SRR2936398.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246720
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	4077	1.6524805447470818	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	3883	1.573848897535668	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	3527	1.4295557717250325	No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	2106	0.8535992217898832	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1039	0.42112516212710766	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	897	0.3635700389105058	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	759	0.30763618677042803	TruSeq Adapter, Index 13 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	684	0.2772373540856031	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	663	0.26872568093385213	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	661	0.26791504539559013	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	632	0.25616083009079116	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.20468547341115437	No Hit
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	502	0.20346952010376138	TruSeq Adapter, Index 19 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	443	0.17955577172503243	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	413	0.16739623865110245	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	387	0.1568579766536965	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	362	0.14672503242542154	No Hit
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	319	0.12929636835278857	TruSeq Adapter, Index 13 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATAC	170	0.0	45.000004	3
GCCGATG	35	1.206754E-7	45.000004	9
CGGGCCC	35	1.206754E-7	45.000004	6
CTCGAAT	35	1.206754E-7	45.000004	43
ACAGTCG	20	7.022507E-4	45.0	1
CTGTATG	20	7.022507E-4	45.0	22
CACGGGC	20	7.022507E-4	45.0	4
ATTACGG	40	6.7775545E-9	45.0	2
CGATTGT	20	7.022507E-4	45.0	10
CGCGAGG	20	7.022507E-4	45.0	2
CGTTATT	50	2.1827873E-11	45.0	1
GTTACTT	20	7.022507E-4	45.0	12
ACTACGG	20	7.022507E-4	45.0	2
GAGCATG	45	3.8198777E-10	45.0	1
GGCGAAC	25	3.8818733E-5	45.0	32
ATGTTAC	20	7.022507E-4	45.0	10
GTTGGCC	20	7.022507E-4	45.0	18
TCGAAGG	20	7.022507E-4	45.0	2
TGGTACG	20	7.022507E-4	45.0	1
AGAGACG	20	7.022507E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content