##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936408.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172595
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01203974622672	33.0	31.0	34.0	30.0	34.0
2	32.119186534951766	33.0	31.0	34.0	30.0	34.0
3	32.09817202120571	33.0	31.0	34.0	30.0	34.0
4	35.7284625858223	37.0	35.0	37.0	33.0	37.0
5	31.758370752339292	37.0	35.0	37.0	0.0	37.0
6	33.70165995538689	37.0	35.0	37.0	17.0	37.0
7	35.463344824589356	37.0	35.0	37.0	32.0	37.0
8	35.74840522610736	37.0	35.0	37.0	35.0	37.0
9	37.70846200643124	39.0	37.0	39.0	35.0	39.0
10	37.379188273124946	39.0	37.0	39.0	34.0	39.0
11	37.27971841594484	39.0	37.0	39.0	34.0	39.0
12	36.946655465106176	39.0	35.0	39.0	33.0	39.0
13	36.6860801297836	39.0	35.0	39.0	33.0	39.0
14	37.772525275934996	40.0	36.0	41.0	33.0	41.0
15	38.036368376835945	40.0	36.0	41.0	33.0	41.0
16	38.052614502158235	40.0	36.0	41.0	34.0	41.0
17	38.07552941858107	40.0	36.0	41.0	34.0	41.0
18	38.044746371563484	40.0	36.0	41.0	34.0	41.0
19	37.876867811929664	40.0	36.0	41.0	34.0	41.0
20	37.94045598076422	40.0	35.0	41.0	34.0	41.0
21	37.85394710159622	40.0	35.0	41.0	34.0	41.0
22	37.93263999536487	40.0	35.0	41.0	34.0	41.0
23	37.94911208320055	40.0	35.0	41.0	34.0	41.0
24	37.93027028592949	40.0	35.0	41.0	34.0	41.0
25	37.6717286132275	39.0	35.0	41.0	34.0	41.0
26	37.70496248442887	39.0	35.0	41.0	34.0	41.0
27	37.67346678640749	39.0	35.0	41.0	34.0	41.0
28	37.63801384744633	39.0	35.0	41.0	34.0	41.0
29	37.628459688867004	39.0	35.0	41.0	34.0	41.0
30	37.49084272429676	39.0	35.0	41.0	33.0	41.0
31	37.41077667371592	39.0	35.0	41.0	33.0	41.0
32	37.30062863930009	39.0	35.0	41.0	33.0	41.0
33	37.22308873374084	39.0	35.0	41.0	33.0	41.0
34	37.111886207595816	39.0	35.0	41.0	33.0	41.0
35	37.106092296995854	39.0	35.0	41.0	33.0	41.0
36	37.00456560155277	39.0	35.0	41.0	33.0	41.0
37	36.882140270575626	39.0	35.0	41.0	32.0	41.0
38	36.762745154842264	39.0	35.0	41.0	32.0	41.0
39	36.712430835192215	39.0	35.0	41.0	31.0	41.0
40	36.62066108519946	39.0	35.0	41.0	31.0	41.0
41	36.53119731162548	39.0	35.0	41.0	31.0	41.0
42	36.544818795445984	39.0	35.0	41.0	31.0	41.0
43	36.406083606129954	38.0	35.0	41.0	31.0	41.0
44	36.33634809814885	38.0	35.0	40.0	31.0	41.0
45	36.31587821199919	38.0	35.0	40.0	31.0	41.0
46	36.202149540832586	38.0	35.0	40.0	31.0	41.0
47	36.096086213389725	38.0	35.0	40.0	31.0	41.0
48	36.095107042498334	38.0	35.0	40.0	31.0	41.0
49	36.07888988672905	38.0	35.0	40.0	31.0	41.0
50	35.93148121324488	37.0	35.0	40.0	31.0	41.0
51	34.82542367971262	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	3.0
11	7.0
12	3.0
13	3.0
14	2.0
15	7.0
16	11.0
17	26.0
18	35.0
19	65.0
20	130.0
21	204.0
22	267.0
23	450.0
24	712.0
25	957.0
26	1279.0
27	1542.0
28	1699.0
29	2016.0
30	2530.0
31	3294.0
32	4644.0
33	7273.0
34	13522.0
35	20239.0
36	12833.0
37	19801.0
38	31527.0
39	47477.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.999536487152	20.88704771285379	22.99023726063907	12.123178539355138
2	32.91868246472957	23.562096236855066	25.778267041339554	17.740954257075813
3	31.65908630029839	23.80196413569339	27.464295025927747	17.074654538080477
4	29.405834467974156	25.29331672412295	25.863437527158954	19.437411280743937
5	21.819287928387265	37.33132477765868	22.90332860163968	17.946058692314377
6	24.935253049045453	33.92102899852255	25.53202584084128	15.611692111590719
7	77.9269387873345	6.251629537356239	10.349662504707553	5.471769170601697
8	78.97679538804717	5.21857527738347	9.430748283553985	6.373881051015383
9	73.4847475303456	7.094643529650338	12.057127958515599	7.363480981488456
10	44.489121932848576	22.60725976998175	18.05440482053362	14.849213476636056
11	36.28436513224601	21.82566123004722	23.583533706074917	18.306439931631854
12	30.596483096265825	21.126915611692112	30.07445175120948	18.202149540832586
13	24.56907789912802	28.478808771980646	30.92094208986355	16.03117123902778
14	18.987803818187086	31.987021640256092	29.26272487615516	19.762449665401665
15	15.717720675569977	23.759089197253687	42.18372490512471	18.339465222051622
16	19.710883861062023	21.921840146006545	37.55265216257713	20.814623830354297
17	19.482024392363627	22.535415278542253	28.563399866740056	29.419160462354064
18	23.473449404675687	22.384194211883308	33.09887308438831	21.043483299052696
19	24.659462904487384	24.465366899388744	27.702424751586086	23.17274544453779
20	25.7997045105594	24.459572988788782	27.77426924302558	21.966453257626235
21	21.728323531967902	27.704162924766067	29.48868738955358	21.07882615371245
22	21.10258118717228	24.57313363654799	28.189692633042675	26.134592543237055
23	18.560792606970075	26.558127408094094	28.41159940902112	26.469480575914712
24	21.997740374866016	23.728381471073902	30.999739274023003	23.274138880037082
25	18.677829601089254	25.470610388481706	31.022914916422838	24.8286450940062
26	17.368985196558416	28.766766128798633	29.11613893797619	24.748109736666763
27	19.099046901706306	29.265042440395145	29.36411831165445	22.271792346244098
28	15.92166632868855	27.108548915090243	34.105275355601265	22.864509400619948
29	18.722442712708943	25.438164489121935	32.27555838813407	23.563834410035053
30	21.389379761870273	28.894811553057735	28.65146730785944	21.06434137721255
31	24.248095251890263	30.040267678669718	22.892899562559748	22.818737506880268
32	23.750398331353747	29.33920449607463	23.219096729337465	23.691300443234162
33	25.7713143486196	28.613227497899707	23.009357165618933	22.60610098786176
34	19.79315739158145	29.823575422231237	25.892986471218748	24.490280714968566
35	19.22998928126539	27.91158492424462	26.882007010631824	25.97641878385816
36	26.666473536313333	28.306729627161854	25.51985862858136	19.506938207943453
37	20.278107708798053	29.984066745850114	27.86697181262493	21.870853732726907
38	20.173237926938786	29.540832584953215	23.36742084069643	26.91850864741157
39	22.84538949564008	26.697760653553114	26.91561169211159	23.54123815869521
40	24.644978127987486	25.219154668443466	26.580723659433936	23.555143544135117
41	19.714939598481994	26.350705408615543	25.2712998638431	28.663055129059355
42	23.397549175816216	25.375590254642372	25.24001274660332	25.986847822938092
43	23.598018482574815	25.462498913641763	25.99264173353805	24.94684087024537
44	20.832584953214173	27.176337669109767	26.836814507952145	25.15426286972392
45	20.130362988499087	28.04426547698369	24.963063819925257	26.862307714591964
46	24.50592427358846	27.715171354905994	25.439323271241925	22.339581100263622
47	19.69292273820215	27.147947507169963	28.142182566123005	25.01694718850488
48	21.639097308728527	23.36162693009647	28.342072481821607	26.657203279353396
49	22.613633071641704	20.746255685274775	31.739042266577826	24.90106897650569
50	20.053883368579623	24.2573655088502	29.92960398621049	25.759147136359683
51	19.413656247284106	25.17570033894377	25.9717836553782	29.438859758393924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	182.5
2	276.0
3	608.5
4	941.0
5	645.5
6	350.0
7	339.5
8	329.0
9	356.0
10	383.0
11	382.0
12	381.0
13	374.5
14	368.0
15	403.0
16	438.0
17	454.5
18	471.0
19	469.0
20	467.0
21	402.0
22	337.0
23	387.0
24	437.0
25	553.5
26	826.5
27	983.0
28	1276.5
29	1570.0
30	1856.0
31	2142.0
32	2337.5
33	2533.0
34	2741.5
35	2950.0
36	3602.0
37	4254.0
38	4850.5
39	5447.0
40	6534.5
41	7622.0
42	9496.0
43	11370.0
44	13252.0
45	15134.0
46	16693.5
47	18253.0
48	19543.5
49	20834.0
50	19684.0
51	18534.0
52	16159.0
53	13784.0
54	11568.5
55	9353.0
56	8443.5
57	7534.0
58	6760.5
59	5987.0
60	5515.0
61	5043.0
62	4710.0
63	4377.0
64	3828.5
65	3280.0
66	2733.5
67	2187.0
68	1743.5
69	1300.0
70	1091.0
71	882.0
72	711.0
73	540.0
74	422.5
75	261.5
76	218.0
77	171.5
78	125.0
79	87.5
80	50.0
81	45.5
82	41.0
83	29.0
84	17.0
85	9.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	172595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.65114864277644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55195124953835	27.69315449462615
2	10.91653330050474	8.220400359222458
3	4.10254831958636	4.6339696978475615
4	2.0928228487012186	3.1518873663779368
5	1.2987812384586974	2.445030273182885
6	0.8448233411301245	1.9085141516266404
7	0.6324633756001478	1.6669080796083318
8	0.4370306536993721	1.3163764883107854
9	0.41087036809060695	1.392276717170254
>10	5.630616767204235	43.139720154118024
>50	0.05385941154745783	1.3517193429705379
>100	0.021543764618983136	1.57710246530896
>500	0.006155361319709468	1.5029404096294794
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	890	0.5156580433963904	No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	666	0.3858744459572989	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	525	0.30418030649787076	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	513	0.2972276137779194	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	315	0.18250818389872245	No Hit
TCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	287	0.16628523421883598	No Hit
AGGAATGGGCACATTCAGCTATGCAGTCTTTTCTATTGCTGAAATATGCAT	283	0.16396766997885223	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	276	0.15991193255888062	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	251	0.14542715605898202	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	248	0.14368898287899418	No Hit
ACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	203	0.11761638517917669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31982386511776123	0.0
2	0.0	0.0	0.0	1.57999942060894	0.0
3	0.0	0.0	0.0	2.0579970451055942	0.0
4	0.0	0.0	0.0	2.6843187809612097	0.0
5	0.0	0.0	0.0	4.471740201048698	0.0
6	0.0	0.0	0.0	5.489730293461572	0.0
7	0.0	0.0	0.0	6.592311480633854	0.0
8	0.0	0.0	0.0	8.362351168921464	0.0
9	0.0	0.0	0.0	9.112083200556215	0.0
10	0.0	0.0	0.0	10.681074191025232	0.0
11	0.0	0.0	0.0	12.700831426171094	0.0
12	0.0	0.0	0.0	14.37005707001941	0.0
13	0.0	0.0	0.0	15.011442973434919	0.0
14	0.0	0.0	0.0	15.245516961673282	0.0
15	0.0	0.0	0.0	15.748428401749761	0.0
16	0.0	0.0	0.0	16.73165503056288	0.0
17	0.0	0.0	0.0	17.932732697934473	0.0
18	0.0	0.0	0.0	19.374837046264375	0.0
19	0.0	0.0	0.0	20.114719429879198	0.0
20	0.0	0.0	0.0	20.801877227034385	0.0
21	0.0	0.0	0.0	21.658217213708394	0.0
22	0.0	0.0	0.0	22.472261653002693	0.0
23	0.0	0.0	0.0	23.300211477736898	0.0
24	0.0	0.0	0.0	23.87728497349286	0.0
25	0.0	0.0	0.0	24.436397346388944	0.0
26	0.0	0.0	0.0	24.972334076885193	0.0
27	0.0	0.0	0.0	25.556360265361107	0.0
28	0.0	0.0	0.0	26.08302673889742	0.0
29	0.0	0.0	0.0	26.56681827399403	0.0
30	0.0	0.0	0.0	27.18850488136968	0.0
31	0.0	0.0	0.0	27.738346997305833	0.0
32	0.0	0.0	0.0	28.23604391784235	0.0
33	0.0	0.0	0.0	28.780671514238534	0.0
34	0.0	0.0	0.0	29.30559981459486	0.0
35	0.0	0.0	0.0	29.90121382427069	0.0
36	0.0	0.0	0.0	30.41107795706712	0.0
37	0.0	0.0	0.0	30.913989397143602	0.0
38	0.0	0.0	0.0	31.419218401460064	0.0
39	0.0	0.0	0.0	31.94414670181639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.1547512E-6	45.000004	2
CGCGGGT	30	2.1547512E-6	45.000004	4
CACATCC	25	3.87699E-5	45.000004	45
TTGTCGG	30	2.1547512E-6	45.000004	2
CAATTGC	25	3.87699E-5	45.000004	28
GCACATT	30	2.1547512E-6	45.000004	9
GTTGTGG	30	2.1547512E-6	45.000004	2
ATTGGTA	25	3.87699E-5	45.000004	15
TCATCCA	30	2.1547512E-6	45.000004	27
GGCGATA	50	2.1827873E-11	45.000004	8
ACATAAC	25	3.87699E-5	45.000004	31
TCGACGT	25	3.87699E-5	45.000004	26
TAGTCGG	25	3.87699E-5	45.000004	2
TGCGTAG	25	3.87699E-5	45.000004	1
GCCCCCG	20	7.0166134E-4	45.0	9
ACACGCG	40	6.7575456E-9	45.0	36
CGAGTAC	20	7.0166134E-4	45.0	35
GGGTATA	40	6.7575456E-9	45.0	7
GACGTAG	20	7.0166134E-4	45.0	1
TCTTAGG	20	7.0166134E-4	45.0	2
>>END_MODULE