##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936404.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 203940 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.943341178778073 33.0 31.0 34.0 30.0 34.0 2 32.03998234774934 33.0 31.0 34.0 30.0 34.0 3 31.98891830930666 33.0 31.0 34.0 30.0 34.0 4 35.62463959988232 37.0 35.0 37.0 33.0 37.0 5 31.796802981269 37.0 35.0 37.0 0.0 37.0 6 33.67558595665392 37.0 35.0 37.0 17.0 37.0 7 35.370466803961946 37.0 35.0 37.0 32.0 37.0 8 35.659752868490735 37.0 35.0 37.0 33.0 37.0 9 37.54999019319408 39.0 37.0 39.0 35.0 39.0 10 37.185260370697264 39.0 37.0 39.0 34.0 39.0 11 37.18272531136609 39.0 37.0 39.0 34.0 39.0 12 36.922227125625184 39.0 35.0 39.0 33.0 39.0 13 36.70374619986271 39.0 35.0 39.0 33.0 39.0 14 37.81827988624105 40.0 36.0 41.0 33.0 41.0 15 38.03407374718054 40.0 36.0 41.0 33.0 41.0 16 38.02711091497499 40.0 36.0 41.0 34.0 41.0 17 38.03381877022654 40.0 36.0 41.0 34.0 41.0 18 37.98450524664117 40.0 36.0 41.0 34.0 41.0 19 37.86299892125135 40.0 36.0 41.0 34.0 41.0 20 37.923256840247134 40.0 36.0 41.0 34.0 41.0 21 37.82988133764833 40.0 35.0 41.0 34.0 41.0 22 37.88284789644013 40.0 35.0 41.0 34.0 41.0 23 37.89847013827596 40.0 35.0 41.0 34.0 41.0 24 37.89346866725508 40.0 35.0 41.0 34.0 41.0 25 37.58945278022948 39.0 35.0 41.0 33.0 41.0 26 37.6325831126802 39.0 35.0 41.0 33.0 41.0 27 37.57375698734922 39.0 35.0 41.0 33.0 41.0 28 37.42816024320879 39.0 35.0 41.0 33.0 41.0 29 37.346675492791995 39.0 35.0 41.0 33.0 41.0 30 37.214528782975385 39.0 35.0 41.0 33.0 41.0 31 37.19665587918015 39.0 35.0 41.0 33.0 41.0 32 37.13201922133961 39.0 35.0 41.0 32.0 41.0 33 37.043669706776505 39.0 35.0 41.0 32.0 41.0 34 36.95168677061881 39.0 35.0 41.0 32.0 41.0 35 36.94742081004217 39.0 35.0 41.0 32.0 41.0 36 36.82629204668039 39.0 35.0 41.0 32.0 41.0 37 36.732852799843094 39.0 35.0 41.0 31.0 41.0 38 36.60823771697558 39.0 35.0 41.0 31.0 41.0 39 36.51922624301265 39.0 35.0 41.0 31.0 41.0 40 36.428925174070805 39.0 35.0 41.0 31.0 41.0 41 36.300867902324214 39.0 35.0 41.0 30.0 41.0 42 36.31279788172992 39.0 35.0 41.0 31.0 41.0 43 36.20207904285574 38.0 35.0 41.0 30.0 41.0 44 36.14519956850054 38.0 35.0 41.0 30.0 41.0 45 36.11607825831127 38.0 35.0 40.0 30.0 41.0 46 35.95734529763656 38.0 35.0 40.0 30.0 41.0 47 35.87214376777484 38.0 35.0 40.0 30.0 41.0 48 35.87890556045896 38.0 35.0 40.0 30.0 41.0 49 35.78156810826714 38.0 35.0 40.0 30.0 41.0 50 35.59039423359812 37.0 35.0 40.0 29.0 41.0 51 34.468118073943316 36.0 34.0 39.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 7.0 10 11.0 11 7.0 12 8.0 13 6.0 14 7.0 15 8.0 16 15.0 17 32.0 18 41.0 19 102.0 20 195.0 21 351.0 22 547.0 23 756.0 24 1002.0 25 1369.0 26 1708.0 27 2049.0 28 2214.0 29 2670.0 30 3400.0 31 4206.0 32 5739.0 33 8798.0 34 15037.0 35 22294.0 36 15713.0 37 23533.0 38 37107.0 39 54946.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.282926350887514 19.5959595959596 22.594880847308033 12.526233205844855 2 32.03442188879082 25.009316465627148 24.94851426890262 18.007747376679415 3 31.63087182504658 25.258899676375407 26.19250760027459 16.917720898303422 4 28.045013239188 24.58223006766696 27.681180739433163 19.691575953711876 5 22.648327939590075 37.93272531136609 22.20702167304109 17.211925076002746 6 24.12376189075218 35.8237716975581 24.836716681376874 15.215749730312837 7 78.85995881141513 5.488378934980877 10.763459841129745 4.888202412474257 8 78.67804256153771 6.009120329508679 9.198293615769344 6.11454349318427 9 72.67970971854467 7.14670981661273 11.625968422084927 8.547612042757674 10 42.512503677552225 23.87564970089242 18.041090516818674 15.570756104736688 11 33.031774051191526 23.30195155437874 24.080611944689615 19.58566244974012 12 28.889379229185053 19.701382759635187 30.97675786996175 20.432480141218004 13 25.110816906933415 27.500245170148084 30.568794743552026 16.820143179366482 14 19.090909090909093 32.344316955967436 28.429930371677948 20.134843581445523 15 16.58919289987251 24.004609198783953 39.684220849269394 19.721977052074138 16 19.365009316465628 22.977836618613317 34.27919976463666 23.377954300284397 17 19.47239384132588 21.788271060115722 28.50642345787977 30.232911640678633 18 22.38354417966068 21.87947435520251 31.945670295184858 23.791311169951946 19 23.583897224673926 25.263312739040895 27.203589290967933 23.94920074531725 20 25.56830440325586 23.960478572129055 27.684613121506324 22.786603903108755 21 22.68510346180249 27.269785230950276 28.53927625772286 21.505835049524368 22 20.15494753358831 24.631264097283516 27.255075022065313 27.958713347062865 23 19.009512601745612 27.766499950965972 26.397960184367953 26.826027262920466 24 21.774541531823086 24.520447190350104 30.309404726880455 23.395606550946358 25 20.213788369128174 25.978228890850254 28.854074727861136 24.95390801216044 26 19.485142689026183 26.246935373148965 28.50397175639894 25.76395018142591 27 22.433068549573402 26.591644601353337 28.035206433264687 22.940080415808573 28 19.44493478474061 24.935765421202312 31.742179072276162 23.877120721780916 29 21.686770618809454 22.87339413553006 31.393056781406298 24.046778464254192 30 23.1303324507208 26.05276061586741 28.702559576345983 22.114347357065803 31 24.91517112876336 26.481318034716097 23.294596449936257 25.30891438658429 32 26.00372658625086 28.573600078454447 23.47308031774051 21.949593017554182 33 24.61655388839855 25.030891438658433 26.549965676179266 23.802588996763756 34 21.62008433853094 26.684809257624792 26.142492890065704 25.55261351377856 35 21.964793566735313 27.640482494851426 25.952240855153473 24.442483083259784 36 25.60655094635677 24.037952338923212 26.90546239089928 23.45003432382073 37 22.04079631264097 28.007747376679415 27.08934000196136 22.86211630871825 38 19.852407570854172 28.714327743453953 24.63322545846818 26.800039227223692 39 21.21849563597136 27.52819456702952 27.018730999313522 24.234578797685593 40 22.588996763754047 26.135137785623225 27.45758556438168 23.81827988624105 41 19.44640580562911 26.206237128567228 26.042953809944102 28.30440325585957 42 23.66872609591056 24.67490438364225 25.793370599195843 25.86299892125135 43 23.925664411101305 23.645189761694617 26.35284887712072 26.076296950083353 44 21.591154261057174 24.629793076395018 27.247229577326664 26.531823085221145 45 20.319701873099934 27.014808276944198 25.880160831617143 26.78532901833873 46 24.333137197214867 25.797783661861335 26.421496518583897 23.447582622339905 47 20.33539276257723 24.911248406394037 29.65921349416495 25.094145336863782 48 20.962538001372952 24.143375502598804 28.269098754535648 26.624987741492596 49 21.729430224575854 21.53476512699814 31.30038246543101 25.435422182994998 50 21.318525056389134 23.27988624105129 30.09757771893694 25.304010983622632 51 20.24517014808277 24.1590663920761 26.54359125232912 29.05217220751201 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 78.0 1 176.5 2 275.0 3 565.0 4 855.0 5 600.5 6 346.0 7 353.5 8 361.0 9 368.0 10 375.0 11 410.5 12 446.0 13 434.0 14 422.0 15 407.0 16 392.0 17 393.5 18 395.0 19 468.5 20 542.0 21 519.0 22 496.0 23 535.5 24 575.0 25 625.5 26 818.5 27 961.0 28 1170.0 29 1379.0 30 1536.0 31 1693.0 32 2205.0 33 2717.0 34 3243.5 35 3770.0 36 3940.5 37 4111.0 38 5169.0 39 6227.0 40 6723.0 41 7219.0 42 9658.0 43 12097.0 44 14629.5 45 17162.0 46 20671.0 47 24180.0 48 23527.5 49 22875.0 50 22336.0 51 21797.0 52 18846.5 53 15896.0 54 14440.5 55 12985.0 56 11582.0 57 10179.0 58 9467.5 59 8756.0 60 7566.0 61 6376.0 62 5778.0 63 5180.0 64 4506.5 65 3833.0 66 3128.0 67 2423.0 68 2124.0 69 1825.0 70 1608.5 71 1392.0 72 1227.0 73 1062.0 74 924.5 75 612.5 76 438.0 77 314.0 78 190.0 79 150.0 80 110.0 81 83.0 82 56.0 83 38.0 84 20.0 85 11.0 86 2.0 87 3.5 88 5.0 89 3.0 90 1.0 91 0.5 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 203940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.59615573207806 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5694739115521 29.16691183681475 2 10.002159744896016 7.720898303422576 3 3.5877174037325474 4.154162989114445 4 1.8650032396173442 2.8792782190840445 5 1.095117706096833 2.1133666764734724 6 0.8308665658785716 1.9240953221535746 7 0.5386657858295326 1.4553299990193194 8 0.46116905720783097 1.423948220064725 9 0.4103515302427807 1.4254192409532216 >10 5.548003506409361 42.5213298028832 >50 0.05462884148742901 1.3023438266156713 >100 0.030490516179030147 2.0770814945572225 >500 0.006352190870631281 1.8358340688437775 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC 919 0.45062273217613025 No Hit GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC 910 0.44620966951064034 Illumina PCR Primer Index 6 (95% over 23bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC 704 0.3451995685005394 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG 675 0.33097969991173876 No Hit CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC 536 0.26282239874472885 Illumina PCR Primer Index 6 (95% over 23bp) CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT 491 0.24075708541727958 Illumina PCR Primer Index 6 (95% over 24bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.20103952142787093 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 370 0.1814259095812494 No Hit GAATGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTT 306 0.1500441306266549 No Hit TCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC 252 0.1235657546337158 Illumina PCR Primer Index 6 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.271158183779543 0.0 2 4.903402961655388E-4 0.0 0.0 1.3141119937236443 0.0 3 4.903402961655388E-4 0.0 0.0 1.7691477885652642 0.0 4 4.903402961655388E-4 0.0 0.0 2.3678532901833873 0.0 5 4.903402961655388E-4 0.0 0.0 4.224281651466118 0.0 6 4.903402961655388E-4 0.0 0.0 5.326076296950084 0.0 7 4.903402961655388E-4 0.0 0.0 6.329802883200942 0.0 8 4.903402961655388E-4 0.0 0.0 8.1445523193096 0.0 9 4.903402961655388E-4 0.0 0.0 8.839854859272336 0.0 10 4.903402961655388E-4 0.0 0.0 10.57467882710601 0.0 11 4.903402961655388E-4 0.0 0.0 12.293321565166226 0.0 12 4.903402961655388E-4 0.0 0.0 14.08453466705894 0.0 13 4.903402961655388E-4 0.0 0.0 14.887712072178092 0.0 14 4.903402961655388E-4 0.0 0.0 15.256938315190743 0.0 15 9.806805923310777E-4 0.0 0.0 15.745807590467784 0.0 16 9.806805923310777E-4 0.0 0.0 16.609296852015298 0.0 17 9.806805923310777E-4 0.0 0.0 17.66745121114053 0.0 18 9.806805923310777E-4 0.0 0.0 18.915857605177994 0.0 19 9.806805923310777E-4 0.0 0.0 19.61508286751005 0.0 20 9.806805923310777E-4 0.0 0.0 20.201529861724037 0.0 21 9.806805923310777E-4 0.0 0.0 20.88261253309797 0.0 22 9.806805923310777E-4 0.0 0.0 21.559282141806413 0.0 23 9.806805923310777E-4 0.0 0.0 22.262920466803962 0.0 24 9.806805923310777E-4 0.0 0.0 22.82485044620967 0.0 25 9.806805923310777E-4 0.0 0.0 23.291164067863097 0.0 26 9.806805923310777E-4 0.0 0.0 23.79866627439443 0.0 27 9.806805923310777E-4 0.0 0.0 24.267431597528685 0.0 28 9.806805923310777E-4 0.0 0.0 24.728841816220456 0.0 29 9.806805923310777E-4 0.0 0.0 25.216240070609004 0.0 30 9.806805923310777E-4 0.0 0.0 25.821810336373442 0.0 31 9.806805923310777E-4 0.0 0.0 26.337157987643426 0.0 32 9.806805923310777E-4 0.0 0.0 26.80052956751986 0.0 33 9.806805923310777E-4 0.0 0.0 27.30263803079337 0.0 34 9.806805923310777E-4 0.0 0.0 27.82485044620967 0.0 35 9.806805923310777E-4 0.0 0.0 28.37354123761891 0.0 36 9.806805923310777E-4 0.0 0.0 28.853584387564972 0.0 37 9.806805923310777E-4 0.0 0.0 29.300284397371776 0.0 38 9.806805923310777E-4 0.0 0.0 29.739138962439934 0.0 39 9.806805923310777E-4 0.0 0.0 30.23536334215946 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATGA 25 3.8794875E-5 45.0 24 AGCGTTC 20 7.0196274E-4 45.0 12 ACTGCGA 20 7.0196274E-4 45.0 28 GAATGCG 20 7.0196274E-4 45.0 1 TTAGCGG 20 7.0196274E-4 45.0 2 TAGACGG 25 3.8794875E-5 45.0 2 TCCGAAT 20 7.0196274E-4 45.0 19 TTGTCGG 25 3.8794875E-5 45.0 2 GTTAGCG 20 7.0196274E-4 45.0 1 ATTCGCG 25 3.8794875E-5 45.0 1 TTCCGCG 20 7.0196274E-4 45.0 16 TACGGGT 20 7.0196274E-4 45.0 4 TCGATGG 20 7.0196274E-4 45.0 2 ACTTACA 25 3.8794875E-5 45.0 39 ACGGGTA 25 3.8794875E-5 45.0 5 CGTTATT 20 7.0196274E-4 45.0 1 CGTTAGC 20 7.0196274E-4 45.0 42 TAGCAAG 20 7.0196274E-4 45.0 1 GCCGATA 20 7.0196274E-4 45.0 9 CTACGGG 40 6.7684596E-9 45.0 3 >>END_MODULE