##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936376.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 221294 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.997650184822003 33.0 31.0 34.0 30.0 34.0 2 32.12878794725569 33.0 31.0 34.0 30.0 34.0 3 31.851808002024455 33.0 31.0 34.0 30.0 34.0 4 35.67788552784983 37.0 35.0 37.0 33.0 37.0 5 35.828947011667736 37.0 35.0 37.0 35.0 37.0 6 35.834636275723696 37.0 35.0 37.0 35.0 37.0 7 26.927729626650518 36.0 11.0 37.0 0.0 37.0 8 31.265321246848085 37.0 19.0 37.0 17.0 37.0 9 36.06468770052509 39.0 32.0 39.0 32.0 39.0 10 36.73769736188058 38.0 35.0 39.0 33.0 39.0 11 37.24013303569008 39.0 37.0 39.0 34.0 39.0 12 37.2990456135277 39.0 37.0 39.0 34.0 39.0 13 37.29494247471689 39.0 37.0 39.0 34.0 39.0 14 38.530262908167416 40.0 38.0 41.0 34.0 41.0 15 38.61078474789195 40.0 38.0 41.0 35.0 41.0 16 38.54032192467938 40.0 38.0 41.0 34.0 41.0 17 38.45535351161803 40.0 38.0 41.0 34.0 41.0 18 38.3176317478106 40.0 38.0 41.0 34.0 41.0 19 38.19269388234656 40.0 37.0 41.0 34.0 41.0 20 38.214990013285494 40.0 37.0 41.0 34.0 41.0 21 38.172806311965076 40.0 37.0 41.0 34.0 41.0 22 38.16702215152693 40.0 37.0 41.0 34.0 41.0 23 38.08680307645033 40.0 37.0 41.0 34.0 41.0 24 38.07230652435222 40.0 37.0 41.0 34.0 41.0 25 37.98353773712798 40.0 37.0 41.0 33.0 41.0 26 37.8926812294956 40.0 37.0 41.0 33.0 41.0 27 37.80267878930292 40.0 36.0 41.0 33.0 41.0 28 37.75270906576771 40.0 36.0 41.0 33.0 41.0 29 37.75704266722098 40.0 36.0 41.0 33.0 41.0 30 37.67543177853896 40.0 36.0 41.0 33.0 41.0 31 37.550539101828335 40.0 36.0 41.0 33.0 41.0 32 37.392514031107936 40.0 36.0 41.0 32.0 41.0 33 37.23500411217656 40.0 36.0 41.0 32.0 41.0 34 37.059102370602005 40.0 36.0 41.0 31.0 41.0 35 36.91327826330583 40.0 36.0 41.0 31.0 41.0 36 36.7294007067521 40.0 35.0 41.0 30.0 41.0 37 36.63989986172241 39.0 35.0 41.0 30.0 41.0 38 36.488860972281216 39.0 35.0 41.0 30.0 41.0 39 36.4240422243712 39.0 35.0 41.0 30.0 41.0 40 36.22344482905095 39.0 35.0 41.0 29.0 41.0 41 36.154789555975306 39.0 35.0 41.0 29.0 41.0 42 36.05365260693919 39.0 35.0 41.0 28.0 41.0 43 35.984536408578634 39.0 35.0 41.0 28.0 41.0 44 35.77010673583558 39.0 35.0 40.0 26.0 41.0 45 35.63365929487469 39.0 35.0 40.0 26.0 41.0 46 35.448105235568974 39.0 35.0 40.0 25.0 41.0 47 35.342029155783706 39.0 35.0 40.0 24.0 41.0 48 35.272944589550555 38.0 35.0 40.0 24.0 41.0 49 35.11857528898208 38.0 34.0 40.0 24.0 41.0 50 34.90280802913771 38.0 34.0 40.0 23.0 41.0 51 32.85789492711054 36.0 31.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 13.0 9 18.0 10 6.0 11 7.0 12 5.0 13 6.0 14 10.0 15 4.0 16 14.0 17 25.0 18 60.0 19 92.0 20 212.0 21 304.0 22 508.0 23 888.0 24 1274.0 25 1937.0 26 2647.0 27 2973.0 28 3293.0 29 3613.0 30 4290.0 31 5162.0 32 6496.0 33 8680.0 34 12886.0 35 16801.0 36 19910.0 37 32345.0 38 44272.0 39 52531.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.33497519137437 21.55548727032816 26.785633591511743 13.323903946785723 2 31.995896861189188 24.78151237719956 26.83127423246902 16.391316529142227 3 31.25570508011966 23.87864108380706 29.26197727909478 15.603676556978499 4 27.716521912026536 25.9265953889396 29.11195061773026 17.244932081303606 5 24.347700344338303 30.77580051876689 27.0147405713666 17.86175856552821 6 25.880954747982322 35.53824324202193 25.383426572794566 13.19737543720119 7 62.140862382170326 27.321120319574867 7.123555089609298 3.414462208645512 8 84.92503185807116 4.665738790929713 7.6753097689047145 2.7339195820944084 9 77.35455999710791 9.294422804052527 9.030972371596157 4.320044827243396 10 29.36681518703625 44.405632326226645 14.933527343714697 11.294025143022404 11 30.68451923685233 25.969524704691498 26.89499037479552 16.45096568366065 12 29.97867090838432 22.76564208699739 28.906341789655393 18.349345214962902 13 25.16290545609009 24.502245881045127 30.649723896716587 19.685124766148203 14 20.497618552694604 28.383959800084956 29.419234141006985 21.699187506213455 15 19.247245745478867 27.66455484559003 33.793505472358035 19.294693936573065 16 23.76747675038636 26.685766446446806 31.182499299574324 18.36425750359251 17 22.355780093450342 26.20540999755981 29.92399251674243 21.51481739224742 18 22.422659448516452 26.62747295453108 30.903684690954115 20.046182905998357 19 22.79908176453044 27.781141829421497 29.202328124576354 20.217448281471707 20 24.218460509548382 27.02739342232505 30.010754923314686 18.74339114481188 21 23.85830614476669 26.58996628918995 29.97460392057625 19.577123645467115 22 22.713675020560885 25.170587544172008 30.051424801395427 22.064312633871683 23 20.89392392021474 27.379865699024826 30.85985160013376 20.86635878062668 24 20.371090043110073 26.092890001536418 31.2444078917639 22.291612063589614 25 20.423057109546576 28.80285954431661 29.350999123338184 21.42308422279863 26 20.344880566124704 29.041456162390304 29.106076079785264 21.507587191699727 27 20.83924552857285 28.694406536101297 29.414715265664682 21.051632669661174 28 18.94131788480483 28.18964816036585 31.39307889052573 21.475955064303594 29 20.405433495711588 26.750838251375995 30.655146547127348 22.188581705785065 30 20.44610337379233 27.395229875188665 30.52274349959782 21.635923251421186 31 21.353041654992904 28.24703787721312 28.609903567200195 21.79001690059378 32 22.13616275181433 28.07080173886323 29.22898948909595 20.56404602022649 33 21.08733178486538 28.24613410214466 28.63475738158287 22.03177673140709 34 20.082785796271025 28.130450893381653 30.231727927553393 21.555035382793932 35 20.51207895378998 26.21489963577865 29.80740553291097 23.465615877520403 36 19.82340235162273 29.3853425759397 28.802407656782382 21.98884741565519 37 20.084593346407946 30.023859661807368 28.557484613229462 21.334062378555224 38 19.932759134906505 29.24661310293094 27.964608168319067 22.856019593843484 39 21.390096432799805 27.539381998608185 28.447224054877225 22.62329751371479 40 20.658038627346425 27.714262474355383 29.86886223756631 21.758836660731877 41 18.5657993438593 27.70296528599962 29.954720869070105 23.776514501070974 42 19.4108290328703 27.6848897846304 29.276889567724385 23.627391614774915 43 20.90476922103627 27.48741493217168 28.60176959158405 23.006046255208005 44 20.51885726680344 28.28364076748579 28.206819886666608 22.990682079044166 45 20.369734380507378 27.557457499977406 28.243874664473505 23.82893345504171 46 19.94721953600188 29.159850696358692 28.260142615705803 22.632787151933627 47 19.579383083138268 27.922582627635634 29.85394994893671 22.64408434028939 48 19.924625159290354 26.84528274603017 29.933030267427043 23.29706182725243 49 19.648973763409764 26.608041790559167 30.122371144269618 23.62061330176146 50 19.25266839588963 26.53754733521921 30.17885708604842 24.030927182842735 51 19.363832729310328 26.115032490713713 29.397543539363923 25.123591240612036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 94.0 1 234.5 2 375.0 3 1687.0 4 2999.0 5 2063.0 6 1127.0 7 949.0 8 771.0 9 774.5 10 778.0 11 771.5 12 765.0 13 723.0 14 681.0 15 674.5 16 668.0 17 655.5 18 643.0 19 676.5 20 710.0 21 852.0 22 994.0 23 1104.5 24 1215.0 25 1525.0 26 2218.0 27 2601.0 28 3200.5 29 3800.0 30 4560.5 31 5321.0 32 5959.5 33 6598.0 34 7384.0 35 8170.0 36 8931.5 37 9693.0 38 10322.0 39 10951.0 40 11948.5 41 12946.0 42 13733.5 43 14521.0 44 15254.0 45 15987.0 46 17428.0 47 18869.0 48 19023.0 49 19177.0 50 18172.5 51 17168.0 52 15243.0 53 13318.0 54 11718.5 55 10119.0 56 9643.5 57 9168.0 58 8744.0 59 8320.0 60 7793.5 61 7267.0 62 6288.5 63 5310.0 64 4298.5 65 3287.0 66 2661.0 67 2035.0 68 1599.0 69 1163.0 70 982.5 71 802.0 72 637.5 73 473.0 74 356.5 75 187.5 76 135.0 77 114.0 78 93.0 79 72.5 80 52.0 81 40.0 82 28.0 83 20.5 84 13.0 85 9.5 86 6.0 87 5.5 88 5.0 89 2.5 90 0.0 91 0.5 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 221294.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.06850064573962 #Duplication Level Percentage of deduplicated Percentage of total 1 68.32965794505047 32.161745491097754 2 10.92246266840787 10.282078823220456 3 5.985089675091132 8.451275917105095 4 4.006249035652858 7.542725412864812 5 2.7176338456319242 6.395747520900503 6 2.0636428976802836 5.827954623722426 7 1.5005553474235789 4.944022323742233 8 1.1950527260904973 4.4999472007746775 9 0.8855570033347332 3.751365634497012 >10 2.371136272788128 14.348985238322886 >50 0.0149755975099101 0.48119734344740867 >100 0.0069886121712913795 0.5606137018874595 >500 0.0 0.0 >1k 9.983731673273398E-4 0.7523407684172865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1601 0.7234719423029996 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.10709734561262392 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 233 0.10528979547570201 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07365766807956836 0.0 2 0.0 0.0 0.0 0.3172250490297975 0.0 3 0.0 0.0 0.0 0.43923468327202725 0.0 4 0.0 0.0 0.0 0.6109519462796099 0.0 5 0.0 0.0 0.0 1.1545726499588782 0.0 6 0.0 0.0 0.0 1.5183421150144152 0.0 7 0.0 0.0 0.0 1.888438005549179 0.0 8 0.0 0.0 0.0 2.643542075248312 0.0 9 0.0 0.0 0.0 2.9756794129077155 0.0 10 0.0 0.0 0.0 3.812575126302566 0.0 11 0.0 0.0 0.0 4.682458629696241 0.0 12 0.0 0.0 0.0 5.503538279393025 0.0 13 0.0 0.0 0.0 5.791390638697841 0.0 14 0.0 0.0 0.0 5.915207823076993 0.0 15 0.0 0.0 0.0 6.11900910101494 0.0 16 0.0 0.0 0.0 6.564118322231963 0.0 17 0.0 0.0 0.0 7.089211637007781 0.0 18 0.0 0.0 0.0 7.664012580548953 0.0 19 0.0 0.0 0.0 8.023263170262185 0.0 20 0.0 0.0 0.0 8.448941227507298 0.0 21 0.0 0.0 0.0 8.951440165571592 0.0 22 0.0 0.0 0.0 9.488282556237404 0.0 23 0.0 0.0 0.0 9.994848482109772 0.0 24 0.0 0.0 0.0 10.359973609768002 0.0 25 0.0 0.0 0.0 10.728713837700074 0.0 26 0.0 0.0 0.0 11.051813424674867 0.0 27 0.0 0.0 0.0 11.371749798910047 0.0 28 0.0 0.0 0.0 11.72196263793867 0.0 29 0.0 0.0 0.0 12.086635878062667 0.0 30 0.0 0.0 0.0 12.535812087087766 0.0 31 0.0 0.0 0.0 12.941607092826738 0.0 32 0.0 0.0 0.0 13.327067159525336 0.0 33 0.0 0.0 0.0 13.673213010745885 0.0 34 0.0 0.0 0.0 14.057769302376025 0.0 35 0.0 0.0 0.0 14.473053946333836 0.0 36 0.0 0.0 0.0 14.846313049608213 0.0 37 0.0 0.0 0.0 15.243974079731036 0.0 38 0.0 0.0 0.0 15.628530371361176 0.0 39 0.0 0.0 0.0 16.02799895162092 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAT 20 7.020927E-4 45.000004 43 GTCTCGC 20 7.020927E-4 45.000004 31 TCTCGCG 20 7.020927E-4 45.000004 32 CCGAGGG 35 1.20599E-7 45.000004 3 AGTCTCG 20 7.020927E-4 45.000004 30 ATGCGCG 20 7.020927E-4 45.000004 1 AGTACGG 20 7.020927E-4 45.000004 2 CTCACGT 85 0.0 45.0 45 CGACGGT 95 0.0 45.0 28 CGTAAAG 25 3.880565E-5 45.0 2 ACGGTCT 95 0.0 45.0 30 CGTTTTT 645 0.0 42.55814 1 TACGAAT 65 0.0 41.53846 12 CGAATAT 65 0.0 41.53846 14 CGACCAC 60 3.6379788E-12 41.249996 20 ATAGGAC 55 6.002665E-11 40.909092 5 TAGGGTA 110 0.0 40.909092 5 CGGTCTA 105 0.0 40.714287 31 CGTTTTA 105 0.0 40.714287 1 CAACGCA 75 0.0 39.0 17 >>END_MODULE