##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936405.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540857
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03842420454944	33.0	31.0	34.0	30.0	34.0
2	32.14837378456782	33.0	31.0	34.0	30.0	34.0
3	32.08450477667849	33.0	31.0	34.0	30.0	34.0
4	35.72628439679989	37.0	35.0	37.0	33.0	37.0
5	32.17151853447399	37.0	35.0	37.0	0.0	37.0
6	33.90067060239583	37.0	35.0	37.0	17.0	37.0
7	35.526155712138326	37.0	35.0	37.0	32.0	37.0
8	35.75780289429554	37.0	35.0	37.0	35.0	37.0
9	37.67442410840573	39.0	37.0	39.0	35.0	39.0
10	37.248078512434894	39.0	37.0	39.0	34.0	39.0
11	37.24309198179925	39.0	37.0	39.0	34.0	39.0
12	37.17777712038487	39.0	37.0	39.0	34.0	39.0
13	37.133427135083764	39.0	37.0	39.0	33.0	39.0
14	38.27131016146597	40.0	38.0	41.0	33.0	41.0
15	38.43610972955883	40.0	38.0	41.0	34.0	41.0
16	38.39776133062898	40.0	38.0	41.0	34.0	41.0
17	38.387207709246624	40.0	37.0	41.0	34.0	41.0
18	38.294453062454586	40.0	37.0	41.0	34.0	41.0
19	38.160014569470306	40.0	37.0	41.0	34.0	41.0
20	38.2375526248158	40.0	37.0	41.0	34.0	41.0
21	38.13701958188579	40.0	37.0	41.0	34.0	41.0
22	38.21948500250528	40.0	37.0	41.0	34.0	41.0
23	38.226473910848895	40.0	37.0	41.0	34.0	41.0
24	38.19005393292497	40.0	37.0	41.0	34.0	41.0
25	37.91418064294259	40.0	36.0	41.0	34.0	41.0
26	37.96163126297709	40.0	36.0	41.0	34.0	41.0
27	37.943419425097574	40.0	36.0	41.0	34.0	41.0
28	37.879139957511875	40.0	36.0	41.0	33.0	41.0
29	37.84425088332036	40.0	36.0	41.0	33.0	41.0
30	37.662010475966845	40.0	36.0	41.0	33.0	41.0
31	37.6507893953485	40.0	36.0	41.0	33.0	41.0
32	37.594083094052586	40.0	36.0	41.0	33.0	41.0
33	37.49149035697051	40.0	36.0	41.0	33.0	41.0
34	37.399608399262654	40.0	35.0	41.0	33.0	41.0
35	37.3604945484666	40.0	35.0	41.0	33.0	41.0
36	37.245600962916264	40.0	35.0	41.0	33.0	41.0
37	37.19450982422341	40.0	35.0	41.0	32.0	41.0
38	37.03988669833246	39.0	35.0	41.0	32.0	41.0
39	36.93078762038765	39.0	35.0	41.0	32.0	41.0
40	36.85728390313891	39.0	35.0	41.0	31.0	41.0
41	36.76963781554089	39.0	35.0	41.0	31.0	41.0
42	36.756092645560656	39.0	35.0	41.0	31.0	41.0
43	36.6879452424578	39.0	35.0	41.0	31.0	41.0
44	36.60607332437225	39.0	35.0	41.0	31.0	41.0
45	36.548984297143235	39.0	35.0	41.0	31.0	41.0
46	36.410522559567504	39.0	35.0	41.0	31.0	41.0
47	36.330697393211146	39.0	35.0	41.0	31.0	41.0
48	36.295481060613064	38.0	35.0	41.0	31.0	41.0
49	36.26090815132281	38.0	35.0	41.0	31.0	41.0
50	36.11929216040469	38.0	35.0	40.0	30.0	41.0
51	35.0929302939594	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	25.0
10	31.0
11	28.0
12	12.0
13	13.0
14	33.0
15	26.0
16	44.0
17	78.0
18	153.0
19	248.0
20	435.0
21	724.0
22	1125.0
23	1634.0
24	2370.0
25	3239.0
26	4066.0
27	4956.0
28	5394.0
29	6221.0
30	7632.0
31	9840.0
32	13106.0
33	19637.0
34	33501.0
35	42551.0
36	43220.0
37	66632.0
38	106475.0
39	167283.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.005321184712415	19.205446171538874	22.39113111229031	12.398101531458408
2	35.29602094453802	23.507322637961607	23.97842683001237	17.218229587488004
3	33.78305171237499	24.5915278899968	25.29097339962319	16.334446998005017
4	28.898396433807825	26.02832171904958	25.417994035391978	19.65528781175061
5	23.204469943071828	36.71635940738495	22.09900213919761	17.980168510345617
6	25.70901365795395	35.17343771089216	24.343588046378247	14.773960584775644
7	80.7573905856816	5.66859632028429	9.250689184017217	4.323323910016881
8	81.015684367587	5.482964998141838	8.571211983944	4.930138650327167
9	75.39996708926758	6.538327136378006	11.236056850516865	6.825648923837539
10	38.51387705068068	26.12890283383593	18.798129635005186	16.559090480478204
11	30.68260187073478	25.995595878392997	24.154628672643604	19.167173578228628
12	26.287539959730577	23.766355986887476	29.851143647951307	20.09496040543064
13	25.511549263483698	24.609647281998754	31.111920526127978	18.766882928389574
14	22.178875377410296	26.950561793597938	30.372538397395243	20.49802443159652
15	20.75095635260337	25.191501635367576	33.57578805488327	20.48175395714579
16	24.866831713373404	24.52940426027582	29.698423058220563	20.905340968130208
17	24.430672063040692	23.96640886592944	28.088940329883872	23.513978741145998
18	25.797022133392005	22.94451213537035	29.359516471082003	21.898949260155643
19	24.97185023028268	25.721586297302245	26.72222047602231	22.584342996392763
20	26.233921350745206	25.634502280639797	27.367307809642842	20.764268558972148
21	25.4973125983393	27.064085331242826	27.643166308284815	19.79543576213306
22	24.21490338481336	24.10267408945433	27.467334249163827	24.215088276568483
23	22.351009601428842	27.345120799028212	27.829906980957997	22.47396261858495
24	21.958484405304915	25.80977966449542	29.30330937752493	22.928426552674736
25	22.72707943134692	26.79192466770329	27.268390720652597	23.212605180297192
26	21.683550365438553	25.563134063162725	28.23703862573656	24.516276945662163
27	22.806767777804485	24.779562804955837	28.95867114597759	23.454998271262088
28	21.05454861451363	25.777238715593953	29.16611969522443	24.00209297466798
29	22.61799329582496	25.249187862965627	27.93917800823508	24.193640832974335
30	23.621215959116736	23.9887807682992	29.64591379976593	22.74408947281814
31	23.80167031211577	24.7893620679773	27.344751015517964	24.06421660438896
32	24.45193461487972	24.536984822235823	26.835928905422318	24.175151657462138
33	23.43817312154599	23.80832641530016	27.88722342504581	24.866277038108038
34	21.83645584692442	24.20066671966897	30.05045695997278	23.91242047343383
35	23.03621844591084	24.312156448007514	30.016991552295707	22.634633553785935
36	24.258537839022146	25.67961586888956	27.985955622280933	22.07589066980736
37	22.835795783358634	25.30798344109441	28.790789432326847	23.065431343220112
38	22.66828385321813	26.720741341981334	28.041608040572648	22.569366764227883
39	24.471533140922645	23.710888460350887	28.18878187764973	23.628796521076737
40	24.300138483924584	23.30542084136842	30.49807989912306	21.896360775583933
41	21.913185925300034	24.141871141540186	28.926499980586367	25.018442952573416
42	23.571480076988927	23.944406747069927	29.196626834819554	23.287486341121593
43	24.25410043689922	23.438542905056234	28.27161338394437	24.035743274100177
44	23.225732494910854	24.136324388886525	28.292506152273152	24.34543696392947
45	23.0417651985645	23.320766857043544	28.356663591300475	25.280804353091487
46	23.633049031444543	23.942372937763587	28.495147515886828	23.929430514905047
47	21.348341613402432	24.439916650796793	30.785586578337714	23.426155157463064
48	22.169445897899077	24.34395782988849	29.125258617342475	24.361337654869956
49	24.101379847168474	23.021796889011327	29.67050440319715	23.20631886062305
50	22.00822028743272	23.73658841431284	30.12053093516401	24.13466036309043
51	22.153914990468827	23.10093056020353	27.783129366912142	26.962025082415497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	534.5
2	868.0
3	1998.5
4	3129.0
5	2129.5
6	1130.0
7	1095.0
8	1060.0
9	1125.5
10	1191.0
11	1221.0
12	1251.0
13	1245.0
14	1239.0
15	1233.5
16	1228.0
17	1253.5
18	1279.0
19	1297.5
20	1316.0
21	1417.0
22	1518.0
23	1678.0
24	1838.0
25	2193.5
26	3214.5
27	3880.0
28	4625.5
29	5371.0
30	6280.5
31	7190.0
32	8108.0
33	9026.0
34	10313.0
35	11600.0
36	12592.0
37	13584.0
38	15575.5
39	17567.0
40	20160.5
41	22754.0
42	25242.0
43	27730.0
44	30889.5
45	34049.0
46	40759.0
47	47469.0
48	50296.0
49	53123.0
50	53100.0
51	53077.0
52	48315.5
53	43554.0
54	39879.5
55	36205.0
56	32565.0
57	28925.0
58	27029.5
59	25134.0
60	22622.0
61	20110.0
62	18424.0
63	16738.0
64	14986.0
65	13234.0
66	11284.0
67	9334.0
68	8441.0
69	7548.0
70	6217.0
71	4886.0
72	4286.5
73	3687.0
74	3059.0
75	1933.5
76	1436.0
77	1117.0
78	798.0
79	558.5
80	319.0
81	236.5
82	154.0
83	128.0
84	102.0
85	63.5
86	25.0
87	17.0
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.130985877235354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91151630669464	25.982339800877636
2	9.928811271644909	7.174754796670749
3	3.8665020769332785	4.1910159580793245
4	2.1386942360163137	3.090925249489204
5	1.454110008808372	2.6269214096100932
6	1.1342410754716454	2.458874896754776
7	0.8912376741149362	2.254090707169479
8	0.852552607862732	2.464285298743083
9	0.7344875210381476	2.3883982424671424
>10	7.002156474261712	43.001403947982034
>50	0.051933786153542515	1.315744928518289
>100	0.030121595969045744	1.7165480042901593
>500	0.0015580135846058143	0.41393800803061775
>1k	0.0020773514461410857	0.9207587513174258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1611	0.29786061750148374	No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	1220	0.22556794124879587	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	1070	0.19783417798050132	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	1006	0.18600110565269562	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	753	0.13922349160683878	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	746	0.13792924932098502	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	707	0.13071847087122845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1447702442604977	0.0
2	0.0	0.0	0.0	0.6744851226849241	0.0
3	0.0	0.0	0.0	0.9438724098976254	0.0
4	0.0	0.0	0.0	1.3097731932839918	0.0
5	0.0	0.0	0.0	2.4022985742996763	0.0
6	0.0	0.0	0.0	3.2760970090060773	0.0
7	0.0	0.0	0.0	4.016958271779787	0.0
8	0.0	0.0	0.0	5.284391253140849	0.0
9	0.0	0.0	0.0	5.848680889773083	0.0
10	0.0	0.0	0.0	7.026626261655114	0.0
11	0.0	0.0	0.0	8.21437089655861	0.0
12	0.0	0.0	0.0	9.3263099118621	0.0
13	0.0	0.0	0.0	9.75747748480652	0.0
14	0.0	0.0	0.0	9.962522441236779	0.0
15	0.0	0.0	0.0	10.289226172537289	0.0
16	0.0	0.0	0.0	10.90787398517538	0.0
17	0.0	0.0	0.0	11.63671728386616	0.0
18	1.8489175512196383E-4	0.0	0.0	12.4822272800389	0.0
19	1.8489175512196383E-4	0.0	0.0	12.938355239924785	0.0
20	3.6978351024392767E-4	0.0	0.0	13.38228034397262	0.0
21	3.6978351024392767E-4	0.0	0.0	13.916247732764853	0.0
22	3.6978351024392767E-4	0.0	0.0	14.479612910621476	0.0
23	3.6978351024392767E-4	0.0	0.0	15.059248562928833	0.0
24	3.6978351024392767E-4	0.0	0.0	15.518519682651792	0.0
25	3.6978351024392767E-4	0.0	0.0	15.947838338044992	0.0
26	3.6978351024392767E-4	0.0	0.0	16.358482926170872	0.0
27	3.6978351024392767E-4	0.0	0.0	16.769312406051878	0.0
28	5.546752653658915E-4	0.0	0.0	17.15813976707337	0.0
29	5.546752653658915E-4	0.0	0.0	17.6037288969173	0.0
30	5.546752653658915E-4	0.0	0.0	18.148604899261727	0.0
31	5.546752653658915E-4	0.0	0.0	18.595488271391513	0.0
32	7.395670204878553E-4	0.0	0.0	19.041447184745692	0.0
33	7.395670204878553E-4	0.0	0.0	19.497760036386698	0.0
34	7.395670204878553E-4	0.0	0.0	19.947601676598435	0.0
35	7.395670204878553E-4	0.0	0.0	20.403174961218955	0.0
36	9.244587756098193E-4	0.0	0.0	20.825837513427764	0.0
37	9.244587756098193E-4	0.0	0.0	21.232784266451205	0.0
38	0.001109350530731783	0.0	0.0	21.68891222633709	0.0
39	0.001109350530731783	0.0	0.0	22.26041264141908	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.02997E-4	45.000004	38
GTTTACG	20	7.02997E-4	45.000004	1
GCGATAT	20	7.02997E-4	45.000004	9
CGGTCTA	45	3.8380676E-10	45.000004	31
TAGGCCG	20	7.02997E-4	45.000004	1
GCTAACG	25	3.8880564E-5	45.000004	1
CGTTATT	125	0.0	41.399998	1
CGTAAGG	60	3.6379788E-12	41.250004	2
CGACGGT	50	1.0786607E-9	40.500004	28
CGAATGG	50	1.0786607E-9	40.500004	2
GCGATAA	45	1.9250365E-8	40.000004	9
GGCGATA	175	0.0	38.571426	8
GACCGAT	230	0.0	38.152172	9
ACGGGTA	65	9.094947E-12	38.076927	5
ACGGGAG	315	0.0	37.857143	5
GTTGATC	125	0.0	37.8	16
ATAACGC	30	1.1391227E-4	37.500004	11
GTCGGTA	30	1.1391227E-4	37.500004	10
CGTTTTT	865	0.0	37.19653	1
CCACGGG	85	0.0	37.058823	3
>>END_MODULE