##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60684
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954666798497133	33.0	31.0	34.0	30.0	34.0
2	32.06011469250544	33.0	31.0	34.0	30.0	34.0
3	31.89132225957419	33.0	31.0	34.0	30.0	34.0
4	35.695834157273744	37.0	35.0	37.0	35.0	37.0
5	35.81153187001516	37.0	35.0	37.0	35.0	37.0
6	35.81487706809044	37.0	35.0	37.0	35.0	37.0
7	26.921626787950697	36.0	10.0	37.0	0.0	37.0
8	31.248137894667458	36.0	19.0	37.0	17.0	37.0
9	36.029249884648344	39.0	32.0	39.0	32.0	39.0
10	36.76397402939819	38.0	35.0	39.0	33.0	39.0
11	37.0684694482895	39.0	37.0	39.0	34.0	39.0
12	37.02821171972843	39.0	35.0	39.0	34.0	39.0
13	36.886230307824135	39.0	35.0	39.0	33.0	39.0
14	37.99939028409465	40.0	37.0	41.0	33.0	41.0
15	38.12522246391141	40.0	37.0	41.0	33.0	41.0
16	38.090995979170785	40.0	37.0	41.0	34.0	41.0
17	38.01580317711423	40.0	36.0	41.0	33.0	41.0
18	37.90910289367873	40.0	36.0	41.0	33.0	41.0
19	37.80375387251994	39.0	36.0	41.0	33.0	41.0
20	37.81393777602004	39.0	36.0	41.0	33.0	41.0
21	37.7636938896579	39.0	36.0	41.0	33.0	41.0
22	37.79640432403929	39.0	36.0	41.0	33.0	41.0
23	37.75004943642476	39.0	35.0	41.0	33.0	41.0
24	37.71045086019379	39.0	35.0	41.0	33.0	41.0
25	37.618070661129785	39.0	35.0	41.0	33.0	41.0
26	37.49668775954123	39.0	35.0	41.0	33.0	41.0
27	37.39066310724409	39.0	35.0	41.0	33.0	41.0
28	37.39847406235581	39.0	35.0	41.0	33.0	41.0
29	37.418973699822025	39.0	35.0	41.0	33.0	41.0
30	37.33447037110276	39.0	35.0	41.0	33.0	41.0
31	37.13445059653286	39.0	35.0	41.0	32.0	41.0
32	36.993985234987804	39.0	35.0	41.0	32.0	41.0
33	36.86872981345989	39.0	35.0	41.0	31.0	41.0
34	36.657636279744246	39.0	35.0	40.0	31.0	41.0
35	36.63567002834355	39.0	35.0	40.0	31.0	41.0
36	36.48933821106058	39.0	35.0	40.0	31.0	41.0
37	36.34623624019511	39.0	35.0	40.0	30.0	41.0
38	36.20053061762573	38.0	35.0	40.0	30.0	41.0
39	36.24851690725727	38.0	35.0	40.0	30.0	41.0
40	35.97617164326676	38.0	35.0	40.0	30.0	41.0
41	36.0439325028014	38.0	35.0	40.0	30.0	41.0
42	35.92495550721772	38.0	35.0	40.0	30.0	41.0
43	35.8617757563773	38.0	35.0	40.0	30.0	41.0
44	35.61147913782875	38.0	35.0	40.0	29.0	41.0
45	35.519444993738055	38.0	35.0	40.0	28.0	41.0
46	35.47859402807989	38.0	35.0	40.0	28.0	41.0
47	35.33158657965856	38.0	34.0	40.0	27.0	41.0
48	35.20992353832971	38.0	34.0	40.0	26.0	41.0
49	34.991875947531476	38.0	34.0	40.0	26.0	41.0
50	34.6697152461934	37.0	34.0	40.0	24.0	41.0
51	32.331421791576034	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	2.0
12	1.0
13	1.0
14	2.0
15	4.0
16	5.0
17	7.0
18	15.0
19	28.0
20	66.0
21	102.0
22	140.0
23	213.0
24	333.0
25	477.0
26	577.0
27	685.0
28	812.0
29	989.0
30	1174.0
31	1501.0
32	2012.0
33	2970.0
34	4911.0
35	6381.0
36	5820.0
37	8959.0
38	11112.0
39	11375.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.17315931711819	21.705886230307826	27.038428580845032	13.082525871728956
2	30.184233076263922	24.955507217718015	29.08674444664162	15.773515259376442
3	29.707995517764157	24.330960384944962	30.713202821171976	15.247841276118912
4	27.949706677213104	26.09419286797179	29.953529760727704	16.002570694087403
5	24.876408938105595	30.17599367213763	27.66956693691912	17.27803045283765
6	25.949179355349024	34.407751631402014	25.575110408015295	14.067958605233668
7	61.645573792103356	27.252653088128664	7.074352382835674	4.027420736932305
8	82.67582888405511	4.670094258783205	8.099334256146594	4.554742601015095
9	76.59020499637467	7.679124645705622	10.426141981411904	5.304528376507811
10	39.67108298727836	33.297079955177644	15.26267220354624	11.769164853997758
11	33.19326346318634	23.680047458967767	26.779711291279412	16.346977786566473
12	31.20591918792433	22.065124250214225	28.41605695076132	18.312899611100125
13	23.49548480653879	27.677806341045414	31.420143695207965	17.406565157207833
14	18.70674312833696	31.647551249093663	29.85300903038692	19.792696592182455
15	18.04923867905873	25.810757366027286	39.015226418825385	17.12477753608859
16	20.687495880297938	24.787423373541625	35.281128468789134	19.2439522773713
17	20.351328191945157	22.971458704106517	29.719530683540967	26.95768242040736
18	22.599037637598048	24.508931514072902	32.309999340847675	20.58203150748138
19	22.040406037835343	26.588557115549406	29.5184892228594	21.85254762375585
20	24.74128271043438	26.56713466482104	29.516841342034144	19.174741282710432
21	22.852811284687892	26.804429503658294	30.759343484279217	19.583415727374597
22	21.272493573264782	24.528706083976008	29.68986882868631	24.508931514072902
23	19.255487443148112	27.761848263133608	28.87911146265902	24.103552831059257
24	22.556192736141323	24.034341836398394	31.029595939621647	22.37986948783864
25	18.58974358974359	26.227671214817743	30.274866521653156	24.90771867378551
26	17.810295959396218	27.83929866192077	32.25561927361413	22.09478610506888
27	20.806143299716563	27.272427658031773	31.031243820446907	20.890185221804757
28	16.770483158657967	25.87502471821238	35.505240261024326	21.84925186210533
29	19.881682156746425	24.312833695867113	34.82466548019247	20.980818667193986
30	22.735811746094523	24.90936655461077	31.827170259046866	20.52765144024784
31	23.938764748533387	26.278755520400765	28.755520400764617	21.02695933030123
32	26.1815305517105	27.147188715312108	28.271043438138555	18.400237294838835
33	25.43833629951882	26.03981280073825	28.897238151736865	19.624612748006065
34	20.745171709181992	27.06149891239866	31.795860523366947	20.397468855052402
35	22.84127611891108	25.698701469909697	30.469316459033685	20.990705952145543
36	25.853602267484018	23.961835080087006	31.818930854920573	18.365631797508406
37	24.047524883000463	26.169995385933692	31.144947597389756	18.637532133676093
38	23.576230966976468	26.642937182782937	28.719267022608925	21.061564827631667
39	24.39687561795531	24.62757893349153	30.37373937116868	20.60180607738448
40	23.633906795860522	25.278491859468723	30.317711423109884	20.76988992156087
41	22.18541955045811	24.291411245138754	29.073561400039548	24.449607804363588
42	24.253509986157802	23.86955375387252	29.5465031968888	22.33043306308088
43	25.283435501944503	23.132951024981875	29.806868367279677	21.77674510579395
44	21.203282578603915	26.45178300705293	30.232021620196427	22.112912794146727
45	21.19833893612814	26.278755520400765	29.144420275525672	23.378485267945422
46	24.352382835673325	25.509195175004944	29.800276843978647	20.33814514534309
47	20.41065190165447	26.08760134467075	32.425350998615784	21.076395755058993
48	24.01127150484477	22.526530881286668	30.6324566607343	22.82974095313427
49	22.27275723419682	21.55757695603454	33.715641684793354	22.454024124975284
50	21.214817744380728	23.386724672071715	32.71043438138554	22.688023202162018
51	20.878650056027947	23.991496934941665	29.648671808054843	25.481181200975545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	95.5
2	130.0
3	297.5
4	465.0
5	324.5
6	184.0
7	169.5
8	155.0
9	171.5
10	188.0
11	181.5
12	175.0
13	180.0
14	185.0
15	181.5
16	178.0
17	193.0
18	208.0
19	226.0
20	244.0
21	273.0
22	302.0
23	366.5
24	431.0
25	435.5
26	502.0
27	564.0
28	709.5
29	855.0
30	953.5
31	1052.0
32	1277.5
33	1503.0
34	1604.0
35	1705.0
36	1805.5
37	1906.0
38	2006.5
39	2107.0
40	2575.0
41	3043.0
42	3726.0
43	4409.0
44	4900.0
45	5391.0
46	5873.0
47	6355.0
48	6474.5
49	6594.0
50	6302.5
51	6011.0
52	4991.5
53	3972.0
54	3343.5
55	2715.0
56	2490.5
57	2266.0
58	2128.0
59	1990.0
60	1826.0
61	1662.0
62	1472.0
63	1282.0
64	1069.0
65	856.0
66	671.5
67	487.0
68	390.5
69	294.0
70	201.5
71	109.0
72	90.5
73	72.0
74	68.0
75	43.0
76	22.0
77	20.0
78	18.0
79	16.0
80	14.0
81	9.5
82	5.0
83	4.5
84	4.0
85	5.0
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	60684.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.51110671676224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88460923254104	37.543668841869355
2	10.086695031830832	10.39153648408147
3	5.0033590325986115	7.731856832113901
4	3.3590325986116	6.921099466086613
5	2.2137624364183117	5.701667655395162
6	1.602738411337535	4.9535297607277045
7	1.2700342301417191	4.579460813393975
8	0.831760452989539	3.4275921165381322
9	0.7325890143638633	3.3962823808582163
>10	1.9866278511788606	13.55381978775295
>50	0.015995393326721903	0.6212510711225364
>100	0.012796314661377525	1.178234790059983
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	228	0.37571682815898755	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	188	0.3098015951486388	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	166	0.27354821699294707	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	133	0.2191681497594094	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	96	0.15819655922483686	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	81	0.1334783468459561	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	75	0.12359106189440379	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	66	0.10876013446707535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3130973567991563	0.0
2	0.0	0.0	0.0	1.415529628897238	0.0
3	0.0	0.0	0.0	1.853865928416057	0.0
4	0.0	0.0	0.0	2.2872585854590994	0.0
5	0.0	0.0	0.0	3.5577747017335706	0.0
6	0.0	0.0	0.0	4.16254696460352	0.0
7	0.0	0.0	0.0	4.824995056357524	0.0
8	0.0	0.0	0.0	6.286665348362006	0.0
9	0.0	0.0	0.0	6.8189308549205725	0.0
10	0.0	0.0	0.0	8.348164260760662	0.0
11	0.0	0.0	0.0	9.826313361017732	0.0
12	0.0	0.0	0.0	11.18911080350669	0.0
13	0.0	0.0	0.0	11.655461077054907	0.0
14	0.0	0.0	0.0	11.853206776085953	0.0
15	0.0	0.0	0.0	12.187726583613474	0.0
16	0.0	0.0	0.0	12.949047524883001	0.0
17	0.0	0.0	0.0	13.804297673192275	0.0
18	0.0	0.0	0.0	14.845758354755784	0.0
19	0.0	0.0	0.0	15.396150550392196	0.0
20	0.0	0.0	0.0	15.921824533649726	0.0
21	0.0	0.0	0.0	16.620526003559423	0.0
22	0.0	0.0	0.0	17.342297805022742	0.0
23	0.0	0.0	0.0	18.069013248961834	0.0
24	0.0	0.0	0.0	18.579856304792038	0.0
25	0.0	0.0	0.0	19.070924790719136	0.0
26	0.0	0.0	0.0	19.505965328587436	0.0
27	0.0	0.0	0.0	19.927822819853667	0.0
28	0.0	0.0	0.0	20.318370575439985	0.0
29	0.0	0.0	0.0	20.745171709181992	0.0
30	0.0	0.0	0.0	21.242831718410123	0.0
31	0.0	0.0	0.0	21.755322655065587	0.0
32	0.0	0.0	0.0	22.195306835409664	0.0
33	0.0	0.0	0.0	22.679783798035725	0.0
34	0.0	0.0	0.0	23.184035330564893	0.0
35	0.0	0.0	0.0	23.620723749258453	0.0
36	0.0	0.0	0.0	24.06565157207831	0.0
37	0.0	0.0	0.0	24.507283633247642	0.0
38	0.0	0.0	0.0	24.963746621844308	0.0
39	0.0	0.0	0.0	25.40867444466416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGATT	25	3.8471233E-5	45.000004	6
GGCCTTT	25	3.8471233E-5	45.000004	8
TAGATGG	25	3.8471233E-5	45.000004	2
GAGTGAC	20	6.9805066E-4	45.0	9
TACGGCG	20	6.9805066E-4	45.0	15
AGGCGAT	20	6.9805066E-4	45.0	7
GGATAGC	20	6.9805066E-4	45.0	8
CTGGCAC	20	6.9805066E-4	45.0	26
CGGGAGC	20	6.9805066E-4	45.0	6
AGGGGTA	45	1.8770152E-8	40.0	6
AGGGGTT	40	3.3865763E-7	39.375	6
AGGGAGC	40	3.3865763E-7	39.375	6
ATCTCTT	35	6.150989E-6	38.57143	7
ATTGGGA	70	0.0	38.57143	4
GAACGGG	35	6.150989E-6	38.57143	3
TATCTCT	35	6.150989E-6	38.57143	6
AACGGGA	35	6.150989E-6	38.57143	4
GTATGGG	65	9.094947E-12	38.076927	3
TACGGGA	30	1.1272224E-4	37.500004	4
CTAGGAT	30	1.1272224E-4	37.500004	5
>>END_MODULE