FastQCFastQC Report
Sat 14 Jan 2017
SRR2936384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268846
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.26334779018471544No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC3860.14357662007245783No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG3290.12237489120165447No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC3150.11716744902286068No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC2780.10340492326461989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG351.2072815E-745.0000041
CCTCGTC207.023635E-445.036
TAGGCGG207.023635E-445.02
CGGTCTA650.041.5384631
CCCGCAC556.002665E-1140.90909233
ATACGGG501.0750227E-940.5000043
TCTCACG800.039.37523
TGCGTAA403.4450568E-739.3751
CTCACGA750.039.024
ATCAACG750.039.015
CGGGCTT356.2304443E-638.571436
GACGGTC700.038.5714329
AGCCCGG301.1375966E-437.49999630
ACCCGCA601.546141E-1037.49999632
TACGGGA1050.036.428574
CGTTTTT3350.036.2686581
GTGCAAG250.002104243736.0000042
CTCGTCC250.002104243736.00000437
CGCATGT250.002104243736.00000437
CTAACGG250.002104243736.0000042
Produced by FastQC (version 0.11.5)