##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258830
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.097500289765485	33.0	31.0	34.0	30.0	34.0
2	32.236904531932154	33.0	31.0	34.0	30.0	34.0
3	32.086377158752846	33.0	31.0	34.0	30.0	34.0
4	35.830545145462274	37.0	35.0	37.0	35.0	37.0
5	35.91090290924545	37.0	35.0	37.0	35.0	37.0
6	35.89160839160839	37.0	35.0	37.0	35.0	37.0
7	26.788463470231427	36.0	10.0	37.0	0.0	37.0
8	31.18457288567786	37.0	19.0	37.0	17.0	37.0
9	36.04909399992273	39.0	32.0	39.0	32.0	39.0
10	36.78117297067573	38.0	35.0	39.0	33.0	39.0
11	37.20627438859483	39.0	37.0	39.0	34.0	39.0
12	37.26689719120658	39.0	37.0	39.0	34.0	39.0
13	37.2475292663138	39.0	37.0	39.0	34.0	39.0
14	38.410134064830196	40.0	38.0	41.0	34.0	41.0
15	38.49932388053935	40.0	38.0	41.0	34.0	41.0
16	38.42889927751806	40.0	38.0	41.0	34.0	41.0
17	38.35499362515937	40.0	38.0	41.0	34.0	41.0
18	38.240779662326624	40.0	37.0	41.0	34.0	41.0
19	38.126036394544684	40.0	37.0	41.0	34.0	41.0
20	38.194046285206504	40.0	37.0	41.0	34.0	41.0
21	38.125661631186496	40.0	37.0	41.0	34.0	41.0
22	38.15419773596569	40.0	37.0	41.0	34.0	41.0
23	38.0959896457134	40.0	37.0	41.0	34.0	41.0
24	38.04697291658618	40.0	37.0	41.0	34.0	41.0
25	37.94866901054746	40.0	37.0	41.0	33.0	41.0
26	37.829181315921645	40.0	36.0	41.0	33.0	41.0
27	37.72286829192907	40.0	36.0	41.0	33.0	41.0
28	37.71783796314183	40.0	36.0	41.0	33.0	41.0
29	37.68529923115558	40.0	36.0	41.0	33.0	41.0
30	37.59361743229147	40.0	36.0	41.0	33.0	41.0
31	37.470482556117915	40.0	36.0	41.0	33.0	41.0
32	37.345582042267125	40.0	36.0	41.0	32.0	41.0
33	37.24154077966233	40.0	36.0	41.0	32.0	41.0
34	37.079121431055135	40.0	36.0	41.0	31.0	41.0
35	36.94781130471738	40.0	35.0	41.0	31.0	41.0
36	36.81211992427462	39.0	35.0	41.0	31.0	41.0
37	36.759266700150675	39.0	35.0	41.0	31.0	41.0
38	36.621388556195186	39.0	35.0	41.0	30.0	41.0
39	36.525391183402235	39.0	35.0	41.0	30.0	41.0
40	36.372186377158755	39.0	35.0	41.0	30.0	41.0
41	36.30383263145694	39.0	35.0	41.0	30.0	41.0
42	36.234424139396516	39.0	35.0	41.0	30.0	41.0
43	36.2066993779701	39.0	35.0	40.0	30.0	41.0
44	36.01283081559325	39.0	35.0	40.0	29.0	41.0
45	35.89141907815941	38.0	35.0	40.0	28.0	41.0
46	35.78368813506935	38.0	35.0	40.0	28.0	41.0
47	35.63640999884094	38.0	35.0	40.0	27.0	41.0
48	35.57085345593633	38.0	35.0	40.0	27.0	41.0
49	35.41517598423676	38.0	34.0	40.0	27.0	41.0
50	35.23957423791678	38.0	34.0	40.0	26.0	41.0
51	33.20486806011668	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	28.0
10	24.0
11	13.0
12	11.0
13	4.0
14	9.0
15	14.0
16	25.0
17	41.0
18	95.0
19	190.0
20	292.0
21	447.0
22	643.0
23	942.0
24	1392.0
25	1869.0
26	2362.0
27	2860.0
28	3255.0
29	3777.0
30	4639.0
31	5724.0
32	7746.0
33	10461.0
34	16107.0
35	20700.0
36	24555.0
37	39929.0
38	52021.0
39	58637.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30212881041611	21.17258432175559	26.313023992582004	12.212262875246301
2	31.389328903141056	24.36386817602287	27.179229610168836	17.067573310667232
3	31.49248541513735	24.915581655913147	27.349611714252596	16.242321214696904
4	27.73132944403663	26.177027392497003	27.139435150484875	18.952208012981494
5	25.96028281111154	30.598462311169495	25.083645636131823	18.35760924158714
6	26.468338291542707	35.01371556620175	24.320596530541284	14.197349611714252
7	62.692887223273964	27.936869760074178	5.995827377042846	3.3744156396090097
8	85.75628791098404	4.805084418344087	6.356681992041108	3.0819456786307615
9	78.42908472742727	8.69605532588958	8.20924931422169	4.665610632461461
10	35.42247807441178	36.92230421512189	14.643974809720667	13.011242900745662
11	32.17942278715759	25.653131398987757	24.52072789089364	17.646717922961017
12	29.266700150678048	24.03971718888846	26.658424448479696	20.035158211953792
13	26.435498203454006	24.692655410887454	28.975002897654832	19.89684348800371
14	21.992041108063205	26.88212340146042	29.876753081172968	21.249082409303405
15	21.02963335007534	25.656994938762896	32.0951976200595	21.218174091102266
16	24.834833674612682	26.39647645172507	29.494262643433917	19.274427230228337
17	25.563110922226944	25.40238766758104	27.666035621836727	21.36846578835529
18	25.55229301085655	24.354982034540047	29.343198238225867	20.749526716377545
19	25.259050341923274	26.296797125526407	27.231773751110765	21.212378781439554
20	26.868987366224932	26.692809952478463	27.24490978634625	19.193292894950353
21	26.181663640227175	26.13800564076807	28.215817331839432	19.464513387165322
22	25.570065293822196	24.00571803886721	28.015299617509566	22.408917049801026
23	22.985743538229723	26.73917242978017	29.093613568751692	21.18147046323842
24	22.943244600703164	25.335162075493567	29.508171386624426	22.213421937178843
25	22.83081559324653	27.112776726036397	28.064366572653864	21.992041108063205
26	22.69172816134142	27.13170807093459	27.587605764401346	22.588958003322645
27	21.834408685237413	26.781285013329214	29.301085654676818	22.08322064675656
28	20.217130935362977	27.608855233164626	29.13572615230074	23.038287679171656
29	22.393076536722944	27.144071398215047	28.85523316462543	21.607618900436577
30	23.354325232778272	25.57470154155237	29.16817988641193	21.90279333925743
31	23.814472819997683	26.55990418421358	27.883166557199708	21.742456438589038
32	23.90835683653363	27.40292856314956	27.052891859521694	21.635822740795117
33	23.659931228992004	26.406135301162927	27.217478653942745	22.716454815902328
34	22.481551597573695	27.52617548197659	28.669010547463586	21.32326237298613
35	21.455395433295983	27.461268013754204	28.276861260286672	22.80647529266314
36	22.49777846462929	28.20731754433412	27.160298265270644	22.134605725765947
37	22.372986129892208	28.72194104238303	26.99300699300699	21.91206583471777
38	22.417030483328826	28.7999845458409	26.22995788741645	22.553027083413824
39	22.712591276127185	27.15759378742804	26.618243634818224	23.51157130162655
40	24.26225707993664	25.307730943090057	27.555152030290152	22.874859946683152
41	21.50291697253023	27.12127651354171	27.05173279758915	24.32407371633891
42	21.975041533052583	26.5796082370668	28.378472356372907	23.066877873507707
43	21.978905072827725	26.46756558358768	27.850326469110996	23.703202874473593
44	22.11181084109261	26.22957153343894	28.087161457327202	23.57145616814125
45	22.173627477494882	26.26782057721284	27.356179731870338	24.20237221342194
46	22.439439014024646	26.95591701116563	26.800602712204924	23.804041262604798
47	20.844569794846038	27.114708495923967	28.972684773789748	23.06803693544025
48	21.713866244252987	26.560676892168605	28.151296217594563	23.57416064598385
49	22.112197195070124	26.003168102615614	28.609898388903915	23.274736313410347
50	21.179152339373335	26.411544256848124	28.624193486071942	23.785109917706603
51	21.22126492292238	26.197117799327746	27.69848935594792	24.883127921801957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	271.0
2	445.0
3	1176.5
4	1908.0
5	1293.5
6	679.0
7	600.5
8	522.0
9	505.0
10	488.0
11	498.0
12	508.0
13	539.0
14	570.0
15	583.0
16	596.0
17	598.5
18	601.0
19	619.5
20	638.0
21	702.5
22	767.0
23	1032.0
24	1297.0
25	1612.0
26	2276.5
27	2626.0
28	3196.0
29	3766.0
30	4376.0
31	4986.0
32	5584.5
33	6183.0
34	6907.0
35	7631.0
36	8632.5
37	9634.0
38	10422.5
39	11211.0
40	12269.0
41	13327.0
42	14312.5
43	15298.0
44	16465.5
45	17633.0
46	19280.0
47	20927.0
48	20871.5
49	20816.0
50	20469.5
51	20123.0
52	19138.5
53	18154.0
54	16697.5
55	15241.0
56	14203.5
57	13166.0
58	12424.0
59	11682.0
60	10928.5
61	10175.0
62	9340.5
63	8506.0
64	7165.5
65	5825.0
66	4894.0
67	3963.0
68	3236.5
69	2510.0
70	2078.5
71	1647.0
72	1379.5
73	1112.0
74	914.0
75	581.5
76	447.0
77	325.0
78	203.0
79	160.0
80	117.0
81	99.5
82	82.0
83	57.5
84	33.0
85	28.0
86	23.0
87	18.0
88	13.0
89	9.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.508167146164084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.731085707394975	25.411213050955006
2	11.698688018697721	9.47784819304469
3	6.693454772759136	8.134187541606504
4	4.526476806749473	7.33437116284171
5	3.242122855066924	6.566622726072485
6	2.4772595650414573	6.020954671507986
7	1.9139962283802756	5.4272735395449105
8	1.4843411403598146	4.810235121249854
9	1.2231605383887598	4.4593192382079545
>10	3.987483209559678	21.051636174053588
>50	0.01295932040103189	0.36585556547427595
>100	0.007974966400635008	0.5637244695387299
>500	9.96870800079376E-4	0.37675854590231733
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.3604682610207472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.052930494919445195	0.0
2	0.0	0.0	0.0	0.2573117490244562	0.0
3	0.0	0.0	0.0	0.3531275354479774	0.0
4	0.0	0.0	0.0	0.4752153923424642	0.0
5	0.0	0.0	0.0	0.9098636170459374	0.0
6	0.0	0.0	0.0	1.1609937024301664	0.0
7	0.0	0.0	0.0	1.4152146196345092	0.0
8	0.0	0.0	0.0	2.0287447359270563	0.0
9	0.0	0.0	0.0	2.2783294054012284	0.0
10	0.0	0.0	0.0	2.921608777962369	0.0
11	0.0	0.0	0.0	3.4910945408182976	0.0
12	0.0	0.0	0.0	4.041262604798517	0.0
13	0.0	0.0	0.0	4.302437893598115	0.0
14	0.0	0.0	0.0	4.3901402464938375	0.0
15	0.0	0.0	0.0	4.594135146621334	0.0
16	0.0	0.0	0.0	4.883514275779469	0.0
17	0.0	0.0	0.0	5.256732218058185	0.0
18	0.0	0.0	0.0	5.645404319437469	0.0
19	0.0	0.0	0.0	5.872194104238303	0.0
20	3.863539775141985E-4	0.0	0.0	6.12680137542016	0.0
21	3.863539775141985E-4	0.0	0.0	6.436657265386547	0.0
22	3.863539775141985E-4	0.0	0.0	6.781671367306727	0.0
23	3.863539775141985E-4	0.0	0.0	7.101572460688483	0.0
24	3.863539775141985E-4	0.0	0.0	7.3708611830158794	0.0
25	3.863539775141985E-4	0.0	0.0	7.6212185604450795	0.0
26	3.863539775141985E-4	0.0	0.0	7.851099177066028	0.0
27	3.863539775141985E-4	0.0	0.0	8.07943437777692	0.0
28	3.863539775141985E-4	0.0	0.0	8.314723950083065	0.0
29	3.863539775141985E-4	0.0	0.0	8.559672371827068	0.0
30	3.863539775141985E-4	0.0	0.0	8.88189158907391	0.0
31	3.863539775141985E-4	0.0	0.0	9.14693041764865	0.0
32	3.863539775141985E-4	0.0	0.0	9.425105281458872	0.0
33	3.863539775141985E-4	0.0	0.0	9.701734729359039	0.0
34	7.72707955028397E-4	0.0	0.0	9.966387203956264	0.0
35	7.72707955028397E-4	0.0	0.0	10.272765908125024	0.0
36	7.72707955028397E-4	0.0	0.0	10.548236294092648	0.0
37	7.72707955028397E-4	0.0	0.0	10.847274272688637	0.0
38	7.72707955028397E-4	0.0	0.0	11.145153189352085	0.0
39	7.72707955028397E-4	0.0	0.0	11.543097786191709	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.023149E-4	45.0	21
CTCGTGC	20	7.023149E-4	45.0	45
TTAATCG	20	7.023149E-4	45.0	20
GACACGA	20	7.023149E-4	45.0	25
CTACGAA	25	3.8824037E-5	45.0	11
GTCAAGC	25	3.8824037E-5	45.0	16
GGACGTA	25	3.8824037E-5	45.0	8
TCGTAAA	25	3.8824037E-5	45.0	1
ACGTCGA	20	7.023149E-4	45.0	30
CGTAAAG	25	3.8824037E-5	45.0	2
CGTCGAC	20	7.023149E-4	45.0	31
CGATGCA	20	7.023149E-4	45.0	10
TACGGGA	100	0.0	40.5	4
CGTTTTT	290	0.0	40.344826	1
TTGACGG	45	1.9183062E-8	40.000004	2
ACGACGC	40	3.444402E-7	39.375	14
GGCCGGT	105	0.0	38.57143	9
CGGGATC	70	0.0	38.571426	6
ACGGGAT	95	0.0	37.894737	5
TACGAAT	30	1.1374791E-4	37.500004	12
>>END_MODULE