##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258620
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06270203387209	33.0	31.0	34.0	30.0	34.0
2	32.19674039130771	33.0	31.0	34.0	30.0	34.0
3	31.780856082282885	33.0	31.0	34.0	30.0	34.0
4	35.654431211816565	37.0	35.0	37.0	33.0	37.0
5	35.845503054674815	37.0	35.0	37.0	35.0	37.0
6	35.871823524862734	37.0	35.0	37.0	35.0	37.0
7	27.01661124429665	36.0	11.0	37.0	0.0	37.0
8	31.32729100610935	37.0	23.0	37.0	17.0	37.0
9	36.1332572886861	39.0	32.0	39.0	32.0	39.0
10	36.8776660737762	38.0	37.0	39.0	34.0	39.0
11	37.30286520764055	39.0	37.0	39.0	34.0	39.0
12	37.3486234629959	39.0	37.0	39.0	35.0	39.0
13	37.32700487201299	39.0	37.0	39.0	34.0	39.0
14	38.472635527028075	40.0	38.0	41.0	34.0	41.0
15	38.561074162864436	40.0	38.0	41.0	34.0	41.0
16	38.53332302219473	40.0	38.0	41.0	34.0	41.0
17	38.46033176088469	40.0	38.0	41.0	34.0	41.0
18	38.29894826386204	40.0	38.0	41.0	34.0	41.0
19	38.12639007037352	40.0	37.0	41.0	34.0	41.0
20	38.10687108498956	40.0	37.0	41.0	34.0	41.0
21	38.03659423091795	40.0	36.0	41.0	34.0	41.0
22	38.05586574897533	40.0	36.0	41.0	34.0	41.0
23	37.995553321475526	40.0	36.0	41.0	34.0	41.0
24	37.94853452942541	40.0	36.0	41.0	34.0	41.0
25	37.84057304152811	40.0	36.0	41.0	33.0	41.0
26	37.72834660892429	40.0	36.0	41.0	33.0	41.0
27	37.63065501508004	40.0	36.0	41.0	33.0	41.0
28	37.5981710617895	40.0	35.0	41.0	33.0	41.0
29	37.53744876653004	40.0	35.0	41.0	33.0	41.0
30	37.43377542340113	40.0	35.0	41.0	33.0	41.0
31	37.27484726625938	40.0	35.0	41.0	32.0	41.0
32	37.051995205320544	39.0	35.0	41.0	32.0	41.0
33	36.86427963807903	39.0	35.0	41.0	31.0	41.0
34	36.59092104245612	39.0	35.0	41.0	30.0	41.0
35	36.3675431134483	39.0	35.0	41.0	30.0	41.0
36	36.17680380481015	39.0	35.0	41.0	29.0	41.0
37	36.07610780295414	39.0	35.0	41.0	29.0	41.0
38	35.86146856391617	39.0	35.0	41.0	27.0	41.0
39	35.76290310107493	39.0	35.0	41.0	26.0	41.0
40	35.59036810764829	39.0	35.0	41.0	25.0	41.0
41	35.48276622070992	39.0	35.0	41.0	25.0	41.0
42	35.40435387827701	39.0	35.0	41.0	24.0	41.0
43	35.36707524553399	39.0	35.0	40.0	24.0	41.0
44	35.145359987626634	39.0	35.0	40.0	23.0	41.0
45	35.02161085762896	38.0	34.0	40.0	23.0	41.0
46	34.91574124197665	38.0	34.0	40.0	23.0	41.0
47	34.76700177867141	38.0	34.0	40.0	23.0	41.0
48	34.68902250406001	38.0	34.0	40.0	23.0	41.0
49	34.581787951434535	38.0	34.0	40.0	22.0	41.0
50	34.38724383265022	38.0	34.0	40.0	21.0	41.0
51	32.473903797076794	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	14.0
10	19.0
11	18.0
12	9.0
13	6.0
14	11.0
15	7.0
16	24.0
17	44.0
18	110.0
19	160.0
20	290.0
21	519.0
22	793.0
23	1298.0
24	2037.0
25	2997.0
26	4037.0
27	4491.0
28	4498.0
29	4620.0
30	5061.0
31	6013.0
32	7570.0
33	10318.0
34	14991.0
35	19735.0
36	22427.0
37	37309.0
38	49980.0
39	59193.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.47420926455804	20.70257520686722	26.030469414585106	14.792746113989638
2	35.10749361998299	23.172995127987008	25.428814476838607	16.290696775191403
3	30.899389065037507	22.869460985229296	30.559894826386202	15.671255123346995
4	26.73845796922125	25.0556801484804	30.593534916093105	17.612326966205245
5	23.970690588508237	29.264171371123656	28.98190395174387	17.783234088624237
6	25.868068981517283	33.48271595390921	27.260846028922742	13.388369035650763
7	60.95236253963344	27.143299048797466	8.789343438249169	3.114994973319929
8	83.16526177403139	4.26649137731034	10.059546825458202	2.508700023200062
9	76.2330832882221	8.063568169515118	11.762431366483645	3.940917175779136
10	33.35936895831722	38.16216843244915	17.401593070914856	11.07686953831877
11	29.56847884927693	25.121800324800862	28.574742865980973	16.734977959941226
12	28.305622148325725	22.642100378934344	30.750522001392007	18.301755471347924
13	25.844482251952673	23.31451550537468	32.14677905807749	18.69422318459516
14	21.065656175083134	25.389374371664992	33.10648828396875	20.43848116928312
15	20.136493697316528	25.415281107416288	34.96442657180419	19.483798623462995
16	25.167813780836752	25.844095584254887	31.253963343902253	17.73412729100611
17	24.747119325651536	25.01624004330678	30.80929549145464	19.42734513958704
18	25.317454179877814	23.693836516897377	31.573737529966746	19.41497177325806
19	23.951743871316992	26.46779058077488	29.992266646044392	19.588198901863738
20	25.39594772252726	26.304230144613722	30.266414043770784	18.033408089088237
21	24.54295878122342	25.884309024824066	31.676591137576366	17.89614105637615
22	24.506225349934265	23.456422550460136	32.140205707215216	19.89714639239038
23	21.61356430283814	25.29657412419766	32.62818034181424	20.46168123114995
24	21.307710153893744	25.100533601422935	32.64867372979661	20.943082514886708
25	22.244219317918184	26.748897997061327	30.540174773799393	20.466707911221096
26	21.73691129843013	26.757018018714717	30.038666769778054	21.467403913077103
27	20.912149099064266	26.54512412033099	31.545897455726546	20.9968293248782
28	19.710385894362386	26.858324955533213	31.845178253808676	21.586110896295725
29	20.987935967829248	26.264016704044547	31.734977959941226	21.01306936818498
30	22.77859407625087	25.367334312891497	31.559430825148866	20.29464078570876
31	23.04268811383497	26.307710153893744	30.57072152192406	20.07888021034723
32	23.365555641481713	26.282963421235788	30.52741473977264	19.82406619750986
33	23.41659577758874	26.178949810532824	30.04137344366252	20.363080968215915
34	21.498723996597324	26.657644420385125	31.873018328048875	19.97061325496868
35	21.366483643956382	27.73567396179723	30.35882762354033	20.539014770706054
36	22.466166576444206	28.560049493465318	28.538009434691823	20.435774495398654
37	22.20787255432681	28.251102002938676	29.21699791199443	20.324027530740082
38	22.01724537932101	28.46376923671797	29.240971309256825	20.2780140747042
39	21.87688500502668	26.81385817028845	29.09558425489135	22.21367256979352
40	23.742943314515504	26.383497022658727	29.389065037506768	20.484494625318998
41	21.386977031938752	26.594230917949112	29.175237800634136	22.843554249478
42	21.39625705668548	26.929858479622617	29.368184981826616	22.305699481865286
43	21.54164411105096	26.873404995746657	29.938519836052897	21.646431057149485
44	21.89196504524012	26.36261696697858	29.770319387518366	21.975098600262935
45	21.521537390766376	26.391230376614338	29.351944938519836	22.73528729409945
46	21.14414971773258	27.52880674348465	29.118397649060395	22.208645889722373
47	20.44466785244761	27.386899698399198	30.462841234243292	21.705591214909905
48	20.800788802103472	27.429046477457277	30.616348310262158	21.153816410177093
49	21.940298507462686	26.28412342432913	29.718505916015776	22.057072152192404
50	20.1906271750058	27.437939834506224	30.114840306240815	22.25659268424716
51	20.55564148171062	26.57721753924677	29.453638543036114	23.413502436006496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	233.0
2	375.0
3	3183.5
4	5992.0
5	4028.5
6	2065.0
7	1734.0
8	1403.0
9	1355.0
10	1307.0
11	1298.0
12	1289.0
13	1196.5
14	1104.0
15	1071.0
16	1038.0
17	974.0
18	910.0
19	922.0
20	934.0
21	1035.0
22	1136.0
23	1308.0
24	1480.0
25	1781.0
26	2517.0
27	2952.0
28	3326.0
29	3700.0
30	4395.0
31	5090.0
32	6063.0
33	7036.0
34	8074.0
35	9112.0
36	9601.5
37	10091.0
38	10950.5
39	11810.0
40	12586.5
41	13363.0
42	14024.0
43	14685.0
44	15560.0
45	16435.0
46	17567.5
47	18700.0
48	19239.5
49	19779.0
50	19651.5
51	19524.0
52	18299.5
53	17075.0
54	15838.5
55	14602.0
56	13704.5
57	12807.0
58	11720.0
59	10633.0
60	9908.0
61	9183.0
62	8083.0
63	6983.0
64	5845.0
65	4707.0
66	4033.0
67	3359.0
68	2791.0
69	2223.0
70	1831.0
71	1439.0
72	1139.5
73	840.0
74	690.5
75	448.5
76	356.0
77	284.5
78	213.0
79	146.0
80	79.0
81	64.5
82	50.0
83	35.5
84	21.0
85	18.0
86	15.0
87	9.0
88	3.0
89	4.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.73323250932877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.15598687654042	35.08506761619615
2	11.45817595655875	11.626206098737914
3	6.36774961507089	9.69169565347741
4	4.042341083873658	8.203241203606975
5	2.7348510217946074	6.937391638354061
6	1.9257761429388172	5.862050929238004
7	1.3303129952336967	4.7243754948160905
8	0.9242078423627608	3.751044108282802
9	0.6432385358429124	2.937021317809082
>10	1.403658345527502	8.988012120954442
>50	0.009134389503981372	0.3197578035100189
>100	0.0030447965013271237	0.3919736359452527
>500	7.611991253317809E-4	0.21819222099415544
>1k	7.611991253317809E-4	1.2639701580776475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3273	1.2655633748356663	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.21846724924599797	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	422	0.16317376846338255	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.12218699249864666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05374680999149331	0.0
2	0.0	0.0	0.0	0.1917871780991416	0.0
3	0.0	0.0	0.0	0.2872940994509319	0.0
4	0.0	0.0	0.0	0.3858943623849664	0.0
5	0.0	0.0	0.0	0.6685484494625319	0.0
6	0.0	0.0	0.0	0.9318691516510711	0.0
7	0.0	0.0	0.0	1.178176475137267	0.0
8	0.0	0.0	0.0	1.617430979815946	0.0
9	0.0	0.0	0.0	1.8946717191245843	0.0
10	0.0	0.0	0.0	2.3938597169592453	0.0
11	0.0	0.0	0.0	2.926687804500812	0.0
12	0.0	0.0	0.0	3.3558889490371975	0.0
13	0.0	0.0	0.0	3.551542804114144	0.0
14	0.0	0.0	0.0	3.6578764210037895	0.0
15	0.0	0.0	0.0	3.803263475369268	0.0
16	0.0	0.0	0.0	4.051117469646586	0.0
17	0.0	0.0	0.0	4.357358286288764	0.0
18	0.0	0.0	0.0	4.716185909829093	0.0
19	0.0	0.0	0.0	4.939293171448457	0.0
20	0.0	0.0	0.0	5.184440491841311	0.0
21	0.0	0.0	0.0	5.455881215683242	0.0
22	0.0	0.0	0.0	5.768695383187689	0.0
23	0.0	0.0	0.0	6.074162864434305	0.0
24	0.0	0.0	0.0	6.3243368648983065	0.0
25	0.0	0.0	0.0	6.536230763282036	0.0
26	0.0	0.0	0.0	6.744644652385739	0.0
27	0.0	0.0	0.0	7.005258680689815	0.0
28	0.0	0.0	0.0	7.24344598252262	0.0
29	0.0	0.0	0.0	7.4974866599644265	0.0
30	0.0	0.0	0.0	7.8466475910602425	0.0
31	0.0	0.0	0.0	8.129301678137809	0.0
32	0.0	0.0	0.0	8.407702420539788	0.0
33	0.0	0.0	0.0	8.667929781146084	0.0
34	0.0	0.0	0.0	8.95986389297038	0.0
35	0.0	0.0	0.0	9.26765138040368	0.0
36	0.0	0.0	0.0	9.562678833810223	0.0
37	0.0	0.0	0.0	9.8669863119635	0.0
38	0.0	0.0	0.0	10.224653932410487	0.0
39	0.0	0.0	0.0	10.76212203232542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.023136E-4	45.000004	16
CCGCGGT	20	7.023136E-4	45.000004	11
CGGTCAT	20	7.023136E-4	45.000004	14
TAGGACA	20	7.023136E-4	45.000004	6
AATACGG	20	7.023136E-4	45.000004	2
CGGTTTA	25	3.882395E-5	45.0	1
CGGTCTA	50	2.1827873E-11	45.0	31
CGTTTTT	1205	0.0	42.94606	1
GCTACGA	70	0.0	41.785713	10
CGACGGT	55	6.002665E-11	40.909092	28
GGACCGA	145	0.0	40.344826	8
AGGCGAT	95	0.0	40.26316	7
ATCTACC	45	1.9183062E-8	40.0	15
GACCGAT	135	0.0	40.0	9
CGTTTTA	205	0.0	39.512196	1
TATTGCG	40	3.4443838E-7	39.375004	1
GTTACGG	40	3.4443838E-7	39.375004	2
GGCGATA	40	3.4443838E-7	39.375004	8
TACGAAT	75	0.0	39.0	12
CATGCGG	35	6.2295276E-6	38.571426	2
>>END_MODULE