##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246720
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79721141374838	33.0	31.0	34.0	30.0	34.0
2	31.891885538262	33.0	31.0	34.0	30.0	34.0
3	31.86801637483787	33.0	31.0	34.0	30.0	34.0
4	35.54969195849546	37.0	35.0	37.0	33.0	37.0
5	31.816755836575876	37.0	35.0	37.0	0.0	37.0
6	33.63066228923476	37.0	35.0	37.0	17.0	37.0
7	35.286616407263296	37.0	35.0	37.0	32.0	37.0
8	35.47807230869001	37.0	35.0	37.0	33.0	37.0
9	37.40407344357977	39.0	37.0	39.0	34.0	39.0
10	37.04896238651102	39.0	37.0	39.0	33.0	39.0
11	37.03578955901427	39.0	37.0	39.0	33.0	39.0
12	36.864992704280155	39.0	35.0	39.0	33.0	39.0
13	36.6102140077821	39.0	35.0	39.0	32.0	39.0
14	37.729069390402074	40.0	36.0	41.0	33.0	41.0
15	37.85323443579767	40.0	36.0	41.0	33.0	41.0
16	37.940847924773024	40.0	36.0	41.0	33.0	41.0
17	37.89207603761349	40.0	36.0	41.0	33.0	41.0
18	37.858029345006486	40.0	36.0	41.0	33.0	41.0
19	37.741776102464335	39.0	36.0	41.0	33.0	41.0
20	37.70585278858625	40.0	36.0	41.0	33.0	41.0
21	37.74371757457847	39.0	35.0	41.0	33.0	41.0
22	37.822920719844355	40.0	36.0	41.0	33.0	41.0
23	37.811352950713356	40.0	36.0	41.0	34.0	41.0
24	37.764283398184176	40.0	35.0	41.0	33.0	41.0
25	37.56838521400778	39.0	35.0	41.0	33.0	41.0
26	37.58116083009079	39.0	35.0	41.0	33.0	41.0
27	37.50940337224384	39.0	35.0	41.0	33.0	41.0
28	37.429738164721144	39.0	35.0	41.0	33.0	41.0
29	37.393851329442285	39.0	35.0	41.0	33.0	41.0
30	37.25188067444877	39.0	35.0	41.0	32.0	41.0
31	37.25057960440986	39.0	35.0	41.0	33.0	41.0
32	37.116816634241246	39.0	35.0	41.0	32.0	41.0
33	37.04268806744488	39.0	35.0	41.0	32.0	41.0
34	36.97069552529183	39.0	35.0	41.0	32.0	41.0
35	36.96235813878081	39.0	35.0	41.0	32.0	41.0
36	36.88654345006485	39.0	35.0	41.0	32.0	41.0
37	36.78622730220493	39.0	35.0	41.0	31.0	41.0
38	36.64647778858625	39.0	35.0	41.0	31.0	41.0
39	36.64289477950713	39.0	35.0	41.0	31.0	41.0
40	36.54459306095979	39.0	35.0	41.0	31.0	41.0
41	36.52280317769131	39.0	35.0	41.0	30.0	41.0
42	36.52699011024643	39.0	35.0	41.0	31.0	41.0
43	36.47732247081712	39.0	35.0	41.0	31.0	41.0
44	36.47698605706874	39.0	35.0	41.0	31.0	41.0
45	36.49115596627756	39.0	35.0	41.0	31.0	41.0
46	36.36566553177691	39.0	35.0	41.0	31.0	41.0
47	36.21214737354086	38.0	35.0	40.0	30.0	41.0
48	36.228741083009076	38.0	35.0	40.0	30.0	41.0
49	36.26804069390402	39.0	35.0	40.0	31.0	41.0
50	36.11119082360571	38.0	35.0	40.0	30.0	41.0
51	35.154839494163426	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	10.0
11	8.0
12	4.0
13	6.0
14	7.0
15	8.0
16	11.0
17	27.0
18	55.0
19	131.0
20	253.0
21	528.0
22	839.0
23	1192.0
24	1398.0
25	1779.0
26	2050.0
27	2198.0
28	2552.0
29	2968.0
30	3894.0
31	5343.0
32	7046.0
33	10667.0
34	17311.0
35	24387.0
36	18714.0
37	27909.0
38	45227.0
39	70145.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.41569390402075	18.15296692607004	21.970654993514916	12.460684176394293
2	29.935959792477302	29.377026588845656	23.353599221789885	17.333414396887157
3	28.712710765239947	29.930285343709468	24.94690337224384	16.410100518806743
4	26.976329442282747	24.010619325551232	32.02294098573281	16.990110246433204
5	25.498540856031127	34.70817120622568	22.09914072632944	17.69414721141375
6	22.758592736705577	37.49635214007782	25.03688391699092	14.708171206225682
7	73.16715304798961	5.096870946822309	16.198929961089494	5.537046044098573
8	71.00599870298313	10.282911802853437	11.311608300907912	7.399481193255513
9	66.58033398184176	6.040045395590143	12.88221465629053	14.497405966277563
10	41.190823605706875	21.99700064850843	21.009241245136188	15.802934500648508
11	32.396643968871594	21.718142023346303	24.777480544747082	21.107733463035018
12	27.133187418936444	17.895184824902724	32.23411154345007	22.737516212710766
13	23.228761348897535	25.250081063553825	34.49578469520104	17.0253728923476
14	17.919098573281453	31.873378728923473	28.69811932555123	21.509403372243842
15	14.049124513618677	23.536397535667962	42.51094357976653	19.903534370946822
16	15.582036316472115	23.861867704280158	33.94576848249027	26.610327496757456
17	16.803258754863815	24.156939040207522	30.772130350194555	28.26767185473411
18	18.69649805447471	23.41642347600519	33.418855382619974	24.468223086900128
19	20.4628728923476	26.143401426718547	27.83276588845655	25.5609597924773
20	22.75575551232166	25.596222438391695	31.29134241245136	20.356679636835278
21	20.16536964980545	30.61243514915694	29.149643320363168	20.07255188067445
22	17.440823605706875	23.775940337224384	29.02075226977951	29.762483787289234
23	17.984354734111545	28.834306095979244	28.21862840466926	24.962710765239947
24	20.74578469520104	23.683122568093385	29.289477950713362	26.28161478599222
25	16.93985084306096	30.175907911802852	27.44285019455253	25.441391050583658
26	17.266536964980546	25.42679961089494	29.157344357976655	28.14931906614786
27	20.906290531776914	26.661397535667962	28.45776588845655	23.974546044098574
28	17.351248378728922	24.477950713359274	33.702172503242544	24.46862840466926
29	19.006971465629054	22.262483787289234	30.995460440985735	27.73508430609598
30	19.90393968871595	27.737516212710766	30.569876783398186	21.788667315175097
31	24.790450713359274	24.482409208819714	22.775210765239947	27.951929312581065
32	22.469601167315176	32.078874837872895	22.17169260700389	23.27983138780804
33	20.701199740596625	27.03347924773022	24.698038261997407	27.567282749675748
34	20.216439688715955	24.514834630350194	25.271968223086898	29.99675745784695
35	17.78250648508431	27.60051880674449	25.929798962386513	28.687175745784693
36	22.009565499351492	25.00445849546044	25.107814526588847	27.87816147859922
37	18.419260700389106	27.435959792477306	30.90021076523995	23.244568741893644
38	19.87475680933852	30.780236705577174	22.449740596627755	26.89526588845655
39	19.766131647211413	27.542963683527887	25.563391699092087	27.12751297016861
40	19.965547989623865	27.46635862516213	27.255998702983135	25.312094682230867
41	16.763537613488975	24.672503242542156	24.385943579766536	34.17801556420233
42	20.044990272373543	24.30650129701686	28.36089494163424	27.287613488975353
43	21.93214980544747	23.407506485084305	25.878323605706875	28.782020103761347
44	20.65337224383917	23.297260051880674	28.564364461738002	27.485003242542156
45	19.577658884565498	24.22786964980545	26.244325551232166	29.950145914396884
46	24.925826848249027	25.89250972762646	25.64283398184176	23.53882944228275
47	17.099546044098574	23.699740596627755	32.5863326848249	26.614380674448768
48	19.771806095979247	23.994406614785994	27.138456549935146	29.095330739299612
49	20.021887159533076	19.581306744487676	34.11113813229572	26.28566796368353
50	20.727545395590145	21.17217898832685	30.221708819714653	27.878566796368354
51	18.650697146562905	21.766780155642024	26.15637159533074	33.42615110246433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	197.0
2	270.0
3	622.0
4	974.0
5	689.0
6	404.0
7	417.0
8	430.0
9	465.0
10	500.0
11	480.5
12	461.0
13	459.5
14	458.0
15	451.0
16	444.0
17	483.0
18	522.0
19	595.0
20	668.0
21	630.0
22	592.0
23	626.0
24	660.0
25	860.5
26	1111.0
27	1161.0
28	1361.5
29	1562.0
30	1821.0
31	2080.0
32	2396.5
33	2713.0
34	3233.0
35	3753.0
36	4324.5
37	4896.0
38	5689.5
39	6483.0
40	7996.0
41	9509.0
42	11522.5
43	13536.0
44	16878.0
45	20220.0
46	29879.0
47	39538.0
48	35944.0
49	32350.0
50	31651.5
51	30953.0
52	25585.5
53	20218.0
54	16703.0
55	13188.0
56	11446.5
57	9705.0
58	8884.5
59	8064.0
60	6923.0
61	5782.0
62	5126.5
63	4471.0
64	3828.0
65	3185.0
66	2608.5
67	2032.0
68	1697.0
69	1362.0
70	1092.0
71	822.0
72	763.5
73	705.0
74	540.0
75	276.5
76	178.0
77	150.5
78	123.0
79	104.5
80	86.0
81	65.5
82	45.0
83	45.5
84	46.0
85	26.0
86	6.0
87	3.5
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	246720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.925178339818416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43885261144182	27.85181582360571
2	11.417242890273489	8.660019455252918
3	4.425611046393571	5.035262645914397
4	2.205858778014086	3.3463035019455254
5	1.2482766728296764	2.3670557717250325
6	0.8603276726266177	1.9576848249027237
7	0.6102448460494394	1.620055123216602
8	0.5268839038570468	1.5985732814526588
9	0.41252979084953356	1.4080739299610896
>10	4.736611484572882	33.88780804150454
>50	0.07267364191131678	1.888780804150454
>100	0.030993170815120392	2.2985570687418937
>500	0.008549840224860798	2.1494001297016863
>1k	0.005343650140537999	5.930609597924772
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	4077	1.6524805447470818	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	3883	1.573848897535668	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	3527	1.4295557717250325	No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	2106	0.8535992217898832	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1039	0.42112516212710766	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	897	0.3635700389105058	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	759	0.30763618677042803	TruSeq Adapter, Index 13 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	684	0.2772373540856031	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	663	0.26872568093385213	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	661	0.26791504539559013	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	632	0.25616083009079116	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.20468547341115437	No Hit
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	502	0.20346952010376138	TruSeq Adapter, Index 19 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	443	0.17955577172503243	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	413	0.16739623865110245	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	387	0.1568579766536965	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	362	0.14672503242542154	No Hit
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	319	0.12929636835278857	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.053177691309987E-4	0.0	0.0	0.323443579766537	0.0
2	4.053177691309987E-4	0.0	0.0	2.0363164721141374	0.0
3	4.053177691309987E-4	0.0	0.0	2.6272697795071336	0.0
4	4.053177691309987E-4	0.0	0.0	3.6340791180285343	0.0
5	4.053177691309987E-4	0.0	0.0	7.059014267185473	0.0
6	4.053177691309987E-4	0.0	0.0	8.390483138780803	0.0
7	4.053177691309987E-4	0.0	0.0	10.055528534370946	0.0
8	4.053177691309987E-4	0.0	0.0	12.506485084306096	0.0
9	4.053177691309987E-4	0.0	0.0	13.355220492866406	0.0
10	4.053177691309987E-4	0.0	0.0	16.6605869001297	0.0
11	4.053177691309987E-4	0.0	0.0	18.812418936446175	0.0
12	4.053177691309987E-4	0.0	0.0	22.476086251621272	0.0
13	4.053177691309987E-4	0.0	0.0	23.398994811932557	0.0
14	4.053177691309987E-4	0.0	0.0	23.838764591439688	0.0
15	4.053177691309987E-4	0.0	0.0	24.61413748378729	0.0
16	4.053177691309987E-4	0.0	0.0	25.732409208819714	0.0
17	4.053177691309987E-4	0.0	0.0	27.055771725032425	0.0
18	4.053177691309987E-4	0.0	0.0	28.642185473411153	0.0
19	8.106355382619975E-4	0.0	0.0	29.783154993514916	0.0
20	8.106355382619975E-4	0.0	0.0	30.605544747081712	0.0
21	8.106355382619975E-4	0.0	0.0	31.52561608300908	0.0
22	8.106355382619975E-4	0.0	0.0	32.458657587548636	0.0
23	8.106355382619975E-4	0.0	0.0	33.448038261997404	0.0
24	8.106355382619975E-4	0.0	0.0	34.170719844357976	0.0
25	8.106355382619975E-4	0.0	0.0	34.757619974059665	0.0
26	8.106355382619975E-4	0.0	0.0	35.322632944228275	0.0
27	0.0012159533073929961	0.0	0.0	35.920881971465626	0.0
28	0.0012159533073929961	0.0	0.0	36.49359597924773	0.0
29	0.001621271076523995	0.0	0.0	37.083333333333336	0.0
30	0.0020265888456549937	0.0	0.0	37.773589494163424	0.0
31	0.0020265888456549937	0.0	0.0	38.37264915693904	0.0
32	0.0020265888456549937	0.0	0.0	38.929555771725035	0.0
33	0.0020265888456549937	0.0	0.0	39.48078793774319	0.0
34	0.0020265888456549937	0.0	0.0	40.029588197146566	0.0
35	0.0020265888456549937	0.0	0.0	40.70322632944228	0.0
36	0.0020265888456549937	0.0	0.0	41.23013942931258	0.0
37	0.0020265888456549937	0.0	0.0	41.7534046692607	0.0
38	0.0020265888456549937	0.0	0.0	42.24140726329442	0.0
39	0.0020265888456549937	0.0	0.0	42.76386186770428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATAC	170	0.0	45.000004	3
GCCGATG	35	1.206754E-7	45.000004	9
CGGGCCC	35	1.206754E-7	45.000004	6
CTCGAAT	35	1.206754E-7	45.000004	43
ACAGTCG	20	7.022507E-4	45.0	1
CTGTATG	20	7.022507E-4	45.0	22
CACGGGC	20	7.022507E-4	45.0	4
ATTACGG	40	6.7775545E-9	45.0	2
CGATTGT	20	7.022507E-4	45.0	10
CGCGAGG	20	7.022507E-4	45.0	2
CGTTATT	50	2.1827873E-11	45.0	1
GTTACTT	20	7.022507E-4	45.0	12
ACTACGG	20	7.022507E-4	45.0	2
GAGCATG	45	3.8198777E-10	45.0	1
GGCGAAC	25	3.8818733E-5	45.0	32
ATGTTAC	20	7.022507E-4	45.0	10
GTTGGCC	20	7.022507E-4	45.0	18
TCGAAGG	20	7.022507E-4	45.0	2
TGGTACG	20	7.022507E-4	45.0	1
AGAGACG	20	7.022507E-4	45.0	1
>>END_MODULE