##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108848
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85980449801558	31.0	31.0	34.0	30.0	34.0
2	31.99124467146847	33.0	31.0	34.0	30.0	34.0
3	31.826106129648686	33.0	31.0	34.0	30.0	34.0
4	35.63121968249302	37.0	35.0	37.0	33.0	37.0
5	35.73137770101426	37.0	35.0	37.0	35.0	37.0
6	35.727316992503305	37.0	35.0	37.0	35.0	37.0
7	26.983582610612967	35.0	16.0	37.0	0.0	37.0
8	31.248667867117447	35.0	23.0	37.0	17.0	37.0
9	35.981607379097454	39.0	32.0	39.0	32.0	39.0
10	36.59957004262825	37.0	35.0	39.0	32.0	39.0
11	37.000440981919745	39.0	37.0	39.0	34.0	39.0
12	37.09154968396296	39.0	37.0	39.0	34.0	39.0
13	37.037501837424664	39.0	37.0	39.0	33.0	39.0
14	38.18877701014259	40.0	37.0	41.0	33.0	41.0
15	38.24959576657357	40.0	37.0	41.0	33.0	41.0
16	38.19438115537263	40.0	37.0	41.0	34.0	41.0
17	38.09015324121711	40.0	37.0	41.0	33.0	41.0
18	38.00895744524475	40.0	37.0	41.0	33.0	41.0
19	37.87782963398501	40.0	37.0	41.0	33.0	41.0
20	37.98479531089225	40.0	37.0	41.0	34.0	41.0
21	37.95742687049831	40.0	37.0	41.0	33.0	41.0
22	37.978263266206085	40.0	37.0	41.0	33.0	41.0
23	37.94303983536675	40.0	37.0	41.0	33.0	41.0
24	37.91248346317801	40.0	36.0	41.0	33.0	41.0
25	37.847401881522856	40.0	36.0	41.0	33.0	41.0
26	37.722218139056295	39.0	36.0	41.0	33.0	41.0
27	37.645340291048065	39.0	36.0	41.0	33.0	41.0
28	37.656668014111425	40.0	36.0	41.0	33.0	41.0
29	37.634380052917834	40.0	36.0	41.0	33.0	41.0
30	37.600415257974426	40.0	36.0	41.0	33.0	41.0
31	37.46819417903866	39.0	36.0	41.0	33.0	41.0
32	37.35126966044392	39.0	36.0	41.0	33.0	41.0
33	37.260794869910335	39.0	36.0	41.0	32.0	41.0
34	37.081159047479055	39.0	35.0	41.0	31.0	41.0
35	37.02055159488461	39.0	35.0	41.0	31.0	41.0
36	36.84296450095546	39.0	35.0	41.0	31.0	41.0
37	36.69927789210642	39.0	35.0	41.0	30.0	41.0
38	36.62908826988094	39.0	35.0	40.0	30.0	41.0
39	36.561902836983684	39.0	35.0	40.0	30.0	41.0
40	36.365280023519034	39.0	35.0	40.0	30.0	41.0
41	36.3427807584889	39.0	35.0	40.0	30.0	41.0
42	36.24410186682346	39.0	35.0	40.0	30.0	41.0
43	36.18574525944436	39.0	35.0	40.0	30.0	41.0
44	35.94861641922681	38.0	35.0	40.0	29.0	41.0
45	35.88970858444804	38.0	35.0	40.0	29.0	41.0
46	35.75514478906365	38.0	35.0	40.0	28.0	41.0
47	35.540607085109514	38.0	35.0	40.0	27.0	41.0
48	35.429626635307955	38.0	34.0	40.0	26.0	41.0
49	35.21625569601646	38.0	34.0	40.0	26.0	41.0
50	34.94789982360723	38.0	34.0	40.0	24.0	41.0
51	32.489839041599296	36.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	4.0
12	1.0
13	2.0
14	2.0
15	7.0
16	7.0
17	9.0
18	24.0
19	52.0
20	81.0
21	141.0
22	236.0
23	350.0
24	526.0
25	762.0
26	974.0
27	1237.0
28	1416.0
29	1678.0
30	2201.0
31	2737.0
32	3638.0
33	4846.0
34	7405.0
35	9774.0
36	10765.0
37	16502.0
38	20766.0
39	22689.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.409966191386154	22.346758782889903	29.677164486256064	10.566110539467882
2	29.461267088049393	27.989489930912832	26.740959870645302	15.808283110392473
3	28.853079523739527	27.08180214611201	28.876047332059386	15.189070998089077
4	26.17687049830957	26.149309128325736	30.38549169484051	17.28832867852418
5	25.46762457739233	29.666139938262532	26.562729678083198	18.303505806261946
6	24.69223136851389	35.507312950169045	25.61002498897545	14.190430692341613
7	61.91018668234602	26.82915625459356	7.593157430545347	3.6674996325150673
8	83.28862266647067	5.531566955754814	7.187086579450243	3.9927237983242687
9	77.13416874908128	7.591320005879759	8.447559900044098	6.826951344994855
10	32.40849625165369	40.28921064236366	15.164265765103629	12.138027340879024
11	25.992209319417903	26.76760252829634	29.569675143319124	17.670513008966633
12	26.850286638247834	22.87593708657945	31.71394972806115	18.55982654711157
13	22.58746141408202	27.378546229604588	32.69145965015434	17.342532706159048
14	19.395854769954433	31.25	29.067139497280614	20.287005732764957
15	16.989747170366016	27.496141408202263	37.19406879317948	18.320042628252242
16	19.35359400264589	28.21457445244745	31.421799206232542	21.010032338674115
17	19.193738056739672	26.717992062325447	30.072210789357634	24.016059091577247
18	20.404600911362632	26.739122445979714	32.707077759811845	20.149198882845802
19	20.161142143172132	29.597236513302956	28.786932235778334	21.45468910774658
20	21.423452888431573	29.04141555196237	30.78237542260767	18.752756136998382
21	21.151514037924446	29.62112303395561	30.596795531383215	18.630567396736733
22	20.241988828458034	25.840621784506833	28.87237248272821	25.045016904306923
23	17.184514184918417	29.466779362046157	30.34690577686315	23.001800676172277
24	19.96270027928855	26.913677789210645	31.451198000881963	21.672423930618844
25	17.52260032338674	31.913310304277527	28.62156401587535	21.942525356460386
26	17.148684403939438	30.308319858885785	29.8011906511833	22.741805085991473
27	18.60024988975452	30.027193885050714	30.75665147728943	20.615904747905336
28	16.601131853594005	28.10524768484492	33.32261502278406	21.97100543877701
29	18.28145671027488	25.031236219315005	33.99603116272233	22.691275907687785
30	19.16985153608702	27.616492723798324	32.28538879905924	20.92826694105542
31	21.99581067176246	29.09194473026606	27.877407026311925	21.034837571659562
32	21.75327061590475	28.868697633397026	28.88155960605615	20.49647214464207
33	21.349037189475233	27.38589592826694	27.851683080993677	23.41338380126415
34	20.546082610612963	27.337204174628837	30.051080405703367	22.06563280905483
35	19.899309128325736	25.960973100102898	31.04328972512127	23.096428046450097
36	23.779950022049096	25.697302660590914	29.97023372041746	20.552513596942525
37	19.828568278700573	28.170476260473322	33.245443186829334	18.755512273996764
38	21.65864324562693	27.646810230780538	28.062068205203587	22.632478318388944
39	21.051374393649862	27.483279435543146	29.84528884315743	21.620057327649565
40	21.98754226076731	26.64725121270028	29.345509334117303	22.019697192415112
41	21.617301190651183	28.108922534176102	27.753380861384684	22.520395413788037
42	23.342642951638982	24.882404821402325	30.63630016169337	21.138652065265322
43	23.666029692782594	24.893429369395857	29.557731882992798	21.88280905482875
44	20.82904600911363	26.04549463471998	29.250881963839483	23.874577392326916
45	19.600727620167575	26.511281787446716	28.707004262825226	25.18098632956049
46	23.414302513596944	27.834227546670586	28.249485521093636	20.501984418638834
47	19.82764956636778	26.35785682787006	32.6510363075114	21.16345729825077
48	21.830442451859476	24.96600764368661	30.754814052623843	22.448735851830076
49	21.404159929442894	23.417058650595326	32.11083345582831	23.06794796413347
50	20.49279729531089	24.499301778627075	31.17650301337645	23.83139791268558
51	20.552513596942525	24.376194326032632	28.410260179332646	26.661031897692194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	105.0
2	154.0
3	405.0
4	656.0
5	465.0
6	274.0
7	261.0
8	248.0
9	252.5
10	257.0
11	265.0
12	273.0
13	317.5
14	362.0
15	333.5
16	305.0
17	316.0
18	327.0
19	325.5
20	324.0
21	366.5
22	409.0
23	473.5
24	538.0
25	674.0
26	1038.0
27	1266.0
28	1438.5
29	1611.0
30	1886.0
31	2161.0
32	2549.0
33	2937.0
34	3460.5
35	3984.0
36	4385.5
37	4787.0
38	5201.0
39	5615.0
40	6162.0
41	6709.0
42	7604.5
43	8500.0
44	9248.0
45	9996.0
46	12283.5
47	14571.0
48	13349.5
49	12128.0
50	11014.5
51	9901.0
52	8123.5
53	6346.0
54	5213.0
55	4080.0
56	3510.0
57	2940.0
58	2484.5
59	2029.0
60	1775.0
61	1521.0
62	1289.0
63	1057.0
64	896.0
65	735.0
66	598.0
67	461.0
68	356.0
69	251.0
70	177.0
71	103.0
72	94.5
73	86.0
74	63.0
75	31.0
76	22.0
77	13.5
78	5.0
79	5.0
80	5.0
81	5.0
82	5.0
83	2.5
84	0.0
85	0.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	108848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84808172864913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49568505769417	43.48632956048802
2	10.149002876628204	11.539026899897104
3	4.552506545137206	7.764037924445098
4	2.711787711302886	6.166397177715713
5	1.7679950871068877	5.025356460385124
6	1.1247939493842722	3.8365427017492286
7	0.8435954620382041	3.3569748640305748
8	0.6367368046801771	2.8957812729678083
9	0.4347263971039788	2.2242025576951345
>10	1.2476162771906008	9.693333823313244
>50	0.011312582824267106	0.4492503307364398
>100	0.019392999127315038	1.832831103924739
>500	0.0048482497818287595	1.7299353226517715
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	781	0.7175143319123916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	576	0.5291783036895487	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	526	0.483242687049831	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.3105247684844921	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	249	0.22875937086579448	Illumina Single End Adapter 1 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	198	0.18190504189328238	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	174	0.15985594590621785	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	168	0.1543436719094517	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	167	0.15342495957665736	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	141	0.1295384389240041	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTT	133	0.12218874026164928	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	110	0.1010583566073791	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.187123327943555E-4	0.0	0.0	0.12310745259444363	0.0
2	9.187123327943555E-4	0.0	0.0	0.9545421137733353	0.0
3	9.187123327943555E-4	0.0	0.0	1.2816037042481259	0.0
4	9.187123327943555E-4	0.0	0.0	1.7841393502866383	0.0
5	9.187123327943555E-4	0.0	0.0	3.53520505659268	0.0
6	9.187123327943555E-4	0.0	0.0	4.225158018521241	0.0
7	0.0027561369983830665	0.0	0.0	4.998713802734088	0.0
8	0.0027561369983830665	0.0	0.0	6.345546082610613	0.0
9	0.0027561369983830665	0.0	0.0	6.8196016463325	0.0
10	0.0027561369983830665	0.0	0.0	8.9519329707482	0.0
11	0.0027561369983830665	0.0	0.0	10.824268704983096	0.0
12	0.0027561369983830665	0.0	0.0	12.909745700426283	0.0
13	0.0027561369983830665	0.0	0.0	13.542738497721594	0.0
14	0.0027561369983830665	0.0	0.0	13.763229457592239	0.0
15	0.0027561369983830665	0.0	0.0	14.240959870645304	0.0
16	0.0027561369983830665	0.0	0.0	15.116492723798324	0.0
17	0.0027561369983830665	0.0	0.0	16.24007790680582	0.0
18	0.0027561369983830665	0.0	0.0	17.41051741878583	0.0
19	0.0027561369983830665	0.0	0.0	18.103226517712773	0.0
20	0.0027561369983830665	0.0	0.0	18.725194767014553	0.0
21	0.0027561369983830665	0.0	0.0	19.568572688519772	0.0
22	0.0027561369983830665	0.0	0.0	20.47350433632221	0.0
23	0.0027561369983830665	0.0	0.0	21.317800970160224	0.0
24	0.0027561369983830665	0.0	0.0	21.924151109804498	0.0
25	0.0027561369983830665	0.0	0.0	22.487321769807437	0.0
26	0.0027561369983830665	0.0	0.0	23.027524621490517	0.0
27	0.0027561369983830665	0.0	0.0	23.562215199176833	0.0
28	0.0027561369983830665	0.0	0.0	24.07853153020726	0.0
29	0.0027561369983830665	0.0	0.0	24.63251506688226	0.0
30	0.0027561369983830665	0.0	0.0	25.296744083492577	0.0
31	0.0027561369983830665	0.0	0.0	25.926980743789503	0.0
32	0.0027561369983830665	0.0	0.0	26.454321622813463	0.0
33	0.0027561369983830665	0.0	0.0	26.96604439217992	0.0
34	0.0027561369983830665	0.0	0.0	27.505328531530207	0.0
35	0.0027561369983830665	0.0	0.0	28.108003821843305	0.0
36	0.0027561369983830665	0.0	0.0	28.664743495516685	0.0
37	0.0027561369983830665	0.0	0.0	29.209539908863736	0.0
38	0.0027561369983830665	0.0	0.0	29.77546670586506	0.0
39	0.0027561369983830665	0.0	0.0	30.371711009848596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	20	7.005133E-4	45.0	3
CAGGTAT	20	7.005133E-4	45.0	30
TTAGGAA	20	7.005133E-4	45.0	5
ACGTTTT	20	7.005133E-4	45.0	29
CGACGGG	25	3.8674876E-5	45.0	3
GACGTTT	20	7.005133E-4	45.0	28
TTTCGAC	20	7.005133E-4	45.0	24
TATGGGC	25	3.8674876E-5	45.0	4
ACTCTTA	20	7.005133E-4	45.0	7
GGACTAA	55	1.8189894E-12	45.0	8
GGATCGT	20	7.005133E-4	45.0	8
GTTAGCG	20	7.005133E-4	45.0	1
GCCCTAC	40	6.717528E-9	45.0	26
CGGGAGC	25	3.8674876E-5	45.0	6
AAGGGTG	20	7.005133E-4	45.0	6
CTTTCGA	20	7.005133E-4	45.0	23
TCTTTCG	20	7.005133E-4	45.0	22
GGCGATT	35	1.198423E-7	45.0	8
AGTAGGC	20	7.005133E-4	45.0	18
TCGACGT	20	7.005133E-4	45.0	26
>>END_MODULE