##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132157
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7345051718789	31.0	31.0	34.0	30.0	34.0
2	31.855959200042374	31.0	31.0	34.0	30.0	34.0
3	31.75485218338794	31.0	31.0	34.0	30.0	34.0
4	35.54422391549445	37.0	35.0	37.0	33.0	37.0
5	35.64424888579492	37.0	35.0	37.0	35.0	37.0
6	35.63566818254046	37.0	35.0	37.0	33.0	37.0
7	27.297116308632916	35.0	25.0	37.0	0.0	37.0
8	31.386055978873614	35.0	27.0	37.0	17.0	37.0
9	35.97076204816998	39.0	32.0	39.0	32.0	39.0
10	36.47341419675083	37.0	35.0	39.0	32.0	39.0
11	36.83577865720318	39.0	37.0	39.0	33.0	39.0
12	36.997896441353845	39.0	37.0	39.0	33.0	39.0
13	36.9484703799269	39.0	37.0	39.0	33.0	39.0
14	38.05864237232988	40.0	37.0	41.0	33.0	41.0
15	38.16462238095598	40.0	37.0	41.0	33.0	41.0
16	38.07206580052513	40.0	37.0	41.0	33.0	41.0
17	38.007521357173665	40.0	37.0	41.0	33.0	41.0
18	37.92726075803779	40.0	37.0	41.0	33.0	41.0
19	37.8139031606347	39.0	37.0	41.0	33.0	41.0
20	37.87736555763221	39.0	37.0	41.0	33.0	41.0
21	37.830323024887065	39.0	36.0	41.0	33.0	41.0
22	37.879272380577646	39.0	37.0	41.0	33.0	41.0
23	37.84736336327247	39.0	36.0	41.0	33.0	41.0
24	37.80909826948251	39.0	36.0	41.0	33.0	41.0
25	37.71202433469283	39.0	36.0	41.0	33.0	41.0
26	37.59106214578115	39.0	36.0	41.0	33.0	41.0
27	37.415467966131196	39.0	36.0	41.0	32.0	41.0
28	37.422194813744255	39.0	36.0	41.0	32.0	41.0
29	37.295353254084155	39.0	36.0	41.0	32.0	41.0
30	37.13086707476713	39.0	36.0	41.0	31.0	41.0
31	37.02812563844518	39.0	35.0	41.0	31.0	41.0
32	36.70168814364733	39.0	35.0	41.0	30.0	41.0
33	36.689377028836915	39.0	35.0	40.0	30.0	41.0
34	36.56221766535257	39.0	35.0	40.0	30.0	41.0
35	36.29507328404852	39.0	35.0	40.0	30.0	41.0
36	36.184076515054066	39.0	35.0	40.0	30.0	41.0
37	35.92410542006856	39.0	35.0	40.0	28.0	41.0
38	35.85971231187148	38.0	35.0	40.0	28.0	41.0
39	35.810066814470666	39.0	35.0	40.0	27.0	41.0
40	35.67877600127122	38.0	35.0	40.0	27.0	41.0
41	35.72410088001392	38.0	35.0	40.0	27.0	41.0
42	35.601337802764895	38.0	35.0	40.0	26.0	41.0
43	35.50754784082569	38.0	35.0	40.0	26.0	41.0
44	35.22094175866583	38.0	34.0	40.0	25.0	41.0
45	35.08868240047822	38.0	34.0	40.0	24.0	41.0
46	34.84770386736987	38.0	34.0	40.0	23.0	41.0
47	34.68352035836165	38.0	33.0	40.0	23.0	41.0
48	34.56191499504377	38.0	33.0	40.0	23.0	41.0
49	34.35483553652096	38.0	33.0	40.0	22.0	41.0
50	34.050848611878294	38.0	33.0	40.0	20.0	41.0
51	31.49939087600354	35.0	28.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	9.0
11	4.0
12	3.0
13	1.0
14	3.0
15	4.0
16	5.0
17	7.0
18	35.0
19	71.0
20	158.0
21	228.0
22	403.0
23	606.0
24	878.0
25	1272.0
26	1521.0
27	1882.0
28	2253.0
29	2570.0
30	3239.0
31	3842.0
32	4798.0
33	6395.0
34	9048.0
35	11873.0
36	12927.0
37	19243.0
38	24485.0
39	24378.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.1588338113002	23.041533933124995	29.365830035488095	10.433802220086715
2	27.683739794335526	26.749245215917433	28.335237634026196	17.231777355720848
3	28.920904681553	26.286159643454376	29.601156200579616	15.191779474413009
4	25.98878606505898	25.185196395196623	30.654448875201467	18.17156866454293
5	24.398253592318227	28.936038196992968	27.832804921419218	18.83290328926958
6	24.58288248068585	34.5081985819896	26.341396974810266	14.567521962514283
7	61.99444599983354	25.870744644627226	7.684799140416324	4.450010215122922
8	81.69979645421733	4.532487874270754	8.311326679631046	5.456388991880869
9	76.06256195282883	7.02422119146167	10.013090490855573	6.900126364853924
10	34.135157426394365	36.51565940510151	17.00023456948932	12.348948599014808
11	23.998728784703044	26.398147657710147	30.355561945262078	19.247561612324734
12	23.208002602964655	23.30410042600846	34.21006832782221	19.27782864320467
13	20.752589722829665	26.166604871478622	35.58948826017539	17.491317145516316
14	17.01536808492929	30.679419175677413	31.204552161444344	21.100660577948954
15	15.383975120500617	27.072345770560773	38.81822377929281	18.725455329645797
16	17.247667546932817	27.844911733771195	33.368644869359926	21.538775849936062
17	17.700159658587893	27.468087199315967	30.554567673297665	24.277185468798475
18	18.619520721566015	26.44506155557405	34.61261983852539	20.32279788433454
19	18.820039801145608	28.58342728724169	30.66125895714945	21.935273954463252
20	19.831715308307544	29.410473906036	31.521599309911696	19.236211475744756
21	18.972131631317296	29.30983602836021	32.51511459854567	19.202917741776826
22	18.055797271427167	26.365610599514216	30.068781827674663	25.50981030138396
23	16.375220381818593	28.541810119781776	32.354699334882	22.72827016351763
24	18.933541166945375	25.863934562679237	32.08910613891054	23.11341813146485
25	16.624166710806087	29.923500079450953	30.595428164985584	22.856905044757372
26	17.88857192581551	29.034406047352768	30.33967175405011	22.737350272781615
27	18.726212005417796	30.110398995134574	29.821348850231168	21.342040149216462
28	16.03925633905128	27.708710094811472	33.353511353919956	22.898522212217287
29	19.831715308307544	24.452734247902118	32.67931324106934	23.036237202721004
30	19.550988596896115	29.10780359723662	30.587104731493604	20.75410307437366
31	22.227350802454655	28.19146923734649	26.98986811141294	22.591311848785914
32	21.415437699100313	29.049539562792738	28.438145539017984	21.09687719908896
33	19.637249634903938	26.983058029464956	29.941660297978917	23.43803203765219
34	19.82868860521955	25.120878954576753	30.066511800358665	24.983920639845035
35	17.72891333792383	28.109748253970658	30.149746135278495	24.011592272827016
36	21.398034156344348	26.35426046293424	29.682877183955448	22.56482819676597
37	18.277503272622713	28.625801130473604	31.840159809923048	21.25653578698064
38	17.71529317402786	28.45327905445795	30.6362886566735	23.195139114840682
39	18.594550421090066	24.782644884493443	32.521924680493655	24.100880013922836
40	19.19762101137284	25.586991230127804	31.849996594959023	23.365391163540334
41	17.350575451924605	27.172226972464568	27.648932708823597	27.82826486678723
42	19.639519662219936	26.53359262089787	30.08845539774662	23.738432319135576
43	21.149087827356855	25.261620648168464	29.504301701763815	24.084989822710867
44	20.163895972214867	25.07169502939685	30.089212073518617	24.675196924869663
45	19.277071967432676	25.331991494964324	29.67455375046346	25.71638278713954
46	21.924680493655273	26.306589889298333	29.520191892975777	22.248537724070612
47	17.140976263081033	25.294914382136398	32.925232866968834	24.63887648781374
48	18.586983663370084	25.061101568588874	30.833781033165096	25.518133734875942
49	19.634979607587944	22.296208297706517	34.170721187678296	23.898090907027246
50	18.786746067177678	22.542127923606014	32.90480262112487	25.76632338809144
51	18.04520381061919	22.64200912550981	30.067268476130664	29.24551858774034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	104.0
2	143.0
3	390.0
4	637.0
5	454.0
6	271.0
7	260.0
8	249.0
9	266.0
10	283.0
11	291.5
12	300.0
13	307.0
14	314.0
15	346.0
16	378.0
17	376.5
18	375.0
19	436.0
20	497.0
21	606.0
22	715.0
23	810.0
24	905.0
25	1057.5
26	1527.0
27	1844.0
28	2090.5
29	2337.0
30	2731.5
31	3126.0
32	3497.5
33	3869.0
34	4425.0
35	4981.0
36	5289.5
37	5598.0
38	6168.0
39	6738.0
40	7385.0
41	8032.0
42	8861.5
43	9691.0
44	10953.5
45	12216.0
46	14241.0
47	16266.0
48	16116.5
49	15967.0
50	14508.0
51	13049.0
52	10588.0
53	8127.0
54	6415.0
55	4703.0
56	3863.0
57	3023.0
58	2521.0
59	2019.0
60	1755.0
61	1491.0
62	1291.5
63	1092.0
64	917.0
65	742.0
66	570.0
67	398.0
68	317.5
69	237.0
70	173.5
71	110.0
72	92.0
73	74.0
74	55.5
75	27.5
76	18.0
77	14.5
78	11.0
79	8.5
80	6.0
81	7.5
82	9.0
83	5.0
84	1.0
85	2.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	132157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.50508864456669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48069002723793	44.555339482585104
2	9.904338329144572	11.390997071664763
3	4.198849954603471	7.243657165341223
4	2.377725436530389	5.469252480004843
5	1.625064147268971	4.672472892090468
6	1.1329394581365055	3.908987038144026
7	0.7868731660460281	3.1674447815855387
8	0.6381830861744543	2.9359019953540106
9	0.4184375699040751	2.165606059459582
>10	1.4040027895837994	11.272955651233003
>50	0.01710593839230496	0.6817648705706092
>100	0.014474255562719581	2.144419137843625
>500	0.0013158414147926892	0.3912013741232019
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	517	0.3912013741232019	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	499	0.37758121022722974	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	426	0.3223438788713424	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	374	0.2829967387274227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	352	0.26634987174345665	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	242	0.18311553682362644	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	240	0.18160218527962954	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	239	0.1808455095076311	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	141	0.10669128385178235	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.566757719984564E-4	0.0	0.0	0.29434687530739956	0.0
2	7.566757719984564E-4	0.0	0.0	1.5481586295088419	0.0
3	7.566757719984564E-4	0.0	0.0	2.01956763546388	0.0
4	7.566757719984564E-4	0.0	0.0	2.6634987174345666	0.0
5	7.566757719984564E-4	0.0	0.0	4.778407500170252	0.0
6	7.566757719984564E-4	0.0	0.0	5.677338317304418	0.0
7	7.566757719984564E-4	0.0	0.0	6.766194753210197	0.0
8	7.566757719984564E-4	0.0	0.0	8.837216341169972	0.0
9	7.566757719984564E-4	0.0	0.0	9.528817996776562	0.0
10	7.566757719984564E-4	0.0	0.0	11.800358664315928	0.0
11	7.566757719984564E-4	0.0	0.0	14.008338567007423	0.0
12	7.566757719984564E-4	0.0	0.0	16.16789122029102	0.0
13	7.566757719984564E-4	0.0	0.0	16.94651058967743	0.0
14	7.566757719984564E-4	0.0	0.0	17.218157191824876	0.0
15	7.566757719984564E-4	0.0	0.0	17.747830232223794	0.0
16	7.566757719984564E-4	0.0	0.0	18.738318817769773	0.0
17	7.566757719984564E-4	0.0	0.0	20.120008777438954	0.0
18	7.566757719984564E-4	0.0	0.0	21.67649084043978	0.0
19	7.566757719984564E-4	0.0	0.0	22.548181329782	0.0
20	7.566757719984564E-4	0.0	0.0	23.45619225618015	0.0
21	7.566757719984564E-4	0.0	0.0	24.408090377354206	0.0
22	7.566757719984564E-4	0.0	0.0	25.570344363143835	0.0
23	7.566757719984564E-4	0.0	0.0	26.707628048457515	0.0
24	7.566757719984564E-4	0.0	0.0	27.53921472188382	0.0
25	7.566757719984564E-4	0.0	0.0	28.224006295542424	0.0
26	7.566757719984564E-4	0.0	0.0	28.920148005781	0.0
27	7.566757719984564E-4	0.0	0.0	29.585266009367647	0.0
28	7.566757719984564E-4	0.0	0.0	30.251140688726288	0.0
29	7.566757719984564E-4	0.0	0.0	30.918528719628927	0.0
30	7.566757719984564E-4	0.0	0.0	31.73195517452727	0.0
31	7.566757719984564E-4	0.0	0.0	32.459120591417786	0.0
32	7.566757719984564E-4	0.0	0.0	33.1393721104444	0.0
33	7.566757719984564E-4	0.0	0.0	33.766656325431114	0.0
34	7.566757719984564E-4	0.0	0.0	34.433287680561754	0.0
35	7.566757719984564E-4	0.0	0.0	35.158183070136275	0.0
36	7.566757719984564E-4	0.0	0.0	35.773360472771024	0.0
37	7.566757719984564E-4	0.0	0.0	36.409724797021724	0.0
38	7.566757719984564E-4	0.0	0.0	37.0074986569005	0.0
39	7.566757719984564E-4	0.0	0.0	37.67942674243513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACTTA	45	3.8016879E-10	45.000004	5
TTAGGCG	20	7.0106203E-4	45.0	5
TTGGCCG	25	3.8720304E-5	45.0	36
GCGACGT	25	3.8720304E-5	45.0	17
CTCGTCC	25	3.8720304E-5	45.0	37
CGACGTC	25	3.8720304E-5	45.0	18
GGCCGCC	25	3.8720304E-5	45.0	38
GTCTCAC	20	7.0106203E-4	45.0	22
GTAGGGT	25	3.8720304E-5	45.0	4
AGGGTAC	25	3.8720304E-5	45.0	6
CATTCTA	25	3.8720304E-5	45.0	45
CAACAAG	20	7.0106203E-4	45.0	2
AAGCGAC	25	3.8720304E-5	45.0	15
CCTCGTC	25	3.8720304E-5	45.0	36
GCGTTAG	20	7.0106203E-4	45.0	1
TGGCCGC	25	3.8720304E-5	45.0	37
CGAGGGT	20	7.0106203E-4	45.0	4
TCGTCCC	25	3.8720304E-5	45.0	38
ACAGCGG	20	7.0106203E-4	45.0	2
AGCGACG	25	3.8720304E-5	45.0	16
>>END_MODULE