##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936370.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68514 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.745905946229968 31.0 31.0 34.0 30.0 34.0 2 31.86534139008086 33.0 31.0 34.0 30.0 34.0 3 31.70833698222261 31.0 31.0 34.0 30.0 34.0 4 35.52729369180022 37.0 35.0 37.0 33.0 37.0 5 35.64324079750124 37.0 35.0 37.0 35.0 37.0 6 35.64399976647108 37.0 35.0 37.0 33.0 37.0 7 27.086755991476196 35.0 19.0 37.0 0.0 37.0 8 31.298493738506 35.0 26.0 37.0 17.0 37.0 9 35.921213182707184 39.0 32.0 39.0 32.0 39.0 10 36.54638468050325 37.0 35.0 39.0 32.0 39.0 11 36.88990571270105 39.0 37.0 39.0 33.0 39.0 12 36.919359546953906 39.0 35.0 39.0 33.0 39.0 13 36.75940683655895 39.0 35.0 39.0 33.0 39.0 14 37.81428613130163 40.0 37.0 41.0 33.0 41.0 15 37.96187640482237 40.0 37.0 41.0 33.0 41.0 16 37.919957964795515 40.0 36.0 41.0 33.0 41.0 17 37.852497299821934 40.0 36.0 41.0 33.0 41.0 18 37.76108532562688 39.0 36.0 41.0 33.0 41.0 19 37.643094841930115 39.0 36.0 41.0 33.0 41.0 20 37.68336398400327 39.0 36.0 41.0 33.0 41.0 21 37.59139737863794 39.0 36.0 41.0 33.0 41.0 22 37.61324692763523 39.0 36.0 41.0 33.0 41.0 23 37.54324663572409 39.0 35.0 41.0 33.0 41.0 24 37.51515018828269 39.0 35.0 41.0 33.0 41.0 25 37.43484543305018 39.0 35.0 41.0 33.0 41.0 26 37.310637242023525 39.0 35.0 41.0 33.0 41.0 27 37.204965408529645 39.0 35.0 41.0 32.0 41.0 28 37.191552091543336 39.0 35.0 41.0 32.0 41.0 29 37.17956913915404 39.0 35.0 41.0 32.0 41.0 30 37.08646408033395 39.0 35.0 41.0 32.0 41.0 31 36.957468546574425 39.0 35.0 41.0 31.0 41.0 32 36.715123916279886 39.0 35.0 40.0 31.0 41.0 33 36.59161631199463 39.0 35.0 40.0 30.0 41.0 34 36.422950054003564 39.0 35.0 40.0 30.0 41.0 35 36.30605423709023 39.0 35.0 40.0 30.0 41.0 36 36.149210380360216 39.0 35.0 40.0 30.0 41.0 37 36.00776483638381 38.0 35.0 40.0 30.0 41.0 38 35.886271418980066 38.0 35.0 40.0 29.0 41.0 39 35.8330560177482 38.0 35.0 40.0 29.0 41.0 40 35.62537583559565 38.0 35.0 40.0 27.0 41.0 41 35.61761099921184 38.0 35.0 40.0 27.0 41.0 42 35.491622150217474 38.0 35.0 40.0 27.0 41.0 43 35.37214291969524 38.0 34.0 40.0 27.0 41.0 44 35.12679160463555 38.0 34.0 40.0 25.0 41.0 45 34.98356540269142 38.0 34.0 40.0 24.0 41.0 46 34.79388154245848 38.0 34.0 40.0 24.0 41.0 47 34.646057740023934 38.0 34.0 40.0 23.0 41.0 48 34.528359167469425 38.0 34.0 40.0 23.0 41.0 49 34.34957818839945 37.0 33.0 40.0 23.0 41.0 50 34.05892226406282 37.0 33.0 40.0 22.0 41.0 51 31.714598476223838 35.0 29.0 39.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 5.0 10 2.0 11 1.0 12 4.0 13 0.0 14 0.0 15 5.0 16 7.0 17 14.0 18 15.0 19 41.0 20 78.0 21 124.0 22 216.0 23 351.0 24 463.0 25 695.0 26 831.0 27 1003.0 28 1162.0 29 1315.0 30 1585.0 31 2020.0 32 2525.0 33 3519.0 34 5151.0 35 6598.0 36 6518.0 37 9851.0 38 12275.0 39 12137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.66050734156523 21.230697375718833 28.102285664243805 11.006509618472137 2 30.336865458154538 25.25031380447792 27.700907843652395 16.71191289371515 3 30.06538809586362 25.533467612458765 28.6262661645795 15.77487812709811 4 27.902326531803716 25.148144904691012 28.683188837317918 18.266339726187347 5 23.89000788160084 30.110634322912105 27.24260735032256 18.756750445164492 6 25.949440990162593 34.098140526023876 26.22529701958724 13.727121464226288 7 61.26047231222815 27.05286510786117 7.588230142744548 4.098432437166126 8 81.85334384213445 4.940596082552471 8.277140438450536 4.928919636862538 9 75.56995650523982 7.620340368391862 10.991914061359722 5.817789065008611 10 38.716174796391975 32.51160346790437 16.479843535627754 12.292378200075897 11 30.963014858277138 25.273666695857784 26.515748606124294 17.247569839740784 12 29.135651107802786 22.08745657821759 29.76471961934787 19.012172694631754 13 23.110605131797882 29.220305339054793 30.37335435093558 17.295735178211753 14 18.14227749073182 31.650465598271886 30.06392854015238 20.143328370843918 15 15.936888811045918 26.826633972618737 39.11025483842718 18.126222377908167 16 19.381440289575853 26.88647575677964 34.536007239396326 19.196076714248182 17 19.733193215985054 24.727792859853462 30.31643167819716 25.222582245964333 18 20.940245789181773 26.717167294275622 33.01077152114896 19.331815395393644 19 22.176489476603322 27.633768280935282 29.958840528942986 20.230901713518403 20 23.930875441515603 28.4467408120968 29.469889365677087 18.15249438071051 21 21.44379250956009 27.769506962080744 30.744081501590912 20.042619026768254 22 20.029483025367078 26.282219692325654 29.726771170855592 23.961526111451676 23 18.075137928014712 28.23072656683306 30.811221064308025 22.882914440844207 24 20.611845754152437 24.739469305543395 31.546837142773736 23.101847797530432 25 18.45900108007123 28.235105233966777 30.671103716028842 22.634789969933152 26 18.835566453571534 30.0084654231252 29.221764894766032 21.934203228537232 27 19.412090959511925 29.151706220626437 29.9909507545903 21.44525206527133 28 16.955658697492485 27.798698076305573 32.51160346790437 22.734039758297577 29 19.988615465452316 26.30995125083924 31.676737601074233 22.024695682634206 30 20.330151501882828 27.350614472954433 30.50325480923607 21.815979215926674 31 21.613100972064103 27.915462533204895 27.152114896225587 23.319321598505415 32 23.465277169629566 28.23072656683306 28.17672300551712 20.12727325802026 33 21.621858306331553 29.122515106401615 26.283679248036897 22.97194733922994 34 19.65145809615553 26.5405610532154 30.54558192486207 23.262398925767 35 18.710044662404766 27.36521003006685 29.59541115684386 24.32933415068453 36 22.97194733922994 26.578509501707682 28.5065825962577 21.942960562804682 37 19.733193215985054 27.238228683188836 32.361269229646496 20.667308871179614 38 18.36704907026301 29.24657734185714 28.76492395714744 23.621449630732407 39 20.784073328078932 26.922964649560672 31.52056513997139 20.772396882389003 40 21.770732988878187 26.65732551011472 31.38336690311469 20.1885745978924 41 20.124354146597774 26.222377908164756 28.890445748314214 24.762822196923256 42 22.76031176109992 25.4677876054529 28.46863414776542 23.30326648568176 43 22.57494818577225 24.23884169658756 28.60437282891088 24.58183728872931 44 20.17689815220247 26.288057915170622 29.392532912981288 24.14251101964562 45 18.708585106693523 26.622296173044923 29.699039612342006 24.97007910791955 46 22.433371281781824 27.445485594185133 28.59707505035467 21.524068073678375 47 19.08515048019383 26.63397261873486 32.05622208599702 22.22465481507429 48 20.281986163411858 25.41670315555945 29.799748956417666 24.50156172461103 49 20.721312432495548 23.215693143007268 32.326239892576695 23.736754531920486 50 19.829523892926993 25.008027556411825 30.557258370552002 24.605190180109172 51 18.42397174300143 24.818285313950433 29.14732755349272 27.61041538955542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 109.0 2 152.0 3 347.5 4 543.0 5 383.0 6 223.0 7 206.0 8 189.0 9 192.0 10 195.0 11 197.0 12 199.0 13 215.0 14 231.0 15 239.0 16 247.0 17 250.0 18 253.0 19 241.0 20 229.0 21 276.0 22 323.0 23 368.5 24 414.0 25 559.5 26 775.0 27 845.0 28 904.5 29 964.0 30 1195.0 31 1426.0 32 1610.5 33 1795.0 34 1944.5 35 2094.0 36 2233.5 37 2373.0 38 2667.5 39 2962.0 40 3414.0 41 3866.0 42 4358.0 43 4850.0 44 5415.0 45 5980.0 46 6553.5 47 7127.0 48 7258.0 49 7389.0 50 6926.0 51 6463.0 52 5446.0 53 4429.0 54 3798.0 55 3167.0 56 2725.5 57 2284.0 58 2184.5 59 2085.0 60 1830.5 61 1576.0 62 1342.5 63 1109.0 64 876.5 65 644.0 66 520.5 67 397.0 68 357.5 69 318.0 70 252.5 71 187.0 72 136.0 73 85.0 74 72.5 75 41.0 76 22.0 77 21.5 78 21.0 79 13.5 80 6.0 81 6.5 82 7.0 83 6.5 84 6.0 85 6.5 86 7.0 87 3.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 68514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.074875207986686 #Duplication Level Percentage of deduplicated Percentage of total 1 74.92182116923729 41.2630995125084 2 9.892934753802935 10.897042940129024 3 4.717231144326072 7.7940274980296005 4 3.0025971272592358 6.6147064833464695 5 1.939895054857688 5.3419739031438835 6 1.383367785021466 4.571328487608372 7 1.1077542799597182 4.270660011092623 8 0.8400911644670588 3.701433283708439 9 0.6439815550961997 3.1920483404851563 >10 1.523824667408703 10.762763814694805 >50 0.018550908994540732 0.8085938640277899 >100 0.007950389569088886 0.7823218612254429 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.3765653735003065 No Hit GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC 169 0.2466649151998132 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG 109 0.1590915725253233 No Hit GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC 87 0.12698134687801033 No Hit GGGTAAGGGACACTTCTGTTGTTAACTGTTCTAATTTTTTTTTGTCTATGT 86 0.12552179116676881 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC 85 0.12406223545552734 No Hit TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC 84 0.12260267974428583 No Hit CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC 78 0.11384534547683686 No Hit CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT 74 0.10800712263187084 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.19849957672884375 0.0 2 0.0 0.0 0.0 1.058177890650086 0.0 3 0.0 0.0 0.0 1.4055521499255628 0.0 4 0.0 0.0 0.0 1.872609977522842 0.0 5 0.0 0.0 0.0 3.231456344688677 0.0 6 0.0 0.0 0.0 3.841550631987623 0.0 7 0.0 0.0 0.0 4.568409376185889 0.0 8 0.0 0.0 0.0 6.156405990016639 0.0 9 0.0 0.0 0.0 6.7314709402457895 0.0 10 0.0 0.0 0.0 8.36909244825875 0.0 11 0.0 0.0 0.0 10.130776191727238 0.0 12 0.0 0.0 0.0 11.63265901859474 0.0 13 0.0 0.0 0.0 12.185830633155268 0.0 14 0.0 0.0 0.0 12.37411331990542 0.0 15 0.0 0.0 0.0 12.775491140496833 0.0 16 0.0 0.0 0.0 13.619114341594418 0.0 17 0.0 0.0 0.0 14.732755349271681 0.0 18 0.0 0.0 0.0 16.100359050704967 0.0 19 0.0 0.0 0.0 16.77759290072102 0.0 20 0.0 0.0 0.0 17.459205417870802 0.0 21 0.0 0.0 0.0 18.225472166272585 0.0 22 0.0 0.0 0.0 19.142073152932248 0.0 23 0.0 0.0 0.0 19.96088390693873 0.0 24 0.0 0.0 0.0 20.52719152290043 0.0 25 0.0 0.0 0.0 21.087660916017164 0.0 26 0.0 0.0 0.0 21.58245030212803 0.0 27 0.0 0.0 0.0 22.123945470998628 0.0 28 0.0 0.0 0.0 22.697550865516536 0.0 29 0.0 0.0 0.0 23.25656070292203 0.0 30 0.0 0.0 0.0 23.914820328691945 0.0 31 0.0 0.0 0.0 24.56286306448317 0.0 32 0.0 0.0 0.0 25.063490673439006 0.0 33 0.0 0.0 0.0 25.568496949528562 0.0 34 0.0 0.0 0.0 26.136264121201506 0.0 35 0.0 0.0 0.0 26.744898852789213 0.0 36 0.0 0.0 0.0 27.331640248708293 0.0 37 0.0 0.0 0.0 27.89356919753627 0.0 38 0.0 0.0 0.0 28.45987681349797 0.0 39 0.0 0.0 0.0 29.07143065650816 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTGGT 20 6.986876E-4 45.000004 7 CAAGTGG 20 6.986876E-4 45.000004 12 GCTTGGG 20 6.986876E-4 45.000004 3 CCATGGG 20 6.986876E-4 45.000004 3 CAAGGAT 20 6.986876E-4 45.000004 5 TGGGCAC 20 6.986876E-4 45.000004 6 GGATTCC 20 6.986876E-4 45.000004 8 TGGGACC 40 6.6556822E-9 45.000004 6 AAAGGAT 20 6.986876E-4 45.000004 5 CGTTTGG 20 6.986876E-4 45.000004 2 TCGAACG 20 6.986876E-4 45.000004 1 TTGGGCA 20 6.986876E-4 45.000004 5 CGAACGG 25 3.8523904E-5 45.0 2 GGATCTA 25 3.8523904E-5 45.0 8 CGTCTCT 85 0.0 45.0 35 TAACGTC 85 0.0 45.0 32 ACGTCTC 85 0.0 45.0 34 TTACAGG 25 3.8523904E-5 45.0 2 ATGGGAC 30 2.1356427E-6 44.999996 5 ATCTCTT 30 2.1356427E-6 44.999996 7 >>END_MODULE