##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936375.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230171
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980349392408254	33.0	31.0	34.0	30.0	34.0
2	32.115392469077335	33.0	31.0	34.0	30.0	34.0
3	31.840453402035877	33.0	31.0	34.0	30.0	34.0
4	35.669271976052585	37.0	35.0	37.0	33.0	37.0
5	35.7948785902655	37.0	35.0	37.0	35.0	37.0
6	35.79179827171972	37.0	35.0	37.0	35.0	37.0
7	26.906469537865327	35.0	11.0	37.0	0.0	37.0
8	31.22006247529011	36.0	19.0	37.0	17.0	37.0
9	36.03467856506684	39.0	32.0	39.0	32.0	39.0
10	36.737660261284	38.0	35.0	39.0	33.0	39.0
11	37.20044662446616	39.0	37.0	39.0	34.0	39.0
12	37.226644538191174	39.0	37.0	39.0	34.0	39.0
13	37.161510355344504	39.0	37.0	39.0	34.0	39.0
14	38.27485651971795	40.0	38.0	41.0	34.0	41.0
15	38.34576467061446	40.0	38.0	41.0	34.0	41.0
16	38.32661803615573	40.0	38.0	41.0	34.0	41.0
17	38.24899748447893	40.0	37.0	41.0	33.0	41.0
18	38.11218615724831	40.0	37.0	41.0	33.0	41.0
19	37.980918534480885	40.0	37.0	41.0	33.0	41.0
20	38.00304556177798	40.0	37.0	41.0	33.0	41.0
21	37.96064230506884	40.0	37.0	41.0	33.0	41.0
22	37.985801860356	40.0	37.0	41.0	33.0	41.0
23	37.93992727146339	40.0	36.0	41.0	33.0	41.0
24	37.87106542527078	40.0	36.0	41.0	33.0	41.0
25	37.76025216035035	40.0	36.0	41.0	33.0	41.0
26	37.668498637969165	40.0	36.0	41.0	33.0	41.0
27	37.557628893301064	40.0	36.0	41.0	33.0	41.0
28	37.51939210413128	40.0	36.0	41.0	33.0	41.0
29	37.487311607457066	40.0	36.0	41.0	33.0	41.0
30	37.36498950780072	39.0	36.0	41.0	32.0	41.0
31	37.226105808290356	39.0	35.0	41.0	32.0	41.0
32	37.06554257486825	39.0	35.0	41.0	31.0	41.0
33	36.888535045683426	39.0	35.0	41.0	31.0	41.0
34	36.69622150488115	39.0	35.0	41.0	30.0	41.0
35	36.524201571874826	39.0	35.0	41.0	30.0	41.0
36	36.313575559040885	39.0	35.0	41.0	30.0	41.0
37	36.209218363738266	39.0	35.0	41.0	29.0	41.0
38	36.001151317933186	39.0	35.0	41.0	28.0	41.0
39	35.942025711318976	39.0	35.0	40.0	28.0	41.0
40	35.722662715980725	39.0	35.0	40.0	27.0	41.0
41	35.66053933814425	39.0	35.0	40.0	26.0	41.0
42	35.58736765274513	39.0	35.0	40.0	26.0	41.0
43	35.548257599784506	38.0	35.0	40.0	26.0	41.0
44	35.344126757932145	38.0	34.0	40.0	26.0	41.0
45	35.24723792310934	38.0	34.0	40.0	25.0	41.0
46	35.13187152160785	38.0	34.0	40.0	24.0	41.0
47	34.97600045183798	38.0	34.0	40.0	24.0	41.0
48	34.884199138901074	38.0	34.0	40.0	24.0	41.0
49	34.73972828896777	38.0	34.0	40.0	24.0	41.0
50	34.54679347094117	38.0	34.0	40.0	23.0	41.0
51	32.34547792728015	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	16.0
11	16.0
12	6.0
13	3.0
14	7.0
15	14.0
16	25.0
17	46.0
18	82.0
19	119.0
20	260.0
21	483.0
22	706.0
23	1040.0
24	1554.0
25	2266.0
26	2934.0
27	3456.0
28	3657.0
29	4035.0
30	4727.0
31	5846.0
32	7292.0
33	9918.0
34	14554.0
35	18420.0
36	21829.0
37	34504.0
38	44277.0
39	48051.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.71630657207033	22.0027718522316	27.640319588479866	12.640601987218197
2	32.22299942216873	24.291939471088885	27.022518040934784	16.462543065807594
3	31.14076056497126	24.45008276455331	28.918499724118156	15.490656946357273
4	27.292317450938654	25.100903241503058	30.003779798497636	17.602999509060655
5	25.49495809637183	29.509364776622597	26.794426752284174	18.201250374721404
6	25.72348384461987	34.59558328373253	25.742165607309346	13.938767264338253
7	61.449096541267146	27.376602612840017	7.643447697581364	3.5308531483114725
8	84.27256257304352	4.46016222721368	8.197383684304278	3.069891515438522
9	77.54973476241577	7.730339617067311	9.665857123616789	5.054068496900131
10	32.70959417129004	39.873398473308974	15.556260345569164	11.86074700983182
11	31.68079384457642	24.217212420330974	26.13795830056784	17.964035434524767
12	30.08372036442471	21.436670996780656	28.67780910714208	19.801799531652552
13	26.896959217277587	22.524123369147286	31.083846357707966	19.49507105586716
14	22.08531917574325	25.292065464372143	30.936130094581856	21.686485265302753
15	21.22899930920924	24.473978042411947	33.75707626069314	20.539946387685674
16	25.25948099456491	25.153472852792053	29.42247285713665	20.164573295506386
17	25.624861516003318	23.541193286730298	29.136163982430453	21.697781214835928
18	24.861950462916703	23.648939266892878	30.590300255027785	20.89881001516264
19	24.660361209709304	25.497130394358976	28.47752323272697	21.364985163204746
20	24.445303708981584	26.669736847821834	29.25650929091849	19.62845015227809
21	25.031389705914297	25.232544499524266	30.47864413848834	19.25742165607309
22	24.108597520973536	22.258668555117715	30.668937442162562	22.96379648174618
23	22.39943346468495	25.085262695995585	31.041269317159852	21.474034522159613
24	22.654895707973637	24.709455144218865	29.70487159546598	22.930777552341517
25	22.68443896059886	26.869153803042085	28.535740818782557	21.910666417576497
26	21.858531265884928	25.96895351716767	28.840731456178233	23.331783760769166
27	21.65737647227496	25.925507557424698	30.115435915037082	22.30168005526326
28	20.454792306589447	25.337683722102263	31.59216408670076	22.615359884607532
29	23.27877968988274	24.838489644655496	29.96685073271611	21.915879932745653
30	23.719756181273922	25.700023026358664	29.113137623766676	21.467083168600738
31	24.768107189871877	24.670788240047617	28.714303713326178	21.846800856754324
32	25.188664080183866	26.01804745167723	27.684200007820277	21.10908846031863
33	23.783621742096095	26.16402587641362	27.44307493124677	22.609277450243514
34	23.369147286148127	25.42935469715994	29.68836213076365	21.51313588592829
35	22.76090384974649	26.63454562043003	27.87927236706623	22.725278162757252
36	24.59866794687428	26.518544907916286	27.848860195246143	21.033926949963288
37	23.2474985988678	27.862328442766465	28.02220957462061	20.867963383745128
38	22.93164647153638	27.81931694262092	27.265815415495435	21.983221170347264
39	23.434750685360015	26.20616845736431	28.074779185909605	22.284301671366073
40	23.24880197766009	25.399811444534713	29.09402140147977	22.25736517632543
41	21.384535845089086	25.872503486538267	28.880701739141767	23.862258929230876
42	22.290384105730087	25.633116248354483	28.84377267336024	23.232726972555188
43	22.86647753192192	25.81732711766469	28.71039357694931	22.605801773464076
44	22.833893062114687	25.613131106872718	28.54486447032858	23.008111360684012
45	22.23781449444109	25.2329789591217	28.109535953703986	24.41967059273323
46	22.66966733428625	26.44946583192496	28.64565909693228	22.23520773685651
47	21.628267679247166	25.130880953725708	30.595948229794374	22.644903137232752
48	21.686485265302753	25.89987444117634	29.170920750224834	23.24271954329607
49	22.263447610689443	24.649934179370987	30.612023234899272	22.474594975040297
50	21.745571770553198	24.841965321434934	29.441589079423558	23.97087382858831
51	21.522259537474312	25.345069535258567	28.360219141420945	24.772451785846176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	168.5
2	276.0
3	1770.5
4	3265.0
5	2155.0
6	1045.0
7	917.5
8	790.0
9	751.0
10	712.0
11	717.0
12	722.0
13	715.5
14	709.0
15	677.0
16	645.0
17	628.0
18	611.0
19	637.5
20	664.0
21	737.5
22	811.0
23	883.0
24	955.0
25	1226.5
26	1800.0
27	2102.0
28	2417.5
29	2733.0
30	3218.0
31	3703.0
32	4335.0
33	4967.0
34	5499.5
35	6032.0
36	6742.5
37	7453.0
38	8347.0
39	9241.0
40	10122.0
41	11003.0
42	11763.5
43	12524.0
44	13848.0
45	15172.0
46	17993.0
47	20814.0
48	20336.5
49	19859.0
50	19473.0
51	19087.0
52	17539.5
53	15992.0
54	15026.0
55	14060.0
56	13069.0
57	12078.0
58	11561.5
59	11045.0
60	9891.0
61	8737.0
62	7622.0
63	6507.0
64	5647.5
65	4788.0
66	4045.5
67	3303.0
68	2840.5
69	2378.0
70	1997.0
71	1616.0
72	1293.5
73	971.0
74	757.0
75	434.0
76	325.0
77	253.5
78	182.0
79	140.5
80	99.0
81	71.0
82	43.0
83	35.0
84	27.0
85	20.0
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	230171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.74632798924098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.8958170370277	30.602379872841233
2	11.403890177350634	10.433722008127312
3	6.276837127977434	8.614267499745033
4	4.154922006069571	7.602896994374948
5	2.8875834923870856	6.604817076952877
6	2.1553439518465924	5.915944281048061
7	1.6386864676999315	5.247472203605227
8	1.2068218455920305	4.4166134378427335
9	0.9155886484499833	3.7696336753696915
>10	2.4355854220351905	14.051945615940237
>50	0.01595797162142836	0.47177452272957177
>100	0.008976359037053454	0.6811986097052715
>500	0.0029921196790178178	0.7432501910314047
>1k	9.973732263392726E-4	0.8440840106863712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	0.8037502552450134	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	572	0.2485108897298096	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	540	0.23460818261205799	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	517	0.22461561187117404	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.1303378792289211	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	239	0.10383584378570715	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08776083868080688	0.0
2	0.0	0.0	0.0	0.38884133969961465	0.0
3	0.0	0.0	0.0	0.5526326079306255	0.0
4	0.0	0.0	0.0	0.7663867298660562	0.0
5	0.0	0.0	0.0	1.5292977829526744	0.0
6	0.0	0.0	0.0	1.8807755972733315	0.0
7	0.0	0.0	0.0	2.284388563285557	0.0
8	0.0	0.0	0.0	3.0468651567747456	0.0
9	0.0	0.0	0.0	3.3592416073267266	0.0
10	0.0	0.0	0.0	4.388041934040344	0.0
11	0.0	0.0	0.0	5.186578674116201	0.0
12	0.0	0.0	0.0	6.204517510894075	0.0
13	0.0	0.0	0.0	6.5112459866794685	0.0
14	0.0	0.0	0.0	6.656355492221001	0.0
15	0.0	0.0	0.0	6.907473139535389	0.0
16	0.0	0.0	0.0	7.31803745910649	0.0
17	0.0	0.0	0.0	7.827224107294142	0.0
18	0.0	0.0	0.0	8.368560765691594	0.0
19	0.0	0.0	0.0	8.686150731412733	0.0
20	0.0	0.0	0.0	9.055875848825439	0.0
21	0.0	0.0	0.0	9.512058426126663	0.0
22	0.0	0.0	0.0	9.981274791350778	0.0
23	0.0	0.0	0.0	10.405307358442201	0.0
24	0.0	0.0	0.0	10.710732455435307	0.0
25	0.0	0.0	0.0	11.02050214840271	0.0
26	0.0	0.0	0.0	11.33287859895469	0.0
27	0.0	0.0	0.0	11.670888165755025	0.0
28	0.0	0.0	0.0	11.979788939527568	0.0
29	0.0	0.0	0.0	12.335176890225094	0.0
30	0.0	0.0	0.0	12.728362825899005	0.0
31	0.0	0.0	0.0	13.049428468399581	0.0
32	0.0	0.0	0.0	13.36354275734128	0.0
33	0.0	0.0	0.0	13.675919207893262	0.0
34	0.0	0.0	0.0	14.013059855498739	0.0
35	0.0	0.0	0.0	14.368882265793692	0.0
36	0.0	0.0	0.0	14.708195211386316	0.0
37	0.0	0.0	0.0	15.046639237784083	0.0
38	0.0	0.0	0.0	15.419840031976227	0.0
39	0.0	0.0	0.0	15.922509786202433	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	35	1.206281E-7	45.0	33
CGACGGT	35	1.206281E-7	45.0	28
CGAATAG	35	1.206281E-7	45.0	26
TCGTAAC	35	1.206281E-7	45.0	34
AGTAGCG	20	7.0215197E-4	45.0	1
TGTACGG	45	3.8198777E-10	45.0	2
TAAGCGG	20	7.0215197E-4	45.0	2
CGTTTTA	115	0.0	43.043476	1
CGTTTTT	720	0.0	42.5	1
GCTCGTA	45	1.916851E-8	40.0	32
TCACGAC	40	3.4421828E-7	39.375	25
CTCACGA	40	3.4421828E-7	39.375	24
CACGACG	40	3.4421828E-7	39.375	26
CGGGGTC	40	3.4421828E-7	39.375	6
CGTAACC	40	3.4421828E-7	39.375	35
CGGTCTA	40	3.4421828E-7	39.375	31
TTAGGAT	30	1.1370875E-4	37.499996	5
CGGTTTA	30	1.1370875E-4	37.499996	1
ATGCGAG	30	1.1370875E-4	37.499996	1
ATACGGG	60	1.546141E-10	37.499996	3
>>END_MODULE