##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129434
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.004372885022484	33.0	31.0	34.0	30.0	34.0
2	32.12637328677164	33.0	31.0	34.0	30.0	34.0
3	32.033005238190896	33.0	31.0	34.0	30.0	34.0
4	35.75897368543041	37.0	35.0	37.0	35.0	37.0
5	35.83131171098784	37.0	35.0	37.0	35.0	37.0
6	35.80566157269342	37.0	35.0	37.0	35.0	37.0
7	26.8875565925491	35.0	11.0	37.0	0.0	37.0
8	31.180702133906085	36.0	19.0	37.0	17.0	37.0
9	35.970262836658065	39.0	32.0	39.0	32.0	39.0
10	36.676506945624794	38.0	35.0	39.0	33.0	39.0
11	37.138842962436456	39.0	37.0	39.0	34.0	39.0
12	37.14965155986835	39.0	37.0	39.0	34.0	39.0
13	37.046185700820494	39.0	37.0	39.0	33.0	39.0
14	38.25636231592935	40.0	37.0	41.0	34.0	41.0
15	38.318131248358235	40.0	37.0	41.0	34.0	41.0
16	38.27828082265865	40.0	37.0	41.0	34.0	41.0
17	38.16376686187555	40.0	37.0	41.0	33.0	41.0
18	38.07273204876617	40.0	37.0	41.0	33.0	41.0
19	37.94312931687192	40.0	37.0	41.0	33.0	41.0
20	38.027033082497645	40.0	37.0	41.0	34.0	41.0
21	38.02249022667923	40.0	37.0	41.0	34.0	41.0
22	38.014099850116665	40.0	37.0	41.0	33.0	41.0
23	37.98437041272	40.0	37.0	41.0	34.0	41.0
24	37.93249069023595	40.0	37.0	41.0	33.0	41.0
25	37.851074678986976	40.0	36.0	41.0	33.0	41.0
26	37.734938269697295	40.0	36.0	41.0	33.0	41.0
27	37.63847211706352	40.0	36.0	41.0	33.0	41.0
28	37.643262203130554	40.0	36.0	41.0	33.0	41.0
29	37.59767912604107	40.0	36.0	41.0	33.0	41.0
30	37.525341100483644	40.0	36.0	41.0	33.0	41.0
31	37.4162662051702	40.0	36.0	41.0	33.0	41.0
32	37.27077892980206	40.0	36.0	41.0	32.0	41.0
33	37.19685708546441	39.0	36.0	41.0	32.0	41.0
34	37.07814021045475	39.0	35.0	41.0	31.0	41.0
35	37.025997805831544	39.0	35.0	41.0	31.0	41.0
36	36.91504550581764	39.0	35.0	41.0	31.0	41.0
37	36.85253488264289	39.0	35.0	41.0	31.0	41.0
38	36.7090795308806	39.0	35.0	41.0	30.0	41.0
39	36.704652564241236	39.0	35.0	41.0	30.0	41.0
40	36.52254430829612	39.0	35.0	41.0	30.0	41.0
41	36.48966268522954	39.0	35.0	41.0	30.0	41.0
42	36.44676051114854	39.0	35.0	40.0	30.0	41.0
43	36.425993170264384	39.0	35.0	40.0	30.0	41.0
44	36.22558987592132	39.0	35.0	40.0	30.0	41.0
45	36.117967458318525	39.0	35.0	40.0	29.0	41.0
46	36.01300276588841	38.0	35.0	40.0	29.0	41.0
47	35.860546687887265	38.0	35.0	40.0	28.0	41.0
48	35.79333096404345	38.0	35.0	40.0	28.0	41.0
49	35.626033345179785	38.0	35.0	40.0	27.0	41.0
50	35.4620965125083	38.0	34.0	40.0	27.0	41.0
51	33.213174281873385	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	7.0
10	8.0
11	2.0
12	1.0
13	3.0
14	5.0
15	7.0
16	20.0
17	29.0
18	46.0
19	68.0
20	110.0
21	185.0
22	308.0
23	445.0
24	596.0
25	794.0
26	1039.0
27	1352.0
28	1561.0
29	1871.0
30	2387.0
31	3143.0
32	4145.0
33	5659.0
34	8629.0
35	11039.0
36	12057.0
37	19388.0
38	25682.0
39	28837.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08348656458118	20.595824899176414	26.845342027597074	11.475346508645332
2	28.986201461748845	28.055225056785698	27.00140612203903	15.957167359426425
3	30.14586584668634	26.85229537833954	27.61561877095663	15.386220004017492
4	27.819583726068885	25.840969142574593	29.332323809818135	17.00712332153839
5	26.821391597261922	30.260982431200457	24.634176491493733	18.283449480043885
6	25.91282043358005	36.871301203702274	23.282908663875023	13.932969698842653
7	61.89254755319313	27.760866542021418	6.528423752646137	3.818162152139314
8	83.83500471282662	6.566280884466215	6.383175981581346	3.215538421125825
9	76.46058995318077	9.278860268553858	7.569108580434817	6.691441197830554
10	33.91767232720924	40.38815149033484	13.858028029729438	11.836148152726485
11	32.71937821592471	24.831188095863528	24.410896673207965	18.038537015003786
12	31.086885980499712	23.00168425606873	27.251726748767712	18.659703014663844
13	25.89118778682572	25.83633357541295	29.625909730055472	18.646568907705856
14	20.64218057079284	29.922586028400577	27.095662654325757	22.339570746480835
15	19.907443175672544	26.356289692043823	34.28697251108673	19.449294621196902
16	23.157748350510683	25.882689247029372	29.47448120277516	21.48508119968478
17	22.04212185360879	25.121683637993108	28.706522242996428	24.129672265401673
18	23.476829890137058	25.973082806681397	29.696988426533988	20.853098876647557
19	23.830678183475747	27.63493363413014	26.41500687609129	22.119381306302827
20	24.771698317289122	27.3088987437613	28.37121621830431	19.548186720645273
21	23.77582397206298	27.878300910116355	28.430705996878718	19.915169120941947
22	22.820897136764685	25.044424185299068	27.719919032093575	24.41475964584267
23	21.12659733918445	28.647032464422022	28.51337361126134	21.712996585132192
24	22.718142064681615	25.719671801844957	28.552003337608355	23.010182795865074
25	20.414265185345425	29.065778698023703	28.427615618770957	22.092340497859915
26	19.789236213050668	30.094874607908277	27.003723905619854	23.112165273421205
27	22.10315682123708	28.728154889750762	27.298082420384134	21.870605868628026
28	19.739017568799543	27.73150794999768	30.20690081431463	22.322573666888143
29	21.948637915849005	27.559991965016923	28.405982972016623	22.085387147117448
30	22.705007957723627	27.930064743421358	27.805677024583957	21.559250274271058
31	23.79745661881731	27.334394363150334	25.620007107869647	23.24814191016271
32	24.611771250212463	29.29214889441723	25.906639677364524	20.18944017800578
33	23.07121776349336	27.70369454702783	25.63314121482763	23.591946474651174
34	21.152865553100423	27.84971491261956	28.76292164346308	22.234497890816943
35	22.463958465318232	28.134029698533613	27.129656813511133	22.27235502263702
36	22.878069131758274	27.73459832810544	27.09720784337964	22.290124696756646
37	22.650926340837803	28.55895668835082	27.627207688860732	21.162909281950647
38	20.640635381738957	30.009116615417895	25.546610627810313	23.803637375032835
39	22.43769025140226	26.865429485297526	28.342630220807514	22.354250042492698
40	21.394687640032757	28.20665358406601	28.649350248002843	21.74930852789839
41	19.637035091243412	27.4773243506343	28.27927746959841	24.60636308852388
42	20.93653908555712	27.172149512492854	28.10158072840212	23.78973067354791
43	22.89892918398566	25.6037826228039	26.74799511720259	24.74929307600785
44	21.7724863637066	26.89015251015962	28.0992629448213	23.238098181312484
45	20.884002657725173	27.39156635814392	26.923374074818053	24.801056909312855
46	22.41605760464793	28.932892439389956	26.898651049955962	21.75239890600615
47	20.393405133118037	26.738723982879304	30.648824883724522	22.219046000278134
48	20.89250119752152	26.676143826197134	28.592950847536198	23.838404128745154
49	20.493069827093343	24.925444628150252	30.547615000695334	24.033870544061063
50	20.224979526245036	25.959948699723412	29.533970981349565	24.281100792681983
51	20.67231175734351	25.976173184789157	27.048534388182393	26.302980669684935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	148.0
2	227.0
3	431.5
4	636.0
5	473.0
6	310.0
7	287.5
8	265.0
9	235.5
10	206.0
11	238.0
12	270.0
13	257.0
14	244.0
15	251.5
16	259.0
17	249.0
18	239.0
19	255.5
20	272.0
21	301.5
22	331.0
23	380.5
24	430.0
25	699.0
26	1197.0
27	1426.0
28	1681.0
29	1936.0
30	2228.5
31	2521.0
32	2824.5
33	3128.0
34	3627.0
35	4126.0
36	4562.5
37	4999.0
38	5436.5
39	5874.0
40	6446.0
41	7018.0
42	7892.5
43	8767.0
44	9588.5
45	10410.0
46	12251.5
47	14093.0
48	12821.0
49	11549.0
50	11095.5
51	10642.0
52	9224.0
53	7806.0
54	7114.5
55	6423.0
56	5920.5
57	5418.0
58	5105.5
59	4793.0
60	4605.5
61	4418.0
62	3793.5
63	3169.0
64	2766.0
65	2363.0
66	1941.0
67	1519.0
68	1190.0
69	861.0
70	722.0
71	583.0
72	480.0
73	377.0
74	290.0
75	160.5
76	118.0
77	95.0
78	72.0
79	54.5
80	37.0
81	27.0
82	17.0
83	19.5
84	22.0
85	14.0
86	6.0
87	5.5
88	5.0
89	4.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	129434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.04020581918198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12618634106977	30.443314739558385
2	9.84509587214708	8.671600970378726
3	5.380418574460993	7.108642242378354
4	3.7348911460800305	6.579414991424201
5	2.708629370384015	5.964429747979665
6	2.0893637176990687	5.520960489515892
7	1.552549865796537	4.7862230943955995
8	1.2560742417065769	4.425421450314445
9	0.9771415539533007	3.8730163635520807
>10	3.2822833885935827	19.00350757915231
>50	0.028068698138694458	0.8676236537540368
>100	0.014034349069347229	1.1256702257521207
>500	0.005262880901005211	1.6301744518441832
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	793	0.6126674598637144	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	673	0.5199561166308698	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	644	0.497550875349599	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.21787165659718466	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	261	0.20164717153143685	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	202	0.156064094441955	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCT	175	0.13520404221456495	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	162	0.12516031336434014	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	147	0.11357139546023455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.725945269403711E-4	0.0	0.0	0.10120988302918862	0.0
2	7.725945269403711E-4	0.0	0.0	0.6173030270253566	0.0
3	7.725945269403711E-4	0.0	0.0	0.7810930667367152	0.0
4	7.725945269403711E-4	0.0	0.0	1.0746789869740563	0.0
5	7.725945269403711E-4	0.0	0.0	2.2119381306302826	0.0
6	7.725945269403711E-4	0.0	0.0	2.547244155322404	0.0
7	7.725945269403711E-4	0.0	0.0	3.0270253565523744	0.0
8	7.725945269403711E-4	0.0	0.0	3.8289784755164793	0.0
9	7.725945269403711E-4	0.0	0.0	4.135698502711807	0.0
10	7.725945269403711E-4	0.0	0.0	5.657709720784338	0.0
11	7.725945269403711E-4	0.0	0.0	6.511426673053448	0.0
12	7.725945269403711E-4	0.0	0.0	7.878146391210965	0.0
13	7.725945269403711E-4	0.0	0.0	8.184866418406292	0.0
14	7.725945269403711E-4	0.0	0.0	8.308481542716752	0.0
15	7.725945269403711E-4	0.0	0.0	8.593568923157749	0.0
16	7.725945269403711E-4	0.0	0.0	9.065624179118315	0.0
17	7.725945269403711E-4	0.0	0.0	9.653568614119937	0.0
18	7.725945269403711E-4	0.0	0.0	10.328816230665822	0.0
19	7.725945269403711E-4	0.0	0.0	10.671075606100406	0.0
20	7.725945269403711E-4	0.0	0.0	11.061235842205294	0.0
21	7.725945269403711E-4	0.0	0.0	11.498524344453545	0.0
22	7.725945269403711E-4	0.0	0.0	12.026206406353817	0.0
23	7.725945269403711E-4	0.0	0.0	12.473538637452291	0.0
24	7.725945269403711E-4	0.0	0.0	12.835885470587327	0.0
25	7.725945269403711E-4	0.0	0.0	13.148786253998177	0.0
26	7.725945269403711E-4	0.0	0.0	13.433873634439173	0.0
27	7.725945269403711E-4	0.0	0.0	13.769952253658236	0.0
28	7.725945269403711E-4	0.0	0.0	14.095987144027072	0.0
29	7.725945269403711E-4	0.0	0.0	14.42588500703061	0.0
30	7.725945269403711E-4	0.0	0.0	14.831497133674304	0.0
31	7.725945269403711E-4	0.0	0.0	15.226292936940835	0.0
32	7.725945269403711E-4	0.0	0.0	15.604864255141617	0.0
33	7.725945269403711E-4	0.0	0.0	15.957167359426427	0.0
34	7.725945269403711E-4	0.0	0.0	16.296336356753248	0.0
35	7.725945269403711E-4	0.0	0.0	16.66949951326545	0.0
36	7.725945269403711E-4	0.0	0.0	17.02721077923884	0.0
37	7.725945269403711E-4	0.0	0.0	17.389557612373874	0.0
38	7.725945269403711E-4	0.0	0.0	17.78280822658652	0.0
39	0.0015451890538807423	0.0	0.0	18.138974303506036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	45	3.783498E-10	45.000004	7
CTAGATG	20	7.0100784E-4	45.0	1
CTCACGT	25	3.87158E-5	45.0	45
GGACTAC	20	7.0100784E-4	45.0	8
GGATCGC	20	7.0100784E-4	45.0	8
CACGACG	25	3.87158E-5	45.0	26
TACGGCT	100	0.0	45.0	7
CATAGGA	20	7.0100784E-4	45.0	4
CTGTTAT	20	7.0100784E-4	45.0	23
CTACCCA	20	7.0100784E-4	45.0	16
CGATGGC	20	7.0100784E-4	45.0	11
CGGTCTA	25	3.87158E-5	45.0	31
GGCGATA	40	6.7338988E-9	45.0	8
GGACAAC	40	6.7338988E-9	45.0	8
CCCGGGA	20	7.0100784E-4	45.0	4
TCGACAC	25	3.87158E-5	45.0	34
CGTTTGG	20	7.0100784E-4	45.0	2
GACCGAT	115	0.0	41.08696	9
GGACTCC	40	3.4266122E-7	39.375	8
GGATGAC	70	0.0	38.571426	8
>>END_MODULE