##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223941
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.999151562241842	33.0	31.0	34.0	30.0	34.0
2	32.138884795548826	33.0	31.0	34.0	30.0	34.0
3	31.82323915674218	33.0	31.0	34.0	30.0	34.0
4	35.649229038005544	37.0	35.0	37.0	33.0	37.0
5	35.817773431394876	37.0	35.0	37.0	35.0	37.0
6	35.83567100263016	37.0	35.0	37.0	35.0	37.0
7	26.87537342425014	36.0	10.0	37.0	0.0	37.0
8	31.253816853546248	37.0	19.0	37.0	17.0	37.0
9	36.088898415207574	39.0	32.0	39.0	32.0	39.0
10	36.76784063659625	38.0	35.0	39.0	33.0	39.0
11	37.257362430282974	39.0	37.0	39.0	34.0	39.0
12	37.345783041068856	39.0	37.0	39.0	34.0	39.0
13	37.34977962945597	39.0	37.0	39.0	34.0	39.0
14	38.587435976440226	40.0	38.0	41.0	35.0	41.0
15	38.65892802121987	40.0	38.0	41.0	35.0	41.0
16	38.56892663692669	40.0	38.0	41.0	34.0	41.0
17	38.47965758838266	40.0	38.0	41.0	34.0	41.0
18	38.35587051946718	40.0	38.0	41.0	34.0	41.0
19	38.22630960833434	40.0	37.0	41.0	34.0	41.0
20	38.25123581657669	40.0	37.0	41.0	34.0	41.0
21	38.19585962374018	40.0	37.0	41.0	34.0	41.0
22	38.15965365877619	40.0	37.0	41.0	34.0	41.0
23	38.11969224036688	40.0	37.0	41.0	34.0	41.0
24	38.07197431466324	40.0	37.0	41.0	34.0	41.0
25	37.96562487440888	40.0	37.0	41.0	33.0	41.0
26	37.88197784237813	40.0	36.0	41.0	33.0	41.0
27	37.78934183557276	40.0	36.0	41.0	33.0	41.0
28	37.73934205884586	40.0	36.0	41.0	33.0	41.0
29	37.72384690610473	40.0	36.0	41.0	33.0	41.0
30	37.626084549055335	40.0	36.0	41.0	33.0	41.0
31	37.49324152343698	40.0	36.0	41.0	33.0	41.0
32	37.33362805381775	40.0	36.0	41.0	32.0	41.0
33	37.180877999115836	40.0	36.0	41.0	31.0	41.0
34	36.96909900375545	40.0	36.0	41.0	31.0	41.0
35	36.80260872283325	40.0	35.0	41.0	30.0	41.0
36	36.61136638668221	39.0	35.0	41.0	30.0	41.0
37	36.54082101982218	39.0	35.0	41.0	30.0	41.0
38	36.42516555699939	39.0	35.0	41.0	30.0	41.0
39	36.40762522271491	39.0	35.0	41.0	30.0	41.0
40	36.27624686859485	39.0	35.0	41.0	29.0	41.0
41	36.1938814241251	39.0	35.0	41.0	29.0	41.0
42	36.14566783215222	39.0	35.0	41.0	28.0	41.0
43	36.09555641887819	39.0	35.0	41.0	28.0	41.0
44	35.922198257576774	39.0	35.0	41.0	28.0	41.0
45	35.84792869550462	39.0	35.0	40.0	27.0	41.0
46	35.73150070777571	39.0	35.0	40.0	27.0	41.0
47	35.625048561898	39.0	35.0	40.0	26.0	41.0
48	35.57332065142158	39.0	35.0	40.0	26.0	41.0
49	35.43786979606236	38.0	35.0	40.0	26.0	41.0
50	35.252727280846294	38.0	34.0	40.0	26.0	41.0
51	33.22212547054804	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	23.0
10	18.0
11	8.0
12	5.0
13	3.0
14	7.0
15	11.0
16	18.0
17	20.0
18	61.0
19	111.0
20	211.0
21	351.0
22	580.0
23	865.0
24	1423.0
25	1982.0
26	2728.0
27	3102.0
28	3182.0
29	3351.0
30	3943.0
31	4805.0
32	6292.0
33	8654.0
34	12651.0
35	16848.0
36	19824.0
37	33441.0
38	45713.0
39	53697.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.36984741516739	22.439839064753663	27.246462237821568	13.94385128225738
2	32.05040613375844	24.626129203674182	26.92360934353245	16.399855319034927
3	31.12114351547952	23.369548229221092	30.343706601292304	15.165601654007082
4	27.067843762419564	26.530202151459537	29.733277961605957	16.66867612451494
5	24.432328157863008	30.788020058854787	27.064271392911525	17.715380390370676
6	25.62014101928633	34.85069728187335	26.15421026073832	13.374951438102
7	61.87433297163092	27.44696147646032	7.318445483408577	3.360260068500185
8	85.28362381162896	3.952826860646331	8.155719586855465	2.607829740869247
9	77.65661491196342	8.742481278551047	9.844110725592902	3.7567930838926324
10	29.7730205723829	43.63247462501284	15.444246475634207	11.15025832697005
11	28.906720966683192	26.094819617667152	28.075251963686863	16.923207451962792
12	28.456155862481637	22.887278345635682	29.369342817974374	19.287222973908307
13	24.978454146404633	23.931749880548896	31.54000384029722	19.54979213274925
14	20.247743825382578	27.634064329443913	30.931361385364898	21.18683045980861
15	20.135214185879317	27.141523883522893	33.53472566434909	19.188536266248697
16	23.55575798982768	26.358281868885108	31.56233114972247	18.523628991564742
17	23.03240585689981	25.807690418458435	30.154817563554687	21.00508616108707
18	22.903800554610363	25.861275961079034	30.959047249052208	20.275876235258394
19	22.71580460924976	27.390696656708684	29.33495876145949	20.558539972582064
20	24.403302655610183	26.71953773538566	29.815442460290882	19.061717148713278
21	24.74982249789007	26.469471869822854	30.190987804823592	18.589717827463485
22	23.642834496586154	24.550662897816835	30.642446001402156	21.164056604194855
23	21.85575665018911	26.36453351552418	31.08050781232557	20.699202021961142
24	21.0073188920296	25.56789511523124	31.87134111216794	21.55344488057122
25	20.628647724177352	27.25717934634569	30.34504623985782	21.769126689619142
26	21.246221102879776	28.20742963548435	29.922166999343574	20.6241822622923
27	21.24488146431426	27.71533573575183	29.767662018120845	21.27212078181307
28	19.380551127305853	28.056943569958158	31.521695446568515	21.04080985616747
29	20.81932294666899	26.566372392728443	31.230100785474747	21.384203875127824
30	21.941493518382075	26.328809820443777	30.533488731406933	21.196207929767215
31	21.53736921778504	27.654605454115146	29.0862325344622	21.721792793637608
32	22.287566814473454	28.530282529773466	28.747750523575405	20.43440013217767
33	21.89549926096606	27.581371879200322	29.393009765965143	21.130119093868473
34	20.588905113400404	26.721323920139678	30.67950933504807	22.010261631411847
35	20.754127203147256	27.462144046869486	29.62432069161074	22.159408058372517
36	21.023394554815777	28.174385217534976	29.320669283427332	21.481550944221915
37	20.464318726807505	29.22689458384128	28.806694620458067	21.50209206889315
38	19.786461612656904	28.65352927780085	29.011659320981863	22.54834978856038
39	20.882285959248197	27.975672163650245	29.023716068071504	22.118325809030058
40	22.110734523825474	26.392665925399996	29.74086924681054	21.75573030396399
41	19.372066749724258	27.58539079489687	30.07220651868126	22.970335936697612
42	20.64472338696353	27.867161439843528	28.80535498189255	22.68276019130039
43	20.580867282007315	27.827418829066588	28.629862329810084	22.961851559116017
44	20.825128047119552	27.66576910882777	28.810713536154616	22.69838930789806
45	20.92828021666421	27.237084767862964	28.63477433788364	23.199860677589186
46	20.49736314475688	28.205196904541825	29.19652944302294	22.10091050767836
47	20.64293720220951	27.27950665577094	29.77436021094842	22.30319593107113
48	19.93248221629804	27.183945771430867	29.899839689918327	22.98373232235276
49	20.15664840292756	26.628442312930638	29.804278805578257	23.410630478563547
50	19.373406388289773	26.772230185629255	29.97307326483315	23.88129016124783
51	19.347060163167978	26.13992078270616	29.820354468364435	24.692664585761428
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	209.5
2	346.0
3	1966.5
4	3587.0
5	2475.5
6	1364.0
7	1146.5
8	929.0
9	878.0
10	827.0
11	808.5
12	790.0
13	773.0
14	756.0
15	746.0
16	736.0
17	708.0
18	680.0
19	724.5
20	769.0
21	889.0
22	1009.0
23	1125.5
24	1242.0
25	1568.0
26	2220.0
27	2546.0
28	3196.0
29	3846.0
30	4497.5
31	5149.0
32	5717.0
33	6285.0
34	7127.5
35	7970.0
36	8698.0
37	9426.0
38	10133.5
39	10841.0
40	11935.0
41	13029.0
42	13743.5
43	14458.0
44	15499.5
45	16541.0
46	17526.5
47	18512.0
48	18707.5
49	18903.0
50	17782.0
51	16661.0
52	15064.0
53	13467.0
54	12254.0
55	11041.0
56	10252.5
57	9464.0
58	9105.5
59	8747.0
60	8100.0
61	7453.0
62	6476.0
63	5499.0
64	4561.5
65	3624.0
66	2897.5
67	2171.0
68	1779.0
69	1387.0
70	1081.0
71	775.0
72	635.5
73	496.0
74	409.5
75	239.0
76	155.0
77	107.0
78	59.0
79	59.0
80	59.0
81	40.5
82	22.0
83	19.5
84	17.0
85	11.5
86	6.0
87	4.0
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	223941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.0171090421427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.6526363982366	30.867971644400367
2	12.690971028733683	11.933855374172909
3	6.881952431226561	9.707085236454548
4	4.254585970297827	8.001533299786535
5	2.7811536333665674	6.538090182147362
6	2.109933482254247	5.952178356408947
7	1.5265987997764443	5.024338356288528
8	1.1636438928316661	4.376893743639189
9	0.8216301733288051	3.4767607906543527
>10	2.098945958497045	12.441046207421627
>50	0.012962611603187566	0.4420961887650671
>100	0.003988495877903867	0.3797432798512241
>500	0.0	0.0
>1k	9.971239694759667E-4	0.8584073400093719
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1831	0.8176260711526696	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.14602060364113761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04554771122751082	0.0
2	0.0	0.0	0.0	0.2505124117513095	0.0
3	0.0	0.0	0.0	0.38760209162234693	0.0
4	0.0	0.0	0.0	0.5322830566979696	0.0
5	0.0	0.0	0.0	0.9618604900397872	0.0
6	0.0	0.0	0.0	1.3128457942047236	0.0
7	0.0	0.0	0.0	1.6437365198869345	0.0
8	0.0	0.0	0.0	2.340348573954747	0.0
9	0.0	0.0	0.0	2.6422137973841324	0.0
10	0.0	0.0	0.0	3.250856252316458	0.0
11	0.0	0.0	0.0	3.91531698081191	0.0
12	0.0	0.0	0.0	4.511009596277591	0.0
13	0.0	0.0	0.0	4.752144538070295	0.0
14	0.0	0.0	0.0	4.8615483542540225	0.0
15	0.0	0.0	0.0	5.049990845803135	0.0
16	0.0	0.0	0.0	5.421070728450798	0.0
17	0.0	0.0	0.0	5.908699166298266	0.0
18	0.0	0.0	0.0	6.39677415033424	0.0
19	0.0	0.0	0.0	6.688815357616515	0.0
20	0.0	0.0	0.0	7.017919898544706	0.0
21	0.0	0.0	0.0	7.410880544429113	0.0
22	0.0	0.0	0.0	7.856980186745616	0.0
23	0.0	0.0	0.0	8.264676856850688	0.0
24	0.0	0.0	0.0	8.61253633769609	0.0
25	0.0	0.0	0.0	8.929584131534645	0.0
26	0.0	0.0	0.0	9.20554967603074	0.0
27	0.0	0.0	0.0	9.479729035772815	0.0
28	0.0	0.0	0.0	9.780254620636686	0.0
29	0.0	0.0	0.0	10.089711129270656	0.0
30	0.0	0.0	0.0	10.471954666630943	0.0
31	0.0	0.0	0.0	10.80775740038671	0.0
32	0.0	0.0	0.0	11.139094672257425	0.0
33	0.0	0.0	0.0	11.4489977270799	0.0
34	0.0	0.0	0.0	11.801769215998856	0.0
35	0.0	0.0	0.0	12.176421468154558	0.0
36	0.0	0.0	0.0	12.54928753555624	0.0
37	0.0	0.0	0.0	12.89000227738556	0.0
38	0.0	0.0	0.0	13.228930834460863	0.0
39	0.0	0.0	0.0	13.605815817559089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	20	7.021109E-4	45.000004	4
CGACAAT	20	7.021109E-4	45.000004	20
AAACGCG	20	7.021109E-4	45.000004	1
GCGATCA	20	7.021109E-4	45.000004	9
GCGATAT	20	7.021109E-4	45.000004	9
GCGATAG	40	6.7720975E-9	45.000004	9
TTACGAG	20	7.021109E-4	45.000004	1
GGCGCTA	20	7.021109E-4	45.000004	1
TGCAACG	20	7.021109E-4	45.000004	2
CTACGAA	25	3.8807164E-5	45.0	11
AAGGGCG	70	0.0	45.0	5
GCTACGA	25	3.8807164E-5	45.0	10
CGTTTTA	105	0.0	42.857143	1
CGTTTTT	805	0.0	42.20497	1
GGCGATA	80	0.0	42.187504	8
CGGTTTT	100	0.0	40.5	1
GTAAGGA	90	0.0	40.0	4
CGCATGG	40	3.441619E-7	39.375004	2
TAACGGG	75	0.0	39.0	3
CGGGAAT	75	0.0	39.0	6
>>END_MODULE