##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936403.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 839169 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.982968865627782 33.0 31.0 34.0 30.0 34.0 2 32.13205325744874 33.0 31.0 34.0 30.0 34.0 3 31.993326731564203 33.0 31.0 34.0 30.0 34.0 4 35.69490174208056 37.0 35.0 37.0 33.0 37.0 5 32.21229335211382 37.0 35.0 37.0 0.0 37.0 6 33.91424730894492 37.0 35.0 37.0 18.0 37.0 7 35.501353124340866 37.0 35.0 37.0 32.0 37.0 8 35.73753558579976 37.0 35.0 37.0 35.0 37.0 9 37.64608320850746 39.0 37.0 39.0 35.0 39.0 10 37.31772026850372 39.0 37.0 39.0 34.0 39.0 11 37.2986049294004 39.0 37.0 39.0 34.0 39.0 12 37.239498837540474 39.0 37.0 39.0 34.0 39.0 13 37.225185868400764 39.0 37.0 39.0 34.0 39.0 14 38.41272854454824 40.0 38.0 41.0 34.0 41.0 15 38.545726784473686 40.0 38.0 41.0 34.0 41.0 16 38.47122093404308 40.0 38.0 41.0 34.0 41.0 17 38.4712686002462 40.0 38.0 41.0 34.0 41.0 18 38.30489329324605 40.0 38.0 41.0 34.0 41.0 19 38.081763029854535 40.0 37.0 41.0 34.0 41.0 20 38.07686532748469 40.0 36.0 41.0 34.0 41.0 21 37.97195558939856 40.0 36.0 41.0 34.0 41.0 22 38.038011413672336 40.0 36.0 41.0 34.0 41.0 23 38.046028869035915 40.0 36.0 41.0 34.0 41.0 24 37.98092398551424 40.0 36.0 41.0 34.0 41.0 25 37.687965117872565 40.0 35.0 41.0 33.0 41.0 26 37.705278674498224 40.0 35.0 41.0 33.0 41.0 27 37.66761164914338 40.0 35.0 41.0 33.0 41.0 28 37.52445931629982 40.0 35.0 41.0 33.0 41.0 29 37.417957527029714 39.0 35.0 41.0 33.0 41.0 30 37.22213880636677 39.0 35.0 41.0 33.0 41.0 31 37.103672800115355 39.0 35.0 41.0 32.0 41.0 32 36.93774555542447 39.0 35.0 41.0 32.0 41.0 33 36.74563764867386 39.0 35.0 41.0 31.0 41.0 34 36.554499749156605 39.0 35.0 41.0 31.0 41.0 35 36.442142166834095 39.0 35.0 41.0 30.0 41.0 36 36.33563203597845 39.0 35.0 41.0 30.0 41.0 37 36.28445521700635 39.0 35.0 41.0 30.0 41.0 38 36.156653784875274 39.0 35.0 41.0 30.0 41.0 39 36.05093372133623 39.0 35.0 41.0 29.0 41.0 40 35.97214267924578 39.0 35.0 41.0 29.0 41.0 41 35.812928027608265 39.0 35.0 41.0 28.0 41.0 42 35.782831586962814 39.0 35.0 41.0 28.0 41.0 43 35.70292873068476 39.0 35.0 41.0 28.0 41.0 44 35.63536546273754 39.0 35.0 41.0 27.0 41.0 45 35.59855642903873 38.0 35.0 41.0 27.0 41.0 46 35.488530915703514 38.0 35.0 40.0 27.0 41.0 47 35.403107121449914 38.0 35.0 40.0 27.0 41.0 48 35.34516408494594 38.0 35.0 40.0 27.0 41.0 49 35.3079963630687 38.0 35.0 40.0 27.0 41.0 50 35.20036369312975 38.0 35.0 40.0 27.0 41.0 51 34.18179532370714 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 18.0 9 32.0 10 55.0 11 36.0 12 36.0 13 33.0 14 35.0 15 52.0 16 73.0 17 148.0 18 295.0 19 524.0 20 886.0 21 1511.0 22 2222.0 23 3368.0 24 5422.0 25 8422.0 26 11666.0 27 13245.0 28 12962.0 29 12587.0 30 13912.0 31 16710.0 32 21408.0 33 30795.0 34 51090.0 35 63954.0 36 68519.0 37 100717.0 38 158053.0 39 240209.0 40 168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.161397763740084 18.585886752251334 21.794775545807816 19.45793993820077 2 39.029682936333444 21.800257159165795 22.60784180540511 16.562218099095652 3 30.364086375926664 22.032987395864243 31.15665616818543 16.446270060023664 4 26.76207057219702 23.66436319740124 30.97075797604535 18.602808254356393 5 21.083119133333096 34.785245880150484 26.461535161570556 17.67009982494587 6 23.496935658967384 31.952562594662098 29.331398085486953 15.219103660883565 7 74.178026118696 5.281653635918391 15.225896094827144 5.3144241505584695 8 75.34417977785166 4.660682174865849 14.523534591959427 5.471603455323064 9 69.14924169029123 6.10306148106043 17.090359629585937 7.657337199062406 10 37.40736371338789 22.149292931459573 23.525058718803958 16.91828463634858 11 27.509119140483023 21.80395128990704 30.166033301992805 20.520896267617132 12 23.561761695200847 19.26155518137586 35.00474874548512 22.171934377938175 13 22.84641115198488 19.785883415617118 37.40509956874003 19.962605863657977 14 19.213412316231892 23.496578162444038 36.09296816255129 21.197041358772786 15 18.12328625104121 22.33125866184285 37.681444381286724 21.86401070582922 16 21.60637487800431 21.91155774343428 35.03168015024387 21.45038722831754 17 21.86353404379809 22.941386061687215 32.50596721280219 22.689112681712505 18 22.58400870384869 22.882518300842857 32.62072359679635 21.912749398512098 19 22.142381332008213 25.53454667653357 30.39340109084106 21.929670900617158 20 22.6185667011055 25.325172879360412 29.948556250290466 22.107704169243622 21 22.55636230604324 25.437188456675592 31.24364698886637 20.7628022484148 22 21.78571896721638 22.272748397521834 31.616039200685442 24.325493434576348 23 21.46409126171248 24.516277412535498 31.26021099444808 22.759420331303946 24 21.70385226337007 23.57200992887011 32.099136169234086 22.62500163852573 25 22.827821332770874 24.30690361536234 29.91256826694027 22.952706784926516 26 20.84157064905877 23.073183113294224 30.892823733955854 25.19242250369115 27 21.904526978475133 21.71553048313272 31.25329939499672 25.126643143395434 28 21.167130816319478 22.57673960787398 31.401660452185435 24.854469123621108 29 21.248640023642437 22.832707118589937 30.017433913788523 25.901218943979103 30 22.154893710325332 21.685858271694975 31.856038533358593 24.303209484621096 31 22.892647369004337 22.05253053914051 30.246350854237942 24.808471237617212 32 21.93265003831171 22.19088169367553 29.918884038852724 25.957584229160034 33 21.21646533654127 21.51974155384672 30.82978518033912 26.43400792927289 34 20.304491705484832 21.765937492924547 31.32765867185275 26.60191212973787 35 20.130986726154088 22.699360915381764 31.50557277497143 25.664079583492715 36 21.638311234089915 24.391630291395415 28.526673411434405 25.44338506308026 37 20.272436183891447 24.678104172103595 30.351335666593975 24.698123977410987 38 19.96344002221245 25.27405087652189 29.260256277341036 25.50225282392462 39 20.57738071830585 24.330855882426544 29.081031353636753 26.010732045630856 40 22.21185482304518 22.402400469988763 30.55677700201032 24.828967704955737 41 20.315931594231913 24.26817482533316 29.76849716803171 25.64739641240322 42 20.93332809005099 25.028808261506324 29.731079198588127 24.30678444985456 43 21.038551233422588 23.124185950624966 30.90676609836636 24.930496717586088 44 22.549212375576314 22.653005532854525 29.60500209135466 25.192780000214498 45 21.99783357106852 21.91394105358992 29.736441646438323 26.35178372890324 46 22.920889594348694 21.966373877014046 29.048260838996676 26.064475689640588 47 20.958352846685234 23.360610318064655 30.991731105415 24.689305729835112 48 20.28625938279417 23.337968871586057 30.91367769781772 25.462094047802054 49 21.995211929897316 21.851736658527663 31.17107519462707 24.981976216947956 50 20.891977658850603 21.388301998763062 32.983940064516204 24.73578027787013 51 20.266835405025684 21.131381163984848 31.279754137724343 27.32202929326512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 361.0 1 795.0 2 1229.0 3 10872.5 4 20516.0 5 12865.0 6 5214.0 7 5075.0 8 4936.0 9 5024.0 10 5112.0 11 5047.0 12 4982.0 13 4818.0 14 4654.0 15 4450.0 16 4246.0 17 3978.5 18 3711.0 19 3599.5 20 3488.0 21 3339.0 22 3190.0 23 3362.0 24 3534.0 25 3852.0 26 4990.0 27 5810.0 28 6557.0 29 7304.0 30 8190.5 31 9077.0 32 10520.0 33 11963.0 34 13875.5 35 15788.0 36 17580.0 37 19372.0 38 20990.0 39 22608.0 40 25130.0 41 27652.0 42 30529.5 43 33407.0 44 39061.5 45 44716.0 46 57334.0 47 69952.0 48 74657.5 49 79363.0 50 79117.0 51 78871.0 52 70035.5 53 61200.0 54 55226.5 55 49253.0 56 47205.0 57 45157.0 58 44033.0 59 42909.0 60 39825.0 61 36741.0 62 33698.0 63 30655.0 64 26978.0 65 23301.0 66 20234.5 67 17168.0 68 15150.0 69 13132.0 70 11227.0 71 9322.0 72 7889.5 73 6457.0 74 5443.5 75 3286.0 76 2142.0 77 1664.5 78 1187.0 79 870.0 80 553.0 81 364.0 82 175.0 83 126.0 84 77.0 85 57.0 86 37.0 87 31.5 88 26.0 89 16.5 90 7.0 91 7.0 92 7.0 93 4.5 94 2.0 95 1.5 96 1.0 97 2.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 839169.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.34161947190082 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4291838646606 27.770522063991553 2 9.390767187857728 7.201144441321063 3 3.9752021450904853 4.572470639128298 4 2.321589873536806 3.5605406200386622 5 1.713936343082649 3.2857547532768088 6 1.3625793962145818 3.1346100425947125 7 1.1413688325387388 3.0633350618001605 8 1.0066788848395107 3.087815898633119 9 0.8753916290617866 3.0207539457336936 >10 5.702463369333137 33.50042589446679 >50 0.0482524695249604 1.2590133549858042 >100 0.026319464978506502 1.909419844990858 >500 0.00313326964026406 0.8234027694405689 >1k 0.0028199426762376537 2.2765512811349256 >5k 0.0 0.0 >10k+ 3.13326964026406E-4 1.534239388463015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12771 1.5218626998852436 No Hit CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT 3623 0.4317366346945609 No Hit CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC 2893 0.34474581401362536 No Hit GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC 2317 0.27610648153113376 No Hit CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG 2317 0.27610648153113376 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT 2084 0.2483409182179037 No Hit CGTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT 1563 0.18625568866342776 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1536 0.18303821995331096 No Hit CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC 1352 0.1611117665214039 No Hit GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC 1265 0.15074436734436092 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC 870 0.10367399177043005 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG 861 0.1026015022003911 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.958275389105174E-4 0.0 0.0 0.10057568856809534 0.0 2 5.958275389105174E-4 0.0 0.0 0.4890552439377527 0.0 3 5.958275389105174E-4 0.0 0.0 0.989192880099241 0.0 4 5.958275389105174E-4 0.0 0.0 1.5481982771050884 0.0 5 7.149930466926209E-4 0.0 0.0 2.9841426458794356 0.0 6 7.149930466926209E-4 0.0 0.0 4.9130747203483445 0.0 7 8.341585544747244E-4 0.0 0.0 6.260598282348371 0.0 8 8.341585544747244E-4 0.0 0.0 8.083353889383426 0.0 9 8.341585544747244E-4 0.0 0.0 9.15429430782119 0.0 10 8.341585544747244E-4 0.0 0.0 10.587736200932113 0.0 11 8.341585544747244E-4 0.0 0.0 11.837663212058596 0.0 12 8.341585544747244E-4 0.0 0.0 12.926835953187021 0.0 13 8.341585544747244E-4 0.0 0.0 13.469754006642285 0.0 14 9.533240622568279E-4 0.0 0.0 13.799961628706495 0.0 15 9.533240622568279E-4 0.0 0.0 14.103476177027511 0.0 16 9.533240622568279E-4 0.0 0.0 14.546771865976936 0.0 17 9.533240622568279E-4 0.0 0.0 15.028081351908853 0.0 18 9.533240622568279E-4 0.0 0.0 15.617473953399136 0.0 19 9.533240622568279E-4 0.0 0.0 15.961981436397197 0.0 20 0.0010724895700389315 0.0 0.0 16.31208969826102 0.0 21 0.0010724895700389315 0.0 0.0 16.7110558183155 0.0 22 0.0010724895700389315 0.0 0.0 17.163884747887494 0.0 23 0.0010724895700389315 0.0 0.0 17.600268837385556 0.0 24 0.0010724895700389315 0.0 0.0 17.984339268967275 0.0 25 0.0010724895700389315 0.0 0.0 18.320028504389462 0.0 26 0.0010724895700389315 0.0 0.0 18.63879623770659 0.0 27 0.0010724895700389315 0.0 0.0 18.994862774959515 0.0 28 0.0011916550778210348 0.0 0.0 19.330790341397265 0.0 29 0.0011916550778210348 0.0 0.0 19.70556586337198 0.0 30 0.0011916550778210348 0.0 0.0 20.200698548206617 0.0 31 0.0013108205856031383 0.0 0.0 20.586318131389504 0.0 32 0.0014299860933852419 0.0 0.0 20.988620885661888 0.0 33 0.0014299860933852419 0.0 0.0 21.386752847161894 0.0 34 0.0015491516011673454 0.0 0.0 21.787148953309764 0.0 35 0.0015491516011673454 0.0 0.0 22.207564864765022 0.0 36 0.0015491516011673454 0.0 0.0 22.594137772010168 0.0 37 0.0015491516011673454 0.0 0.0 22.975467396912897 0.0 38 0.0015491516011673454 0.0 0.0 23.40768069363859 0.0 39 0.0015491516011673454 0.0 0.0 23.928076466123034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 5410 0.0 43.752308 1 CGTTATT 665 0.0 43.646618 1 TCACGAC 95 0.0 42.63158 25 GCGATAT 50 1.0804797E-9 40.500004 9 CGGTCTA 95 0.0 40.263157 31 ACTACGG 75 0.0 39.000004 2 ATAACGG 35 6.246275E-6 38.571426 2 CGTTTCT 450 0.0 38.0 1 CGTTTAC 30 1.1396594E-4 37.499996 33 TGCCGGA 30 1.1396594E-4 37.499996 16 CTCACGA 110 0.0 36.81818 24 ACCGGTA 55 2.746674E-9 36.81818 41 CGACGGT 110 0.0 36.81818 28 CGTTCTG 435 0.0 36.72414 1 AAGGGCG 320 0.0 36.5625 5 ATGGGCG 210 0.0 36.42857 5 GTTTTTT 6630 0.0 36.210407 2 GTTATTT 835 0.0 36.107784 2 GCGACGT 25 0.0021067848 36.000004 17 TACTTCG 25 0.0021067848 36.000004 29 >>END_MODULE