##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267324
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897076207149375	33.0	31.0	34.0	30.0	34.0
2	32.008525235295	33.0	31.0	34.0	30.0	34.0
3	31.975744789094882	33.0	31.0	34.0	30.0	34.0
4	35.61469976507908	37.0	35.0	37.0	33.0	37.0
5	32.206670557076805	37.0	35.0	37.0	16.0	37.0
6	33.86737816282863	37.0	35.0	37.0	19.0	37.0
7	35.399777049572805	37.0	35.0	37.0	32.0	37.0
8	35.5747220601218	37.0	35.0	37.0	33.0	37.0
9	37.48211159491853	39.0	37.0	39.0	35.0	39.0
10	37.11936825724589	39.0	37.0	39.0	34.0	39.0
11	37.0775762744834	39.0	37.0	39.0	33.0	39.0
12	36.889796651254656	39.0	35.0	39.0	33.0	39.0
13	36.73687360655983	39.0	35.0	39.0	33.0	39.0
14	37.89542652361928	40.0	36.0	41.0	33.0	41.0
15	38.011181188370664	40.0	36.0	41.0	33.0	41.0
16	38.074276907423204	40.0	36.0	41.0	34.0	41.0
17	37.97784710688153	40.0	36.0	41.0	33.0	41.0
18	37.97422603282908	40.0	36.0	41.0	33.0	41.0
19	37.865156140114614	40.0	36.0	41.0	33.0	41.0
20	37.78551869641334	40.0	36.0	41.0	33.0	41.0
21	37.84185482784935	40.0	36.0	41.0	33.0	41.0
22	37.88639628316201	40.0	36.0	41.0	33.0	41.0
23	37.86311367479164	40.0	36.0	41.0	34.0	41.0
24	37.86824602355195	40.0	36.0	41.0	33.0	41.0
25	37.69561281441247	40.0	35.0	41.0	33.0	41.0
26	37.69275485927189	40.0	35.0	41.0	33.0	41.0
27	37.62517020544358	40.0	35.0	41.0	33.0	41.0
28	37.51757792042615	40.0	35.0	41.0	33.0	41.0
29	37.463426403914355	40.0	35.0	41.0	33.0	41.0
30	37.35180155915668	40.0	35.0	41.0	33.0	41.0
31	37.4123610300609	40.0	35.0	41.0	33.0	41.0
32	37.21865227214915	40.0	35.0	41.0	32.0	41.0
33	37.1474428034894	40.0	35.0	41.0	32.0	41.0
34	37.08357274318804	40.0	35.0	41.0	32.0	41.0
35	37.01336580329488	40.0	35.0	41.0	32.0	41.0
36	36.977697475722344	40.0	35.0	41.0	32.0	41.0
37	36.852067154464244	39.0	35.0	41.0	31.0	41.0
38	36.80270757582559	39.0	35.0	41.0	31.0	41.0
39	36.68143526207898	39.0	35.0	41.0	31.0	41.0
40	36.60915967140998	39.0	35.0	41.0	31.0	41.0
41	36.57953270188984	39.0	35.0	41.0	30.0	41.0
42	36.52286364112463	39.0	35.0	41.0	31.0	41.0
43	36.459741736619236	39.0	35.0	41.0	31.0	41.0
44	36.45004563750356	39.0	35.0	41.0	31.0	41.0
45	36.45919932366716	39.0	35.0	41.0	31.0	41.0
46	36.34328754619862	39.0	35.0	41.0	30.0	41.0
47	36.13945998114647	39.0	35.0	41.0	30.0	41.0
48	36.159215034938875	38.0	35.0	40.0	30.0	41.0
49	36.16351693076566	39.0	35.0	41.0	30.0	41.0
50	36.04991321392767	38.0	35.0	40.0	30.0	41.0
51	35.02199203962233	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	11.0
11	6.0
12	2.0
13	9.0
14	6.0
15	8.0
16	13.0
17	28.0
18	59.0
19	134.0
20	338.0
21	613.0
22	1021.0
23	1367.0
24	1744.0
25	1954.0
26	2156.0
27	2424.0
28	2571.0
29	3160.0
30	4074.0
31	5383.0
32	7372.0
33	10746.0
34	18175.0
35	24866.0
36	19362.0
37	29643.0
38	48767.0
39	81248.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.73429995062172	19.39332046505364	22.038425281680656	11.833954302643983
2	28.922206760335772	30.102048450569345	23.556433391689485	17.419311397405394
3	29.46461971240891	30.6119166255181	25.03478924451228	14.888674417560713
4	26.24268677709446	24.175532312848826	31.744998578503985	17.836782331552723
5	26.151785847884963	34.354565994822764	21.34525893672098	18.148389220571293
6	22.488815070850354	39.434169771513226	23.29831964208227	14.778695515554158
7	75.42757103739282	5.148434109919049	15.005386721730934	4.418608130957191
8	75.25961006119914	9.863686013975551	9.162289955260283	5.7144139695650225
9	69.97912645329262	6.116547709895109	10.556852358935224	13.347473477877033
10	42.291750834193714	24.161317352725533	18.735317442504225	14.811614370576528
11	33.23457676826622	22.925363977794735	24.863835645134742	18.976223608804297
12	27.44572129700289	19.60280408792327	30.44058894824258	22.51088566683126
13	24.53614340650297	24.881791384237854	33.01723750953899	17.56482769972019
14	19.69894210770451	31.391494964911494	28.398497703161706	20.511065224222293
15	17.205712917657973	24.015426972512756	39.06869566518532	19.71016444464395
16	19.000538672173093	24.350226691206174	30.915667878679052	25.733566757941674
17	18.973605063518427	25.08341937124987	29.820741871287275	26.122233693944423
18	21.160090377220154	23.229489308853676	31.432643533689454	24.17777678023672
19	21.51957923718035	26.19630111774476	27.61443042899253	24.669689216082357
20	23.881507085035388	25.653888165671617	30.284224386886326	20.180380362406666
21	21.40585955619398	30.032844039442775	28.46396133530846	20.097335069054783
22	19.320375274947253	24.763956846373688	29.645673415031947	26.269994463647112
23	20.309063159312295	28.24998877766306	27.985141625892172	23.455806437132466
24	21.322814262842094	24.848498451317504	29.052011790935346	24.77667549490506
25	18.278194251170866	30.314150618724845	27.050320958836465	24.357334171267826
26	19.160269934611183	25.518097888704343	29.74592629169098	25.575705884993493
27	22.141296703625564	26.45067408837216	26.56401969146055	24.844009516541725
28	17.433900435426672	25.924346485912224	32.01433466505065	24.62741841361045
29	22.306639134533377	23.76329846927324	29.202017028025917	24.728045368167468
30	20.050949409705076	27.081743502266914	29.257754634825154	23.609552453202856
31	23.328245873920785	24.935284523649205	23.11128069309153	28.62518890933848
32	25.45824542502731	29.642680791848093	22.92686028938666	21.972213493737936
33	21.397255764540407	25.896290643563614	26.133456030883874	26.57299756101211
34	22.26960542263321	24.11231314808996	26.20191228621448	27.41616914306235
35	19.383968517604107	28.39774954736574	24.938651224731036	27.279630710299113
36	23.58336700034415	27.17713336625219	25.285795514057845	23.95370411934581
37	18.664990797683707	28.382038275650523	27.528018434558817	25.424952492106957
38	18.402014035402733	28.31320794242193	25.33966273136718	27.945115290808157
39	20.702593107988807	28.034893986323713	27.214915233948318	24.04759767173916
40	20.984273765168858	24.900495279136926	29.29142164564349	24.823809310050727
41	18.005117385644386	27.871796022803792	24.76208645688378	29.361000134668043
42	20.460938785892775	26.994957429935212	27.39783932606126	25.146264458110757
43	22.15326719636097	26.455163023147943	25.24651733476979	26.145052445721294
44	20.539869222366868	24.704104382696652	28.33078960362706	26.42523679130942
45	19.46663973305801	24.224536517484403	26.651553919588217	29.65726982986937
46	24.299726174978677	26.63733895946492	26.30628001975131	22.75665484580509
47	18.47982223818288	24.896006344361147	32.38766440723616	24.236507010219807
48	20.420164295012793	25.289536293037663	27.38811330071374	26.902186111235803
49	19.733731352216786	22.298783498675764	32.965988837515525	25.001496311591925
50	21.25061722853167	23.20704463497479	29.931468929089792	25.610869207403752
51	19.84333617632536	22.99718693420718	26.66315033442564	30.49632655504182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	179.0
2	283.0
3	639.0
4	995.0
5	696.0
6	397.0
7	382.5
8	368.0
9	407.5
10	447.0
11	463.5
12	480.0
13	456.5
14	433.0
15	458.0
16	483.0
17	549.0
18	615.0
19	574.5
20	534.0
21	559.0
22	584.0
23	699.5
24	815.0
25	891.5
26	1362.5
27	1757.0
28	2200.0
29	2643.0
30	2812.5
31	2982.0
32	3728.5
33	4475.0
34	5015.5
35	5556.0
36	6194.5
37	6833.0
38	7869.5
39	8906.0
40	10071.5
41	11237.0
42	13247.0
43	15257.0
44	17898.5
45	20540.0
46	29809.5
47	39079.0
48	33822.0
49	28565.0
50	28485.5
51	28406.0
52	24292.5
53	20179.0
54	18098.5
55	16018.0
56	13975.0
57	11932.0
58	11027.5
59	10123.0
60	9034.0
61	7945.0
62	7046.5
63	6148.0
64	5073.5
65	3999.0
66	3171.0
67	2343.0
68	2215.5
69	2088.0
70	1542.5
71	997.0
72	816.5
73	636.0
74	590.5
75	400.5
76	256.0
77	218.0
78	180.0
79	148.5
80	117.0
81	89.5
82	62.0
83	44.0
84	26.0
85	21.0
86	16.0
87	8.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	267324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.33970762071494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51461660221604	27.823539973964177
2	10.075337113546654	7.524202839999401
3	3.5404436073654053	3.965973874399605
4	1.9224989480855155	2.871421944905807
5	1.2212226251778238	2.280004788197094
6	0.9226792762828349	2.0671544642456343
7	0.6802380332204612	1.7779922491059537
8	0.6071049309743733	1.8135296494141941
9	0.5780520547396261	1.942586524217803
>10	5.853653649642349	38.10656731157696
>50	0.050091165921978	1.2108901557660368
>100	0.022040113005670322	1.7005581242237884
>500	0.00801458654751648	1.9553051727491733
>1k	0.00400729327375824	4.960272927234367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	3895	1.4570334126378477	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	3785	1.4158848438598852	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	3768	1.4095255195942002	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1812	0.6778291511424339	No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	929	0.3475183672247909	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT	829	0.31011057742664333	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT	623	0.23305053044245932	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT	613	0.22930975146264457	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCATCAAT	611	0.22856159566668166	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTA	556	0.20798731127770045	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC	534	0.19975759752210803	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.19900944172614507	No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT	475	0.17768700154120093	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	407	0.1522497044784606	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	400	0.14963115919259026	No Hit
TCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	328	0.12269755053792401	No Hit
GAATATGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	323	0.12082716104801663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7407789798147564E-4	0.0	0.0	0.1859167152967934	0.0
2	3.7407789798147564E-4	0.0	0.0	1.491822657150125	0.0
3	3.7407789798147564E-4	0.0	0.0	1.9059268902156186	0.0
4	3.7407789798147564E-4	0.0	0.0	2.6170489742784038	0.0
5	3.7407789798147564E-4	0.0	0.0	5.344076850563361	0.0
6	3.7407789798147564E-4	0.0	0.0	6.382891173257919	0.0
7	3.7407789798147564E-4	0.0	0.0	7.511484191468031	0.0
8	3.7407789798147564E-4	0.0	0.0	9.107674582154988	0.0
9	3.7407789798147564E-4	0.0	0.0	9.627268782451258	0.0
10	3.7407789798147564E-4	0.0	0.0	12.273869910670198	0.0
11	3.7407789798147564E-4	0.0	0.0	13.782526073229489	0.0
12	3.7407789798147564E-4	0.0	0.0	16.572773114273318	0.0
13	3.7407789798147564E-4	0.0	0.0	17.209079618739807	0.0
14	3.7407789798147564E-4	0.0	0.0	17.53003845520791	0.0
15	3.7407789798147564E-4	0.0	0.0	18.129685325672217	0.0
16	3.7407789798147564E-4	0.0	0.0	18.98482740045787	0.0
17	3.7407789798147564E-4	0.0	0.0	19.924511080187337	0.0
18	3.7407789798147564E-4	0.0	0.0	21.002977660067934	0.0
19	3.7407789798147564E-4	0.0	0.0	21.860738280139458	0.0
20	3.7407789798147564E-4	0.0	0.0	22.484326136074575	0.0
21	3.7407789798147564E-4	0.0	0.0	23.13933653544014	0.0
22	3.7407789798147564E-4	0.0	0.0	23.850458619502927	0.0
23	3.7407789798147564E-4	0.0	0.0	24.54886205503434	0.0
24	3.7407789798147564E-4	0.0	0.0	25.107734434618663	0.0
25	3.7407789798147564E-4	0.0	0.0	25.601891337852194	0.0
26	3.7407789798147564E-4	0.0	0.0	26.10240756535141	0.0
27	3.7407789798147564E-4	0.0	0.0	26.60442010444255	0.0
28	3.7407789798147564E-4	0.0	0.0	27.04583202406069	0.0
29	7.481557959629513E-4	0.0	0.0	27.498840358516258	0.0
30	7.481557959629513E-4	0.0	0.0	28.040879232691417	0.0
31	7.481557959629513E-4	0.0	0.0	28.537654681210814	0.0
32	7.481557959629513E-4	0.0	0.0	29.00787059897353	0.0
33	7.481557959629513E-4	0.0	0.0	29.501279346411096	0.0
34	7.481557959629513E-4	0.0	0.0	29.98645838009307	0.0
35	7.481557959629513E-4	0.0	0.0	30.53148987745208	0.0
36	0.001122233693944427	0.0	0.0	30.975894420254075	0.0
37	0.001122233693944427	0.0	0.0	31.4236656641379	0.0
38	0.001122233693944427	0.0	0.0	31.910715087309782	0.0
39	0.001122233693944427	0.0	0.0	32.37831245978663	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	20	7.023563E-4	45.000004	1
CACGGGC	40	6.7811925E-9	45.000004	4
CCAATCG	20	7.023563E-4	45.000004	40
CACGAAT	20	7.023563E-4	45.000004	20
GCGCAAT	20	7.023563E-4	45.000004	19
TCAGGCG	20	7.023563E-4	45.000004	1
TCGGCTT	20	7.023563E-4	45.000004	22
TTGAGTG	20	7.023563E-4	45.000004	32
GTAATAC	20	7.023563E-4	45.000004	40
TGTTGCG	20	7.023563E-4	45.000004	1
TCGTGCG	20	7.023563E-4	45.000004	1
GTGCTAG	20	7.023563E-4	45.000004	1
CTCGGAT	20	7.023563E-4	45.000004	39
GCCCTAG	20	7.023563E-4	45.000004	20
CGTAAGC	20	7.023563E-4	45.000004	43
CGGTAGT	20	7.023563E-4	45.000004	12
CAATCGG	20	7.023563E-4	45.000004	22
CTAGCGG	20	7.023563E-4	45.000004	2
GTCCTCC	25	3.8827482E-5	45.0	26
TCGATCA	25	3.8827482E-5	45.0	17
>>END_MODULE