##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116796
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78790369533203	31.0	31.0	34.0	30.0	34.0
2	31.907471146272133	33.0	31.0	34.0	30.0	34.0
3	31.785086818041712	33.0	31.0	34.0	30.0	34.0
4	35.57508818795164	37.0	35.0	37.0	33.0	37.0
5	35.663943970683924	37.0	35.0	37.0	35.0	37.0
6	35.64640056166307	37.0	35.0	37.0	33.0	37.0
7	26.988655433405253	35.0	18.0	37.0	0.0	37.0
8	31.173028185896776	35.0	23.0	37.0	17.0	37.0
9	35.84925853625124	39.0	32.0	39.0	32.0	39.0
10	36.49550498304736	37.0	35.0	39.0	32.0	39.0
11	36.85783759717798	39.0	37.0	39.0	33.0	39.0
12	36.93802013767595	39.0	37.0	39.0	33.0	39.0
13	36.86758108154389	39.0	37.0	39.0	33.0	39.0
14	38.06557587588616	40.0	37.0	41.0	33.0	41.0
15	38.10071406555019	40.0	37.0	41.0	33.0	41.0
16	38.08496010137333	40.0	37.0	41.0	33.0	41.0
17	37.912642556251924	40.0	37.0	41.0	33.0	41.0
18	37.81991677797185	40.0	36.0	41.0	33.0	41.0
19	37.69294325148122	39.0	36.0	41.0	33.0	41.0
20	37.7999674646392	39.0	36.0	41.0	33.0	41.0
21	37.810378780095206	39.0	36.0	41.0	33.0	41.0
22	37.8617760882222	39.0	36.0	41.0	33.0	41.0
23	37.85627076269735	39.0	36.0	41.0	33.0	41.0
24	37.78810061988424	39.0	36.0	41.0	33.0	41.0
25	37.735881365800196	39.0	36.0	41.0	33.0	41.0
26	37.545986163909724	39.0	36.0	41.0	33.0	41.0
27	37.4977396486181	39.0	36.0	41.0	33.0	41.0
28	37.486754683379566	39.0	36.0	41.0	33.0	41.0
29	37.48967430391452	39.0	36.0	41.0	33.0	41.0
30	37.44298606116648	39.0	36.0	41.0	33.0	41.0
31	37.32857289633207	39.0	36.0	41.0	32.0	41.0
32	37.216959484913865	39.0	36.0	41.0	32.0	41.0
33	37.15134936127949	39.0	36.0	41.0	32.0	41.0
34	37.03058323915202	39.0	35.0	41.0	31.0	41.0
35	37.02151614781328	39.0	35.0	41.0	31.0	41.0
36	36.804530977088255	39.0	35.0	41.0	31.0	41.0
37	36.752928182472004	39.0	35.0	41.0	30.0	41.0
38	36.60473817596493	39.0	35.0	41.0	30.0	41.0
39	36.57816192335354	39.0	35.0	40.0	30.0	41.0
40	36.429227028322885	39.0	35.0	40.0	30.0	41.0
41	36.291080173978564	39.0	35.0	40.0	30.0	41.0
42	36.226394739545874	39.0	35.0	40.0	30.0	41.0
43	36.127307442035686	39.0	35.0	40.0	29.0	41.0
44	35.930280146580365	39.0	35.0	40.0	29.0	41.0
45	35.840842152128495	38.0	35.0	40.0	28.0	41.0
46	35.57019075995753	38.0	35.0	40.0	27.0	41.0
47	35.34193808007124	38.0	34.0	40.0	26.0	41.0
48	35.17206068700983	38.0	34.0	40.0	24.0	41.0
49	34.9553837460187	38.0	34.0	40.0	24.0	41.0
50	34.75897291003117	38.0	34.0	40.0	23.0	41.0
51	32.281559299976024	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	5.0
11	5.0
12	5.0
13	2.0
14	3.0
15	2.0
16	3.0
17	17.0
18	29.0
19	44.0
20	116.0
21	176.0
22	274.0
23	382.0
24	597.0
25	880.0
26	1147.0
27	1420.0
28	1655.0
29	2013.0
30	2488.0
31	3309.0
32	4224.0
33	5535.0
34	7933.0
35	10144.0
36	11097.0
37	16991.0
38	22007.0
39	24282.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08705777595123	20.838042398712282	27.72183978903387	9.353060036302614
2	27.976129319497247	29.464193979245863	26.119901366485156	16.43977533477174
3	28.03263810404466	28.391383266550225	28.77410185280318	14.801876776601938
4	25.53597725949519	25.670399671221617	32.612418233501145	16.181204835782047
5	27.042021987054348	29.03010377067708	25.799684920716466	18.128189321552107
6	24.31932600431522	36.76752628514675	25.744888523579572	13.168259186958458
7	60.567142710366795	26.48549607863283	9.037980752765504	3.9093804582348715
8	81.50107880406863	6.691153806637214	7.498544470701052	4.309222918593102
9	76.1687044076852	6.488235898489675	8.701496626596802	8.64156306722833
10	35.281174012808656	35.84112469605124	15.91664098085551	12.9610603102846
11	25.896434809411282	25.561663070653108	29.70564060413028	18.836261515805337
12	25.835645056337547	23.32870988732491	30.655159423267918	20.180485633069626
13	21.10175005993356	26.76033425802253	34.80341792527141	17.334497756772492
14	18.742936401931573	31.0395903969314	28.60885646768725	21.608616733449775
15	15.572451111339431	26.891331894927912	38.980787013253874	18.55542998047878
16	19.018630775026544	27.020617144422754	31.06356382067879	22.897188259871914
17	19.284907017363608	25.88444809753759	31.794753244974146	23.03589164012466
18	19.498099249974317	26.20980170553786	33.695503270659955	20.59659577382787
19	19.553751840816467	28.536936196445083	29.618308846193365	22.291003116545088
20	20.317476625911844	28.46501592520292	32.751121613753895	18.46638583513134
21	19.507517380732217	30.49162642556252	31.85211822322682	18.14873797047844
22	19.002363094626528	24.85701565122093	31.038734203226138	25.101887050926404
23	16.087879721908287	29.96249871570944	31.733107298195147	22.21651426418713
24	18.873934038836946	25.620740436316314	32.18089660604815	23.32442891879859
25	16.16408096167677	32.54991609301688	28.967601630192814	22.31840131511353
26	16.47916024521388	30.209082502825442	29.72790164046714	23.583855611493547
27	19.409911298332137	29.392273708003696	29.53354566937224	21.664269324291926
28	15.069009212644268	28.388814685434433	34.42155553272372	22.120620569197573
29	18.602520634268295	26.668721531559296	31.791328470153086	22.937429364019316
30	20.886845439912328	28.98044453577177	30.650022261036337	19.482687763279564
31	19.724990581869243	30.774170348299602	26.749203739854106	22.751635329977056
32	20.877427309154424	29.666255693688143	28.649953765539916	20.80636323161752
33	21.04010411315456	26.698688311243536	28.5934449809925	23.667762594609403
34	17.96551251755197	28.068598239665743	29.693653892256584	24.272235350525705
35	18.983526833110723	25.925545395390255	28.585739237645125	26.5051885338539
36	20.743004897427994	29.280112332614134	29.04894003219288	20.927942737764994
37	18.037432788794135	27.957293057981435	33.576492345628274	20.42878180759615
38	18.878215007363266	28.602863111750406	29.722764478235558	22.796157402650778
39	19.41247987944793	25.357888968800303	32.57388951676427	22.6557416349875
40	20.68478372547005	25.386999554779273	29.636288914003906	24.29192780574677
41	17.20178773245659	25.833076475221755	29.662830918867083	27.30230487345457
42	18.04171375732046	25.315079283537106	32.027637932805916	24.615569026336516
43	19.73184013151135	26.630192814822422	31.01304839206822	22.624918661598002
44	19.72927155039556	26.11476420425357	30.19367101613069	23.96229322922018
45	18.567416692352477	26.055686838590365	28.913661426761188	26.463235042295967
46	22.047844104250146	28.03349429774992	28.066885852255215	21.851775745744717
47	16.396965649508545	25.99917805404295	34.71009281139765	22.893763485050858
48	19.277201274016235	25.90242816534813	30.938559539710266	23.881811020925376
49	18.743792595636837	23.31158601321963	33.936949895544366	24.007671495599165
50	19.293468954416245	23.281619233535395	32.18774615569026	25.237165656358094
51	18.769478406794754	23.37323195999863	29.084043974108702	28.773245659097913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	110.0
2	155.0
3	410.5
4	666.0
5	461.5
6	257.0
7	238.0
8	219.0
9	227.5
10	236.0
11	247.5
12	259.0
13	245.5
14	232.0
15	237.5
16	243.0
17	284.5
18	326.0
19	359.0
20	392.0
21	444.5
22	497.0
23	627.0
24	757.0
25	883.0
26	1279.0
27	1549.0
28	1781.0
29	2013.0
30	2573.0
31	3133.0
32	3465.0
33	3797.0
34	4198.5
35	4600.0
36	4931.5
37	5263.0
38	5648.0
39	6033.0
40	6501.5
41	6970.0
42	7776.5
43	8583.0
44	9557.5
45	10532.0
46	13427.0
47	16322.0
48	14317.5
49	12313.0
50	11286.5
51	10260.0
52	8437.5
53	6615.0
54	5497.5
55	4380.0
56	3673.5
57	2967.0
58	2487.5
59	2008.0
60	1737.0
61	1466.0
62	1220.5
63	975.0
64	828.5
65	682.0
66	551.5
67	421.0
68	332.5
69	244.0
70	199.0
71	154.0
72	125.0
73	96.0
74	66.5
75	28.5
76	20.0
77	22.5
78	25.0
79	16.5
80	8.0
81	5.0
82	2.0
83	3.5
84	5.0
85	4.0
86	3.0
87	2.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	116796.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.5412171649714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35206447692455	40.87982465152916
2	9.697124764128315	10.383917257440324
3	4.2648799053315	6.850405835816295
4	2.5841941983560943	5.534436110825713
5	1.7750343812965745	4.751875064214528
6	1.31928231042313	4.238158841056201
7	0.9674736943102952	3.625980341792527
8	0.7435954840566732	3.1850405835816296
9	0.5469024850481339	2.635364224802219
>10	1.707870918220488	12.199904106305011
>50	0.014392170659161416	0.46063221343196686
>100	0.0223878210253622	2.326278297201959
>500	0.0015991300732401573	0.7551628480427411
>1k	0.0031982601464803145	2.1730196239597244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG	1363	1.1669920202746669	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC	1175	1.0060276036850577	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC	882	0.7551628480427411	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.303948765368677	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC	304	0.2602828864002192	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	290	0.2482961745265249	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG	287	0.24572759341073325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCT	247	0.2114798452001781	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTT	166	0.14212815507380389	No Hit
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	158	0.13527860543169287	No Hit
GAATCTATCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC	152	0.1301414432001096	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTC	151	0.1292852494948457	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGCCTTCGTA	142	0.12157950614747082	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	126	0.10788040686324873	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	117	0.10017466351587383	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2268913318949279	0.0
2	0.0	0.0	0.0	1.1318880783588479	0.0
3	0.0	0.0	0.0	1.406726257748553	0.0
4	0.0	0.0	0.0	1.8716394397068392	0.0
5	0.0	0.0	0.0	3.7980752765505668	0.0
6	0.0	0.0	0.0	4.3640193157299905	0.0
7	0.0	0.0	0.0	5.144011781225385	0.0
8	0.0	0.0	0.0	6.50279119147916	0.0
9	0.0	0.0	0.0	6.9171889448268775	0.0
10	0.0	0.0	0.0	9.663002157608137	0.0
11	0.0	0.0	0.0	11.38309531148327	0.0
12	0.0	0.0	0.0	14.110928456453989	0.0
13	0.0	0.0	0.0	14.752217541696634	0.0
14	0.0	0.0	0.0	14.982533648412616	0.0
15	0.0	0.0	0.0	15.503955614918318	0.0
16	0.0	0.0	0.0	16.44405630329806	0.0
17	0.0	0.0	0.0	17.59049967464639	0.0
18	0.0	0.0	0.0	18.766053631973698	0.0
19	0.0	0.0	0.0	19.468988663995344	0.0
20	0.0	0.0	0.0	20.201890475701223	0.0
21	0.0	0.0	0.0	21.041816500565087	0.0
22	0.0	0.0	0.0	22.02215829309223	0.0
23	0.0	0.0	0.0	22.985376211514094	0.0
24	0.0	0.0	0.0	23.656632076440975	0.0
25	0.0	0.0	0.0	24.27651631905202	0.0
26	0.0	0.0	0.0	24.860440426041986	0.0
27	0.0	0.0	0.0	25.45463885749512	0.0
28	0.0	0.0	0.0	26.05054967635878	0.0
29	0.0	0.0	0.0	26.645604301517174	0.0
30	0.0	0.0	0.0	27.35025172094935	0.0
31	0.0	0.0	0.0	28.0197951984657	0.0
32	0.0	0.0	0.0	28.62854892290832	0.0
33	0.0	0.0	0.0	29.19620534949827	0.0
34	0.0	0.0	0.0	29.756156032740847	0.0
35	0.0	0.0	0.0	30.405150861330867	0.0
36	0.0	0.0	0.0	30.978800643857667	0.0
37	0.0	0.0	0.0	31.591835336826605	0.0
38	0.0	0.0	0.0	32.1963080927429	0.0
39	0.0	0.0	0.0	32.814479947943425	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTCTC	30	2.148725E-6	45.000004	7
AAACGGC	30	2.148725E-6	45.000004	6
AGGGGTC	30	2.148725E-6	45.000004	6
AAGGACC	30	2.148725E-6	45.000004	6
TCACGAC	20	7.007247E-4	45.0	25
AAATAAG	20	7.007247E-4	45.0	2
CAACGCA	25	3.869237E-5	45.0	17
CGACGGT	20	7.007247E-4	45.0	28
TTATGGG	40	6.724804E-9	45.0	3
AGGACTA	25	3.869237E-5	45.0	7
GTCTCAC	25	3.869237E-5	45.0	22
GTTAGGA	35	1.1994234E-7	45.0	4
ATCAACG	25	3.869237E-5	45.0	15
ATCCGGG	20	7.007247E-4	45.0	3
GGCTTAG	35	1.1994234E-7	45.0	1
GTTGTCA	20	7.007247E-4	45.0	34
CAAGGAT	45	3.783498E-10	45.0	5
CGGGCTC	20	7.007247E-4	45.0	6
AACGCAA	25	3.869237E-5	45.0	18
GACGGTC	20	7.007247E-4	45.0	29
>>END_MODULE