##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936392.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 144831 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.993944666542383 33.0 31.0 34.0 30.0 34.0 2 32.13015859864256 33.0 31.0 34.0 30.0 34.0 3 31.97626198811028 33.0 31.0 34.0 30.0 34.0 4 35.73886115541562 37.0 35.0 37.0 35.0 37.0 5 35.8289730789679 37.0 35.0 37.0 35.0 37.0 6 35.81696598103997 37.0 35.0 37.0 35.0 37.0 7 26.725397187066307 35.0 10.0 37.0 0.0 37.0 8 31.156050845468165 36.0 19.0 37.0 17.0 37.0 9 36.001118545062866 39.0 32.0 39.0 32.0 39.0 10 36.667205225400636 38.0 35.0 39.0 33.0 39.0 11 37.07500466060443 39.0 37.0 39.0 34.0 39.0 12 37.19873507743508 39.0 37.0 39.0 34.0 39.0 13 37.17148952917538 39.0 37.0 39.0 34.0 39.0 14 38.38201766196463 40.0 38.0 41.0 34.0 41.0 15 38.47583735526234 40.0 38.0 41.0 34.0 41.0 16 38.395488534913106 40.0 38.0 41.0 34.0 41.0 17 38.31873701072284 40.0 38.0 41.0 34.0 41.0 18 38.214739938272885 40.0 37.0 41.0 34.0 41.0 19 38.13970075467269 40.0 37.0 41.0 34.0 41.0 20 38.17970600216805 40.0 37.0 41.0 34.0 41.0 21 38.1272862853947 40.0 37.0 41.0 34.0 41.0 22 38.126961769234484 40.0 37.0 41.0 34.0 41.0 23 38.0619135406094 40.0 37.0 41.0 34.0 41.0 24 38.03301779315202 40.0 37.0 41.0 34.0 41.0 25 37.93055354171413 40.0 37.0 41.0 33.0 41.0 26 37.82061851399217 40.0 37.0 41.0 33.0 41.0 27 37.7087087709123 40.0 36.0 41.0 33.0 41.0 28 37.70035420593658 40.0 36.0 41.0 33.0 41.0 29 37.69820687559984 40.0 36.0 41.0 33.0 41.0 30 37.65483218371757 40.0 36.0 41.0 33.0 41.0 31 37.55409408206807 40.0 36.0 41.0 33.0 41.0 32 37.415470444863324 40.0 36.0 41.0 33.0 41.0 33 37.33927128860534 40.0 36.0 41.0 32.0 41.0 34 37.222997838860465 40.0 36.0 41.0 32.0 41.0 35 37.11531371046254 40.0 36.0 41.0 31.0 41.0 36 36.969868329294144 39.0 36.0 41.0 31.0 41.0 37 36.93268015825341 39.0 35.0 41.0 31.0 41.0 38 36.81808452610284 39.0 35.0 41.0 31.0 41.0 39 36.80118206737508 39.0 35.0 41.0 31.0 41.0 40 36.66967707189759 39.0 35.0 41.0 30.0 41.0 41 36.611643916012454 39.0 35.0 41.0 30.0 41.0 42 36.5097251279077 39.0 35.0 41.0 30.0 41.0 43 36.481616504753816 39.0 35.0 41.0 30.0 41.0 44 36.30010149760756 39.0 35.0 40.0 30.0 41.0 45 36.2426207096547 39.0 35.0 40.0 29.0 41.0 46 36.17467254938514 39.0 35.0 40.0 29.0 41.0 47 36.02475989256444 39.0 35.0 40.0 29.0 41.0 48 35.961541382715026 39.0 35.0 40.0 28.0 41.0 49 35.81812595369776 39.0 35.0 40.0 28.0 41.0 50 35.610856791708954 38.0 35.0 40.0 27.0 41.0 51 33.558216127762705 37.0 32.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 9.0 9 7.0 10 9.0 11 11.0 12 6.0 13 2.0 14 2.0 15 8.0 16 6.0 17 18.0 18 43.0 19 99.0 20 130.0 21 219.0 22 331.0 23 481.0 24 695.0 25 989.0 26 1292.0 27 1438.0 28 1694.0 29 2043.0 30 2550.0 31 3349.0 32 4084.0 33 5788.0 34 8853.0 35 11565.0 36 13690.0 37 22419.0 38 29581.0 39 33414.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.48321146715827 22.714059835256265 29.004840124006602 11.79788857357886 2 30.369879376652786 25.93298396061617 26.8657953062535 16.83134135647755 3 29.977007684818858 24.94355490192017 29.252024773701763 15.82741263955921 4 28.08929027625301 26.32102243304265 27.35740276598242 18.232284524721916 5 25.064385387106352 31.465639262312628 25.350926251976443 18.11904909860458 6 26.32516519253475 35.98124710869911 24.03007643391263 13.66351126485352 7 63.09767936422451 28.113456373290248 5.344850204721365 3.4440140577638765 8 86.91647506404016 4.2083531840559 6.022191381679336 2.8529803702246066 9 79.71428768702833 8.949741422761702 7.5936781490150596 3.7422927411949103 10 32.61456456145439 42.07731770132085 13.631059648832085 11.677058088392677 11 28.30402330992674 27.01976786737646 27.880080921902078 16.79612790079472 12 27.362235985389866 24.554825969578335 29.7256802756316 18.3572577694002 13 24.31385545912132 26.706299065807737 30.619825866009347 18.360019609061595 14 19.74991541866037 29.604849790445414 29.606921170191463 21.038313620702752 15 19.524825486256393 27.57351671948685 33.4790203754721 19.422637418784653 16 22.351568379697717 27.30354689258515 31.20533587422582 19.139548853491313 17 22.305998025284644 26.281666217867723 29.55651759637094 21.855818160476694 18 22.384019995719147 26.983863951778282 30.63294460440099 19.99917144810158 19 23.022004957502194 28.133479710835385 28.312999288826283 20.531516042836135 20 23.49289861977063 27.791702052737328 29.70427601825576 19.011123309236282 21 24.0797895478178 27.14197927239334 30.235239693159617 18.542991486629244 22 22.568372793117494 25.663704593629816 28.821177786523606 22.946744826729084 23 20.813914148214124 27.722656061202365 29.895533414807602 21.567896375775906 24 20.35406784459128 27.183406867314318 31.438711325613994 21.02381396248041 25 19.824485089518127 29.051791398250376 29.537875178656503 21.585848333574994 26 20.3181639289931 30.124075646788324 27.98226898937382 21.57549143484475 27 20.48180292893096 29.39218813651773 29.564803115355136 20.561205819196164 28 19.21135668468767 28.36271240273146 31.40004557035441 21.02588534222646 29 19.966719832080148 27.315975171061446 30.858724996720316 21.85858000013809 30 20.439684874094635 27.96293611174403 29.513709081619265 22.083669932542065 31 21.876531957937182 28.553279339367954 27.620467993730625 21.94972070896424 32 21.958696687863785 28.813582727454758 27.97260255055893 21.25511803412253 33 21.23647561640809 28.54499382038376 27.846938845965298 22.371591717242858 34 20.468684190539317 28.54499382038376 28.674800284469487 22.31152170460744 35 19.74922495874502 28.36133148290076 28.10655177413675 23.782891784217465 36 21.16535824512708 28.942008271709785 28.62301579081826 21.269617692344873 37 20.34025864628429 28.857081702121786 28.95443655018608 21.84822310140785 38 20.22978505982835 29.341784562697214 28.379283440699847 22.049146936774587 39 20.454874992232323 28.48354288791764 28.902652056534855 22.158930063315175 40 20.900221637632825 27.955341052675188 29.404616414994027 21.73982089469796 41 19.21480898426442 28.905413896196258 28.772155132533783 23.107621987005544 42 20.601942954201792 27.709537322810725 29.43499665126941 22.25352307171807 43 20.996886025781773 27.3014755128391 29.088385773763903 22.61325268761522 44 20.165572287700837 28.338546305694223 28.13071787117399 23.36516353543095 45 19.95705339326525 28.638205908955953 27.79239251265268 23.612348185126113 46 21.17847698351872 29.127741988938833 27.310451491738647 22.383329535803796 47 20.114478253964965 27.816558609689913 29.745013153261386 22.323949983083732 48 20.456946371978376 27.464424052861613 29.677348081557124 22.40128149360289 49 20.369948422644324 26.15531205335874 30.34571327961555 23.129026244381382 50 19.37568614454088 27.319427470638193 29.829939722849392 23.474946661971536 51 19.490302490488915 26.869247605830243 28.539470141060963 25.10097976261988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 157.5 2 228.0 3 596.0 4 964.0 5 671.5 6 379.0 7 340.0 8 301.0 9 304.5 10 308.0 11 340.0 12 372.0 13 354.5 14 337.0 15 348.0 16 359.0 17 340.0 18 321.0 19 389.5 20 458.0 21 603.5 22 749.0 23 837.5 24 926.0 25 1160.5 26 1681.0 27 1967.0 28 2361.5 29 2756.0 30 3202.0 31 3648.0 32 4392.5 33 5137.0 34 5567.0 35 5997.0 36 6242.5 37 6488.0 38 7097.0 39 7706.0 40 8347.0 41 8988.0 42 9573.0 43 10158.0 44 10685.0 45 11212.0 46 11954.0 47 12696.0 48 12640.0 49 12584.0 50 11959.5 51 11335.0 52 10028.0 53 8721.0 54 7581.0 55 6441.0 56 5884.5 57 5328.0 58 5000.5 59 4673.0 60 4199.0 61 3725.0 62 3204.5 63 2684.0 64 2248.0 65 1812.0 66 1475.5 67 1139.0 68 1003.5 69 868.0 70 761.0 71 654.0 72 544.5 73 435.0 74 303.5 75 156.0 76 140.0 77 113.0 78 86.0 79 66.5 80 47.0 81 36.0 82 25.0 83 21.0 84 17.0 85 10.5 86 4.0 87 3.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 144831.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.382383605719774 #Duplication Level Percentage of deduplicated Percentage of total 1 69.97385383313993 34.5547569235868 2 10.698955551516338 10.5667985445105 3 5.701821842535758 8.447086604387183 4 3.7918932900826334 7.490109161712617 5 2.567078200808154 6.33842202290946 6 1.9518742746885531 5.78329225096837 7 1.382810643027922 4.78005399396538 8 0.9955118077208093 3.932859677831403 9 0.7885795780260343 3.5047745303146423 >10 2.1308426895597097 13.682844142483308 >50 0.013981907411809118 0.452941704469347 >100 0.002796381482361824 0.46606044286098974 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.334873058944563 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 190 0.13118738391642673 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05868909280471722 0.0 2 0.0 0.0 0.0 0.30449282266917993 0.0 3 0.0 0.0 0.0 0.4481084850618997 0.0 4 0.0 0.0 0.0 0.6614605989049306 0.0 5 0.0 0.0 0.0 1.203471632454378 0.0 6 0.0 0.0 0.0 1.5411065310603393 0.0 7 0.0 0.0 0.0 1.878050969750951 0.0 8 0.0 0.0 0.0 2.653437454688568 0.0 9 0.0 0.0 0.0 2.982786834310334 0.0 10 0.0 0.0 0.0 3.7844107960312363 0.0 11 0.0 0.0 0.0 4.731721799890908 0.0 12 0.0 0.0 0.0 5.484323107621987 0.0 13 0.0 0.0 0.0 5.799863288936761 0.0 14 0.0 0.0 0.0 5.934502972429936 0.0 15 0.0 0.0 0.0 6.14164094703482 0.0 16 0.0 0.0 0.0 6.569035634636231 0.0 17 0.0 0.0 0.0 7.137284144968964 0.0 18 0.0 0.0 0.0 7.786316465397602 0.0 19 0.0 0.0 0.0 8.143284241633353 0.0 20 0.0 0.0 0.0 8.567226629658014 0.0 21 0.0 0.0 0.0 9.06090546913299 0.0 22 0.0 0.0 0.0 9.596011903528941 0.0 23 0.0 0.0 0.0 10.101428561564859 0.0 24 0.0 0.0 0.0 10.503276232298333 0.0 25 0.0 0.0 0.0 10.858863088703385 0.0 26 0.0 0.0 0.0 11.197188447224697 0.0 27 0.0 0.0 0.0 11.564513122190691 0.0 28 0.0 0.0 0.0 11.934599636818085 0.0 29 0.0 0.0 0.0 12.321947649329218 0.0 30 0.0 0.0 0.0 12.753485096422727 0.0 31 0.0 0.0 0.0 13.16154690639435 0.0 32 0.0 0.0 0.0 13.529562041275694 0.0 33 0.0 0.0 0.0 13.883077517934696 0.0 34 0.0 0.0 0.0 14.266282770953731 0.0 35 0.0 0.0 0.0 14.660535382618361 0.0 36 0.0 0.0 0.0 15.053407074452293 0.0 37 0.0 0.0 0.0 15.45249290552437 0.0 38 0.0 0.0 0.0 15.859173795665292 0.0 39 0.0 0.0 0.0 16.297615841912297 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATCC 25 3.8738803E-5 45.0 8 GGGTCGT 20 7.012857E-4 45.0 7 AGGACCG 20 7.012857E-4 45.0 7 ATGTCTC 20 7.012857E-4 45.0 7 AGGCGAT 35 1.2021155E-7 45.0 7 GTCGTTT 20 7.012857E-4 45.0 9 TGCCTTA 20 7.012857E-4 45.0 12 TATAGCG 20 7.012857E-4 45.0 1 CGGTCTA 25 3.8738803E-5 45.0 31 CAGTCAG 20 7.012857E-4 45.0 33 ATTGCGG 20 7.012857E-4 45.0 2 AGCTACG 35 1.2021155E-7 45.0 9 CTAGGAC 20 7.012857E-4 45.0 5 TCTGGTA 20 7.012857E-4 45.0 14 TTGGGTA 20 7.012857E-4 45.0 5 TTGGGCG 35 1.2021155E-7 45.0 5 ATAGCGG 30 2.1523356E-6 44.999996 2 GACCTAT 30 2.1523356E-6 44.999996 33 CGGGAAT 50 1.0677468E-9 40.5 6 TAGGGAC 135 0.0 40.0 5 >>END_MODULE