##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936370.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68514
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745905946229968	31.0	31.0	34.0	30.0	34.0
2	31.86534139008086	33.0	31.0	34.0	30.0	34.0
3	31.70833698222261	31.0	31.0	34.0	30.0	34.0
4	35.52729369180022	37.0	35.0	37.0	33.0	37.0
5	35.64324079750124	37.0	35.0	37.0	35.0	37.0
6	35.64399976647108	37.0	35.0	37.0	33.0	37.0
7	27.086755991476196	35.0	19.0	37.0	0.0	37.0
8	31.298493738506	35.0	26.0	37.0	17.0	37.0
9	35.921213182707184	39.0	32.0	39.0	32.0	39.0
10	36.54638468050325	37.0	35.0	39.0	32.0	39.0
11	36.88990571270105	39.0	37.0	39.0	33.0	39.0
12	36.919359546953906	39.0	35.0	39.0	33.0	39.0
13	36.75940683655895	39.0	35.0	39.0	33.0	39.0
14	37.81428613130163	40.0	37.0	41.0	33.0	41.0
15	37.96187640482237	40.0	37.0	41.0	33.0	41.0
16	37.919957964795515	40.0	36.0	41.0	33.0	41.0
17	37.852497299821934	40.0	36.0	41.0	33.0	41.0
18	37.76108532562688	39.0	36.0	41.0	33.0	41.0
19	37.643094841930115	39.0	36.0	41.0	33.0	41.0
20	37.68336398400327	39.0	36.0	41.0	33.0	41.0
21	37.59139737863794	39.0	36.0	41.0	33.0	41.0
22	37.61324692763523	39.0	36.0	41.0	33.0	41.0
23	37.54324663572409	39.0	35.0	41.0	33.0	41.0
24	37.51515018828269	39.0	35.0	41.0	33.0	41.0
25	37.43484543305018	39.0	35.0	41.0	33.0	41.0
26	37.310637242023525	39.0	35.0	41.0	33.0	41.0
27	37.204965408529645	39.0	35.0	41.0	32.0	41.0
28	37.191552091543336	39.0	35.0	41.0	32.0	41.0
29	37.17956913915404	39.0	35.0	41.0	32.0	41.0
30	37.08646408033395	39.0	35.0	41.0	32.0	41.0
31	36.957468546574425	39.0	35.0	41.0	31.0	41.0
32	36.715123916279886	39.0	35.0	40.0	31.0	41.0
33	36.59161631199463	39.0	35.0	40.0	30.0	41.0
34	36.422950054003564	39.0	35.0	40.0	30.0	41.0
35	36.30605423709023	39.0	35.0	40.0	30.0	41.0
36	36.149210380360216	39.0	35.0	40.0	30.0	41.0
37	36.00776483638381	38.0	35.0	40.0	30.0	41.0
38	35.886271418980066	38.0	35.0	40.0	29.0	41.0
39	35.8330560177482	38.0	35.0	40.0	29.0	41.0
40	35.62537583559565	38.0	35.0	40.0	27.0	41.0
41	35.61761099921184	38.0	35.0	40.0	27.0	41.0
42	35.491622150217474	38.0	35.0	40.0	27.0	41.0
43	35.37214291969524	38.0	34.0	40.0	27.0	41.0
44	35.12679160463555	38.0	34.0	40.0	25.0	41.0
45	34.98356540269142	38.0	34.0	40.0	24.0	41.0
46	34.79388154245848	38.0	34.0	40.0	24.0	41.0
47	34.646057740023934	38.0	34.0	40.0	23.0	41.0
48	34.528359167469425	38.0	34.0	40.0	23.0	41.0
49	34.34957818839945	37.0	33.0	40.0	23.0	41.0
50	34.05892226406282	37.0	33.0	40.0	22.0	41.0
51	31.714598476223838	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	2.0
11	1.0
12	4.0
13	0.0
14	0.0
15	5.0
16	7.0
17	14.0
18	15.0
19	41.0
20	78.0
21	124.0
22	216.0
23	351.0
24	463.0
25	695.0
26	831.0
27	1003.0
28	1162.0
29	1315.0
30	1585.0
31	2020.0
32	2525.0
33	3519.0
34	5151.0
35	6598.0
36	6518.0
37	9851.0
38	12275.0
39	12137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.66050734156523	21.230697375718833	28.102285664243805	11.006509618472137
2	30.336865458154538	25.25031380447792	27.700907843652395	16.71191289371515
3	30.06538809586362	25.533467612458765	28.6262661645795	15.77487812709811
4	27.902326531803716	25.148144904691012	28.683188837317918	18.266339726187347
5	23.89000788160084	30.110634322912105	27.24260735032256	18.756750445164492
6	25.949440990162593	34.098140526023876	26.22529701958724	13.727121464226288
7	61.26047231222815	27.05286510786117	7.588230142744548	4.098432437166126
8	81.85334384213445	4.940596082552471	8.277140438450536	4.928919636862538
9	75.56995650523982	7.620340368391862	10.991914061359722	5.817789065008611
10	38.716174796391975	32.51160346790437	16.479843535627754	12.292378200075897
11	30.963014858277138	25.273666695857784	26.515748606124294	17.247569839740784
12	29.135651107802786	22.08745657821759	29.76471961934787	19.012172694631754
13	23.110605131797882	29.220305339054793	30.37335435093558	17.295735178211753
14	18.14227749073182	31.650465598271886	30.06392854015238	20.143328370843918
15	15.936888811045918	26.826633972618737	39.11025483842718	18.126222377908167
16	19.381440289575853	26.88647575677964	34.536007239396326	19.196076714248182
17	19.733193215985054	24.727792859853462	30.31643167819716	25.222582245964333
18	20.940245789181773	26.717167294275622	33.01077152114896	19.331815395393644
19	22.176489476603322	27.633768280935282	29.958840528942986	20.230901713518403
20	23.930875441515603	28.4467408120968	29.469889365677087	18.15249438071051
21	21.44379250956009	27.769506962080744	30.744081501590912	20.042619026768254
22	20.029483025367078	26.282219692325654	29.726771170855592	23.961526111451676
23	18.075137928014712	28.23072656683306	30.811221064308025	22.882914440844207
24	20.611845754152437	24.739469305543395	31.546837142773736	23.101847797530432
25	18.45900108007123	28.235105233966777	30.671103716028842	22.634789969933152
26	18.835566453571534	30.0084654231252	29.221764894766032	21.934203228537232
27	19.412090959511925	29.151706220626437	29.9909507545903	21.44525206527133
28	16.955658697492485	27.798698076305573	32.51160346790437	22.734039758297577
29	19.988615465452316	26.30995125083924	31.676737601074233	22.024695682634206
30	20.330151501882828	27.350614472954433	30.50325480923607	21.815979215926674
31	21.613100972064103	27.915462533204895	27.152114896225587	23.319321598505415
32	23.465277169629566	28.23072656683306	28.17672300551712	20.12727325802026
33	21.621858306331553	29.122515106401615	26.283679248036897	22.97194733922994
34	19.65145809615553	26.5405610532154	30.54558192486207	23.262398925767
35	18.710044662404766	27.36521003006685	29.59541115684386	24.32933415068453
36	22.97194733922994	26.578509501707682	28.5065825962577	21.942960562804682
37	19.733193215985054	27.238228683188836	32.361269229646496	20.667308871179614
38	18.36704907026301	29.24657734185714	28.76492395714744	23.621449630732407
39	20.784073328078932	26.922964649560672	31.52056513997139	20.772396882389003
40	21.770732988878187	26.65732551011472	31.38336690311469	20.1885745978924
41	20.124354146597774	26.222377908164756	28.890445748314214	24.762822196923256
42	22.76031176109992	25.4677876054529	28.46863414776542	23.30326648568176
43	22.57494818577225	24.23884169658756	28.60437282891088	24.58183728872931
44	20.17689815220247	26.288057915170622	29.392532912981288	24.14251101964562
45	18.708585106693523	26.622296173044923	29.699039612342006	24.97007910791955
46	22.433371281781824	27.445485594185133	28.59707505035467	21.524068073678375
47	19.08515048019383	26.63397261873486	32.05622208599702	22.22465481507429
48	20.281986163411858	25.41670315555945	29.799748956417666	24.50156172461103
49	20.721312432495548	23.215693143007268	32.326239892576695	23.736754531920486
50	19.829523892926993	25.008027556411825	30.557258370552002	24.605190180109172
51	18.42397174300143	24.818285313950433	29.14732755349272	27.61041538955542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	109.0
2	152.0
3	347.5
4	543.0
5	383.0
6	223.0
7	206.0
8	189.0
9	192.0
10	195.0
11	197.0
12	199.0
13	215.0
14	231.0
15	239.0
16	247.0
17	250.0
18	253.0
19	241.0
20	229.0
21	276.0
22	323.0
23	368.5
24	414.0
25	559.5
26	775.0
27	845.0
28	904.5
29	964.0
30	1195.0
31	1426.0
32	1610.5
33	1795.0
34	1944.5
35	2094.0
36	2233.5
37	2373.0
38	2667.5
39	2962.0
40	3414.0
41	3866.0
42	4358.0
43	4850.0
44	5415.0
45	5980.0
46	6553.5
47	7127.0
48	7258.0
49	7389.0
50	6926.0
51	6463.0
52	5446.0
53	4429.0
54	3798.0
55	3167.0
56	2725.5
57	2284.0
58	2184.5
59	2085.0
60	1830.5
61	1576.0
62	1342.5
63	1109.0
64	876.5
65	644.0
66	520.5
67	397.0
68	357.5
69	318.0
70	252.5
71	187.0
72	136.0
73	85.0
74	72.5
75	41.0
76	22.0
77	21.5
78	21.0
79	13.5
80	6.0
81	6.5
82	7.0
83	6.5
84	6.0
85	6.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	68514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.074875207986686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92182116923729	41.2630995125084
2	9.892934753802935	10.897042940129024
3	4.717231144326072	7.7940274980296005
4	3.0025971272592358	6.6147064833464695
5	1.939895054857688	5.3419739031438835
6	1.383367785021466	4.571328487608372
7	1.1077542799597182	4.270660011092623
8	0.8400911644670588	3.701433283708439
9	0.6439815550961997	3.1920483404851563
>10	1.523824667408703	10.762763814694805
>50	0.018550908994540732	0.8085938640277899
>100	0.007950389569088886	0.7823218612254429
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.3765653735003065	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	169	0.2466649151998132	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	109	0.1590915725253233	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	87	0.12698134687801033	No Hit
GGGTAAGGGACACTTCTGTTGTTAACTGTTCTAATTTTTTTTTGTCTATGT	86	0.12552179116676881	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	85	0.12406223545552734	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	84	0.12260267974428583	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	78	0.11384534547683686	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	74	0.10800712263187084	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19849957672884375	0.0
2	0.0	0.0	0.0	1.058177890650086	0.0
3	0.0	0.0	0.0	1.4055521499255628	0.0
4	0.0	0.0	0.0	1.872609977522842	0.0
5	0.0	0.0	0.0	3.231456344688677	0.0
6	0.0	0.0	0.0	3.841550631987623	0.0
7	0.0	0.0	0.0	4.568409376185889	0.0
8	0.0	0.0	0.0	6.156405990016639	0.0
9	0.0	0.0	0.0	6.7314709402457895	0.0
10	0.0	0.0	0.0	8.36909244825875	0.0
11	0.0	0.0	0.0	10.130776191727238	0.0
12	0.0	0.0	0.0	11.63265901859474	0.0
13	0.0	0.0	0.0	12.185830633155268	0.0
14	0.0	0.0	0.0	12.37411331990542	0.0
15	0.0	0.0	0.0	12.775491140496833	0.0
16	0.0	0.0	0.0	13.619114341594418	0.0
17	0.0	0.0	0.0	14.732755349271681	0.0
18	0.0	0.0	0.0	16.100359050704967	0.0
19	0.0	0.0	0.0	16.77759290072102	0.0
20	0.0	0.0	0.0	17.459205417870802	0.0
21	0.0	0.0	0.0	18.225472166272585	0.0
22	0.0	0.0	0.0	19.142073152932248	0.0
23	0.0	0.0	0.0	19.96088390693873	0.0
24	0.0	0.0	0.0	20.52719152290043	0.0
25	0.0	0.0	0.0	21.087660916017164	0.0
26	0.0	0.0	0.0	21.58245030212803	0.0
27	0.0	0.0	0.0	22.123945470998628	0.0
28	0.0	0.0	0.0	22.697550865516536	0.0
29	0.0	0.0	0.0	23.25656070292203	0.0
30	0.0	0.0	0.0	23.914820328691945	0.0
31	0.0	0.0	0.0	24.56286306448317	0.0
32	0.0	0.0	0.0	25.063490673439006	0.0
33	0.0	0.0	0.0	25.568496949528562	0.0
34	0.0	0.0	0.0	26.136264121201506	0.0
35	0.0	0.0	0.0	26.744898852789213	0.0
36	0.0	0.0	0.0	27.331640248708293	0.0
37	0.0	0.0	0.0	27.89356919753627	0.0
38	0.0	0.0	0.0	28.45987681349797	0.0
39	0.0	0.0	0.0	29.07143065650816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGGT	20	6.986876E-4	45.000004	7
CAAGTGG	20	6.986876E-4	45.000004	12
GCTTGGG	20	6.986876E-4	45.000004	3
CCATGGG	20	6.986876E-4	45.000004	3
CAAGGAT	20	6.986876E-4	45.000004	5
TGGGCAC	20	6.986876E-4	45.000004	6
GGATTCC	20	6.986876E-4	45.000004	8
TGGGACC	40	6.6556822E-9	45.000004	6
AAAGGAT	20	6.986876E-4	45.000004	5
CGTTTGG	20	6.986876E-4	45.000004	2
TCGAACG	20	6.986876E-4	45.000004	1
TTGGGCA	20	6.986876E-4	45.000004	5
CGAACGG	25	3.8523904E-5	45.0	2
GGATCTA	25	3.8523904E-5	45.0	8
CGTCTCT	85	0.0	45.0	35
TAACGTC	85	0.0	45.0	32
ACGTCTC	85	0.0	45.0	34
TTACAGG	25	3.8523904E-5	45.0	2
ATGGGAC	30	2.1356427E-6	44.999996	5
ATCTCTT	30	2.1356427E-6	44.999996	7
>>END_MODULE