FastQCFastQC Report
Sat 14 Jan 2017
SRR2936403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839169
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127711.5218626998852436No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT36230.4317366346945609No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC28930.34474581401362536No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC23170.27610648153113376No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG23170.27610648153113376No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT20840.2483409182179037No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT15630.18625568866342776No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.18303821995331096No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC13520.1611117665214039No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC12650.15074436734436092No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC8700.10367399177043005No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG8610.1026015022003911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54100.043.7523081
CGTTATT6650.043.6466181
TCACGAC950.042.6315825
GCGATAT501.0804797E-940.5000049
CGGTCTA950.040.26315731
ACTACGG750.039.0000042
ATAACGG356.246275E-638.5714262
CGTTTCT4500.038.01
CGTTTAC301.1396594E-437.49999633
TGCCGGA301.1396594E-437.49999616
CTCACGA1100.036.8181824
ACCGGTA552.746674E-936.8181841
CGACGGT1100.036.8181828
CGTTCTG4350.036.724141
AAGGGCG3200.036.56255
ATGGGCG2100.036.428575
GTTTTTT66300.036.2104072
GTTATTT8350.036.1077842
GCGACGT250.002106784836.00000417
TACTTCG250.002106784836.00000429
Produced by FastQC (version 0.11.5)