##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936393.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60684 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954666798497133 33.0 31.0 34.0 30.0 34.0 2 32.06011469250544 33.0 31.0 34.0 30.0 34.0 3 31.89132225957419 33.0 31.0 34.0 30.0 34.0 4 35.695834157273744 37.0 35.0 37.0 35.0 37.0 5 35.81153187001516 37.0 35.0 37.0 35.0 37.0 6 35.81487706809044 37.0 35.0 37.0 35.0 37.0 7 26.921626787950697 36.0 10.0 37.0 0.0 37.0 8 31.248137894667458 36.0 19.0 37.0 17.0 37.0 9 36.029249884648344 39.0 32.0 39.0 32.0 39.0 10 36.76397402939819 38.0 35.0 39.0 33.0 39.0 11 37.0684694482895 39.0 37.0 39.0 34.0 39.0 12 37.02821171972843 39.0 35.0 39.0 34.0 39.0 13 36.886230307824135 39.0 35.0 39.0 33.0 39.0 14 37.99939028409465 40.0 37.0 41.0 33.0 41.0 15 38.12522246391141 40.0 37.0 41.0 33.0 41.0 16 38.090995979170785 40.0 37.0 41.0 34.0 41.0 17 38.01580317711423 40.0 36.0 41.0 33.0 41.0 18 37.90910289367873 40.0 36.0 41.0 33.0 41.0 19 37.80375387251994 39.0 36.0 41.0 33.0 41.0 20 37.81393777602004 39.0 36.0 41.0 33.0 41.0 21 37.7636938896579 39.0 36.0 41.0 33.0 41.0 22 37.79640432403929 39.0 36.0 41.0 33.0 41.0 23 37.75004943642476 39.0 35.0 41.0 33.0 41.0 24 37.71045086019379 39.0 35.0 41.0 33.0 41.0 25 37.618070661129785 39.0 35.0 41.0 33.0 41.0 26 37.49668775954123 39.0 35.0 41.0 33.0 41.0 27 37.39066310724409 39.0 35.0 41.0 33.0 41.0 28 37.39847406235581 39.0 35.0 41.0 33.0 41.0 29 37.418973699822025 39.0 35.0 41.0 33.0 41.0 30 37.33447037110276 39.0 35.0 41.0 33.0 41.0 31 37.13445059653286 39.0 35.0 41.0 32.0 41.0 32 36.993985234987804 39.0 35.0 41.0 32.0 41.0 33 36.86872981345989 39.0 35.0 41.0 31.0 41.0 34 36.657636279744246 39.0 35.0 40.0 31.0 41.0 35 36.63567002834355 39.0 35.0 40.0 31.0 41.0 36 36.48933821106058 39.0 35.0 40.0 31.0 41.0 37 36.34623624019511 39.0 35.0 40.0 30.0 41.0 38 36.20053061762573 38.0 35.0 40.0 30.0 41.0 39 36.24851690725727 38.0 35.0 40.0 30.0 41.0 40 35.97617164326676 38.0 35.0 40.0 30.0 41.0 41 36.0439325028014 38.0 35.0 40.0 30.0 41.0 42 35.92495550721772 38.0 35.0 40.0 30.0 41.0 43 35.8617757563773 38.0 35.0 40.0 30.0 41.0 44 35.61147913782875 38.0 35.0 40.0 29.0 41.0 45 35.519444993738055 38.0 35.0 40.0 28.0 41.0 46 35.47859402807989 38.0 35.0 40.0 28.0 41.0 47 35.33158657965856 38.0 34.0 40.0 27.0 41.0 48 35.20992353832971 38.0 34.0 40.0 26.0 41.0 49 34.991875947531476 38.0 34.0 40.0 26.0 41.0 50 34.6697152461934 37.0 34.0 40.0 24.0 41.0 51 32.331421791576034 35.0 30.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 5.0 11 2.0 12 1.0 13 1.0 14 2.0 15 4.0 16 5.0 17 7.0 18 15.0 19 28.0 20 66.0 21 102.0 22 140.0 23 213.0 24 333.0 25 477.0 26 577.0 27 685.0 28 812.0 29 989.0 30 1174.0 31 1501.0 32 2012.0 33 2970.0 34 4911.0 35 6381.0 36 5820.0 37 8959.0 38 11112.0 39 11375.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.17315931711819 21.705886230307826 27.038428580845032 13.082525871728956 2 30.184233076263922 24.955507217718015 29.08674444664162 15.773515259376442 3 29.707995517764157 24.330960384944962 30.713202821171976 15.247841276118912 4 27.949706677213104 26.09419286797179 29.953529760727704 16.002570694087403 5 24.876408938105595 30.17599367213763 27.66956693691912 17.27803045283765 6 25.949179355349024 34.407751631402014 25.575110408015295 14.067958605233668 7 61.645573792103356 27.252653088128664 7.074352382835674 4.027420736932305 8 82.67582888405511 4.670094258783205 8.099334256146594 4.554742601015095 9 76.59020499637467 7.679124645705622 10.426141981411904 5.304528376507811 10 39.67108298727836 33.297079955177644 15.26267220354624 11.769164853997758 11 33.19326346318634 23.680047458967767 26.779711291279412 16.346977786566473 12 31.20591918792433 22.065124250214225 28.41605695076132 18.312899611100125 13 23.49548480653879 27.677806341045414 31.420143695207965 17.406565157207833 14 18.70674312833696 31.647551249093663 29.85300903038692 19.792696592182455 15 18.04923867905873 25.810757366027286 39.015226418825385 17.12477753608859 16 20.687495880297938 24.787423373541625 35.281128468789134 19.2439522773713 17 20.351328191945157 22.971458704106517 29.719530683540967 26.95768242040736 18 22.599037637598048 24.508931514072902 32.309999340847675 20.58203150748138 19 22.040406037835343 26.588557115549406 29.5184892228594 21.85254762375585 20 24.74128271043438 26.56713466482104 29.516841342034144 19.174741282710432 21 22.852811284687892 26.804429503658294 30.759343484279217 19.583415727374597 22 21.272493573264782 24.528706083976008 29.68986882868631 24.508931514072902 23 19.255487443148112 27.761848263133608 28.87911146265902 24.103552831059257 24 22.556192736141323 24.034341836398394 31.029595939621647 22.37986948783864 25 18.58974358974359 26.227671214817743 30.274866521653156 24.90771867378551 26 17.810295959396218 27.83929866192077 32.25561927361413 22.09478610506888 27 20.806143299716563 27.272427658031773 31.031243820446907 20.890185221804757 28 16.770483158657967 25.87502471821238 35.505240261024326 21.84925186210533 29 19.881682156746425 24.312833695867113 34.82466548019247 20.980818667193986 30 22.735811746094523 24.90936655461077 31.827170259046866 20.52765144024784 31 23.938764748533387 26.278755520400765 28.755520400764617 21.02695933030123 32 26.1815305517105 27.147188715312108 28.271043438138555 18.400237294838835 33 25.43833629951882 26.03981280073825 28.897238151736865 19.624612748006065 34 20.745171709181992 27.06149891239866 31.795860523366947 20.397468855052402 35 22.84127611891108 25.698701469909697 30.469316459033685 20.990705952145543 36 25.853602267484018 23.961835080087006 31.818930854920573 18.365631797508406 37 24.047524883000463 26.169995385933692 31.144947597389756 18.637532133676093 38 23.576230966976468 26.642937182782937 28.719267022608925 21.061564827631667 39 24.39687561795531 24.62757893349153 30.37373937116868 20.60180607738448 40 23.633906795860522 25.278491859468723 30.317711423109884 20.76988992156087 41 22.18541955045811 24.291411245138754 29.073561400039548 24.449607804363588 42 24.253509986157802 23.86955375387252 29.5465031968888 22.33043306308088 43 25.283435501944503 23.132951024981875 29.806868367279677 21.77674510579395 44 21.203282578603915 26.45178300705293 30.232021620196427 22.112912794146727 45 21.19833893612814 26.278755520400765 29.144420275525672 23.378485267945422 46 24.352382835673325 25.509195175004944 29.800276843978647 20.33814514534309 47 20.41065190165447 26.08760134467075 32.425350998615784 21.076395755058993 48 24.01127150484477 22.526530881286668 30.6324566607343 22.82974095313427 49 22.27275723419682 21.55757695603454 33.715641684793354 22.454024124975284 50 21.214817744380728 23.386724672071715 32.71043438138554 22.688023202162018 51 20.878650056027947 23.991496934941665 29.648671808054843 25.481181200975545 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61.0 1 95.5 2 130.0 3 297.5 4 465.0 5 324.5 6 184.0 7 169.5 8 155.0 9 171.5 10 188.0 11 181.5 12 175.0 13 180.0 14 185.0 15 181.5 16 178.0 17 193.0 18 208.0 19 226.0 20 244.0 21 273.0 22 302.0 23 366.5 24 431.0 25 435.5 26 502.0 27 564.0 28 709.5 29 855.0 30 953.5 31 1052.0 32 1277.5 33 1503.0 34 1604.0 35 1705.0 36 1805.5 37 1906.0 38 2006.5 39 2107.0 40 2575.0 41 3043.0 42 3726.0 43 4409.0 44 4900.0 45 5391.0 46 5873.0 47 6355.0 48 6474.5 49 6594.0 50 6302.5 51 6011.0 52 4991.5 53 3972.0 54 3343.5 55 2715.0 56 2490.5 57 2266.0 58 2128.0 59 1990.0 60 1826.0 61 1662.0 62 1472.0 63 1282.0 64 1069.0 65 856.0 66 671.5 67 487.0 68 390.5 69 294.0 70 201.5 71 109.0 72 90.5 73 72.0 74 68.0 75 43.0 76 22.0 77 20.0 78 18.0 79 16.0 80 14.0 81 9.5 82 5.0 83 4.5 84 4.0 85 5.0 86 6.0 87 4.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 60684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.51110671676224 #Duplication Level Percentage of deduplicated Percentage of total 1 72.88460923254104 37.543668841869355 2 10.086695031830832 10.39153648408147 3 5.0033590325986115 7.731856832113901 4 3.3590325986116 6.921099466086613 5 2.2137624364183117 5.701667655395162 6 1.602738411337535 4.9535297607277045 7 1.2700342301417191 4.579460813393975 8 0.831760452989539 3.4275921165381322 9 0.7325890143638633 3.3962823808582163 >10 1.9866278511788606 13.55381978775295 >50 0.015995393326721903 0.6212510711225364 >100 0.012796314661377525 1.178234790059983 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.37571682815898755 No Hit CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC 188 0.3098015951486388 Illumina Single End Adapter 2 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC 166 0.27354821699294707 Illumina Single End Adapter 2 (95% over 21bp) CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT 133 0.2191681497594094 Illumina Single End Adapter 2 (95% over 22bp) GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG 96 0.15819655922483686 No Hit TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC 81 0.1334783468459561 Illumina Single End Adapter 2 (95% over 21bp) GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC 75 0.12359106189440379 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC 66 0.10876013446707535 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3130973567991563 0.0 2 0.0 0.0 0.0 1.415529628897238 0.0 3 0.0 0.0 0.0 1.853865928416057 0.0 4 0.0 0.0 0.0 2.2872585854590994 0.0 5 0.0 0.0 0.0 3.5577747017335706 0.0 6 0.0 0.0 0.0 4.16254696460352 0.0 7 0.0 0.0 0.0 4.824995056357524 0.0 8 0.0 0.0 0.0 6.286665348362006 0.0 9 0.0 0.0 0.0 6.8189308549205725 0.0 10 0.0 0.0 0.0 8.348164260760662 0.0 11 0.0 0.0 0.0 9.826313361017732 0.0 12 0.0 0.0 0.0 11.18911080350669 0.0 13 0.0 0.0 0.0 11.655461077054907 0.0 14 0.0 0.0 0.0 11.853206776085953 0.0 15 0.0 0.0 0.0 12.187726583613474 0.0 16 0.0 0.0 0.0 12.949047524883001 0.0 17 0.0 0.0 0.0 13.804297673192275 0.0 18 0.0 0.0 0.0 14.845758354755784 0.0 19 0.0 0.0 0.0 15.396150550392196 0.0 20 0.0 0.0 0.0 15.921824533649726 0.0 21 0.0 0.0 0.0 16.620526003559423 0.0 22 0.0 0.0 0.0 17.342297805022742 0.0 23 0.0 0.0 0.0 18.069013248961834 0.0 24 0.0 0.0 0.0 18.579856304792038 0.0 25 0.0 0.0 0.0 19.070924790719136 0.0 26 0.0 0.0 0.0 19.505965328587436 0.0 27 0.0 0.0 0.0 19.927822819853667 0.0 28 0.0 0.0 0.0 20.318370575439985 0.0 29 0.0 0.0 0.0 20.745171709181992 0.0 30 0.0 0.0 0.0 21.242831718410123 0.0 31 0.0 0.0 0.0 21.755322655065587 0.0 32 0.0 0.0 0.0 22.195306835409664 0.0 33 0.0 0.0 0.0 22.679783798035725 0.0 34 0.0 0.0 0.0 23.184035330564893 0.0 35 0.0 0.0 0.0 23.620723749258453 0.0 36 0.0 0.0 0.0 24.06565157207831 0.0 37 0.0 0.0 0.0 24.507283633247642 0.0 38 0.0 0.0 0.0 24.963746621844308 0.0 39 0.0 0.0 0.0 25.40867444466416 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGATT 25 3.8471233E-5 45.000004 6 GGCCTTT 25 3.8471233E-5 45.000004 8 TAGATGG 25 3.8471233E-5 45.000004 2 GAGTGAC 20 6.9805066E-4 45.0 9 TACGGCG 20 6.9805066E-4 45.0 15 AGGCGAT 20 6.9805066E-4 45.0 7 GGATAGC 20 6.9805066E-4 45.0 8 CTGGCAC 20 6.9805066E-4 45.0 26 CGGGAGC 20 6.9805066E-4 45.0 6 AGGGGTA 45 1.8770152E-8 40.0 6 AGGGGTT 40 3.3865763E-7 39.375 6 AGGGAGC 40 3.3865763E-7 39.375 6 ATCTCTT 35 6.150989E-6 38.57143 7 ATTGGGA 70 0.0 38.57143 4 GAACGGG 35 6.150989E-6 38.57143 3 TATCTCT 35 6.150989E-6 38.57143 6 AACGGGA 35 6.150989E-6 38.57143 4 GTATGGG 65 9.094947E-12 38.076927 3 TACGGGA 30 1.1272224E-4 37.500004 4 CTAGGAT 30 1.1272224E-4 37.500004 5 >>END_MODULE