##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310748
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.957663444334315	33.0	31.0	34.0	30.0	34.0
2	32.08436096129339	33.0	31.0	34.0	30.0	34.0
3	31.614121410274564	33.0	31.0	34.0	28.0	34.0
4	35.55602610475369	37.0	35.0	37.0	33.0	37.0
5	35.756439301298805	37.0	35.0	37.0	35.0	37.0
6	35.77733726363484	37.0	35.0	37.0	35.0	37.0
7	26.986896134488397	35.0	11.0	37.0	0.0	37.0
8	31.285842547659197	37.0	22.0	37.0	17.0	37.0
9	36.079601477724715	39.0	32.0	39.0	32.0	39.0
10	36.77258421614942	38.0	35.0	39.0	33.0	39.0
11	37.21347200947392	39.0	37.0	39.0	34.0	39.0
12	37.268297784700145	39.0	37.0	39.0	34.0	39.0
13	37.255132776397595	39.0	37.0	39.0	34.0	39.0
14	38.4028505412746	40.0	38.0	41.0	34.0	41.0
15	38.486873608196994	40.0	38.0	41.0	34.0	41.0
16	38.43201565255448	40.0	38.0	41.0	34.0	41.0
17	38.34891294553786	40.0	38.0	41.0	34.0	41.0
18	38.18379844761672	40.0	37.0	41.0	34.0	41.0
19	38.008476321649695	40.0	37.0	41.0	34.0	41.0
20	37.97980678878062	40.0	36.0	41.0	34.0	41.0
21	37.912523974410135	40.0	36.0	41.0	33.0	41.0
22	37.926113764207656	40.0	36.0	41.0	33.0	41.0
23	37.85429672918249	40.0	36.0	41.0	33.0	41.0
24	37.80874534992984	40.0	36.0	41.0	33.0	41.0
25	37.71833447037471	40.0	35.0	41.0	33.0	41.0
26	37.61182051051012	40.0	35.0	41.0	33.0	41.0
27	37.50148029914915	39.0	35.0	41.0	33.0	41.0
28	37.45329977988595	39.0	35.0	41.0	33.0	41.0
29	37.40993345089912	39.0	35.0	41.0	33.0	41.0
30	37.28599057757411	39.0	35.0	41.0	33.0	41.0
31	37.06952900742724	39.0	35.0	41.0	32.0	41.0
32	36.848250028962376	39.0	35.0	41.0	31.0	41.0
33	36.64288426635087	39.0	35.0	41.0	30.0	41.0
34	36.319976315213616	39.0	35.0	41.0	30.0	41.0
35	36.08505284024354	39.0	35.0	41.0	29.0	41.0
36	35.83773990500341	39.0	35.0	41.0	27.0	41.0
37	35.725999845534005	39.0	35.0	41.0	26.0	41.0
38	35.52477570249849	39.0	35.0	41.0	25.0	41.0
39	35.45958139714495	39.0	35.0	41.0	24.0	41.0
40	35.316877341125284	39.0	35.0	40.0	24.0	41.0
41	35.191611852690926	39.0	35.0	40.0	23.0	41.0
42	35.09450744654833	39.0	34.0	40.0	23.0	41.0
43	35.04785227901708	38.0	34.0	40.0	23.0	41.0
44	34.805701082549206	38.0	34.0	40.0	22.0	41.0
45	34.72264342811538	38.0	34.0	40.0	22.0	41.0
46	34.598336916086346	38.0	34.0	40.0	22.0	41.0
47	34.45806569953789	38.0	34.0	40.0	21.0	41.0
48	34.36245446471096	38.0	33.0	40.0	20.0	41.0
49	34.22367641947816	38.0	33.0	40.0	20.0	41.0
50	34.0192277987308	38.0	33.0	40.0	20.0	41.0
51	32.0236847863864	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	16.0
10	28.0
11	14.0
12	7.0
13	8.0
14	14.0
15	16.0
16	30.0
17	71.0
18	101.0
19	215.0
20	415.0
21	607.0
22	1074.0
23	1667.0
24	2627.0
25	4009.0
26	5365.0
27	5916.0
28	6045.0
29	6052.0
30	6506.0
31	7697.0
32	9735.0
33	12903.0
34	18847.0
35	23562.0
36	27686.0
37	44866.0
38	58988.0
39	65636.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.92928031717018	21.189516907590715	25.163154710569337	16.718048064669762
2	35.06249436842715	23.54673240053033	25.477557377682235	15.91321585336028
3	29.877585696448573	23.356867944443728	31.243966171946404	15.521580187161302
4	26.1736197819455	24.936282775753988	31.463758415178862	17.426339027121653
5	24.221877534207785	28.84041087955514	29.514912404906866	17.42279918133021
6	25.353662774981657	32.88902905247982	28.115707904797453	13.641600267741063
7	59.97078018201244	26.841685224040056	9.78091572592583	3.4066188680216767
8	82.20068994812517	3.876452945795307	10.967729478548534	2.95512762753099
9	75.51713928971385	7.427883687103376	12.655592312742158	4.399384710440614
10	31.94035038037252	37.489219560544235	18.43648229433496	12.133947764748285
11	29.132287255267936	24.06966416517564	29.65586262823896	17.142185951317465
12	27.151582632872937	22.09282119273495	31.58025152213369	19.17534465225842
13	25.05020144940595	21.752030584267636	34.11317208799413	19.084595878332284
14	20.615418281050886	24.694607849447138	34.084853321662564	20.605120547839405
15	20.196751065171778	24.94497148815117	35.657510265552794	19.200767181124256
16	24.547221542857876	25.370396591450305	32.112837411664756	17.969544454027055
17	24.42525776513445	24.339336053651188	31.426750936450116	19.808655244764246
18	24.342232291116918	23.9695830705266	32.65346840526729	19.034716233089192
19	23.022835223396452	26.080940183042205	30.858766589004595	20.037458004556747
20	23.613024058079215	26.87450924865164	30.97686871677372	18.535597976495424
21	23.976019153783774	25.884961447861286	32.329089809105774	17.809929589249165
22	23.773604335345684	22.842946696358464	32.61742633902712	20.766022629268733
23	20.682675351088342	25.22140126404675	33.32089023903613	20.775033145828775
24	21.390644509377374	24.778276931790387	33.099488974989384	20.731589583842858
25	22.102797121783567	26.25213999768301	30.581693204783296	21.063369675750128
26	21.616872835867003	26.090916112090827	30.836883905930208	21.455327146111962
27	21.397080592634545	25.229124563955356	31.99312626308134	21.380668580328756
28	20.083797804008395	26.01239589635331	32.312034188474264	21.59177211116403
29	22.279145803030108	25.165729143872205	31.718627312162912	20.836497740934774
30	23.398380681452497	24.36121873672558	31.803583611157592	20.43681697066433
31	22.970381144850492	24.668863516418448	31.586365801228006	20.77438953750306
32	23.801923101677243	25.413196545110505	31.068582903188435	19.716297450023816
33	23.206907204551598	24.87127833485654	30.911220667550555	21.010593793041306
34	20.983240439198322	25.38648679959324	32.788947957830786	20.841324803377656
35	21.18179360768211	26.647959117999147	31.08628213214566	21.083965142173078
36	21.941895040354243	27.381672609316876	29.83671656776552	20.839715782563363
37	21.32757089345708	27.618842277343695	29.892710492102925	21.160876337096298
38	21.270289752468237	27.71055646375842	29.824488009576893	21.194665774196455
39	21.046957663444335	26.2646903600345	29.92521271255165	22.763139263969517
40	22.715834052029297	26.02977332114768	29.487880855226745	21.766511771596278
41	20.83102707016618	26.242807676960112	29.412578681117818	23.513586571755894
42	21.499736120586455	26.432672133046715	29.8508759509313	22.21671579543553
43	21.550581178318122	26.016901154633338	30.186517692792876	22.245999974255668
44	21.833768841633734	25.927117793195777	30.217410892427303	22.021702472743186
45	21.23971835699667	25.495578410802324	29.51587781739545	23.748825414805566
46	21.631032219032786	26.501216419735606	29.60823561213588	22.25951574909573
47	20.59964987707081	26.223177623025734	30.952089796233608	22.225082703669855
48	21.053393746701506	26.062597345759265	30.39504679032528	22.488962117213948
49	21.68284268925303	25.113918673651963	30.83881473090736	22.36442390618765
50	20.540759715267676	25.698315033403276	30.945010104650716	22.815915146678336
51	20.349929846692497	24.853900910062173	30.377991169693768	24.418178073551562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	238.0
2	395.0
3	3856.5
4	7318.0
5	5027.0
6	2736.0
7	2346.5
8	1957.0
9	1849.0
10	1741.0
11	1743.0
12	1745.0
13	1660.0
14	1575.0
15	1500.5
16	1426.0
17	1368.0
18	1310.0
19	1282.5
20	1255.0
21	1300.0
22	1345.0
23	1518.5
24	1692.0
25	2002.0
26	2695.0
27	3078.0
28	3701.5
29	4325.0
30	5049.0
31	5773.0
32	6657.0
33	7541.0
34	8683.0
35	9825.0
36	10834.5
37	11844.0
38	12305.0
39	12766.0
40	13836.0
41	14906.0
42	15914.0
43	16922.0
44	18529.5
45	20137.0
46	22689.5
47	25242.0
48	25721.5
49	26201.0
50	25564.0
51	24927.0
52	22735.5
53	20544.0
54	18662.0
55	16780.0
56	15710.5
57	14641.0
58	13686.0
59	12731.0
60	11769.0
61	10807.0
62	9492.0
63	8177.0
64	6826.5
65	5476.0
66	4533.0
67	3590.0
68	3170.5
69	2751.0
70	2311.5
71	1872.0
72	1521.0
73	1170.0
74	982.0
75	617.0
76	440.0
77	365.0
78	290.0
79	213.0
80	136.0
81	107.5
82	79.0
83	57.5
84	36.0
85	34.0
86	32.0
87	19.5
88	7.0
89	8.0
90	9.0
91	6.0
92	3.0
93	4.5
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	310748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.52649939895553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53725560707169	35.6400060303468
2	11.130140204656701	11.247340447217551
3	5.732256745674515	8.688926010449464
4	3.7154629806285944	7.509173522302888
5	2.4921243666391533	6.295916015655781
6	1.7383397909630802	5.269933464196593
7	1.2777337590484845	4.5191589806011905
8	0.899965053843218	3.637766700167227
9	0.6650556824524113	3.0242641985733347
>10	1.7926867381352372	11.571328122567518
>50	0.008856899747230003	0.31229675181475747
>100	0.008856899747230003	0.8985324148938416
>500	6.326356962307143E-4	0.2122467177123838
>1k	6.326356962307143E-4	1.1731106235006754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3670	1.1810212776912483	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	664	0.2136779641381441	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	338	0.10876980704622395	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	334	0.10748259039478934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2180416285865074E-4	0.0	0.0	0.08334727818039055	0.0
2	3.2180416285865074E-4	0.0	0.0	0.29799065480711057	0.0
3	3.2180416285865074E-4	0.0	0.0	0.4566401070964254	0.0
4	3.2180416285865074E-4	0.0	0.0	0.640390284088715	0.0
5	3.2180416285865074E-4	0.0	0.0	1.2151325189542652	0.0
6	3.2180416285865074E-4	0.0	0.0	1.758016141696809	0.0
7	3.2180416285865074E-4	0.0	0.0	2.2304246527733085	0.0
8	3.2180416285865074E-4	0.0	0.0	3.0410493390142497	0.0
9	3.2180416285865074E-4	0.0	0.0	3.55368337044808	0.0
10	3.2180416285865074E-4	0.0	0.0	4.385547131437693	0.0
11	3.2180416285865074E-4	0.0	0.0	5.052325356880816	0.0
12	3.2180416285865074E-4	0.0	0.0	5.635434499980692	0.0
13	3.2180416285865074E-4	0.0	0.0	5.933746958950661	0.0
14	3.2180416285865074E-4	0.0	0.0	6.112026465174353	0.0
15	3.2180416285865074E-4	0.0	0.0	6.292236796375198	0.0
16	3.2180416285865074E-4	0.0	0.0	6.595054513625188	0.0
17	3.2180416285865074E-4	0.0	0.0	6.936810534581076	0.0
18	3.2180416285865074E-4	0.0	0.0	7.340674758968682	0.0
19	3.2180416285865074E-4	0.0	0.0	7.580740664461236	0.0
20	3.2180416285865074E-4	0.0	0.0	7.846550902982481	0.0
21	3.2180416285865074E-4	0.0	0.0	8.181549036518337	0.0
22	3.2180416285865074E-4	0.0	0.0	8.534568203174276	0.0
23	3.2180416285865074E-4	0.0	0.0	8.87728963661874	0.0
24	3.2180416285865074E-4	0.0	0.0	9.167878795680101	0.0
25	3.2180416285865074E-4	0.0	0.0	9.410519134475523	0.0
26	3.2180416285865074E-4	0.0	0.0	9.682121847928224	0.0
27	3.2180416285865074E-4	0.0	0.0	9.955011778032361	0.0
28	3.2180416285865074E-4	0.0	0.0	10.232406966416518	0.0
29	3.2180416285865074E-4	0.0	0.0	10.518169063035	0.0
30	3.2180416285865074E-4	0.0	0.0	10.884060396205285	0.0
31	3.2180416285865074E-4	0.0	0.0	11.182051051012396	0.0
32	3.2180416285865074E-4	0.0	0.0	11.480685314145223	0.0
33	3.2180416285865074E-4	0.0	0.0	11.77706694813804	0.0
34	3.2180416285865074E-4	0.0	0.0	12.104341781765289	0.0
35	3.2180416285865074E-4	0.0	0.0	12.427111357112516	0.0
36	3.2180416285865074E-4	0.0	0.0	12.725745620245343	0.0
37	3.2180416285865074E-4	0.0	0.0	13.051411433058298	0.0
38	3.2180416285865074E-4	0.0	0.0	13.404752403877097	0.0
39	3.2180416285865074E-4	0.0	0.0	13.889389473142225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.0	25
CGGTTTA	25	3.8842147E-5	45.0	1
GCTTACG	20	7.025334E-4	45.0	1
GCGATCG	25	3.8842147E-5	45.0	9
TATAGCG	25	3.8842147E-5	45.0	1
CGGTCTA	40	6.7866495E-9	45.0	31
CGCACGG	20	7.025334E-4	45.0	2
CGTTTTA	230	0.0	44.02174	1
CGTTTTT	1655	0.0	43.640484	1
TAGGGCG	75	0.0	42.0	5
TTCCGCC	65	0.0	41.538464	34
CGCCCAA	65	0.0	41.538464	37
ATTTCCG	60	3.6379788E-12	41.250004	32
TCTCACG	55	6.002665E-11	40.90909	23
CGACGGT	50	1.0768417E-9	40.5	28
GACGGTC	50	1.0768417E-9	40.5	29
GGCGATC	90	0.0	40.0	8
AGGCGAT	125	0.0	39.600002	7
CGTTTCT	40	3.447367E-7	39.375	1
TCCGCCC	70	0.0	38.571426	35
>>END_MODULE