##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783719
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.017201318329654	33.0	31.0	34.0	30.0	34.0
2	32.12721779107052	33.0	31.0	34.0	30.0	34.0
3	32.07121812792595	33.0	31.0	34.0	30.0	34.0
4	35.728110457957506	37.0	35.0	37.0	33.0	37.0
5	32.13012572108115	37.0	35.0	37.0	0.0	37.0
6	33.87485055230255	37.0	35.0	37.0	17.0	37.0
7	35.48330843070029	37.0	35.0	37.0	32.0	37.0
8	35.718896696392456	37.0	35.0	37.0	35.0	37.0
9	37.63536037789054	39.0	37.0	39.0	35.0	39.0
10	37.281038229263295	39.0	37.0	39.0	34.0	39.0
11	37.24290466353374	39.0	37.0	39.0	34.0	39.0
12	37.13536739571198	39.0	37.0	39.0	34.0	39.0
13	37.0386898875745	39.0	37.0	39.0	33.0	39.0
14	38.10266051990573	40.0	37.0	41.0	33.0	41.0
15	38.26488192834422	40.0	37.0	41.0	33.0	41.0
16	38.27534231019026	40.0	37.0	41.0	34.0	41.0
17	38.270496185495055	40.0	37.0	41.0	34.0	41.0
18	38.20125580724724	40.0	37.0	41.0	34.0	41.0
19	38.06265383383585	40.0	37.0	41.0	34.0	41.0
20	38.08707840437708	40.0	37.0	41.0	34.0	41.0
21	38.0336242964634	40.0	36.0	41.0	34.0	41.0
22	38.129190436878524	40.0	36.0	41.0	34.0	41.0
23	38.13065269567281	40.0	36.0	41.0	34.0	41.0
24	38.09115639661665	40.0	36.0	41.0	34.0	41.0
25	37.84676140300286	40.0	36.0	41.0	33.0	41.0
26	37.87864017587936	40.0	36.0	41.0	34.0	41.0
27	37.85759691930399	40.0	36.0	41.0	34.0	41.0
28	37.78255216474272	40.0	36.0	41.0	33.0	41.0
29	37.73707668182091	40.0	36.0	41.0	33.0	41.0
30	37.56039473331641	40.0	35.0	41.0	33.0	41.0
31	37.52161680398204	40.0	35.0	41.0	33.0	41.0
32	37.44059031362006	40.0	35.0	41.0	33.0	41.0
33	37.372218869263094	40.0	35.0	41.0	33.0	41.0
34	37.254170180893915	40.0	35.0	41.0	32.0	41.0
35	37.22288600888839	40.0	35.0	41.0	32.0	41.0
36	37.10380123488138	40.0	35.0	41.0	32.0	41.0
37	37.04460527306343	40.0	35.0	41.0	32.0	41.0
38	36.900771832761485	39.0	35.0	41.0	31.0	41.0
39	36.795473887962395	39.0	35.0	41.0	31.0	41.0
40	36.715514106459075	39.0	35.0	41.0	31.0	41.0
41	36.570487636512574	39.0	35.0	41.0	31.0	41.0
42	36.573213103165806	39.0	35.0	41.0	31.0	41.0
43	36.49665377514135	39.0	35.0	41.0	31.0	41.0
44	36.45100348466734	39.0	35.0	41.0	31.0	41.0
45	36.42484742618209	39.0	35.0	41.0	31.0	41.0
46	36.27594711880151	39.0	35.0	41.0	31.0	41.0
47	36.17104344797051	38.0	35.0	41.0	30.0	41.0
48	36.14229207152053	38.0	35.0	40.0	30.0	41.0
49	36.115704736008695	38.0	35.0	40.0	30.0	41.0
50	35.99199840759252	38.0	35.0	40.0	30.0	41.0
51	34.90763526212839	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	27.0
10	25.0
11	33.0
12	15.0
13	18.0
14	31.0
15	31.0
16	47.0
17	115.0
18	214.0
19	343.0
20	682.0
21	1155.0
22	1912.0
23	2709.0
24	3674.0
25	4931.0
26	6243.0
27	7246.0
28	7979.0
29	9423.0
30	11475.0
31	14976.0
32	20530.0
33	30755.0
34	53061.0
35	62248.0
36	63152.0
37	95361.0
38	149991.0
39	235155.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51069962575872	19.495380359542132	22.115834884697193	12.878085130001951
2	34.620699510921646	24.75083543974307	23.301336320798654	17.327128728536632
3	31.91105485512027	25.445216971899363	26.09379126957494	16.54993690340543
4	28.632073485522234	24.42533612174772	27.792359251211213	19.150231141518837
5	23.407879609911205	36.15032939101898	22.40701067602036	18.03478032304946
6	25.05757803498448	34.712951963650234	25.365724194513597	14.863745806851691
7	78.0564207324309	5.404998475218797	11.784836146629086	4.753744645721234
8	78.15339426503633	6.000875313728518	10.308031322451033	5.53769909878413
9	72.47750788228944	6.191632460103685	12.559603633445152	8.77125602416172
10	40.709999374775904	23.274030615565017	19.86821807305935	16.147751936599725
11	31.308160195171997	23.55333990881936	25.501614736914636	19.63688515909401
12	27.22136377961999	19.923212273786906	31.395181181010027	21.460242765583075
13	26.13845013327481	21.57712139172331	33.75827305450041	18.526155420501482
14	21.940134155226552	25.51348123498346	32.205292968525704	20.34109164126428
15	19.86387978344279	23.317668705237462	35.61837852597679	21.20007298534296
16	23.5838355328887	23.44871057100823	30.5622295746307	22.405224321472364
17	23.679277904453	22.915611335185186	29.113623632960284	24.29148712740153
18	24.71817067086545	22.1787400841373	30.702968793662016	22.400120451335237
19	24.057985068627914	25.1888750942621	27.415183248077433	23.33795658903255
20	23.5689067127376	27.01172231373745	27.908599893584306	21.510771079940643
21	23.725850719454293	26.658406903494747	29.758497624786433	19.857244752264524
22	22.247897524495386	22.57352443924417	29.97860202445009	25.199976011810353
23	21.435488995418	25.66034509817932	29.246579450032474	23.657586456370204
24	22.617034932163186	25.14370584354852	28.586904234808653	23.652354989479647
25	23.10394414324522	25.97142598303729	27.288352075169797	23.636277798547695
26	21.0125057578035	23.927581186624288	29.36754117228241	25.6923718832898
27	22.38417085715671	23.597105595245235	29.74561035269019	24.273113194907868
28	20.630608674792878	24.8405359574031	29.78810007158178	24.740755296222243
29	22.89340950008868	24.08605635438212	29.612016551850857	23.408517593678347
30	23.119383350410033	23.642912829725958	30.809512082774564	22.428191737089442
31	23.63232229919142	24.884046450322117	27.960659368983016	23.522971881503445
32	25.75604266325048	24.11948670378031	27.38239088244639	22.74207975052283
33	24.217736203920026	22.120300771067182	27.33390411614367	26.328058908869124
34	21.61910072360119	23.439268411254545	30.604974487029153	24.336656378115116
35	22.103713193121514	22.97647498657044	29.237902870799353	25.681908949508685
36	22.36362777985477	24.378508113239565	28.04589400027306	25.21197010663261
37	21.806412757633794	24.52983786280542	29.172190542783827	24.491558836776957
38	21.93643384937714	25.07863149930013	26.536807197477668	26.448127453845068
39	23.173229180356735	21.977137213720734	27.542397211245355	27.307236394677176
40	23.047035991216237	20.925739965472317	30.45173078616188	25.575493257149567
41	20.145868608519123	22.340787960991122	28.937795306736213	28.575548123753542
42	21.25060129970053	22.76836468172904	30.04112443362991	25.93990958494052
43	22.004060128694086	22.101416451559807	29.396505635310614	26.49801778443549
44	22.169935908150755	22.672539519904454	29.16134481874243	25.99617975320236
45	21.976244036446737	21.547901735188248	28.764263722073856	27.711590506291156
46	23.506129110050924	22.476678503392158	28.48150931647695	25.53568307007997
47	20.056168090859096	23.33272512214199	31.467400943450397	25.14370584354852
48	20.799929566592105	23.597871175765803	29.191074862291206	26.411124395350882
49	22.61894888346461	21.694893195137542	30.885559747817776	24.80059817358007
50	21.609020580080358	21.62701172231374	30.268119057978687	26.495848639627212
51	20.931737012883445	21.726026802974026	28.519915939258844	28.822320244883688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	780.0
2	1208.0
3	3927.0
4	6646.0
5	4287.5
6	1929.0
7	1868.5
8	1808.0
9	1831.0
10	1854.0
11	1824.5
12	1795.0
13	1783.0
14	1771.0
15	1768.0
16	1765.0
17	1716.0
18	1667.0
19	1682.0
20	1697.0
21	1978.0
22	2259.0
23	2369.0
24	2479.0
25	3011.5
26	4256.5
27	4969.0
28	6030.5
29	7092.0
30	8264.0
31	9436.0
32	10649.0
33	11862.0
34	13156.5
35	14451.0
36	16744.5
37	19038.0
38	20523.5
39	22009.0
40	24575.0
41	27141.0
42	31462.5
43	35784.0
44	41219.5
45	46655.0
46	62212.5
47	77770.0
48	79614.5
49	81459.0
50	81632.0
51	81805.0
52	71914.0
53	62023.0
54	54513.5
55	47004.0
56	44466.0
57	41928.0
58	39980.5
59	38033.0
60	33780.5
61	29528.0
62	26442.5
63	23357.0
64	21693.0
65	20029.0
66	17780.5
67	15532.0
68	13642.5
69	11753.0
70	10230.0
71	8707.0
72	7436.0
73	6165.0
74	4906.5
75	3176.5
76	2705.0
77	2174.5
78	1644.0
79	1179.0
80	714.0
81	529.0
82	344.0
83	278.0
84	212.0
85	153.5
86	95.0
87	63.0
88	31.0
89	21.5
90	12.0
91	7.5
92	3.0
93	3.0
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	783719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.563006741951185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79035366801236	24.81290477834571
2	10.649105269795777	7.361301944713987
3	4.137263317466534	4.289887798044694
4	2.2532172387868905	3.1151185046108774
5	1.4014230417290636	2.4218697019803677
6	0.9865794706165897	2.045949173663509
7	0.791501054897154	1.9149659407670265
8	0.6597575649303892	1.8242564131793866
9	0.5894276896193817	1.8335153889186662
>10	6.626244753321163	42.287475246529915
>50	0.07139365003427997	1.6822171996328417
>100	0.03625750629636665	2.3712013863973915
>500	0.0037378872470480748	0.8340675684091473
>1k	0.0033640985223432674	2.555171680296796
>5k	3.737887247048075E-4	0.6500972745097319
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	5032	0.6420668632507315	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4053	0.5171496416445179	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGC	3152	0.40218496680570454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG	3077	0.3926152102985892	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	2695	0.34387325048901457	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1695	0.21627649706080881	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT	1392	0.17761468077206244	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	1351	0.172383213881506	TruSeq Adapter, Index 22 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1299	0.1657481827032393	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1064	0.13576294564761093	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	836	0.10667088586598003	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	807	0.10297058001656205	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2759675342820579E-4	0.0	0.0	0.18016661584062654	0.0
2	1.2759675342820579E-4	0.0	0.0	0.8968775798468583	0.0
3	1.2759675342820579E-4	0.0	0.0	1.296638208337427	0.0
4	1.2759675342820579E-4	0.0	0.0	1.9145892851902275	0.0
5	1.2759675342820579E-4	0.0	0.0	3.7283771351721726	0.0
6	1.2759675342820579E-4	0.0	0.0	4.9566234836720815	0.0
7	1.2759675342820579E-4	0.0	0.0	6.044258209894108	0.0
8	1.2759675342820579E-4	0.0	0.0	7.669968445322877	0.0
9	1.2759675342820579E-4	0.0	0.0	8.440014852262099	0.0
10	1.2759675342820579E-4	0.0	0.0	10.163974587830587	0.0
11	1.2759675342820579E-4	0.0	0.0	11.695901209489625	0.0
12	1.2759675342820579E-4	0.0	0.0	13.35108629496031	0.0
13	1.2759675342820579E-4	0.0	0.0	13.952960180881158	0.0
14	1.2759675342820579E-4	0.0	0.0	14.28343577226021	0.0
15	1.2759675342820579E-4	0.0	0.0	14.718413104696964	0.0
16	1.2759675342820579E-4	0.0	0.0	15.418026103743816	0.0
17	1.2759675342820579E-4	0.0	0.0	16.176716399627928	0.0
18	1.2759675342820579E-4	0.0	0.0	17.050243773597426	0.0
19	2.5519350685641157E-4	0.0	0.0	17.615497391284375	0.0
20	2.5519350685641157E-4	0.0	0.0	18.169777688176502	0.0
21	2.5519350685641157E-4	0.0	0.0	18.752512061083117	0.0
22	2.5519350685641157E-4	0.0	0.0	19.41091130877266	0.0
23	2.5519350685641157E-4	0.0	0.0	20.043918802529987	0.0
24	3.827902602846173E-4	0.0	0.0	20.572424555229617	0.0
25	3.827902602846173E-4	0.0	0.0	21.033431625365726	0.0
26	3.827902602846173E-4	0.0	0.0	21.48104103639187	0.0
27	3.827902602846173E-4	0.0	0.0	21.99653192024182	0.0
28	3.827902602846173E-4	0.0	0.0	22.457666587131357	0.0
29	3.827902602846173E-4	0.0	0.0	22.968563987857895	0.0
30	3.827902602846173E-4	0.0	0.0	23.670346131713025	0.0
31	3.827902602846173E-4	0.0	0.0	24.17996756490528	0.0
32	3.827902602846173E-4	0.0	0.0	24.718425864372307	0.0
33	3.827902602846173E-4	0.0	0.0	25.23672387679768	0.0
34	5.103870137128231E-4	0.0	0.0	25.788197045114384	0.0
35	5.103870137128231E-4	0.0	0.0	26.344901680321644	0.0
36	5.103870137128231E-4	0.0	0.0	26.863454886253873	0.0
37	5.103870137128231E-4	0.0	0.0	27.38647397855609	0.0
38	5.103870137128231E-4	0.0	0.0	27.93692637284537	0.0
39	6.379837671410288E-4	0.0	0.0	28.74346545126506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	20	7.0319104E-4	45.000004	1
CGAGCAT	20	7.0319104E-4	45.000004	14
CGTAAAA	20	7.0319104E-4	45.000004	14
ATCGGGT	20	7.0319104E-4	45.000004	5
CCACGCG	20	7.0319104E-4	45.000004	12
CGAAGTT	20	7.0319104E-4	45.000004	38
CGCAAAT	25	3.889666E-5	45.0	10
CGTCAAT	25	3.889666E-5	45.0	38
TAACGCC	60	3.6379788E-12	41.249996	12
ATAACGC	60	3.6379788E-12	41.249996	11
GCGATAT	50	1.0804797E-9	40.5	9
GTTCGAT	45	1.9270374E-8	40.0	39
TCGATTA	40	3.4563345E-7	39.375004	41
TTGAGCG	40	3.4563345E-7	39.375004	1
CGTTTTT	1790	0.0	38.840782	1
AACGTTG	35	6.2457457E-6	38.571426	1
ATATGCG	35	6.2457457E-6	38.571426	1
TAACGGG	140	0.0	38.571426	3
ATACCGG	35	6.2457457E-6	38.571426	2
TGGGCGA	370	0.0	38.31081	6
>>END_MODULE