##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223093
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994258000026896	33.0	31.0	34.0	30.0	34.0
2	32.12718910947452	33.0	31.0	34.0	30.0	34.0
3	31.33423729117453	33.0	31.0	34.0	28.0	34.0
4	35.42056003550089	37.0	35.0	37.0	33.0	37.0
5	35.750749687350115	37.0	35.0	37.0	35.0	37.0
6	35.822580717458635	37.0	35.0	37.0	35.0	37.0
7	26.987144374767475	36.0	11.0	37.0	0.0	37.0
8	31.32704298207474	37.0	21.0	37.0	17.0	37.0
9	36.16340718893018	39.0	32.0	39.0	32.0	39.0
10	36.93288449211764	38.0	37.0	39.0	34.0	39.0
11	37.405431815431236	39.0	37.0	39.0	35.0	39.0
12	37.46543818048975	39.0	37.0	39.0	35.0	39.0
13	37.47182565118583	39.0	37.0	39.0	35.0	39.0
14	38.699219607966185	40.0	38.0	41.0	35.0	41.0
15	38.788074928393094	40.0	38.0	41.0	35.0	41.0
16	38.72569287247919	40.0	38.0	41.0	35.0	41.0
17	38.656694741654825	40.0	38.0	41.0	35.0	41.0
18	38.42972661625421	40.0	38.0	41.0	34.0	41.0
19	38.17750893125289	40.0	37.0	41.0	34.0	41.0
20	38.03175805605734	40.0	36.0	41.0	34.0	41.0
21	37.97821536309969	40.0	36.0	41.0	34.0	41.0
22	37.941235269596085	40.0	36.0	41.0	34.0	41.0
23	37.86139412711291	40.0	35.0	41.0	34.0	41.0
24	37.77793117668416	40.0	35.0	41.0	33.0	41.0
25	37.674261406677935	39.0	35.0	41.0	33.0	41.0
26	37.59893407681998	39.0	35.0	41.0	33.0	41.0
27	37.49473089698019	39.0	35.0	41.0	33.0	41.0
28	37.4252845225982	39.0	35.0	41.0	33.0	41.0
29	37.399788429040804	39.0	35.0	41.0	33.0	41.0
30	37.25179185362158	39.0	35.0	41.0	33.0	41.0
31	37.05701209809362	39.0	35.0	41.0	32.0	41.0
32	36.77391491440789	39.0	35.0	41.0	31.0	41.0
33	36.4261317029221	39.0	35.0	41.0	30.0	41.0
34	35.96803575190616	39.0	35.0	41.0	27.0	41.0
35	35.600211570959196	39.0	35.0	41.0	24.0	41.0
36	35.28180175980421	39.0	35.0	41.0	23.0	41.0
37	35.08716544221468	39.0	35.0	41.0	22.0	41.0
38	34.86809536829932	39.0	35.0	41.0	20.0	41.0
39	34.73400330803746	39.0	34.0	41.0	19.0	41.0
40	34.55700537444026	39.0	34.0	40.0	18.0	41.0
41	34.43288225089985	38.0	34.0	40.0	18.0	41.0
42	34.32574307575764	38.0	34.0	40.0	17.0	41.0
43	34.26993227039844	38.0	34.0	40.0	17.0	41.0
44	34.0362091145845	38.0	33.0	40.0	15.0	41.0
45	33.94242311502378	38.0	33.0	40.0	15.0	41.0
46	33.86693889992066	38.0	33.0	40.0	15.0	41.0
47	33.72968672257758	38.0	33.0	40.0	15.0	41.0
48	33.63392396892775	38.0	33.0	40.0	15.0	41.0
49	33.52252199755259	38.0	33.0	40.0	15.0	41.0
50	33.322340907155315	37.0	33.0	40.0	13.0	41.0
51	31.5171161802477	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	19.0
11	6.0
12	11.0
13	7.0
14	10.0
15	13.0
16	19.0
17	48.0
18	89.0
19	149.0
20	262.0
21	499.0
22	812.0
23	1329.0
24	2382.0
25	3904.0
26	5097.0
27	5572.0
28	5185.0
29	4677.0
30	4737.0
31	5257.0
32	6451.0
33	8525.0
34	12171.0
35	16060.0
36	18876.0
37	31512.0
38	41939.0
39	47455.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.119434495927706	19.66085892430511	24.210979277700332	21.008727302066852
2	39.29482323515305	22.17371230831985	23.927241105727205	14.604223350799892
3	29.359504780517543	21.004693110048276	35.18532629889777	14.450475810536412
4	25.14780831312502	23.400554925524332	35.13467477688677	16.31696198446388
5	23.019996145105402	27.333443900077548	33.34394176419699	16.302618190620056
6	24.11102096435119	31.10496519388775	31.993832168647156	12.790181673113905
7	56.738221279914654	26.82423921862183	13.42534279426069	3.012196707202826
8	78.51658277041413	3.7128013877620547	15.494435056232152	2.276180785591659
9	71.38637249936124	7.651069285006701	17.26903130084763	3.6935269147844174
10	28.250998462524596	38.77351597764161	22.379904344824805	10.595581215008988
11	28.229931015316485	22.526479988166372	33.44390007754613	15.799688918971011
12	26.036675287884425	20.940146037751074	35.2458391791764	17.777339495188105
13	23.929930567072923	20.63937461058841	37.21138717933776	18.2193076430009
14	19.741542764676616	23.137884200759324	37.25621153509971	19.86436149946435
15	19.081728247860756	23.691913237976987	38.72824337832205	18.49811513584021
16	23.355730569762386	24.33245328181521	35.60533051238721	16.706485636035197
17	23.101576472592146	23.3629024666843	35.25390756321355	18.28161349751001
18	23.08319848672975	22.977413007131556	36.082709901251945	17.856678604886753
19	22.277256570129946	24.36158911306047	34.777872905021674	18.583281411787908
20	22.339562424639052	25.036643910835394	35.04726728315097	17.57652638137458
21	23.053166168369245	23.709394736724146	36.23107851882399	17.00636057608262
22	23.11681675355121	22.042825189494963	36.42292676148512	18.417431295468706
23	20.24357554921042	23.89138162111765	36.605361889436246	19.259680940235686
24	19.513835037405926	24.120434079061198	36.693217626729655	19.672513256803217
25	20.88232261881816	25.322623300596614	35.01320077277189	18.781853307813336
26	20.236403652288505	25.207872949846028	34.75232302223736	19.8034003756281
27	19.81236524678049	24.161224242804572	36.24407758199496	19.782332928419986
28	18.820850497326227	24.991371311515824	36.68470099913489	19.503077192023056
29	20.342637375444323	24.138363821365978	36.039678519720475	19.479320283469225
30	21.49820926698731	23.388452349468608	36.139636833069616	18.973701550474466
31	21.679747907823195	23.803077640266615	35.389725361172246	19.127449090737944
32	21.66854181888271	24.507716512844418	35.190705221589205	18.633036446683672
33	21.773879054923285	24.37951885536525	34.873797026352236	18.97280506335923
34	20.43587203542917	24.934444379698153	35.47354690644709	19.15613667842559
35	20.955834562267754	25.800002689461344	33.73705136422927	19.507111384041632
36	21.89580130259578	26.50643453626963	32.41025043367564	19.187513727458953
37	21.348944162300025	27.413231253333812	31.86563451116799	19.372190073198173
38	21.107340884743135	27.406059356411898	32.1238228003568	19.362776958488165
39	21.275432218850433	26.78165608064798	31.3985647241285	20.54434697637308
40	22.201503408892258	26.14290901104024	32.247089778702154	19.40849780136535
41	20.56362144935072	26.289932897939423	31.970075260093324	21.17637039261653
42	21.22881488885801	26.25990057957892	32.03955301152434	20.471731520038727
43	21.179508097519868	26.43561205416575	32.28115628908124	20.103723559233146
44	21.32966968932239	26.778518375744643	31.46624950132904	20.425562433603922
45	21.17861161040463	26.02995163452013	31.363153483076566	21.428283271998673
46	20.85811746670671	27.129044837803068	31.104068706772516	20.90876898871771
47	20.672992877409868	26.80451650208657	32.18568041130829	20.33681020919527
48	20.526865477625922	26.52750198347774	32.52948322000242	20.416149318893915
49	20.958972267171088	26.274244373422746	32.0108654238367	20.75591793556947
50	20.084897329813128	26.42306123455241	32.962038253105206	20.530003182529256
51	20.297813019682376	25.137946954857394	32.74239890987167	21.821841115588565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	201.0
2	318.0
3	4817.0
4	9316.0
5	6398.5
6	3481.0
7	2992.5
8	2504.0
9	2423.5
10	2343.0
11	2231.0
12	2119.0
13	1961.0
14	1803.0
15	1695.0
16	1587.0
17	1534.0
18	1481.0
19	1392.0
20	1303.0
21	1337.5
22	1372.0
23	1485.5
24	1599.0
25	1802.5
26	2237.5
27	2469.0
28	2837.0
29	3205.0
30	3797.0
31	4389.0
32	5023.5
33	5658.0
34	6353.5
35	7049.0
36	7726.5
37	8404.0
38	8884.5
39	9365.0
40	10242.0
41	11119.0
42	11574.5
43	12030.0
44	12889.5
45	13749.0
46	14634.5
47	15520.0
48	15768.0
49	16016.0
50	15647.5
51	15279.0
52	14403.0
53	13527.0
54	12607.0
55	11687.0
56	10898.0
57	10109.0
58	9176.5
59	8244.0
60	7573.5
61	6903.0
62	6260.5
63	5618.0
64	4866.0
65	4114.0
66	3380.0
67	2646.0
68	2179.0
69	1712.0
70	1448.0
71	1184.0
72	921.5
73	659.0
74	576.5
75	385.5
76	277.0
77	219.0
78	161.0
79	125.5
80	90.0
81	65.0
82	40.0
83	33.0
84	26.0
85	19.0
86	12.0
87	9.0
88	6.0
89	6.0
90	6.0
91	6.0
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	223093.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.37512487478651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.08393751197234	32.92320292145593
2	16.281299951502927	16.729076362646367
3	8.018532147315868	12.35859271142528
4	4.391895372915245	9.025366928820711
5	2.7880829082549883	7.161905378642898
6	1.5967278972390888	4.921925706702809
7	1.0331926817853758	3.715628213250747
8	0.6542728715010919	2.689068038044299
9	0.4250157931296987	1.9651715501215232
>10	0.7053988681713004	4.6611538673737645
>50	0.014757270144609674	0.49431810091166306
>100	0.004919090048203225	0.5393020589700864
>500	9.838180096406449E-4	0.41951331672462194
>1k	9.838180096406449E-4	2.3957748449092873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4740	2.124674463116279	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	830	0.37204215282415853	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.19633067823732706	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04706557355004415	0.0
2	0.0	0.0	0.0	0.14298969488061034	0.0
3	0.0	0.0	0.0	0.25460234072785787	0.0
4	0.0	0.0	0.0	0.3527676798465214	0.0
5	0.0	0.0	0.0	0.6566768119125208	0.0
6	0.0	0.0	0.0	1.0430627585805023	0.0
7	0.0	0.0	0.0	1.3353175581483956	0.0
8	0.0	0.0	0.0	1.890243082481297	0.0
9	0.0	0.0	0.0	2.284697413186429	0.0
10	0.0	0.0	0.0	2.8118318369469235	0.0
11	0.0	0.0	0.0	3.2600753945663916	0.0
12	0.0	0.0	0.0	3.6191184842195856	0.0
13	0.0	0.0	0.0	3.8212763287059657	0.0
14	0.0	0.0	0.0	3.9727826511813458	0.0
15	0.0	0.0	0.0	4.088429489047169	0.0
16	0.0	0.0	0.0	4.246211221329221	0.0
17	0.0	0.0	0.0	4.455540962737513	0.0
18	0.0	0.0	0.0	4.69445477894869	0.0
19	0.0	0.0	0.0	4.831169064022627	0.0
20	0.0	0.0	0.0	4.991640257650397	0.0
21	0.0	0.0	0.0	5.1570421304119805	0.0
22	0.0	0.0	0.0	5.350235103745971	0.0
23	0.0	0.0	0.0	5.543428077079962	0.0
24	0.0	0.0	0.0	5.705692244938209	0.0
25	0.0	0.0	0.0	5.852716131837395	0.0
26	0.0	0.0	0.0	5.993016365372289	0.0
27	0.0	0.0	0.0	6.171865544862457	0.0
28	0.0	0.0	0.0	6.33726741762404	0.0
29	0.0	0.0	0.0	6.515220109998969	0.0
30	0.0	0.0	0.0	6.745169055057756	0.0
31	0.0	0.0	0.0	6.930741887912216	0.0
32	0.0	0.0	0.0	7.133796219513835	0.0
33	0.0	0.0	0.0	7.322506757271631	0.0
34	0.0	0.0	0.0	7.529595280891825	0.0
35	0.0	0.0	0.0	7.719202305764861	0.0
36	0.0	0.0	0.0	7.911498791983612	0.0
37	0.0	0.0	0.0	8.098416355510931	0.0
38	0.0	0.0	0.0	8.330606518357815	0.0
39	0.0	0.0	0.0	8.668582160802893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCG	30	2.1576125E-6	45.000004	30
GTTAGCG	20	7.02105E-4	45.000004	1
CACGACG	40	6.7720975E-9	45.000004	26
TAACGAA	20	7.02105E-4	45.000004	1
CTGCGAC	20	7.02105E-4	45.000004	44
TGCGACA	20	7.02105E-4	45.000004	45
CGAGGGC	20	7.02105E-4	45.000004	4
GCGGGTA	20	7.02105E-4	45.000004	5
TCCGTGG	20	7.02105E-4	45.000004	36
CCGGTCG	30	2.1576125E-6	45.000004	16
CGCACGG	20	7.02105E-4	45.000004	2
TACACGG	20	7.02105E-4	45.000004	2
TAATCGT	20	7.02105E-4	45.000004	21
GCGGGCG	35	1.2060445E-7	45.0	5
CGTTTTA	420	0.0	43.92857	1
CGTTTTT	2040	0.0	43.89706	1
CGGTTTT	120	0.0	43.125004	1
ACAACGA	50	1.0732037E-9	40.5	13
CAACGAG	50	1.0732037E-9	40.5	14
GGATTAC	50	1.0732037E-9	40.5	8
>>END_MODULE