##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104201
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.924213779138395	33.0	31.0	34.0	30.0	34.0
2	32.02822429727162	33.0	31.0	34.0	30.0	34.0
3	31.893964549284554	33.0	31.0	34.0	30.0	34.0
4	35.667277665281524	37.0	35.0	37.0	33.0	37.0
5	35.77840903638161	37.0	35.0	37.0	35.0	37.0
6	35.74981046247157	37.0	35.0	37.0	35.0	37.0
7	26.920010364583835	35.0	11.0	37.0	0.0	37.0
8	31.197157416915385	35.0	19.0	37.0	17.0	37.0
9	35.9605378067389	39.0	32.0	39.0	32.0	39.0
10	36.60366983042389	38.0	35.0	39.0	32.0	39.0
11	36.97329200295582	39.0	37.0	39.0	33.0	39.0
12	36.97548008176505	39.0	37.0	39.0	33.0	39.0
13	36.881613420216695	39.0	37.0	39.0	33.0	39.0
14	38.031477624974805	40.0	37.0	41.0	33.0	41.0
15	38.09616030556329	40.0	37.0	41.0	33.0	41.0
16	38.07392443450639	40.0	37.0	41.0	33.0	41.0
17	37.97575839003464	40.0	37.0	41.0	33.0	41.0
18	37.87827372098157	40.0	36.0	41.0	33.0	41.0
19	37.734071649984166	40.0	36.0	41.0	33.0	41.0
20	37.82626846191495	40.0	36.0	41.0	33.0	41.0
21	37.76547250026391	40.0	36.0	41.0	33.0	41.0
22	37.78654715405802	39.0	36.0	41.0	33.0	41.0
23	37.76014625579409	39.0	36.0	41.0	33.0	41.0
24	37.69521405744666	39.0	36.0	41.0	33.0	41.0
25	37.58582931065921	39.0	36.0	41.0	33.0	41.0
26	37.45735645531233	39.0	35.0	41.0	33.0	41.0
27	37.378124970009885	39.0	35.0	41.0	33.0	41.0
28	37.335620579457014	39.0	35.0	41.0	32.0	41.0
29	37.31662843926642	39.0	35.0	41.0	32.0	41.0
30	37.24654273951305	39.0	35.0	41.0	32.0	41.0
31	37.10901047014904	39.0	35.0	41.0	32.0	41.0
32	36.94565311273404	39.0	35.0	41.0	31.0	41.0
33	36.88797612306983	39.0	35.0	41.0	31.0	41.0
34	36.699206341589814	39.0	35.0	41.0	31.0	41.0
35	36.53352654964924	39.0	35.0	41.0	30.0	41.0
36	36.387625838523626	39.0	35.0	41.0	30.0	41.0
37	36.320985403211104	39.0	35.0	40.0	30.0	41.0
38	36.15555512902947	39.0	35.0	40.0	30.0	41.0
39	36.12183184422415	39.0	35.0	40.0	30.0	41.0
40	35.88505868465754	39.0	35.0	40.0	29.0	41.0
41	35.751326762699016	38.0	35.0	40.0	28.0	41.0
42	35.705156380457	38.0	35.0	40.0	27.0	41.0
43	35.693716950892984	38.0	35.0	40.0	27.0	41.0
44	35.515407721614956	38.0	34.0	40.0	27.0	41.0
45	35.3876930163818	38.0	34.0	40.0	27.0	41.0
46	35.22746422779052	38.0	34.0	40.0	26.0	41.0
47	35.005854070498366	38.0	34.0	40.0	25.0	41.0
48	34.905317607316626	38.0	34.0	40.0	24.0	41.0
49	34.752056122302086	38.0	34.0	40.0	24.0	41.0
50	34.55530177253577	37.0	34.0	40.0	24.0	41.0
51	32.38123434515983	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	2.0
11	5.0
12	4.0
13	0.0
14	2.0
15	6.0
16	13.0
17	25.0
18	35.0
19	76.0
20	120.0
21	177.0
22	289.0
23	411.0
24	715.0
25	904.0
26	1130.0
27	1402.0
28	1603.0
29	1867.0
30	2158.0
31	2765.0
32	3671.0
33	5057.0
34	7557.0
35	9382.0
36	9872.0
37	15297.0
38	19337.0
39	20299.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77982936824023	21.371196053780675	26.42776940720338	10.421205170775712
2	30.46515868369785	25.776144182877324	26.675367798773525	17.0833293346513
3	30.661893839790405	25.82316868360188	27.570752679916698	15.94418479669101
4	26.860586750607002	26.089001065248894	28.749244249095497	18.301167935048607
5	26.10339632057274	30.03426070767075	25.685933916181224	18.17640905557528
6	24.979606721624553	35.81059682728573	25.562134720396156	13.647661730693564
7	62.007082465619334	27.22238750107964	7.258087734282781	3.512442299018244
8	83.43010143856586	5.593996218846269	7.36077388892621	3.615128453661673
9	77.6182570224854	7.02968301647777	8.916421147589755	6.435638813447088
10	38.44396886786115	31.616779109605474	16.038233798140134	13.90101822439324
11	31.914281052964945	25.010316599648757	25.519908638112877	17.555493709273424
12	28.614888532739606	24.08902025892266	28.30683006880932	18.989261139528413
13	24.48536962217253	27.65232579341849	30.056333432500647	17.80597115190833
14	21.068895691979925	29.946929492039427	28.62256600224566	20.361608813734993
15	19.60921680214201	26.78093300448172	34.94784119154327	18.662009001832995
16	23.310716787746756	26.00838763543536	30.88166140440111	19.79923417241677
17	23.082312069941747	25.047744263490753	28.55922687882074	23.310716787746756
18	24.945058108847324	24.9412193740943	30.69164403412635	19.422078482932026
19	23.97865663477318	27.418162973483938	27.312597767775742	21.29058262396714
20	24.6926612988359	28.029481482903236	28.119691749599333	19.15816546866153
21	24.0573506972102	28.629283788063454	28.599533593727504	18.71383192099884
22	22.62838168539649	25.590925231043848	28.03140085027975	23.749292233279913
23	20.315543996698686	29.573612537307703	28.739647412212932	21.371196053780675
24	21.925893225592844	26.17441291350371	30.094720780030904	21.804973080872543
25	21.27042926651376	29.287626798207313	28.066909146745232	21.3750347885337
26	20.29155190449228	28.819301158338213	28.22333758793102	22.66580934923849
27	20.945096496194854	28.85193040373893	28.278039558161627	21.924933541904586
28	18.116908666903388	28.62544505331043	31.139816316542067	22.117829963244116
29	20.03147762497481	27.085152733658983	29.752113703323385	23.131255938042823
30	21.565052158808456	27.91527912400073	29.5208299344536	20.99883878273721
31	22.762737401752382	28.11009491271677	26.675367798773525	22.451799886757325
32	23.304958685617223	28.16671625032389	26.374986804349287	22.1533382597096
33	22.375984875385075	27.001660252780685	26.280937802900162	24.341417068934078
34	20.732046717401946	28.063070411992207	28.225256955307533	22.979625915298318
35	19.851057091582614	29.156150132916192	27.732939223232023	23.25985355226917
36	21.573689312002763	29.985316839569677	26.570762276753584	21.870231571673976
37	20.34625387472289	29.98723620694619	27.648487058665467	22.018022859665454
38	19.455667412020997	31.10814675482961	26.217598679475245	23.21858715367415
39	22.147580157580062	26.799166994558593	27.13697565282483	23.916277195036518
40	22.972908129480523	26.423930672450364	27.926795328259807	22.676365869809313
41	20.14951871863034	27.164806479784264	27.916238807688988	24.769435993896412
42	21.33856680837996	26.799166994558593	28.19358739359507	23.668678803466378
43	21.086169998368536	26.6341014001785	28.218539169489738	24.061189431963225
44	20.781950269191274	26.762699014404852	28.08418345313385	24.371167263270028
45	20.782909952879532	27.55155900615157	26.25118760856422	25.414343432404678
46	22.348154048425638	27.94406963464842	26.890336944942945	22.817439371982996
47	20.328979568334276	27.230064970585694	30.03809944242378	22.40285601865625
48	21.10344430475715	27.423921075613478	28.350975518469113	23.121659101160258
49	21.315534399861804	25.625473843821077	30.051535014059365	23.00745674225775
50	19.77044366176908	27.227185919520924	29.30394142090767	23.698428997802324
51	20.478690223702266	26.118751259584837	26.991103732209865	26.41145478450303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	115.5
2	184.0
3	409.0
4	634.0
5	458.0
6	282.0
7	264.0
8	246.0
9	242.0
10	238.0
11	237.0
12	236.0
13	257.0
14	278.0
15	260.5
16	243.0
17	260.0
18	277.0
19	329.0
20	381.0
21	409.0
22	437.0
23	526.0
24	615.0
25	717.0
26	945.5
27	1072.0
28	1215.0
29	1358.0
30	1646.5
31	1935.0
32	2232.5
33	2530.0
34	2965.0
35	3400.0
36	3620.0
37	3840.0
38	4274.5
39	4709.0
40	5095.5
41	5482.0
42	6130.0
43	6778.0
44	7379.0
45	7980.0
46	9452.0
47	10924.0
48	10200.5
49	9477.0
50	9347.0
51	9217.0
52	8367.0
53	7517.0
54	6508.0
55	5499.0
56	4848.5
57	4198.0
58	3910.0
59	3622.0
60	3287.0
61	2952.0
62	2565.0
63	2178.0
64	1899.0
65	1620.0
66	1374.0
67	1128.0
68	901.0
69	674.0
70	584.5
71	495.0
72	393.5
73	292.0
74	230.5
75	123.5
76	78.0
77	72.0
78	66.0
79	53.5
80	41.0
81	38.5
82	36.0
83	24.0
84	12.0
85	7.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	104201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.29124480571203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99427396858023	38.692526943119546
2	9.552562032007048	9.990307194748612
3	4.88731463808545	7.666912985479986
4	3.097929819409778	6.479784263106881
5	2.220672441638526	5.806086313950922
6	1.5764939069152841	4.946209729273232
7	1.2149464102187637	4.447174211379929
8	0.8974453090588754	3.7542825884588438
9	0.6588606665687857	3.100737996756269
>10	1.8701365438261637	12.722526655214441
>50	0.01651739832623697	0.5662133760712469
>100	0.012846865364850977	1.8272377424400916
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	456	0.4376157618448959	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	394	0.3781153731730022	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	342	0.32821182138367194	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	306	0.2936632086064433	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	172	0.16506559438009233	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	126	0.12092014472030019	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	108	0.10364583833168588	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0815731135017898	0.0
2	0.0	0.0	0.0	0.5431809675530945	0.0
3	0.0	0.0	0.0	0.7312789704513393	0.0
4	0.0	0.0	0.0	1.0201437606165007	0.0
5	0.0	0.0	0.0	1.9855855510023896	0.0
6	0.0	0.0	0.0	2.3838542816287753	0.0
7	0.0	0.0	0.0	2.859857391003925	0.0
8	0.0	0.0	0.0	3.7974683544303796	0.0
9	0.0	0.0	0.0	4.148712584332204	0.0
10	0.0	0.0	0.0	5.548891085498219	0.0
11	0.0	0.0	0.0	6.50857477375457	0.0
12	0.0	0.0	0.0	7.73409084365793	0.0
13	0.0	0.0	0.0	8.13907736010211	0.0
14	0.0	0.0	0.0	8.285908964405332	0.0
15	0.0	0.0	0.0	8.603564265218184	0.0
16	0.0	0.0	0.0	9.14098713064174	0.0
17	0.0	0.0	0.0	9.782055834396983	0.0
18	0.0	0.0	0.0	10.503737967965758	0.0
19	0.0	0.0	0.0	10.980700761029166	0.0
20	0.0	0.0	0.0	11.39720348173242	0.0
21	0.0	0.0	0.0	11.884722795366647	0.0
22	0.0	0.0	0.0	12.426944079231486	0.0
23	0.0	0.0	0.0	13.067053099298471	0.0
24	0.0	0.0	0.0	13.488354238443009	0.0
25	0.0	0.0	0.0	13.925970000287904	0.0
26	0.0	0.0	0.0	14.317520945096495	0.0
27	0.0	0.0	0.0	14.657248970739245	0.0
28	0.0	0.0	0.0	15.074711375130757	0.0
29	0.0	0.0	0.0	15.497931881651807	0.0
30	0.0	0.0	0.0	16.013282022245466	0.0
31	0.0	0.0	0.0	16.424986324507444	0.0
32	0.0	0.0	0.0	16.82517442251034	0.0
33	0.0	0.0	0.0	17.263749868043494	0.0
34	0.0	0.0	0.0	17.65434112916383	0.0
35	0.0	0.0	0.0	18.105392462644314	0.0
36	0.0	0.0	0.0	18.511338662776748	0.0
37	0.0	0.0	0.0	18.919204230285697	0.0
38	0.0	0.0	0.0	19.379852400648748	0.0
39	0.0	0.0	0.0	19.920154317137072	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTC	20	7.0037605E-4	45.000004	12
GTACAAG	20	7.0037605E-4	45.000004	1
GAATCGG	20	7.0037605E-4	45.000004	2
GCGTTAG	20	7.0037605E-4	45.000004	1
CAGGGCG	20	7.0037605E-4	45.000004	5
CGTTTTA	20	7.0037605E-4	45.000004	1
CGATATG	30	2.146484E-6	45.000004	10
GAGGGCG	30	2.146484E-6	45.000004	5
AATAAGG	20	7.0037605E-4	45.000004	3
ACGAAGA	30	2.146484E-6	45.000004	33
CTATCTC	25	3.8663526E-5	45.0	5
GCGATAT	35	1.1977681E-7	45.0	9
CTTCTGC	50	2.1827873E-11	45.0	45
GCCACCG	25	3.8663526E-5	45.0	9
CTAGGAC	25	3.8663526E-5	45.0	5
ACGGCTG	60	3.6379788E-12	41.250004	8
GGCGATA	55	6.002665E-11	40.909092	8
TACGGCT	50	1.0622898E-9	40.5	7
ATGGGAC	80	0.0	39.375004	5
GAATGAC	40	3.4180266E-7	39.375004	1
>>END_MODULE