##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248952
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.939112760692826	33.0	31.0	34.0	30.0	34.0
2	32.07286545197468	33.0	31.0	34.0	30.0	34.0
3	31.241231241363796	33.0	31.0	34.0	27.0	34.0
4	35.35551833285131	37.0	35.0	37.0	33.0	37.0
5	35.69101272534464	37.0	35.0	37.0	35.0	37.0
6	35.76406696873293	37.0	35.0	37.0	35.0	37.0
7	27.087715704232142	35.0	17.0	37.0	0.0	37.0
8	31.35830601883094	37.0	25.0	37.0	17.0	37.0
9	36.16307159613098	39.0	32.0	39.0	32.0	39.0
10	36.92520646550339	38.0	37.0	39.0	34.0	39.0
11	37.37011954111636	39.0	37.0	39.0	35.0	39.0
12	37.39249333204795	39.0	37.0	39.0	35.0	39.0
13	37.36023811819146	39.0	37.0	39.0	35.0	39.0
14	38.55315482502651	40.0	38.0	41.0	35.0	41.0
15	38.647116713261994	40.0	38.0	41.0	35.0	41.0
16	38.587426491853854	40.0	38.0	41.0	34.0	41.0
17	38.510905716764675	40.0	38.0	41.0	34.0	41.0
18	38.26278961406215	40.0	38.0	41.0	34.0	41.0
19	38.011134676564154	40.0	37.0	41.0	34.0	41.0
20	37.87431713744015	40.0	35.0	41.0	34.0	41.0
21	37.809537581541825	40.0	35.0	41.0	33.0	41.0
22	37.79249815225425	39.0	35.0	41.0	33.0	41.0
23	37.70832128281757	39.0	35.0	41.0	33.0	41.0
24	37.64305167261159	39.0	35.0	41.0	33.0	41.0
25	37.52183553456088	39.0	35.0	41.0	33.0	41.0
26	37.4307938879784	39.0	35.0	41.0	33.0	41.0
27	37.32456055785854	39.0	35.0	41.0	33.0	41.0
28	37.27396445901218	39.0	35.0	41.0	33.0	41.0
29	37.227999775057036	39.0	35.0	41.0	33.0	41.0
30	37.06172274173335	39.0	35.0	41.0	33.0	41.0
31	36.85593206722581	39.0	35.0	41.0	32.0	41.0
32	36.53796715832771	39.0	35.0	41.0	31.0	41.0
33	36.17894212538963	39.0	35.0	41.0	30.0	41.0
34	35.67698592499759	39.0	35.0	41.0	25.0	41.0
35	35.28474163694206	39.0	35.0	41.0	23.0	41.0
36	34.98625437835406	39.0	34.0	41.0	22.0	41.0
37	34.81635415662457	39.0	34.0	41.0	21.0	41.0
38	34.60148944374819	38.0	34.0	40.0	18.0	41.0
39	34.48603746907034	38.0	34.0	40.0	18.0	41.0
40	34.29646678877856	38.0	34.0	40.0	18.0	41.0
41	34.16894019730711	38.0	34.0	40.0	16.0	41.0
42	34.079754330152	38.0	33.0	40.0	15.0	41.0
43	34.045936566085025	38.0	33.0	40.0	15.0	41.0
44	33.85709695041614	38.0	33.0	40.0	15.0	41.0
45	33.774217519843184	38.0	33.0	40.0	15.0	41.0
46	33.70694752402069	38.0	33.0	40.0	15.0	41.0
47	33.61914344933963	38.0	33.0	40.0	15.0	41.0
48	33.55207429544651	38.0	33.0	40.0	15.0	41.0
49	33.46903820816864	37.0	33.0	40.0	15.0	41.0
50	33.292494135415666	37.0	33.0	40.0	15.0	41.0
51	31.577906584401813	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	24.0
11	17.0
12	7.0
13	9.0
14	7.0
15	12.0
16	30.0
17	57.0
18	89.0
19	213.0
20	354.0
21	539.0
22	994.0
23	1649.0
24	2692.0
25	4434.0
26	5957.0
27	6509.0
28	6104.0
29	5674.0
30	5491.0
31	6176.0
32	7456.0
33	10023.0
34	14609.0
35	18635.0
36	20866.0
37	34269.0
38	44665.0
39	51363.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.89628522767441	19.480060413252353	23.5736206176291	22.050033741444132
2	39.62089077412514	21.763231466306756	23.921880523153057	14.693997236415052
3	29.025273948391657	21.45634499823259	35.182685818953054	14.3356952344227
4	24.79514123204473	23.444680098974903	35.75347858221665	16.006700086763715
5	22.62403997557762	27.90738776952987	33.404029692470836	16.06454256242167
6	24.105851730454063	30.96179183135705	32.392589736174045	12.539766702014846
7	56.11322664610046	26.50310099938944	14.357386162794434	3.026286191715672
8	77.28879462707671	3.8059545615219	16.480285356213244	2.4249654551881488
9	70.63851666184647	7.638420257720364	18.032793470227194	3.6902696102059833
10	29.744689739387514	36.97459751277355	22.84496609788232	10.435746649956618
11	29.517336675343035	22.202673607763746	32.75852373148238	15.521465985410842
12	27.730245187827375	20.639721713422666	34.72155274912433	16.90848034962563
13	24.609563289308785	20.530463703846525	36.49217519843182	18.367797808412867
14	19.723079147787526	22.723657572544102	38.349159677367524	19.204103602300844
15	19.63029017641955	22.983145345287443	39.20916481892092	18.17739965937209
16	24.421575243420417	23.784504643465407	35.837832192551176	15.956087920563
17	23.93152093576272	23.284006555480573	35.28993540923552	17.494537099521192
18	23.533050547896785	22.79355056396414	36.428307464892825	17.245091423246247
19	22.752177126514347	24.514765898647127	34.60667116552588	18.12638580931264
20	23.03134740833574	25.445065715479288	35.03767794594942	16.485908930235546
21	23.48645522028343	23.97048427006009	36.11017384877406	16.43288666088242
22	22.989170603168482	22.03597480638838	37.152945146052254	17.821909444390887
23	20.63249140396542	23.525418554580803	37.316430476557734	18.525659564896042
24	20.724878691474665	23.45070535685594	36.78138757672162	19.04302837494778
25	21.98496095632893	24.42117355956168	34.58176676628426	19.012098717825126
26	20.285034866158938	24.60072624441659	35.384732799897165	19.7295060895273
27	20.005864584337544	23.852790899450497	36.20577460715319	19.935569909058774
28	19.135817346315758	25.126932099360523	35.93785147337639	19.79939908094733
29	20.64494360358623	24.55292586522703	35.57553263279669	19.226597898390054
30	21.20569427038144	23.840338699829687	35.983241749413544	18.970725280375333
31	21.474822455734436	23.95722870272181	35.449002217294904	19.11894662424885
32	22.486262412031234	24.262106751502298	34.900703750120506	18.350927086345962
33	22.749365339503196	24.483434557665735	34.09171245862656	18.675487644204505
34	20.52966033612905	25.297647739323242	35.532150776053214	18.64054114849449
35	20.744962884411454	27.33257816767891	33.37792024165301	18.544538706256628
36	21.67445933352614	27.531009993894408	31.80854140557216	18.985989267007295
37	21.482052765191682	28.248819049455314	30.980670972717633	19.288457212635368
38	21.86967768887175	27.95719656801311	30.81557890677721	19.35754683633793
39	22.20428034319869	26.846942382467304	30.4050580031492	20.543719271184806
40	23.040586137086667	26.02188373662393	31.183119637520484	19.75441048876892
41	21.170747774671423	26.527603714772326	31.357852116070568	20.943796394485684
42	21.0751470162923	26.54045759825187	31.54021658793663	20.8441787975192
43	20.87591182235933	26.405491821716637	31.831839069378837	20.886757286545198
44	21.514589157749285	26.05401844532279	31.511697033966385	20.919695362961534
45	21.100854783251393	25.976091776728044	31.372714418843795	21.55033902117677
46	20.563803464121598	26.551704746296473	31.567129406471928	21.317362383109998
47	20.167743179408077	26.9063915935602	32.7520967897426	20.173768437289116
48	20.225585655066038	26.488238696616218	32.497027539445355	20.789148108872393
49	21.17757640026993	25.753157235129663	32.38415437514059	20.685111989459813
50	19.757623959638806	26.245219962081045	32.743661428709146	21.253494649571
51	19.850814614865516	25.443458980044348	32.59343166554195	22.112294739548187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	211.0
2	337.0
3	6193.5
4	12050.0
5	8067.5
6	4085.0
7	3538.0
8	2991.0
9	2822.5
10	2654.0
11	2540.5
12	2427.0
13	2285.5
14	2144.0
15	2020.0
16	1896.0
17	1789.0
18	1682.0
19	1577.5
20	1473.0
21	1421.5
22	1370.0
23	1508.5
24	1647.0
25	1815.0
26	2296.0
27	2609.0
28	3064.0
29	3519.0
30	3996.5
31	4474.0
32	5142.0
33	5810.0
34	6505.5
35	7201.0
36	7754.0
37	8307.0
38	9015.5
39	9724.0
40	10632.0
41	11540.0
42	12107.0
43	12674.0
44	13454.0
45	14234.0
46	15453.0
47	16672.0
48	17245.5
49	17819.0
50	17352.5
51	16886.0
52	16222.5
53	15559.0
54	14546.5
55	13534.0
56	12755.0
57	11976.0
58	11321.0
59	10666.0
60	9868.0
61	9070.0
62	7976.0
63	6882.0
64	5810.5
65	4739.0
66	3928.5
67	3118.0
68	2560.5
69	2003.0
70	1658.0
71	1313.0
72	1040.0
73	767.0
74	605.0
75	355.5
76	268.0
77	213.5
78	159.0
79	118.5
80	78.0
81	59.0
82	40.0
83	30.0
84	20.0
85	14.0
86	8.0
87	6.5
88	5.0
89	6.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	248952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.424128526968374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01539540657402	40.77997193220587
2	13.446162245230168	15.44268297929134
3	6.626380160855897	11.415423180766272
4	3.696235931029848	8.490125086778265
5	2.0888140071914427	5.997416200894663
6	1.3020114267185907	4.486012290688189
7	0.7485075149174918	3.0087674218016622
8	0.40501718468544745	1.860620709520642
9	0.23664891606509542	1.223042199470081
>10	0.4159829561997241	3.29732392142655
>50	0.01116696327839529	0.4588967744155693
>100	0.005583481639197645	0.3923667617055392
>500	6.979352048997056E-4	0.20520100305744235
>1k	6.979352048997056E-4	0.45569050874279676
>5k	6.979352048997056E-4	2.486459029235102
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6204	2.4920466595970305	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.456714547382628	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	512	0.20566213567274014	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.033677174716412E-4	0.0	0.0	0.03253639255760146	0.0
2	8.033677174716412E-4	0.0	0.0	0.11889842218580289	0.0
3	8.033677174716412E-4	0.0	0.0	0.21449918056492817	0.0
4	8.033677174716412E-4	0.0	0.0	0.31492014524888334	0.0
5	8.033677174716412E-4	0.0	0.0	0.5936887432115427	0.0
6	8.033677174716412E-4	0.0	0.0	0.9668530479771201	0.0
7	8.033677174716412E-4	0.0	0.0	1.2709277290401362	0.0
8	8.033677174716412E-4	0.0	0.0	1.8433272277386805	0.0
9	8.033677174716412E-4	0.0	0.0	2.260676756965198	0.0
10	8.033677174716412E-4	0.0	0.0	2.81982068832546	0.0
11	8.033677174716412E-4	0.0	0.0	3.2198978116263377	0.0
12	8.033677174716412E-4	0.0	0.0	3.5247758604068253	0.0
13	8.033677174716412E-4	0.0	0.0	3.7143706417301328	0.0
14	8.033677174716412E-4	0.0	0.0	3.85013978598284	0.0
15	8.033677174716412E-4	0.0	0.0	3.943732125068286	0.0
16	8.033677174716412E-4	0.0	0.0	4.092756836659276	0.0
17	8.033677174716412E-4	0.0	0.0	4.243789967543944	0.0
18	8.033677174716412E-4	0.0	0.0	4.44503358077059	0.0
19	8.033677174716412E-4	0.0	0.0	4.575179151000996	0.0
20	8.033677174716412E-4	0.0	0.0	4.721793759439571	0.0
21	8.033677174716412E-4	0.0	0.0	4.879655515922749	0.0
22	8.033677174716412E-4	0.0	0.0	5.073267135833413	0.0
23	8.033677174716412E-4	0.0	0.0	5.230727208457855	0.0
24	8.033677174716412E-4	0.0	0.0	5.364086249558148	0.0
25	8.033677174716412E-4	0.0	0.0	5.503068864680742	0.0
26	8.033677174716412E-4	0.0	0.0	5.6372312734985055	0.0
27	8.033677174716412E-4	0.0	0.0	5.782640830360873	0.0
28	8.033677174716412E-4	0.0	0.0	5.925640284070825	0.0
29	8.033677174716412E-4	0.0	0.0	6.0762717310967576	0.0
30	8.033677174716412E-4	0.0	0.0	6.3124618400334205	0.0
31	8.033677174716412E-4	0.0	0.0	6.475545486680163	0.0
32	8.033677174716412E-4	0.0	0.0	6.672370577460716	0.0
33	8.033677174716412E-4	0.0	0.0	6.845496320575854	0.0
34	8.033677174716412E-4	0.0	0.0	7.059593817282046	0.0
35	8.033677174716412E-4	0.0	0.0	7.237539766702015	0.0
36	8.033677174716412E-4	0.0	0.0	7.426732864166587	0.0
37	8.033677174716412E-4	0.0	0.0	7.65127414119991	0.0
38	8.033677174716412E-4	0.0	0.0	7.929641055303834	0.0
39	8.033677174716412E-4	0.0	0.0	8.345383849095407	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTC	20	7.022629E-4	45.000004	32
GCTAGCG	20	7.022629E-4	45.000004	1
TATCGCG	20	7.022629E-4	45.000004	1
TAGTCGG	20	7.022629E-4	45.000004	2
ACGGTCT	30	2.1586293E-6	45.000004	30
CGGTTTA	55	1.8189894E-12	45.0	1
CTGGTCG	25	3.881976E-5	45.0	44
TATACGG	25	3.881976E-5	45.0	2
CGTTTTT	2645	0.0	44.06427	1
CGGTTTT	155	0.0	43.54839	1
CGTTTTA	465	0.0	43.54839	1
TGCACGG	45	1.9179424E-8	40.0	2
GTACGGG	115	0.0	39.130436	3
TCACGAC	35	6.228589E-6	38.571426	25
CTCACGA	35	6.228589E-6	38.571426	24
CACGACG	35	6.228589E-6	38.571426	26
CGGTCTA	35	6.228589E-6	38.571426	31
ACAACGA	30	1.13735456E-4	37.500004	13
CGAGTCG	30	1.13735456E-4	37.500004	28
TATGGGC	30	1.13735456E-4	37.500004	4
>>END_MODULE