##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936384.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268846
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03717369795348	33.0	31.0	34.0	30.0	34.0
2	32.170186649606094	33.0	31.0	34.0	30.0	34.0
3	32.05071304761834	33.0	31.0	34.0	30.0	34.0
4	35.77817412198805	37.0	35.0	37.0	35.0	37.0
5	35.86216272512888	37.0	35.0	37.0	35.0	37.0
6	35.85326543820626	37.0	35.0	37.0	35.0	37.0
7	26.929227141188637	36.0	11.0	37.0	0.0	37.0
8	31.25301845666292	37.0	19.0	37.0	17.0	37.0
9	36.05319030225482	39.0	32.0	39.0	32.0	39.0
10	36.6831866570453	38.0	35.0	39.0	33.0	39.0
11	37.16802556110189	39.0	37.0	39.0	34.0	39.0
12	37.24334749261659	39.0	37.0	39.0	34.0	39.0
13	37.19173802102319	39.0	37.0	39.0	34.0	39.0
14	38.33668345446836	40.0	38.0	41.0	34.0	41.0
15	38.42955446612559	40.0	38.0	41.0	34.0	41.0
16	38.3613444127865	40.0	38.0	41.0	34.0	41.0
17	38.270459668360324	40.0	38.0	41.0	34.0	41.0
18	38.18884789061396	40.0	37.0	41.0	33.0	41.0
19	38.09418775060816	40.0	37.0	41.0	34.0	41.0
20	38.133927973635465	40.0	37.0	41.0	34.0	41.0
21	38.07557858402208	40.0	37.0	41.0	33.0	41.0
22	38.0938604256712	40.0	37.0	41.0	34.0	41.0
23	38.02894221970943	40.0	37.0	41.0	34.0	41.0
24	37.99252732047343	40.0	37.0	41.0	33.0	41.0
25	37.90574901616539	40.0	37.0	41.0	33.0	41.0
26	37.79294465976805	40.0	37.0	41.0	33.0	41.0
27	37.653132276470544	40.0	36.0	41.0	33.0	41.0
28	37.615828392462596	40.0	36.0	41.0	33.0	41.0
29	37.57823809913482	40.0	36.0	41.0	33.0	41.0
30	37.46172901958742	40.0	36.0	41.0	32.0	41.0
31	37.36440936446888	40.0	36.0	41.0	32.0	41.0
32	37.232155211533744	40.0	36.0	41.0	31.0	41.0
33	37.144595790898876	39.0	36.0	41.0	31.0	41.0
34	37.03664179493093	39.0	36.0	41.0	31.0	41.0
35	36.957496113016376	39.0	35.0	41.0	31.0	41.0
36	36.79794752386125	39.0	35.0	41.0	30.0	41.0
37	36.715536031780275	39.0	35.0	41.0	30.0	41.0
38	36.586324512918175	39.0	35.0	41.0	30.0	41.0
39	36.53533621478467	39.0	35.0	41.0	30.0	41.0
40	36.396881486055214	39.0	35.0	41.0	30.0	41.0
41	36.31399016537348	39.0	35.0	41.0	30.0	41.0
42	36.244251355794766	39.0	35.0	40.0	29.0	41.0
43	36.2051546238367	39.0	35.0	40.0	29.0	41.0
44	36.03855367013085	39.0	35.0	40.0	29.0	41.0
45	35.94933530720189	39.0	35.0	40.0	28.0	41.0
46	35.856921806536086	38.0	35.0	40.0	28.0	41.0
47	35.71278352662863	38.0	35.0	40.0	28.0	41.0
48	35.59627444708123	38.0	35.0	40.0	27.0	41.0
49	35.44752386124399	38.0	34.0	40.0	27.0	41.0
50	35.23600499914449	38.0	34.0	40.0	26.0	41.0
51	33.05095854132105	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	19.0
10	17.0
11	12.0
12	10.0
13	6.0
14	5.0
15	15.0
16	16.0
17	42.0
18	91.0
19	158.0
20	311.0
21	456.0
22	684.0
23	1039.0
24	1436.0
25	1925.0
26	2481.0
27	3032.0
28	3544.0
29	3993.0
30	5128.0
31	6528.0
32	8397.0
33	11134.0
34	16490.0
35	20842.0
36	25467.0
37	41087.0
38	53922.0
39	60534.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.585487602568016	21.741071096464147	27.532862679749748	11.140578621218095
2	30.375382189059906	25.071602329958413	27.600187467918435	16.95282801306324
3	31.698072502473533	24.76845480312149	27.339815358978747	16.193657335426227
4	28.075552546811185	25.862761580979445	27.883249146351442	18.178436725857928
5	25.496380827685737	30.916584215498837	24.942160195799826	18.6448747610156
6	26.173720271084562	35.765456804267124	23.654806097170873	14.40601682747744
7	63.51219657350305	27.41048778854809	5.491619737693698	3.585695900255165
8	87.1283188144886	4.229558929647456	5.7475283247658515	2.894593931098101
9	79.76239185258474	8.205812993312156	7.422464905559316	4.609330248543777
10	33.43810211050192	40.844572729369226	13.305758687129435	12.411566472999413
11	30.567685589519648	25.80808343810211	25.115493628322533	18.508737344055703
12	29.61397975048913	21.74218697693103	28.000788555529933	20.64304471704991
13	26.197525721044762	23.668196662773482	29.564881009946216	20.56939660623554
14	21.37245858223667	26.776295723202132	29.47226293119481	22.378982763366388
15	20.739754357513224	26.168884789061398	32.360533539647236	20.730827313778146
16	24.781845368724102	25.750429613979755	29.291118335403908	20.17660668189224
17	24.808998460084954	24.625994063515915	28.003392276619326	22.5616151997798
18	24.562760837059134	24.81941334444254	29.265453084665573	21.352372733832752
19	24.445593388036276	26.30985768804446	26.97231872521815	22.272230198701116
20	25.45658109103353	26.71231857643409	27.529143078193464	20.301957254338916
21	25.844535533353667	25.77646682487372	28.48656851878027	19.892429122992343
22	24.986051494163945	23.009455227156067	29.045996592844975	22.958496685835016
23	22.161386072323932	25.34350520372258	29.70325018783988	22.79185853611361
24	22.924276351517225	25.162732568087304	29.36513840637391	22.54785267402156
25	22.419526420329856	27.087626373462875	27.339815358978747	23.153031847228526
26	21.5989823170142	27.35878532691578	27.51649643290211	23.525735923167908
27	21.73846737537475	26.974550486151923	28.598900485779964	22.688081652693366
28	20.67689309121207	26.97306264552941	29.129687627861305	23.220356635397216
29	21.503016596862143	25.600157711105986	28.98797080856699	23.908854883464883
30	22.44891127262448	26.55386355013651	28.71011657231277	22.28710860492624
31	23.54173020985992	25.97695334875728	27.562619492199996	22.918696949182802
32	24.169226992404575	26.377182476213147	27.31526598870729	22.13832454267499
33	23.387366745274246	26.467940754186415	27.61171823274291	22.532974267796433
34	21.739211295686005	25.686452467211712	28.94779911175915	23.626537125343134
35	21.841128378328115	26.3422182215841	28.27566711053912	23.540986289548663
36	23.41042827492319	26.24922818267707	28.136554012334198	22.20378953006554
37	21.93560625785766	27.591632384338986	28.261532624625254	22.2112287331781
38	22.644934274640498	27.31377814808478	27.59200434449462	22.449283232780104
39	21.95755190703972	25.799156394367035	28.422591372012228	23.82070032658102
40	22.885964455487528	25.68087306487729	28.91581053837513	22.517351941260053
41	21.123245277965825	25.78836954985382	28.33778445652902	24.750600715651338
42	21.934862337546402	25.103218943186807	29.038185429576785	23.92373328969001
43	22.201929729287396	25.875408226270803	28.465738750065096	23.456923294376704
44	21.79016983700706	25.651488212582667	28.878614522812317	23.679727427597953
45	21.760041064401182	25.272460813997604	27.612834113209793	25.35466400839142
46	22.322072859555284	26.32510805442521	27.7861675457325	23.566651540287005
47	20.785133496499856	26.11829820789597	29.69283530348229	23.403732992121885
48	21.629855009931337	25.883219389538993	29.06831420218266	23.418611398347007
49	21.85823854548701	24.610371736979538	30.159645298795596	23.371744418737865
50	20.895233702565783	25.560729934609405	29.39266345789039	24.151372904934423
51	20.663130565453827	24.416952456052908	28.81984481822307	26.100072160270194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	239.5
2	392.0
3	938.0
4	1484.0
5	1023.0
6	562.0
7	499.0
8	436.0
9	428.5
10	421.0
11	438.5
12	456.0
13	438.0
14	420.0
15	454.0
16	488.0
17	462.5
18	437.0
19	467.5
20	498.0
21	585.5
22	673.0
23	856.5
24	1040.0
25	1388.5
26	2098.5
27	2460.0
28	3038.0
29	3616.0
30	4361.0
31	5106.0
32	5889.5
33	6673.0
34	7678.0
35	8683.0
36	9485.0
37	10287.0
38	11071.5
39	11856.0
40	12548.0
41	13240.0
42	14653.5
43	16067.0
44	17370.0
45	18673.0
46	21097.5
47	23522.0
48	23689.5
49	23857.0
50	22652.5
51	21448.0
52	19755.5
53	18063.0
54	16626.5
55	15190.0
56	14571.5
57	13953.0
58	12969.0
59	11985.0
60	11192.5
61	10400.0
62	9357.0
63	8314.0
64	6995.5
65	5677.0
66	4882.0
67	4087.0
68	3426.0
69	2765.0
70	2212.5
71	1660.0
72	1278.0
73	896.0
74	729.0
75	427.5
76	293.0
77	221.0
78	149.0
79	128.5
80	108.0
81	85.0
82	62.0
83	47.0
84	32.0
85	25.5
86	19.0
87	13.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.69106186832551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.95073910532486	32.19383225433494
2	10.396075513250736	9.708076099539479
3	5.669422301243545	7.941340422750802
4	3.8150348496289066	7.125121127753648
5	2.734520315551817	6.383882861681143
6	2.0477626667567512	5.736732801911201
7	1.5284916202566283	4.995682776463354
8	1.1972557718136208	4.472091465116765
9	0.9479308071660438	3.9833906367853347
>10	2.6945072972923643	16.170248997834037
>50	0.010320729230065615	0.33546665795503255
>100	0.007145120236199272	0.6916914737503767
>500	7.939022484665857E-4	0.2624424241239371
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.26334779018471544	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC	386	0.14357662007245783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG	329	0.12237489120165447	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC	315	0.11716744902286068	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	278	0.10340492326461989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06918458894683202	0.0
2	0.0	0.0	0.0	0.38386288060822926	0.0
3	0.0	0.0	0.0	0.50698169212114	0.0
4	0.0	0.0	0.0	0.6966813714914858	0.0
5	0.0	0.0	0.0	1.3052081860991052	0.0
6	0.0	0.0	0.0	1.6343929238299992	0.0
7	0.0	0.0	0.0	1.9602300201602405	0.0
8	0.0	0.0	0.0	2.6342218221584104	0.0
9	0.0	0.0	0.0	2.889386488919307	0.0
10	0.0	0.0	0.0	3.664551453248328	0.0
11	0.0	0.0	0.0	4.40772784419333	0.0
12	0.0	0.0	0.0	5.204466497548783	0.0
13	0.0	0.0	0.0	5.510217745475105	0.0
14	0.0	0.0	0.0	5.644867321812487	0.0
15	0.0	0.0	0.0	5.869903215967506	0.0
16	0.0	0.0	0.0	6.283894869181613	0.0
17	0.0	0.0	0.0	6.796084003481547	0.0
18	0.0	0.0	0.0	7.3696465634601225	0.0
19	0.0	0.0	0.0	7.718173229283679	0.0
20	0.0	0.0	0.0	8.125841559852109	0.0
21	0.0	0.0	0.0	8.578145109095914	0.0
22	0.0	0.0	0.0	9.076943677793235	0.0
23	0.0	0.0	0.0	9.572394605089903	0.0
24	0.0	0.0	0.0	9.959605127098785	0.0
25	0.0	0.0	0.0	10.318174717124302	0.0
26	0.0	0.0	0.0	10.650335136100221	0.0
27	0.0	0.0	0.0	11.00258140348006	0.0
28	0.0	0.0	0.0	11.381980762220751	0.0
29	0.0	0.0	0.0	11.754684838160136	0.0
30	0.0	0.0	0.0	12.232653638142283	0.0
31	0.0	0.0	0.0	12.640321968710712	0.0
32	0.0	0.0	0.0	13.016745646206378	0.0
33	0.0	0.0	0.0	13.384614240122598	0.0
34	0.0	0.0	0.0	13.772568682442737	0.0
35	0.0	0.0	0.0	14.211481666083928	0.0
36	0.0	0.0	0.0	14.606131391205373	0.0
37	3.719601556281291E-4	0.0	0.0	15.01045208037315	0.0
38	3.719601556281291E-4	0.0	0.0	15.456432306971278	0.0
39	3.719601556281291E-4	0.0	0.0	15.985359648274477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAG	35	1.2072815E-7	45.000004	1
CCTCGTC	20	7.023635E-4	45.0	36
TAGGCGG	20	7.023635E-4	45.0	2
CGGTCTA	65	0.0	41.53846	31
CCCGCAC	55	6.002665E-11	40.909092	33
ATACGGG	50	1.0750227E-9	40.500004	3
TCTCACG	80	0.0	39.375	23
TGCGTAA	40	3.4450568E-7	39.375	1
CTCACGA	75	0.0	39.0	24
ATCAACG	75	0.0	39.0	15
CGGGCTT	35	6.2304443E-6	38.57143	6
GACGGTC	70	0.0	38.57143	29
AGCCCGG	30	1.1375966E-4	37.499996	30
ACCCGCA	60	1.546141E-10	37.499996	32
TACGGGA	105	0.0	36.42857	4
CGTTTTT	335	0.0	36.268658	1
GTGCAAG	25	0.0021042437	36.000004	2
CTCGTCC	25	0.0021042437	36.000004	37
CGCATGT	25	0.0021042437	36.000004	37
CTAACGG	25	0.0021042437	36.000004	2
>>END_MODULE