31	23.481250688042273	24.38217004052729	30.983791644158615	21.15278762727182
32	26.491099613160234	24.684012585733267	30.399691757061554	18.42519604404495
33	26.23009157682648	23.500067844168345	28.959731285890207	21.31010929311497
34	25.780655963502397	24.913274671595822	30.56046963525439	18.745599729647388
35	26.277582494668472	25.046146835261556	29.660702353552605	19.015568316517367
36	24.51837040867791	26.725738029344527	30.655323463073568	18.100568098903995
37	24.403931377543834	24.619240606142842	31.180539732361158	19.796288283952165
38	24.697453411537605	24.503137472766326	29.72995460841114	21.069454507284927
39	26.923766196195093	22.7653027001979	29.25274128842476	21.058189815182246
40	26.795886339256686	22.844539568511088	30.975599140811212	19.383974951421017
41	22.34466885645454	25.272080716639227	30.357705177406103	22.025545249500126
42	23.83455239489914	24.353752294540975	31.464973207953896	20.346722102605984
43	23.639084385344635	23.576488539455866	30.719967434799194	22.064459640400305
44	23.670574320086228	23.213842258468357	30.02795691767302	23.08762650377239
45	23.04858410500741	21.90572988804432	29.63561281205117	25.41007319489709
46	25.400344597172047	23.475234318396524	28.77283980327751	22.351581281153916
47	21.407011246771003	24.20884739158374	32.850914360178294	21.53322700146697
48	22.093517425710633	23.993154139390324	30.7243197022025	23.189008732696536
49	22.73816503285962	21.93990798794678	33.17963855699294	22.14228842220066
50	21.358880289604993	22.367198240659906	33.4727765673923	22.8011449023428
51	21.33955110201971	21.380257603027133	30.966510582410184	26.31368071254298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	623.0
2	943.0
3	12005.0
4	23067.0
5	14818.0
6	6569.0
7	6432.0
8	6295.0
9	6233.0
10	6171.0
11	6117.0
12	6063.0
13	5903.0
14	5743.0
15	5387.0
16	5031.0
17	4832.5
18	4634.0
19	4393.0
20	4152.0
21	3964.0
22	3776.0
23	3963.0
24	4150.0
25	4435.0
26	5510.5
27	6301.0
28	7087.5
29	7874.0
30	8722.0
31	9570.0
32	11146.5
33	12723.0
34	14517.5
35	16312.0
36	18157.0
37	20002.0
38	21536.5
39	23071.0
40	25032.5
41	26994.0
42	29726.0
43	32458.0
44	38084.0
45	43710.0
46	67882.5
47	92055.0
48	81839.5
49	71624.0
50	71861.5
51	72099.0
52	64142.5
53	56186.0
54	50943.5
55	45701.0
56	41714.0
57	37727.0
58	34272.0
59	30817.0
60	27603.0
61	24389.0
62	21977.0
63	19565.0
64	17466.5
65	15368.0
66	12971.0
67	10574.0
68	9089.5
69	7605.0
70	6627.5
71	5650.0
72	4878.0
73	4106.0
74	3569.5
75	2570.5
76	2108.0
77	1587.5
78	1067.0
79	777.0
80	487.0
81	357.5
82	228.0
83	172.0
84	116.0
85	79.0
86	42.0
87	26.0
88	10.0
89	8.5
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	781202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.32162814832863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0422486902891	29.451823908075813
2	9.37787792184079	7.562626127697703
3	4.273201081011422	5.169072749747355
4	2.5886353004494245	4.175119599854345
5	1.8929536564872447	3.816348671944885
6	1.5333297397739947	3.7095810957564312
7	1.256700549508738	3.547054857576411
8	1.047458398958218	3.378818245090954
9	0.8734543988296743	3.169719312671885
>10	4.033825790747205	23.721106892513156
>50	0.0422371123131143	1.167376236700029
>100	0.030077914456752727	2.166637294426192
>500	0.00255982250695191	0.7196750329478575
>1k	0.0047996672005348314	4.767556797685413
>5k	0.0	0.0
>10k+	6.399556267379775E-4	3.47748317731151
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13792	1.7654844713659208	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	13161	1.6847115086750928	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG	4910	0.6285186161837784	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC	4746	0.6075253263560513	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	4708	0.6026610274935292	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	3185	0.40770504939823504	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	3139	0.4018166876172872	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	2401	0.30734688339251565	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	2055	0.2630561621706038	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	1949	0.2494873285014631	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	1884	0.24116681728925424	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1541	0.197260119661752	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1409	0.18036308150772784	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	1371	0.17549878264520571	No Hit
GAATATGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	1237	0.1583457287615751	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC	1229	0.1573216658431494	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1188	0.15207334338621764	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	899	0.11507907045808893	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC	837	0.1071425828402897	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT	813	0.10407039408501259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5601572960642704E-4	0.0	0.0	0.11866329067257893	0.0
2	2.5601572960642704E-4	0.0	0.0	0.7489740169636022	0.0
3	2.5601572960642704E-4	0.0	0.0	1.2182508493321829	0.0
4	2.5601572960642704E-4	0.0	0.0	2.255498577832622	0.0
5	2.5601572960642704E-4	0.0	0.0	5.020468457582034	0.0
6	2.5601572960642704E-4	0.0	0.0	6.951595106003313	0.0
7	2.5601572960642704E-4	0.0	0.0	8.284669010063979	0.0
8	2.5601572960642704E-4	0.0	0.0	9.957731803041979	0.0
9	2.5601572960642704E-4	0.0	0.0	10.886044838594882	0.0
10	2.5601572960642704E-4	0.0	0.0	12.792465969109141	0.0
11	2.5601572960642704E-4	0.0	0.0	13.808720407781854	0.0
12	2.5601572960642704E-4	0.0	0.0	15.299371993415276	0.0
13	2.5601572960642704E-4	0.0	0.0	15.832396742455856	0.0
14	2.5601572960642704E-4	0.0	0.0	16.170593521265946	0.0
15	2.5601572960642704E-4	0.0	0.0	16.53951218762881	0.0
16	2.5601572960642704E-4	0.0	0.0	16.957329858346498	0.0
17	2.5601572960642704E-4	0.0	0.0	17.42148637612295	0.0
18	2.5601572960642704E-4	0.0	0.0	17.92788548928446	0.0
19	2.5601572960642704E-4	0.0	0.0	18.388841810440834	0.0
20	2.5601572960642704E-4	0.0	0.0	18.734335037544707	0.0
21	2.5601572960642704E-4	0.0	0.0	19.080340296107792	0.0
22	2.5601572960642704E-4	0.0	0.0	19.510190706116983	0.0
23	2.5601572960642704E-4	0.0	0.0	19.901382740955604	0.0
24	2.5601572960642704E-4	0.0	0.0	20.23113100068868	0.0
25	2.5601572960642704E-4	0.0	0.0	20.536301750379543	0.0
26	2.5601572960642704E-4	0.0	0.0	20.83020780796772	0.0
27	2.5601572960642704E-4	0.0	0.0	21.161748177808043	0.0
28	2.5601572960642704E-4	0.0	0.0	21.46474279379725	0.0
29	2.5601572960642704E-4	0.0	0.0	21.78335436929245	0.0
30	2.5601572960642704E-4	0.0	0.0	22.24674283988008	0.0
31	2.5601572960642704E-4	0.0	0.0	22.606444939977113	0.0
32	3.8402359440964053E-4	0.0	0.0	23.006853541081565	0.0
33	3.8402359440964053E-4	0.0	0.0	23.370523884987495	0.0
34	3.8402359440964053E-4	0.0	0.0	23.728689890706885	0.0
35	3.8402359440964053E-4	0.0	0.0	24.10810520198361	0.0
36	3.8402359440964053E-4	0.0	0.0	24.4583347200852	0.0
37	5.120314592128541E-4	0.0	0.0	24.81522064715656	0.0
38	5.120314592128541E-4	0.0	0.0	25.201676390997463	0.0
39	5.120314592128541E-4	0.0	0.0	25.6810658446855	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACG	20	7.031899E-4	45.0	1
CAGACGA	20	7.031899E-4	45.0	12
CCCGTAA	30	2.1646065E-6	44.999996	41
CGTTTTT	6670	0.0	44.190403	1
TAATACG	240	0.0	44.062496	4
CGTTATT	760	0.0	42.63158	1
CGTTCTG	265	0.0	41.603775	1
TACGGCT	760	0.0	41.151314	7
TCGACGG	50	1.0804797E-9	40.5	2
ATCGAAT	50	1.0804797E-9	40.5	43
ACGGGAG	475	0.0	40.263157	5
CCGATGA	750	0.0	40.2	18
CGACGGT	40	3.4563345E-7	39.375	28
CGATGAA	800	0.0	39.09375	19
AATACGG	265	0.0	39.056602	5
AGGTACG	35	6.24572E-6	38.571426	1
CCGTAAG	35	6.24572E-6	38.571426	42
ATCCCCG	35	6.24572E-6	38.571426	38
GTGTACG	35	6.24572E-6	38.571426	1
GCGTATG	35	6.24572E-6	38.571426	1
>>END_MODULE