##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936402.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191510
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92530938332202	33.0	31.0	34.0	30.0	34.0
2	32.01979531094982	33.0	31.0	34.0	30.0	34.0
3	31.981045376220564	33.0	31.0	34.0	30.0	34.0
4	35.65176753172158	37.0	35.0	37.0	33.0	37.0
5	32.27601169651715	37.0	35.0	37.0	16.0	37.0
6	33.93631664142865	37.0	35.0	37.0	19.0	37.0
7	35.47640332097541	37.0	35.0	37.0	32.0	37.0
8	35.648890397368284	37.0	35.0	37.0	33.0	37.0
9	37.54498981776408	39.0	37.0	39.0	35.0	39.0
10	37.27569317529111	39.0	37.0	39.0	34.0	39.0
11	37.219737872695944	39.0	37.0	39.0	34.0	39.0
12	36.98934259307608	39.0	35.0	39.0	33.0	39.0
13	36.769186987624664	39.0	35.0	39.0	33.0	39.0
14	37.907952587332254	40.0	36.0	41.0	33.0	41.0
15	38.04772596731241	40.0	36.0	41.0	33.0	41.0
16	38.055798652811866	40.0	36.0	41.0	34.0	41.0
17	38.01467286303587	40.0	36.0	41.0	34.0	41.0
18	37.973896924442585	40.0	36.0	41.0	34.0	41.0
19	37.84338676831497	40.0	36.0	41.0	34.0	41.0
20	37.85126103075557	40.0	36.0	41.0	34.0	41.0
21	37.855031068873686	40.0	35.0	41.0	34.0	41.0
22	37.912474544410216	40.0	35.0	41.0	34.0	41.0
23	37.9042138791708	40.0	35.0	41.0	34.0	41.0
24	37.8870816145371	40.0	35.0	41.0	34.0	41.0
25	37.68250221920526	40.0	35.0	41.0	33.0	41.0
26	37.686658660122184	40.0	35.0	41.0	33.0	41.0
27	37.63394078638191	40.0	35.0	41.0	33.0	41.0
28	37.58381807738499	40.0	35.0	41.0	33.0	41.0
29	37.57388125946426	40.0	35.0	41.0	33.0	41.0
30	37.4236332306407	39.0	35.0	41.0	33.0	41.0
31	37.40356639339982	39.0	35.0	41.0	33.0	41.0
32	37.305947470106	39.0	35.0	41.0	33.0	41.0
33	37.18908151010391	39.0	35.0	41.0	33.0	41.0
34	37.069651715315125	39.0	35.0	41.0	32.0	41.0
35	37.04516735418516	39.0	35.0	41.0	32.0	41.0
36	36.93944963709467	39.0	35.0	41.0	32.0	41.0
37	36.852065166309856	39.0	35.0	41.0	32.0	41.0
38	36.73647329121195	39.0	35.0	41.0	31.0	41.0
39	36.666315074930814	39.0	35.0	41.0	31.0	41.0
40	36.585196595478045	39.0	35.0	41.0	31.0	41.0
41	36.464826901989454	39.0	35.0	41.0	31.0	41.0
42	36.509378100360294	39.0	35.0	41.0	31.0	41.0
43	36.39617252362801	39.0	35.0	41.0	31.0	41.0
44	36.3512401441178	39.0	35.0	41.0	31.0	41.0
45	36.33827998537935	39.0	35.0	41.0	31.0	41.0
46	36.24260874105791	38.0	35.0	40.0	31.0	41.0
47	36.11816615320349	38.0	35.0	40.0	31.0	41.0
48	36.10213565871234	38.0	35.0	40.0	31.0	41.0
49	36.10055871756044	38.0	35.0	40.0	31.0	41.0
50	35.9484100046995	38.0	35.0	40.0	30.0	41.0
51	34.84273406088455	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	9.0
10	16.0
11	10.0
12	4.0
13	3.0
14	5.0
15	9.0
16	8.0
17	22.0
18	44.0
19	96.0
20	230.0
21	360.0
22	550.0
23	710.0
24	957.0
25	1185.0
26	1520.0
27	1746.0
28	1952.0
29	2323.0
30	2916.0
31	3719.0
32	5130.0
33	7872.0
34	13975.0
35	19605.0
36	14435.0
37	21261.0
38	34911.0
39	55887.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.71609837606391	18.97185525560023	22.75546968826693	11.556576680068927
2	32.2949193253616	27.7698292517362	23.511565975667068	16.42368544723513
3	29.94778340556629	27.938488851757086	26.064957443475535	16.048770299201088
4	26.92653125163177	24.357996971437522	30.6542739282544	18.061197848676308
5	24.705759490366038	34.12563312620751	22.859380711190017	18.309226672236438
6	23.170591614014935	36.25711451099159	24.91514803404522	15.657145840948253
7	75.39031904339198	5.336535951125267	14.376794945433657	4.896350060049084
8	73.73400866795467	8.58127512923607	10.810401545611196	6.874314657198058
9	68.30609367657041	6.376168346300454	13.645240457417367	11.672497519711765
10	42.0641219779646	22.240091901206203	19.572346091587907	16.123440029241294
11	34.24416479557203	20.8526969871025	25.91300715367344	18.99013106365203
12	30.19946739073678	18.237167771917914	30.863140305989244	20.700224531356064
13	24.387238264320402	23.591457365150646	33.704767375071796	18.316536995457156
14	18.826170957130177	30.43966372513185	29.53057281604094	21.203592501697038
15	16.885802307973474	22.412928828781787	41.24171061563365	19.459558247611092
16	18.253354916192364	21.74716724975197	35.57098846013263	24.428489373923032
17	19.182810297112425	22.414495326614798	30.946686857083183	27.456007519189598
18	22.3596679024594	21.804083337684716	32.382643204010236	23.453605555845648
19	22.486032060988983	25.727116077489427	27.827267505613285	23.95958435590831
20	25.34697927001201	24.861887107722836	28.8334812803509	20.957652341914258
21	22.242180564983553	29.391676674847268	28.390684559553026	19.975458200616156
22	19.643360660017752	24.164273406088455	28.767688371364418	27.42467756252937
23	19.756670669938906	27.567751031277737	28.31079317006945	24.364785128713905
24	22.99775468643935	22.735105216437784	29.61098637146885	24.65615372565401
25	19.082032269855357	26.920787426244058	28.06119784867631	25.93598245522427
26	17.67636154769986	25.665500496057646	31.08976032583155	25.568377630410943
27	20.627643465093207	26.894156963082867	27.739543626964647	24.738655944859275
28	19.06218996397055	25.43522531460498	32.26045637303535	23.24212834838912
29	21.894418046055037	22.51527335387186	32.25210171792596	23.338206882147148
30	21.020312255234714	28.766121873531407	29.305519294031647	20.908046577202235
31	25.700485614328233	25.34906793378936	25.416949506553184	23.533496945329226
32	28.40217221032844	26.36781369119106	24.021199937340086	21.20881416114041
33	25.68482063599812	26.5808573964806	24.418568220980628	23.31575374654065
34	23.197744243120464	22.90219831862566	31.421335700485614	22.47872173776826
35	21.378518093049973	25.134457730666803	27.443997702469847	26.04302647381338
36	26.49261135188763	26.434650932066212	27.42519972847371	19.64753798757245
37	24.6404887473239	26.04041564409169	28.606861260508587	20.71223434807582
38	24.388804762153413	27.002767479504985	23.491201503837917	25.117226254503684
39	25.745914051485563	24.15435225314605	28.477364106312987	21.622369589055403
40	23.562738238212102	23.219675212782622	29.1332045324004	24.084382016604877
41	20.418254921414025	24.254608114458776	27.956242493864554	27.37089447026265
42	23.730875672288654	22.04375750613545	28.44760064748577	25.77776617409013
43	26.77458096182967	22.72152890188502	26.53542895932327	23.968461176962037
44	23.247872173776827	23.060414599759802	28.83243694846222	24.85927627800115
45	22.373766382956504	22.90794214401337	27.44295337058117	27.27533810244896
46	25.77567751031278	25.472821262597257	27.209023027518146	21.542478199571825
47	20.022453135606497	23.655161610359773	32.09858493029085	24.223800323742886
48	21.78163020207822	22.45052477677406	29.44545976711399	26.32238525403373
49	23.275546968826692	19.00631820792648	33.10062137747376	24.617513445773067
50	22.436426296276956	20.78220458461699	30.665239413085477	26.116129706020573
51	19.99477834055663	21.763354394026422	27.45078585974623	30.791081405670724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	187.5
2	238.0
3	692.0
4	1146.0
5	798.0
6	450.0
7	428.0
8	406.0
9	410.0
10	414.0
11	429.5
12	445.0
13	453.0
14	461.0
15	446.0
16	431.0
17	441.5
18	452.0
19	441.5
20	431.0
21	452.0
22	473.0
23	484.5
24	496.0
25	555.5
26	809.0
27	1003.0
28	1300.0
29	1597.0
30	1777.0
31	1957.0
32	2299.0
33	2641.0
34	2942.0
35	3243.0
36	3483.0
37	3723.0
38	4361.0
39	4999.0
40	6093.0
41	7187.0
42	8824.0
43	10461.0
44	12317.0
45	14173.0
46	19587.5
47	25002.0
48	23308.0
49	21614.0
50	21672.0
51	21730.0
52	18406.0
53	15082.0
54	13042.0
55	11002.0
56	9774.5
57	8547.0
58	8387.5
59	8228.0
60	7348.5
61	6469.0
62	6170.5
63	5872.0
64	4882.0
65	3892.0
66	3285.5
67	2679.0
68	2086.0
69	1493.0
70	1210.5
71	928.0
72	794.0
73	660.0
74	507.5
75	289.5
76	224.0
77	155.5
78	87.0
79	65.0
80	43.0
81	27.5
82	12.0
83	8.0
84	4.0
85	3.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	191510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.19215706751606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24128254430182	29.839172889144173
2	10.95723120095619	8.807895149078378
3	3.807878189471496	4.591405148556211
4	1.9227771137556513	3.0912223904756932
5	1.1666580055084965	2.3445250900736254
6	0.8678480486410644	2.092841104903138
7	0.7210414176583693	2.028614693749674
8	0.6534843839318193	2.1011957600125317
9	0.5573455282440367	2.0160827110855832
>10	5.005716364392247	33.97316067046107
>50	0.059761991373486464	1.6724975197117644
>100	0.029880995686743232	2.4855098950446455
>500	0.0038975211765317256	1.0380658973421755
>1k	0.005196694902042301	3.9178110803613384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	2026	1.0579082032269855	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	2024	1.0568638713383114	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	2007	1.0479870502845803	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	1446	0.7550519555114615	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	854	0.44592971646389223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.3138217325466033	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	533	0.2783144483316798	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	472	0.2464623257271161	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT	358	0.1869354080726855	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTA	344	0.17962508485196596	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	344	0.17962508485196596	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTC	323	0.16865960002088665	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	299	0.15612761735679598	No Hit
GGGACAGTTCAATGGTCTGAAAGTTTCAAAAAGGCTGGGGAATTTGAGGGG	239	0.12479766069656936	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	212	0.1106991801994674	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	210	0.10965484831079317	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	209	0.10913268236645607	No Hit
ACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	197	0.10286669103441073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2913685969401076	0.0
2	0.0	0.0	0.0	1.8098271630724245	0.0
3	0.0	0.0	0.0	2.3314709414651977	0.0
4	0.0	0.0	0.0	3.126729674690617	0.0
5	0.0	0.0	0.0	5.878022035402851	0.0
6	0.0	0.0	0.0	7.079003707378205	0.0
7	0.0	0.0	0.0	8.440812490209389	0.0
8	0.0	0.0	0.0	10.508067463840009	0.0
9	0.0	0.0	0.0	11.305414860842776	0.0
10	0.0	0.0	0.0	13.994047308234556	0.0
11	0.0	0.0	0.0	16.205942248446558	0.0
12	0.0	0.0	0.0	19.03660383269803	0.0
13	0.0	0.0	0.0	19.815675421649	0.0
14	0.0	0.0	0.0	20.163437940577516	0.0
15	0.0	0.0	0.0	20.85478565087985	0.0
16	0.0	0.0	0.0	22.03070335752702	0.0
17	0.0	0.0	0.0	23.247872173776827	0.0
18	0.0	0.0	0.0	24.641533079212575	0.0
19	0.0	0.0	0.0	25.648269019894524	0.0
20	0.0	0.0	0.0	26.479557203279203	0.0
21	0.0	0.0	0.0	27.371416636206988	0.0
22	0.0	0.0	0.0	28.27998537935356	0.0
23	0.0	0.0	0.0	29.213095921883976	0.0
24	0.0	0.0	0.0	29.877813169025117	0.0
25	0.0	0.0	0.0	30.452195707795937	0.0
26	0.0	0.0	0.0	31.010913268236646	0.0
27	0.0	0.0	0.0	31.576941151898072	0.0
28	0.0	0.0	0.0	32.13461438045011	0.0
29	0.0	0.0	0.0	32.65834682262023	0.0
30	0.0	0.0	0.0	33.31940890815101	0.0
31	0.0	0.0	0.0	33.94496370946687	0.0
32	0.0	0.0	0.0	34.50315910396324	0.0
33	0.0	0.0	0.0	35.12505874366874	0.0
34	0.0	0.0	0.0	35.660801002558614	0.0
35	0.0	0.0	0.0	36.31037543731398	0.0
36	0.0	0.0	0.0	36.88371364419613	0.0
37	0.0	0.0	0.0	37.4560075191896	0.0
38	0.0	0.0	0.0	38.00428176074357	0.0
39	5.221659443371104E-4	0.0	0.0	38.58127512923607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTG	20	7.018553E-4	45.0	42
CAAACGG	20	7.018553E-4	45.0	2
CGTATGG	25	3.878598E-5	45.0	2
CTCGTCC	25	3.878598E-5	45.0	37
GATCGGA	20	7.018553E-4	45.0	34
CCTAGTA	20	7.018553E-4	45.0	37
GGCGTAG	35	1.204844E-7	45.0	1
GGCCGAT	25	3.878598E-5	45.0	8
CCCAGTG	20	7.018553E-4	45.0	31
GCAAACG	20	7.018553E-4	45.0	1
GTGATCG	20	7.018553E-4	45.0	19
TGTATCC	20	7.018553E-4	45.0	21
TTGTAGT	20	7.018553E-4	45.0	42
TATGCGG	55	1.8189894E-12	45.0	2
GCGCAAG	25	3.878598E-5	45.0	1
ACGATGG	45	3.8198777E-10	45.0	2
GTAGCGG	25	3.878598E-5	45.0	2
TCGTCCC	25	3.878598E-5	45.0	38
GTCAAGC	20	7.018553E-4	45.0	16
ATCCAAT	20	7.018553E-4	45.0	24
>>END_MODULE