##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197179
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892686340837514	31.0	31.0	34.0	30.0	34.0
2	32.00752615643654	33.0	31.0	34.0	30.0	34.0
3	31.723849902880126	33.0	31.0	34.0	30.0	34.0
4	35.5797371931088	37.0	35.0	37.0	33.0	37.0
5	35.71603466900633	37.0	35.0	37.0	35.0	37.0
6	35.70459328833192	37.0	35.0	37.0	35.0	37.0
7	26.938477221205098	35.0	16.0	37.0	0.0	37.0
8	31.17898964899913	35.0	21.0	37.0	17.0	37.0
9	35.92026027112421	39.0	32.0	39.0	32.0	39.0
10	36.646498866512154	38.0	35.0	39.0	33.0	39.0
11	37.05044147703356	39.0	37.0	39.0	34.0	39.0
12	37.07057546696149	39.0	37.0	39.0	34.0	39.0
13	36.971923987848605	39.0	37.0	39.0	33.0	39.0
14	38.05896165413152	40.0	37.0	41.0	33.0	41.0
15	38.1316316646296	40.0	37.0	41.0	33.0	41.0
16	38.10969220860233	40.0	37.0	41.0	33.0	41.0
17	37.99738308846277	40.0	37.0	41.0	33.0	41.0
18	37.872466134831804	40.0	37.0	41.0	33.0	41.0
19	37.72209515212066	40.0	37.0	41.0	33.0	41.0
20	37.76267249554973	40.0	36.0	41.0	33.0	41.0
21	37.697533713022175	40.0	36.0	41.0	33.0	41.0
22	37.745059058013275	39.0	36.0	41.0	33.0	41.0
23	37.71182529579722	39.0	36.0	41.0	33.0	41.0
24	37.65813296547807	39.0	36.0	41.0	33.0	41.0
25	37.543572084248325	39.0	35.0	41.0	33.0	41.0
26	37.446026199544576	39.0	35.0	41.0	33.0	41.0
27	37.324461529878945	39.0	35.0	41.0	33.0	41.0
28	37.28185050132114	39.0	35.0	41.0	32.0	41.0
29	37.22913697706145	39.0	35.0	41.0	32.0	41.0
30	37.15714148058363	39.0	35.0	41.0	32.0	41.0
31	36.97265428874272	39.0	35.0	41.0	31.0	41.0
32	36.8164358273447	39.0	35.0	41.0	31.0	41.0
33	36.66317407026103	39.0	35.0	41.0	30.0	41.0
34	36.406731954214195	39.0	35.0	41.0	30.0	41.0
35	36.25302390213968	39.0	35.0	41.0	30.0	41.0
36	36.068420065017065	39.0	35.0	41.0	29.0	41.0
37	35.98007394296553	39.0	35.0	40.0	29.0	41.0
38	35.75981215038112	39.0	35.0	40.0	27.0	41.0
39	35.70035348591889	39.0	35.0	40.0	27.0	41.0
40	35.48746063221743	38.0	35.0	40.0	26.0	41.0
41	35.38962059854244	38.0	35.0	40.0	25.0	41.0
42	35.30124911882097	38.0	34.0	40.0	25.0	41.0
43	35.33510160818343	38.0	34.0	40.0	25.0	41.0
44	35.147718570436	38.0	34.0	40.0	24.0	41.0
45	35.052591807443996	38.0	34.0	40.0	24.0	41.0
46	34.929789683485566	38.0	34.0	40.0	24.0	41.0
47	34.72005132392395	38.0	34.0	40.0	23.0	41.0
48	34.61399033365622	38.0	34.0	40.0	23.0	41.0
49	34.46526252795683	38.0	33.0	40.0	23.0	41.0
50	34.2329862713575	37.0	33.0	40.0	22.0	41.0
51	32.011446452208396	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	14.0
11	7.0
12	5.0
13	8.0
14	9.0
15	19.0
16	23.0
17	38.0
18	83.0
19	159.0
20	277.0
21	472.0
22	698.0
23	1015.0
24	1538.0
25	2167.0
26	2747.0
27	3164.0
28	3319.0
29	3877.0
30	4399.0
31	5509.0
32	6953.0
33	9016.0
34	13270.0
35	16248.0
36	18267.0
37	28219.0
38	36274.0
39	39361.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50837056684535	20.948985439625922	25.620882548344397	12.921761445184323
2	32.13374649430213	25.681740956187017	25.39824220632014	16.786270343190704
3	29.471698304586187	25.678190882396194	29.353024409293077	15.497086403724536
4	25.97538277402766	24.814001491030993	30.823262112091044	18.387353622850302
5	25.650804598867023	28.545636198580986	27.307674752382354	18.49588445016964
6	25.330283650895886	34.51533885454333	27.026204616110235	13.128172878450545
7	60.19352973693953	26.849715233366638	9.264171133842854	3.6925838958509782
8	80.85749496650251	5.729819098382688	9.543105503121529	3.869580431993265
9	74.51909178969362	7.671202308562271	11.198454196440798	6.611251705303303
10	34.93779763565086	35.441400960548535	16.995724696849056	12.625076706951551
11	31.219348916466767	23.793608852869728	27.3938908301594	17.593151400504112
12	28.71603974054032	21.427738247987868	30.138097870462875	19.71812414100893
13	25.7131844669057	22.668742614578633	33.011121874033236	18.606951044482425
14	21.04230166498461	26.061598851804707	31.866983806591982	21.029115676618705
15	19.62277930205549	24.971726198023116	36.47142951328488	18.93406498663651
16	24.02689941626644	25.854680265139795	30.23952855020058	19.878891768393185
17	24.230775082539218	24.11412980084086	30.691909381830722	20.963185734789203
18	24.667941312208704	24.190202810644134	30.98047966568448	20.16137621146268
19	23.804766227640876	26.972953509247944	28.280902124465584	20.941378138645597
20	24.18563843005594	27.189508010487934	29.628408704780934	18.996444854675193
21	23.805273381039562	27.226023055193505	30.714731284771705	18.253972278995228
22	23.16271002490123	22.829510241962886	30.903392349083823	23.104387384052057
23	21.1396751175328	26.20563041703224	31.731066695743458	20.9236277696915
24	22.5201466687629	24.000527439534636	30.429711074708766	23.049614816993696
25	20.905370247338713	28.404647553745583	28.96454490589769	21.725437293018018
26	20.817125555966914	25.559009833704398	29.284558700470132	24.339305909858556
27	20.97687887655379	25.825265368015867	30.246628697782217	22.951227057648126
28	18.654116310560457	25.567124288083416	31.955228497963777	23.82353090339235
29	21.577348500600976	25.113729149655896	29.477276991971763	23.831645357771365
30	23.53242485254515	24.15064484554643	30.22634256183468	22.09058774007374
31	22.447116579351757	24.790165281292634	30.174612915168453	22.58810522418716
32	24.329162841884784	25.017370003905082	27.855400422965936	22.798066731244198
33	22.715907880656662	24.92963246593197	28.07398353780068	24.28047611561069
34	20.03915224237875	24.755171696783126	32.247856009007045	22.957820051831078
35	20.720259256817407	27.188493703690554	28.39450448577181	23.696742553720224
36	21.493668189817374	27.20269399885383	28.83877086302294	22.464866948305854
37	21.228934115701975	27.50546457787087	29.272387018901608	21.993214287525547
38	20.013794572444326	27.85134319577643	28.285973658452473	23.848888573326775
39	21.120403288382636	25.577267356057188	28.950851764133095	24.351477591427077
40	22.07790890510653	25.662469127036854	29.759254281642566	22.500367686214048
41	19.79318284401483	27.25188787852662	28.755090552239338	24.199838725219216
42	20.524498044923646	26.0088548983411	30.450504364054996	23.016142692680255
43	21.421652407203606	25.398749359718835	29.141034288641286	24.038563944436273
44	21.594084562757697	24.502102150837562	28.999031337008507	24.904781949396234
45	20.71214480243839	23.91025413456808	28.908250878643265	26.46935018435026
46	22.30054924713078	25.95205371768799	28.179471444727888	23.567925590453346
47	19.215535122908626	25.211609755602776	32.105345903975575	23.467509217513022
48	20.05385969094072	25.6518189056644	29.9210362158242	24.373285187570684
49	21.554526597659994	24.385964022537898	30.72335289254941	23.336156487252698
50	19.720659908002375	24.99759102135623	30.51947722627663	24.762271844364765
51	19.98590113551646	24.56752493926838	28.809355965899	26.637217959316157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	181.5
2	289.0
3	1506.0
4	2723.0
5	1855.5
6	988.0
7	857.0
8	726.0
9	723.5
10	721.0
11	705.5
12	690.0
13	687.5
14	685.0
15	667.0
16	649.0
17	634.5
18	620.0
19	637.0
20	654.0
21	753.5
22	853.0
23	954.5
24	1056.0
25	1212.5
26	1579.5
27	1790.0
28	2243.5
29	2697.0
30	3030.5
31	3364.0
32	3810.0
33	4256.0
34	4957.0
35	5658.0
36	6188.0
37	6718.0
38	7255.0
39	7792.0
40	8485.5
41	9179.0
42	10272.0
43	11365.0
44	12536.0
45	13707.0
46	17219.0
47	20731.0
48	19115.5
49	17500.0
50	17027.5
51	16555.0
52	14820.0
53	13085.0
54	11992.0
55	10899.0
56	10087.5
57	9276.0
58	8700.0
59	8124.0
60	7349.5
61	6575.0
62	5783.0
63	4991.0
64	4289.5
65	3588.0
66	3054.5
67	2521.0
68	2098.5
69	1676.0
70	1376.5
71	1077.0
72	951.0
73	825.0
74	653.0
75	392.0
76	303.0
77	231.5
78	160.0
79	130.5
80	101.0
81	65.0
82	29.0
83	23.0
84	17.0
85	17.0
86	17.0
87	12.5
88	8.0
89	6.0
90	4.0
91	5.0
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197179.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.3151958372849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33572890102911	36.395863656880294
2	10.045257078348167	10.108581542659207
3	5.3078791666246685	8.012009392480945
4	3.3857132777615386	6.814113064778704
5	2.348529900918245	5.90833709472104
6	1.7679491185453224	5.337282367797788
7	1.324449909788229	4.664796961136835
8	0.9404199131144733	3.7853929678109735
9	0.6803681043432683	3.0809568970326455
>10	1.8324580943645363	11.714229202907003
>50	0.014111338460452974	0.4949817171199773
>100	0.013103385713277762	1.339392125936332
>500	0.0010079527471752124	0.46505966659735576
>1k	0.0030238582415256375	1.8790033421408974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1529	0.7754375465947185	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	1113	0.564461732740302	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	1063	0.5391040628058769	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	917	0.46505966659735576	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	286	0.14504587202491137	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	283	0.14352441182884587	No Hit
GAATCTATCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	266	0.13490280405114133	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT	243	0.12323827588130581	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	231	0.1171524350970438	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	230	0.1166452816983553	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.1141095147049128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09027330496655324	0.0
2	0.0	0.0	0.0	0.4944745637212888	0.0
3	0.0	0.0	0.0	0.6902357756150502	0.0
4	0.0	0.0	0.0	1.0132924905796257	0.0
5	0.0	0.0	0.0	2.178223847367113	0.0
6	0.0	0.0	0.0	2.6529194285395503	0.0
7	0.0	0.0	0.0	3.2148453942864097	0.0
8	0.0	0.0	0.0	4.241831026630625	0.0
9	0.0	0.0	0.0	4.625238996039132	0.0
10	0.0	0.0	0.0	6.214657747528895	0.0
11	0.0	0.0	0.0	7.060589616541315	0.0
12	0.0	0.0	0.0	8.418746418229121	0.0
13	0.0	0.0	0.0	8.810268842016646	0.0
14	0.0	0.0	0.0	8.966472088812703	0.0
15	0.0	0.0	0.0	9.261128213450723	0.0
16	0.0	0.0	0.0	9.707930357695291	0.0
17	0.0	0.0	0.0	10.221169597168055	0.0
18	0.0	0.0	0.0	10.884019089253927	0.0
19	0.0	0.0	0.0	11.239026468335878	0.0
20	0.0	0.0	0.0	11.67822131160012	0.0
21	0.0	0.0	0.0	12.126037762642067	0.0
22	0.0	0.0	0.0	12.627105320546306	0.0
23	0.0	0.0	0.0	13.101800901718743	0.0
24	0.0	0.0	0.0	13.483180257532496	0.0
25	0.0	0.0	0.0	13.836159023019693	0.0
26	0.0	0.0	0.0	14.157187124389514	0.0
27	0.0	0.0	0.0	14.534002099615071	0.0
28	0.0	0.0	0.0	14.901181160265546	0.0
29	0.0	0.0	0.0	15.27546036849766	0.0
30	0.0	0.0	0.0	15.78312092058485	0.0
31	0.0	0.0	0.0	16.17515049777106	0.0
32	0.0	0.0	0.0	16.56109423417301	0.0
33	0.0	0.0	0.0	16.915087306457583	0.0
34	0.0	0.0	0.0	17.294945202075272	0.0
35	0.0	0.0	0.0	17.720954056973614	0.0
36	0.0	0.0	0.0	18.090161731218842	0.0
37	5.071533986885014E-4	0.0	0.0	18.477626927816857	0.0
38	5.071533986885014E-4	0.0	0.0	18.922907611865362	0.0
39	5.071533986885014E-4	0.0	0.0	19.53656322427845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGGT	30	2.1563283E-6	45.000004	5
GTCGAGG	25	3.8790164E-5	45.0	2
GGCACGT	20	7.019059E-4	45.0	11
CGCATGG	20	7.019059E-4	45.0	2
ACCGATC	20	7.019059E-4	45.0	10
GCACGTG	20	7.019059E-4	45.0	12
TAACGTC	20	7.019059E-4	45.0	32
AGGGTAC	25	3.8790164E-5	45.0	6
TACGGAG	20	7.019059E-4	45.0	1
GCGCATG	20	7.019059E-4	45.0	1
ACGGCGG	20	7.019059E-4	45.0	17
GGTATCC	20	7.019059E-4	45.0	38
GTCAAGC	20	7.019059E-4	45.0	16
GCGATTG	25	3.8790164E-5	45.0	9
TAGGCGA	25	3.8790164E-5	45.0	6
TCGAGGG	45	3.8198777E-10	45.0	3
CGTTTTA	100	0.0	45.0	1
CGTTTTT	545	0.0	41.69725	1
TACGGCT	135	0.0	41.666664	7
GCGATGT	65	0.0	41.53846	9
>>END_MODULE