##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221012
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903113858071055	33.0	31.0	34.0	30.0	34.0
2	32.01937903824227	33.0	31.0	34.0	30.0	34.0
3	31.383200912167666	33.0	31.0	34.0	28.0	34.0
4	35.405036830579334	37.0	35.0	37.0	33.0	37.0
5	35.70658606772483	37.0	35.0	37.0	35.0	37.0
6	35.77075905380703	37.0	35.0	37.0	35.0	37.0
7	27.17884549255244	36.0	19.0	37.0	0.0	37.0
8	31.42007221327349	37.0	26.0	37.0	17.0	37.0
9	36.18731109623007	39.0	32.0	39.0	32.0	39.0
10	36.84684542015818	38.0	37.0	39.0	34.0	39.0
11	37.28289866613578	39.0	37.0	39.0	34.0	39.0
12	37.38692469187193	39.0	37.0	39.0	35.0	39.0
13	37.40442147937669	39.0	37.0	39.0	35.0	39.0
14	38.69497583841601	40.0	38.0	41.0	35.0	41.0
15	38.74929415597343	40.0	38.0	41.0	35.0	41.0
16	38.670764483376466	40.0	38.0	41.0	35.0	41.0
17	38.566308616726694	40.0	38.0	41.0	34.0	41.0
18	38.36405263062639	40.0	38.0	41.0	34.0	41.0
19	38.1470236910213	40.0	37.0	41.0	34.0	41.0
20	38.10203518360994	40.0	36.0	41.0	34.0	41.0
21	38.0686523808662	40.0	36.0	41.0	34.0	41.0
22	38.05486579914213	40.0	36.0	41.0	34.0	41.0
23	37.97851700360162	40.0	36.0	41.0	34.0	41.0
24	37.935225236638736	40.0	36.0	41.0	34.0	41.0
25	37.85063706948039	40.0	36.0	41.0	33.0	41.0
26	37.74186921977087	40.0	36.0	41.0	33.0	41.0
27	37.63495647295169	40.0	36.0	41.0	33.0	41.0
28	37.61665882395526	40.0	36.0	41.0	33.0	41.0
29	37.603840515447125	40.0	36.0	41.0	33.0	41.0
30	37.50608111776736	40.0	36.0	41.0	33.0	41.0
31	37.359794943261	40.0	35.0	41.0	33.0	41.0
32	37.106089262121515	40.0	35.0	41.0	32.0	41.0
33	36.842560584945616	39.0	35.0	41.0	31.0	41.0
34	36.49415416357483	39.0	35.0	41.0	30.0	41.0
35	36.23205527301685	39.0	35.0	41.0	29.0	41.0
36	35.924008651113965	39.0	35.0	41.0	26.0	41.0
37	35.789160769550975	39.0	35.0	41.0	25.0	41.0
38	35.65622681121387	39.0	35.0	41.0	25.0	41.0
39	35.60865925832081	39.0	35.0	41.0	24.0	41.0
40	35.46699726711672	39.0	35.0	41.0	24.0	41.0
41	35.40315457984182	39.0	35.0	41.0	23.0	41.0
42	35.32674243932456	39.0	35.0	41.0	23.0	41.0
43	35.27427470001629	39.0	35.0	41.0	23.0	41.0
44	35.109794943261	39.0	35.0	40.0	22.0	41.0
45	35.01869129278048	39.0	35.0	40.0	22.0	41.0
46	34.929189365283335	39.0	35.0	40.0	22.0	41.0
47	34.81197853510216	38.0	34.0	40.0	21.0	41.0
48	34.748262537780754	38.0	34.0	40.0	20.0	41.0
49	34.59238864858017	38.0	34.0	40.0	20.0	41.0
50	34.3941143467323	38.0	34.0	40.0	20.0	41.0
51	32.47044051906684	36.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	9.0
10	8.0
11	14.0
12	1.0
13	2.0
14	3.0
15	5.0
16	19.0
17	24.0
18	66.0
19	119.0
20	216.0
21	402.0
22	697.0
23	1179.0
24	1937.0
25	3149.0
26	4118.0
27	4335.0
28	4063.0
29	3881.0
30	4116.0
31	4828.0
32	5897.0
33	8209.0
34	11779.0
35	16016.0
36	19290.0
37	31459.0
38	43225.0
39	51930.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.96767596329611	20.202522939930866	25.85289486543717	16.976906231335857
2	35.681320471286625	23.99598211861799	25.75425768736539	14.568439722729988
3	29.123305521872116	23.294662733245254	33.256565254375325	14.325466490507303
4	24.503194396684343	24.990950717608094	34.601741081932204	15.90411380377536
5	23.539898286065917	29.092537961739634	31.498289685627928	15.869274066566522
6	23.75481874287369	33.804499303205255	29.61649141223101	12.824190541690045
7	59.11669954572603	26.303096664434513	11.50209038423253	3.0781134056069352
8	81.03360903480355	4.106564349447089	12.39570702043328	2.4641195953160917
9	74.6529600202704	7.842560584945614	13.505601505800591	3.9988778889834036
10	28.155484770057736	41.883246158579624	19.75775071036867	10.203518360993973
11	25.312652706640364	26.267804463106074	33.868296744068196	14.551246086185365
12	24.59504461296219	23.920420610645575	34.38229598392847	17.10223879246376
13	21.84225290934429	24.118599895028325	37.43914357591443	16.600003619712957
14	19.49170180804662	27.894865437170836	34.086836913832734	18.526595840949813
15	18.21665791902702	27.45009320760864	37.570810634716665	16.762438238647675
16	21.65176551499466	27.02477693518904	34.36238756266628	16.96106998715002
17	21.426438383436196	25.456536296671672	33.964219137422404	19.15280618246973
18	21.804245923298282	25.514904168099466	35.32885092212187	17.35199898648037
19	21.236855917325755	26.92568729299766	33.87417877762293	17.963278012053642
20	21.94360487213364	27.460047418239736	34.1673755271207	16.428972182505927
21	21.799268817982735	26.676832027220243	34.69087651349248	16.833022641304545
22	21.58525328941415	23.73853003456826	35.11936003474924	19.556856641268347
23	19.061408430311477	26.67818941957903	35.38767125766927	18.872730892440227
24	19.32655240439433	25.935243335203516	35.569109369626986	19.169094890775163
25	18.986299386458654	28.696631857093735	33.828932365663405	18.48813639078421
26	18.984037065860676	28.482163864405553	33.8963495194831	18.637449550250665
27	19.36636924691872	27.94418402620672	34.462382133096845	18.227064593777715
28	17.89721825059273	27.0188949016343	36.3116029898829	18.772283857890066
29	18.845130581144915	25.901760990353466	36.194414782907714	19.058693645593905
30	19.98172044956835	26.909398584692234	34.59133440718151	18.517546558557907
31	21.564439939912766	27.16775559698116	32.35027962282591	18.917524840280166
32	21.36671311964961	27.57361591225816	32.79821910122527	18.261451866866956
33	20.50069679474418	27.531989213255386	32.47515971983422	19.492154272166218
34	19.98353030604673	27.627911606609594	33.482344850053394	18.906213237290284
35	20.23464789242213	27.556422275713537	32.58510850089588	19.623821330968454
36	20.90022261234684	28.597994679021955	31.957993231136772	18.543789477494435
37	20.528749570159086	29.52916583714911	31.51955549924891	18.42252909344289
38	20.890268401715744	29.126925234828878	30.237272184315785	19.745534179139597
39	21.368070512008398	28.328326063743148	30.41373319095796	19.889870233290498
40	21.922791522632252	27.627911606609594	31.10012126038405	19.349175610374097
41	20.247769351890398	28.68848750294102	30.885653267695872	20.178089877472715
42	21.24997737679402	28.191229435505765	30.910538794273613	19.6482543934266
43	21.483448862505202	28.201636110256455	30.233200007239425	20.081715019998914
44	21.346804698387416	27.959115342153368	30.60376812118799	20.090311838271223
45	20.54594320670371	27.939659385010767	30.252655964382026	21.2617414439035
46	21.15360251932022	28.726494488987026	29.591605885653266	20.528297106039492
47	19.877653702061426	27.996217399960184	32.00052485837873	20.125604039599658
48	21.011076321647693	27.473168877707998	30.725028505239532	20.790726295404774
49	20.785749190089227	26.579552241507248	31.769315693265526	20.865382875138003
50	20.28396648145802	27.146037319240584	31.48381083380088	21.086185365500516
51	20.28215662497964	26.502180877056446	30.46667149295061	22.748991005013302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	144.0
2	229.0
3	3115.0
4	6001.0
5	4226.5
6	2452.0
7	2154.5
8	1857.0
9	1798.0
10	1739.0
11	1708.5
12	1678.0
13	1641.5
14	1605.0
15	1507.0
16	1409.0
17	1404.5
18	1400.0
19	1397.0
20	1394.0
21	1440.0
22	1486.0
23	1586.0
24	1686.0
25	2005.0
26	2587.5
27	2851.0
28	3412.0
29	3973.0
30	4682.5
31	5392.0
32	6081.0
33	6770.0
34	7629.5
35	8489.0
36	9099.5
37	9710.0
38	10414.0
39	11118.0
40	12232.5
41	13347.0
42	14291.5
43	15236.0
44	16215.0
45	17194.0
46	18606.0
47	20018.0
48	19573.5
49	19129.0
50	18006.5
51	16884.0
52	14844.0
53	12804.0
54	11142.5
55	9481.0
56	8358.0
57	7235.0
58	6354.5
59	5474.0
60	4739.5
61	4005.0
62	3368.5
63	2732.0
64	2206.0
65	1680.0
66	1260.0
67	840.0
68	695.0
69	550.0
70	431.5
71	313.0
72	256.5
73	200.0
74	158.5
75	94.0
76	71.0
77	49.0
78	27.0
79	25.0
80	23.0
81	16.0
82	9.0
83	9.0
84	9.0
85	8.5
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	221012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.006755965700755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4522612117141	33.54623260502876
2	12.370149592553593	12.124418046829732
3	6.176207369766344	9.080276620911052
4	3.8839941717327173	7.613678181852372
5	2.6039566808328094	6.380573480141481
6	1.7973430086770068	5.284917012773547
7	1.2768042385835696	4.380042361536614
8	0.9364494907162503	3.671388133258882
9	0.6826817613014898	3.011041663050319
>10	1.7922879206013071	11.323834930428077
>50	0.014927439386141042	0.5545113974255681
>100	0.010946788883170097	1.2626473244809109
>500	9.95162625742736E-4	0.2618932457498066
>1k	9.95162625742736E-4	1.5045449965328739
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3085	1.3958518089515501	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.24297323222268477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	445	0.20134653321991566	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	444	0.20089406910032032	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	380	0.17193636544622012	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.14162126943333395	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	224	0.10135196278935081	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08325339800553816	0.0
2	0.0	0.0	0.0	0.36966318570937323	0.0
3	0.0	0.0	0.0	0.578249144842814	0.0
4	0.0	0.0	0.0	0.7759759651059671	0.0
5	0.0	0.0	0.0	1.448790110944202	0.0
6	0.0	0.0	0.0	1.8433388232313177	0.0
7	0.0	0.0	0.0	2.226123468408955	0.0
8	0.0	0.0	0.0	3.008886395308852	0.0
9	0.0	0.0	0.0	3.338280274374242	0.0
10	0.0	0.0	0.0	4.276238394295333	0.0
11	0.0	0.0	0.0	5.05990624943442	0.0
12	0.0	0.0	0.0	5.951713029156788	0.0
13	0.0	0.0	0.0	6.286988941776917	0.0
14	0.0	0.0	0.0	6.449423560711636	0.0
15	0.0	0.0	0.0	6.675203156389698	0.0
16	0.0	0.0	0.0	7.080611007547102	0.0
17	0.0	0.0	0.0	7.61587606102836	0.0
18	0.0	0.0	0.0	8.206794201219843	0.0
19	0.0	0.0	0.0	8.550666932112284	0.0
20	0.0	0.0	0.0	8.936166362007492	0.0
21	0.0	0.0	0.0	9.342931605523683	0.0
22	0.0	0.0	0.0	9.828878069969052	0.0
23	4.524641195953161E-4	0.0	0.0	10.281794653683964	0.0
24	4.524641195953161E-4	0.0	0.0	10.661412050024433	0.0
25	4.524641195953161E-4	0.0	0.0	11.005284780916874	0.0
26	4.524641195953161E-4	0.0	0.0	11.31567516695926	0.0
27	4.524641195953161E-4	0.0	0.0	11.652308471938175	0.0
28	4.524641195953161E-4	0.0	0.0	12.013374839375238	0.0
29	4.524641195953161E-4	0.0	0.0	12.38213309684542	0.0
30	4.524641195953161E-4	0.0	0.0	12.81106908222178	0.0
31	4.524641195953161E-4	0.0	0.0	13.179827339691963	0.0
32	4.524641195953161E-4	0.0	0.0	13.568494018424339	0.0
33	4.524641195953161E-4	0.0	0.0	13.924583280545853	0.0
34	4.524641195953161E-4	0.0	0.0	14.304200676886323	0.0
35	4.524641195953161E-4	0.0	0.0	14.704178958608582	0.0
36	4.524641195953161E-4	0.0	0.0	15.088773460264601	0.0
37	4.524641195953161E-4	0.0	0.0	15.441243009429352	0.0
38	4.524641195953161E-4	0.0	0.0	15.80954880277994	0.0
39	4.524641195953161E-4	0.0	0.0	16.249796391146184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0209114E-4	45.0	1
GCTAACG	20	7.0209114E-4	45.0	1
GCCACCG	25	3.8805512E-5	45.0	9
TAGGCGA	30	2.1575215E-6	44.999996	6
CGTTTTT	1525	0.0	44.409836	1
CGTTTTA	245	0.0	43.163265	1
CGAAGGA	75	0.0	42.0	4
AGGCGAT	105	0.0	40.714287	7
CGGTTTA	50	1.0732037E-9	40.5	1
CGGTTTT	195	0.0	40.384617	1
GGCGATT	85	0.0	39.705883	8
GGCGATA	75	0.0	39.0	8
ATTGGGC	35	6.225413E-6	38.57143	4
CGTATGG	30	1.1369408E-4	37.499996	2
AAGCGGC	30	1.1369408E-4	37.499996	22
AAACGGC	30	1.1369408E-4	37.499996	6
TGCGAAG	30	1.1369408E-4	37.499996	2
CCAAGCG	30	1.1369408E-4	37.499996	20
CGGCTAC	30	1.1369408E-4	37.499996	25
TTTGCGG	60	1.546141E-10	37.499996	2
>>END_MODULE