##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936406.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532711
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932045705832994	33.0	31.0	34.0	30.0	34.0
2	32.03148048378952	33.0	31.0	34.0	30.0	34.0
3	31.996260636630367	33.0	31.0	34.0	30.0	34.0
4	35.65518451843495	37.0	35.0	37.0	33.0	37.0
5	32.03114258950913	37.0	35.0	37.0	0.0	37.0
6	33.811577008922285	37.0	35.0	37.0	17.0	37.0
7	35.49019637289262	37.0	35.0	37.0	32.0	37.0
8	35.733048500969566	37.0	35.0	37.0	35.0	37.0
9	37.65567821952241	39.0	37.0	39.0	35.0	39.0
10	37.229274409576675	39.0	37.0	39.0	34.0	39.0
11	37.216252339448594	39.0	37.0	39.0	34.0	39.0
12	37.265066799822044	39.0	37.0	39.0	34.0	39.0
13	37.23643589113046	39.0	37.0	39.0	34.0	39.0
14	38.42869961386192	40.0	38.0	41.0	34.0	41.0
15	38.55625845908945	40.0	38.0	41.0	34.0	41.0
16	38.50003848240416	40.0	38.0	41.0	34.0	41.0
17	38.49986765807351	40.0	38.0	41.0	34.0	41.0
18	38.424806320875675	40.0	38.0	41.0	34.0	41.0
19	38.29637458209048	40.0	37.0	41.0	34.0	41.0
20	38.37909485631046	40.0	37.0	41.0	34.0	41.0
21	38.333711900073396	40.0	37.0	41.0	34.0	41.0
22	38.43012815579179	40.0	38.0	41.0	34.0	41.0
23	38.417350120421766	40.0	38.0	41.0	34.0	41.0
24	38.38007662691403	40.0	37.0	41.0	34.0	41.0
25	38.132531522720576	40.0	37.0	41.0	34.0	41.0
26	38.20017983484478	40.0	37.0	41.0	34.0	41.0
27	38.19124253112851	40.0	37.0	41.0	34.0	41.0
28	38.14044951202435	40.0	37.0	41.0	34.0	41.0
29	38.087159829626195	40.0	37.0	41.0	34.0	41.0
30	37.957012338772806	40.0	37.0	41.0	34.0	41.0
31	37.93571749034655	40.0	37.0	41.0	33.0	41.0
32	37.78856828561828	40.0	37.0	41.0	33.0	41.0
33	37.738464195408014	40.0	37.0	41.0	33.0	41.0
34	37.70629290553415	40.0	37.0	41.0	33.0	41.0
35	37.66071284430019	40.0	37.0	41.0	33.0	41.0
36	37.57613790591897	40.0	37.0	41.0	33.0	41.0
37	37.47785384570621	40.0	36.0	41.0	33.0	41.0
38	37.31941521763208	40.0	36.0	41.0	32.0	41.0
39	37.23671183812611	40.0	36.0	41.0	32.0	41.0
40	37.25183448436394	40.0	36.0	41.0	32.0	41.0
41	37.196498664379	40.0	36.0	41.0	32.0	41.0
42	37.19662068175803	40.0	36.0	41.0	32.0	41.0
43	37.09754820155769	39.0	36.0	41.0	32.0	41.0
44	37.036045810955656	39.0	35.0	41.0	32.0	41.0
45	37.013864928638604	39.0	35.0	41.0	32.0	41.0
46	36.908802333723166	39.0	35.0	41.0	31.0	41.0
47	36.816268107848344	39.0	35.0	41.0	31.0	41.0
48	36.78520999190931	39.0	35.0	41.0	31.0	41.0
49	36.73936712401283	39.0	35.0	41.0	31.0	41.0
50	36.62178179162811	39.0	35.0	41.0	31.0	41.0
51	35.586522523469576	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	18.0
11	14.0
12	10.0
13	5.0
14	11.0
15	10.0
16	32.0
17	44.0
18	125.0
19	169.0
20	344.0
21	656.0
22	953.0
23	1437.0
24	1975.0
25	2811.0
26	3500.0
27	4188.0
28	4629.0
29	5551.0
30	6874.0
31	9227.0
32	12257.0
33	17614.0
34	28081.0
35	37813.0
36	41967.0
37	65847.0
38	112539.0
39	173878.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82488816637915	20.011976475049323	24.130344595850282	12.03279076272125
2	32.06335142319194	25.308093882048617	24.796934923438787	17.831619771320657
3	31.886332364077337	24.87577692219609	26.926232047019866	16.311658666706712
4	27.598829384037497	25.853605425831265	27.6493258070511	18.89823938308013
5	23.716424102374457	36.627927713150285	22.13282624162069	17.52282194285457
6	23.951448346289077	35.58252035343742	24.34716009243286	16.118871207840648
7	80.485103555211	4.685842792808859	10.17925291574606	4.649800736234093
8	80.51119650241876	5.61035908776053	8.467067509400032	5.411376900420679
9	74.59673256230865	7.071564131395823	10.648925965485976	7.682777340809557
10	32.792639911696966	35.65610621894423	17.354437959794335	14.196815909564473
11	25.590798763306932	25.93225970554391	29.575698643354464	18.901242887794695
12	23.7502135304133	22.76318679358977	32.305133552714324	21.181466123282604
13	22.899283100968443	25.23300626418452	33.9713277931186	17.896382841728443
14	20.043700993596904	28.895967982639743	30.001633155688545	21.05869786807481
15	18.48488204673829	26.697027093489716	34.53974106034979	20.2783497994222
16	21.053066296735	27.241412323004404	30.396218587564366	21.30930279269623
17	21.27288529803214	25.567709320813726	29.64440381370011	23.51500156745402
18	22.10053856593913	24.523428275368822	31.759809727976336	21.616223430715717
19	20.66392471715433	27.45353484347047	29.0033432761854	22.879197163189797
20	22.610946648370316	27.05594590688009	29.856526334166176	20.47658111058341
21	21.621104125876883	28.348954686499813	29.87285789105162	20.157083296571688
22	21.34290450169041	24.825468218227144	28.770008503672724	25.061618776409723
23	19.13514081744135	28.162737394196853	29.613805609420492	23.0883161789413
24	20.402432087942618	26.101206845738123	30.003322627090483	23.493038439228776
25	19.33937913803169	29.070171256084443	28.45801945144741	23.13243015443646
26	20.028871189068745	27.058949411594657	29.020988866383462	23.89119053295314
27	22.36052944279356	25.75167398458076	28.128947966158012	23.758848606467673
28	19.012935719367537	26.468197578048887	30.430195734647867	24.08867096793571
29	20.539654709589254	23.85364672402109	29.69095813677585	25.915740429613805
30	23.50411386286373	24.524930027726104	29.387979598694226	22.58297651071594
31	23.706287273962808	25.60356365834383	27.178151004953904	23.51199806273946
32	23.600789170863752	25.00924516294952	28.52428427421247	22.86568139197426
33	22.36184347610618	24.00269564548132	29.92673325686911	23.70872762154339
34	21.173957361496196	23.25238262397435	31.689790524318063	23.88386949021139
35	22.25521905873917	22.974182999787878	30.479753562438173	24.290844379034787
36	23.842946738475458	23.92272733245606	30.771281238795517	21.463044690272962
37	22.690914961395578	23.932301003733734	32.522887644520196	20.853896390350492
38	22.23175417815664	25.473849798483606	30.948863455044105	21.345532568315654
39	21.349474668253517	23.034440813123812	33.547082752186455	22.069001766436212
40	22.899658539057764	22.514459059414953	32.91127834792223	21.67460405360505
41	21.43019385745742	23.76354158258418	29.947757789871055	24.858506770087345
42	24.249733908254196	22.82607267355095	29.80997201109044	23.114221407104417
43	23.548227838358883	23.52908049580354	29.925231504511828	22.997460161325748
44	22.168305141061477	23.716236383329797	29.60535825241078	24.510100223197945
45	21.880719564641993	22.147092889014868	29.88599822417784	26.086189322165303
46	24.431070505395983	23.19099849637045	29.72962825997586	22.648302738257705
47	21.335395739904	22.71531843720141	33.11345175902131	22.835834063873282
48	22.816123564183957	22.165864793480893	30.932907336247982	24.085104306087164
49	23.212210748417057	20.780685962933	33.30229711794951	22.704806170700436
50	21.933280897146858	21.064141720369957	32.796206573545504	24.20637080893768
51	21.353416768191384	20.36413740283193	29.8403824963254	28.442063332651284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	258.5
2	396.0
3	1501.5
4	2607.0
5	1729.5
6	852.0
7	881.5
8	911.0
9	940.5
10	970.0
11	1028.0
12	1086.0
13	1052.0
14	1018.0
15	1032.5
16	1047.0
17	992.0
18	937.0
19	974.0
20	1011.0
21	1082.0
22	1153.0
23	1446.5
24	1740.0
25	2100.0
26	2938.0
27	3416.0
28	4117.0
29	4818.0
30	5859.0
31	6900.0
32	8358.5
33	9817.0
34	11331.0
35	12845.0
36	14741.0
37	16637.0
38	17954.5
39	19272.0
40	22167.5
41	25063.0
42	28664.5
43	32266.0
44	36644.5
45	41023.0
46	51705.5
47	62388.0
48	64044.5
49	65701.0
50	64733.0
51	63765.0
52	54950.0
53	46135.0
54	38863.0
55	31591.0
56	27182.0
57	22773.0
58	19344.0
59	15915.0
60	13939.5
61	11964.0
62	10170.5
63	8377.0
64	6969.5
65	5562.0
66	4545.5
67	3529.0
68	2839.0
69	2149.0
70	1784.0
71	1419.0
72	1292.0
73	1165.0
74	984.5
75	681.0
76	558.0
77	439.5
78	321.0
79	231.5
80	142.0
81	104.5
82	67.0
83	37.0
84	7.0
85	6.5
86	6.0
87	3.0
88	0.0
89	3.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	532711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.26688062697749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35712704195531	29.13595797109459
2	11.117816064548588	8.953595446077411
3	4.20323718429856	5.077537498410693
4	2.270982515983002	3.6578152750816217
5	1.440874933853495	2.9009769479941374
6	1.0884629898304108	2.62974055670305
7	0.8896778978826595	2.507718759735092
8	0.7415709770926523	2.388860000881671
9	0.6545951215488336	2.372265325656783
>10	5.138801226719066	33.6345726331289
>50	0.06206181606822929	1.675111228754143
>100	0.030560742760130273	2.405700540616476
>500	9.403305464655468E-4	0.23400061920216925
>1k	0.003291156912629414	2.4261471966632677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2457	0.46122569273020453	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	2207	0.4142959315651451	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	2072	0.388953860536013	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	1865	0.3500960182913437	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	1654	0.31048729986803353	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1404	0.26355753870297405	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	1156	0.217003215627235	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	641	0.12032790762721252	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	595	0.11169283157284156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.631571339807138E-4	0.0	0.0	0.22601372977092646	0.0
2	7.508761786409517E-4	0.0	0.0	1.248331646990582	0.0
3	7.508761786409517E-4	0.0	0.0	1.6902222781207823	0.0
4	7.508761786409517E-4	0.0	0.0	2.325088087161707	0.0
5	7.508761786409517E-4	0.0	0.0	4.252399518688369	0.0
6	7.508761786409517E-4	0.0	0.0	5.447794395084765	0.0
7	7.508761786409517E-4	0.0	0.0	6.632113847846205	0.0
8	7.508761786409517E-4	0.0	0.0	8.547035822425293	0.0
9	7.508761786409517E-4	0.0	0.0	9.339773348025478	0.0
10	7.508761786409517E-4	0.0	0.0	11.233858508647279	0.0
11	7.508761786409517E-4	0.0	0.0	13.082140222371981	0.0
12	7.508761786409517E-4	0.0	0.0	14.971907844966596	0.0
13	7.508761786409517E-4	0.0	0.0	15.643378867716267	0.0
14	7.508761786409517E-4	0.0	0.0	15.915571482473611	0.0
15	7.508761786409517E-4	0.0	0.0	16.411149760376638	0.0
16	7.508761786409517E-4	0.0	0.0	17.362509878714725	0.0
17	7.508761786409517E-4	0.0	0.0	18.553399498039276	0.0
18	7.508761786409517E-4	0.0	0.0	19.825196025612385	0.0
19	7.508761786409517E-4	0.0	0.0	20.647780879313547	0.0
20	7.508761786409517E-4	0.0	0.0	21.394902677061296	0.0
21	7.508761786409517E-4	0.0	0.0	22.25090152071198	0.0
22	7.508761786409517E-4	0.0	0.0	23.184803767896664	0.0
23	7.508761786409517E-4	0.0	0.0	24.132221786296885	0.0
24	7.508761786409517E-4	0.0	0.0	24.84536643696113	0.0
25	9.385952233011896E-4	0.0	0.0	25.505386597986526	0.0
26	9.385952233011896E-4	0.0	0.0	26.14025240702745	0.0
27	9.385952233011896E-4	0.0	0.0	26.758598940138274	0.0
28	9.385952233011896E-4	0.0	0.0	27.424438391548136	0.0
29	9.385952233011896E-4	0.0	0.0	28.068502433777414	0.0
30	9.385952233011896E-4	0.0	0.0	28.859550487975657	0.0
31	0.0011263142679614276	0.0	0.0	29.54754078665543	0.0
32	0.0011263142679614276	0.0	0.0	30.159692591292465	0.0
33	0.0011263142679614276	0.0	0.0	30.79455840033339	0.0
34	0.0011263142679614276	0.0	0.0	31.391880400442265	0.0
35	0.0011263142679614276	0.0	0.0	32.06391458032592	0.0
36	0.0011263142679614276	0.0	0.0	32.660861142345475	0.0
37	0.0011263142679614276	0.0	0.0	33.24466737123881	0.0
38	0.0011263142679614276	0.0	0.0	33.83354201433798	0.0
39	0.0011263142679614276	0.0	0.0	34.45752011878861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.029873E-4	45.000004	1
TCTAGCG	20	7.029873E-4	45.000004	1
TGCGCCC	20	7.029873E-4	45.000004	17
TCGCTAG	20	7.029873E-4	45.000004	1
ACGGAGT	20	7.029873E-4	45.000004	5
CTCGCGC	35	1.2102828E-7	45.000004	33
TCGTAAG	20	7.029873E-4	45.000004	1
GTAACGG	80	0.0	45.000004	2
GCGTAAG	40	6.8030204E-9	45.000004	1
TCGCGCA	35	1.2102828E-7	45.000004	34
ACGGTTT	20	7.029873E-4	45.000004	17
ACCGAAC	45	3.8380676E-10	45.0	39
CGTTTTT	1010	0.0	42.549503	1
CGTAAGG	75	0.0	42.000004	2
CGAACGG	70	0.0	41.785717	2
TCGAACG	50	1.0786607E-9	40.5	1
TTTACGG	45	1.9250365E-8	40.0	2
CGTTAGG	90	0.0	40.0	2
CGGGCTC	45	1.9250365E-8	40.0	6
TCTCGCG	40	3.4535697E-7	39.375004	32
>>END_MODULE